Singer Instruments PIXL LIGHT CURTAIN User manual

QUICK START GUIDE v1.1
For more support
resources visit
bit.ly/PIXLhelp
LIGHT CURTAIN

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·The blade is sharp! Be careful when handling the PIXL blade. Always
follow the instructions on page 42 when changing blades.
·Hand feed the PickupLine™ carefully. When feeding a PickupLine™
into the machine, if you feel resistance, pull back slightly and re-feed.
Forcing the PickupLine™ through will cause damage to the machine.
·Remove lids when placing plates into the PIXL. Lids need to be
manually removed. Failure to do so will cause damage to the picking
arm.
·The nozzle is hot! Be careful when removing the nozzle as it can get
quite hot.
·The feed button is disabled. The feed button is disabled on all PIXL
machines. The retract button is fully functional during the change
PickupLine maintenance routine.
·Light curtain. The light curtain PIXL is sold without a door to allow
access for robotic arms and 3rd party integrations. The light curtain
is a safety feature that will stop any potentially dangerous moving
components if the beam is broken. UV sterilisation is disabled on
Light curtain PIXLs as there is no physical barrier between the UV
bulbs and the user. Do not attempt to tamper with or override the
light curtain sensors. Please report any faults with the light curtain,
or any of the safety mechanisms immediately to your technical
support team.
·Do not use >2% agar when using agar piercing. Agar piercing allows
the PickupLine tip to pierce the surface of the agar. Using >2% will
reduce effectiveness of this feature.
READ BEFORE YOU START!
Join our Discovery community and help us to make the product
features YOU want.
bit.ly/CustomerDisco

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IMPORTANT COVID-19
INSTALLATION NOTICE
We are starting to perform in-person installations again! But for those
we cant make it to, never fear! To get you up and running as quickly
as possible we have instead included detailed instruction on how to
self-install the PIXL (see page 12). We ask that you contact our
Tech Support Team to let them know when you’re planning to perform
the self-installation so they can be on hand should you require any
assistance: [e-mail]
Once the installation is complete, please fill-in the online Self-
Installation Form: http://bit.ly/Selfinstallation
You will be asked to create an account before you start. You can also use
this form to report any issues encountered during installation.
NOTE: Completion of this form will activate the start of your warranty
period. Failure to complete the form may affect the support we are able
to offer during the warranty period.
Thanks for being awesome!

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2Read before you start!
4Introduction
5Anatomy & features
11 Unboxing & assembly
15 PIXL Light Curtain features
FULL USER GUIDE AVAILABLE HERE:
bit.ly/PIXLguide
PIX L™
INTRODUCTION
An ultra-reliable and super-easy-to-use microbial colony picker.
It automates imaging, colony recognition, colony selection, and
picking; from Petri dishes to multiwell plates.
The information in this guide relates to software version:
2.22.0719.1

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ANATOMY & FEATURES
Light curtain
FRONT
Kinematic
nozzle
Emergency stop Dump drawer
Pinpoint™ picking arm

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ANATOMY & FEATURES
CAMERA & LIGHTING
PL ATE BAYS
6-channel SpectraStar™
illumination (left and right)
Anvil*
Blade*
*The Anvil and Blade
together are referred
to as the Cleaver
4 x Target bays
Nozzle
calibration
sensor
Removable
source bay
Colour-coded
bay labels
HD camera with filter tray

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ANATOMY & FEATURES
PickupLine™
compartment
Connections:
1 x HDMI
1 x USB 3.0
2 x USB 2.0
2 x RJ45
Power switch
RIGHT
LEFT

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ANATOMY & FEATURES
PickupLine™ reel
Reel cover
cap
Reel backing
plates
Spring catch
Entry
guide
Tension lever
PickupLine™ retract button
Disabled button
PICKUPLINE™ COMPARTMENT

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ANATOMY & FEATURES
ACCESSORIES
UNDER BLACKOUT SCREEN
2 x UV bulbs
1x 2mm, 2x 2.5mm, 1x
3mm & 1x 4mm hex keys
Power cable
Barcode scanner
(optional extra)
PickupLine™
Ethernet cable
Dump
drawer
Dell 22 Monitor:
P2219H
150mm petri
source bay
(optional extra)
Camera filters
(optional extra)
Mouse
PlusPlate
source bay
Anvil &
Blade
Kinematic nozzle
(plus optional extras)
90mm petri
source bay
(optional extra)
Keyboard

