NuAire LabGard E NU-543-300 User manual

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LabGard® ES Energy Saver
Class II, Type A2 Laminar Flow
Biosafety Cabinet
Models
NU-543-300/400/500/600
Bench/Console
Operation and Maintenance Manual
July, 2021
Revision 2
NU-543-300 Series E
Nu-543-400/500/600 Series D
Manufactured By:
NuAire, Inc.
2100 Fernbrook Lane
Plymouth, MN 55447
Toll-Free: 1-800-328-3352
In Minnesota: (763)-553-1270
Fax: [phone]

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Congratulations!
You have just purchased one of the finest Laminar Flow Biosafety Cabinets available. With proper care,
maintenance (certification), and laboratory procedure, this cabinet will give you years of product and
personnel protection from particulate contaminants as prescribed in NSF/ANSI 49. Please read this manual
carefully to familiarize yourself with proper installation, maintenance and operation of the cabinet. Other
reference and guideline materials are available through the following web sites;
www.hc-sc.gc.ca
www.cdc.gov/od/ohs/
www.absa.org
www.cabs-acsb.ca
www.ebsaweb.eu
www.inspection.gc.ca
www.who.int
www.biosafety.be
www.hse.gov.uk
www.nsf.org
www.cetainternational.org
www.nuaire.com

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About this Operation and Maintenance Manual
The information contained in this manual is intended to reflect our current production model along
with the more frequently purchased options. Any unique additions, modifications, or shop drawings are
appended in the back flap of this manual, along with any modifications and/or additions to procedures as
outlined in this manual. A copy of the original factory test report is also appended to this manual. In case this
manual and/or test report is lost or misplaced, NuAire retains a copy in our files. A replacement copy can be
obtained by calling or writing NuAire, Inc. stating the model number and serial number and a brief description
of the information desired.

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LabGard® ES Energy Saver Class II, Type A2 Laminar Flow Biosafety Cabinet
Models NU-543-300/400/500/600 Operation and Maintenance Manual
Table of Contents
Section No. 1.....................................................General Information
Section No. 2.....................................................Models and Features
Section No. 3.....................................................Warranty
Section No. 4.....................................................Shipments
Section No. 5.....................................................Installation Instructions
5.1..................................................................... Location
5.2..................................................................... Set-up Instructions
5.3..................................................................... Testing Methods and Equipment
Section No. 6.....................................................Operating the NU-543
6.1..................................................................... FlowGard™ Control System
6.2..................................................................... Operating Guidelines
6.3..................................................................... Operating Sequence
6.4..................................................................... Ergonomics
6.5..................................................................... Cleaning Procedures
6.6..................................................................... Hazardous Drug Contamination Procedures
Section No. 7.....................................................General Maintenance
7.1..................................................................... Decontamination
7.2..................................................................... Fluorescent Lamp Bulb Replacement
7.3..................................................................... HEPA Filter/Motor Replacement
7.4..................................................................... Sliding Window Replacement and Manual Adjustment
7.5..................................................................... Airflow Control System Setup and Calibration
7.6..................................................................... HEPA Filter Leak test
7.7..................................................................... Airflow Smoke Pattern Test
7.8..................................................................... Site Installation Assessments
7.9..................................................................... Cleanliness Classification Test for Pharmacy Application
7.10................................................................... Main Control Board Description and Replacement
7.11................................................................... Digital Airflow Sensor Description and Replacement
Section No. 8 ....................................................Error Messages, Troubleshooting and Option-Diagnostics
Section No. 9.....................................................Remote Contacts
Section No. 10...................................................Optional Equipment
10.1................................................................... Ultraviolet Light
Section No. 11...................................................Electrical/Environmental Requirements
Section No. 12...................................................Disposal and Recycle
Manual Drawings
ACD-16371 ......................................NU-543 Airflow Schematic
BCD-16372 ......................................NU-543-300 Specification Drawing
BCD-16373 ......................................NU-543-400 Specification Drawing
BCD-16374 ......................................NU-543-500 Specification Drawing
BCD-16375 ......................................NU-543-600 Specification Drawing
BCD-16300 ......................................Drain Valve Installation
BCD-16377 ......................................Front Label
BCD-16509 ......................................NU-500 Series Auto Decontamination Set-Up
Assembly Drawings
BCD-16633 ......................................Base Stand Assembly
BCD-16301 ......................................Control Center and Front Decorative Panel Assembly
BCD-16376 ......................................Sliding Window Assembly and Adjustment
BCD-16299 ......................................Base Cabinet Assembly
Electrical Schematics
BCD-16378 (sheet 1 and 2) .............NU-543-300/400/500/600 Electrical Schematic

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LabGard® ES Energy Saver
Class II, Type A2 Laminar Flow
Biosafety Cabinet
Models
NU-543-300/400/500/600
Manufactured by:
NuAire, Inc. - Plymouth, Minnesota, U.S.A.
1.0 General Information
1.1 Description
The LabGard® ES Model NU-543 Laminar Flow Biosafety Cabinet (LFBSC) is a bench/table top model, optionally available
with a base support stand, for operation as a console model. The LabGard® ES model NU-543 utilizes an Energy Saver DC
ECM motor optimally determined forward curved fan for each model size/width to maximize both energy efficiency and
filter loading capacity. The Energy Saver ECM motor is controlled to airflow setpoints by the FlowGard™ control system via
an integrated solid-state DC motor controller based on feedback algorithm to provide automatic compensation (constant
volume control) for both filter loading and line voltage variances. Airflow velocity is monitored with the Intelliflow digital
dual thermistor airflow sensor.
The Laminar Flow Biosafety Cabinet, (LFBSC) is a product resulting from the development of the "laminar flow" principle
(see Airflow Schematic