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ANATOMY & FEATURES
Selection options
Current selection title
Power
options
SOFTWARE
Go to home screen
Previous stage
Next stage
Current stage
Project stages
Stage objective
Summary of
current project
Live preview
of selection
Help
menu
Software
settings
Edit
menu
Editable options for
current stage

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2
1
· Use 2mm Hex Key to undo Front Screw (1), then
remove Securing Rod (2).
· To remove the Red Transit Bracket follow steps 3 and
4 below.
· Use 4mm Hex Key to undo the 4 Bracket Screws
behind the transit bracket and carefully remove.
· Remove Packing Crate Side Panels and locking nuts
(1).
· Carefully lift the PIXL and Place on a sturdy desk (2).
· HEAVY: Minimum of 4 people recommended
UNBOXING & ASSEMBLY
1
3
5
2
4
6
1
2
1
2
· Install the Source Bay by sliding the right side under
the Cleaver Tabs, then lowering the left side onto the
magnetic base.
· Ensure the Dump Drawer is installed.

12
EF
AB
· Plug in the provided Screen cables: Power (A) and
HDMI (B).
· Set up the Screen Stand according to the
manufacturer’s instructions.
A
CD
· For remote access and software upgrades, connect
an Ethernet Cable to the right RJ45 Socket (D).
· For internal network access, connect an Ethernet
Cable to the left RJ45 Socket (C).
· Connect the Screen to the PIXL: HDMI (A).
·If using the Bluetooth mouse/keyboard, place the
Bluetooth dongle into an available USB slot.
UNBOXING & ASSEMBLY
8
10
11 12
7
9
· Plug in the PIXL Power Cable (E) and press the Power
Switch (F).
· The Screen will also turn on.
· Turn on the Screen Power Switch.

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· After initialising follow on-screen instructions for
PickupLine™ installation.
· Open the PickupLine™ Compartment.
· Remove the Reel Backing Plates.· Remove the Reel Cap Cover.
UNBOXING & ASSEMBLY
13
15
17
14
16
18
· Fit the Reel Backing Plates into new the PickupLine™
Reel.
· Remove the new PickupLine™ Reel from it’s sterile
packet and dispose of the Securing Tape/Velcro.

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13
2
UNBOXING & ASSEMBLY
· Fit the new PickupLine™ Reel ensuring it feeds in a
clockwise direction.
· Replace the Reel Cap Cover.
· Carefully lift the Tension Lever and rest it on the
Spring Catch as shown.
· Feed the PickupLine™ until it protrudes 10mm from
the Kinematic Nozzle.
· Gently feed the PickupLine™ by hand through the 3
Entry Guides. The PickupLine™ is sterilised at the
nozzle but for extra sterility gloves should be worn.
· If you meet resistance, pull back slightly and re-feed.
19
21
20
22

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TROUBLESHOOTING
PROBLEM
Plate guides could not be detected
The PickupLine™ hits the top plate as the picking
arm moves between positions
The PIXL is missing colonies.
Blades wear down quickly. Please correctly follow instructions for changing the blade in the
PIXL software.
For more support resources visit
bit.ly/PIXLhelp
SOLUTION
Ensure that a source plate holder is installed into the PIXL.
Ensure that the plate dimensions in the software match that
of the plate being used. If the plate type is unsupported please
contact [e-mail] and we’ll
make a new dimensions file for the plate.
Ensure that the nozzle has been calibrated by running the
‘Calibrate Nozzle’ routine in the software. Ensure that the Detect
automatically’ option is selected for ‘Agar Depth’.

Roadwater
Watchet
Somerset
TA23 0RE
UK
[phone] (tel)
[phone] (fax)
[e-mail]
singerinstruments.com
DISCLAIMER
At Singer Instruments, we are constantly
seeking to improve our products and adapt
them to the requirements of modern research
techniques and testing methods. This involves
modification to the mechanical structure and
optical design of our instruments. Therefore, all
descriptions and illustrations in this user guide,
including all specifications are subject to change
without notice.
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HELPFUL TIPS AND TUTORIALS!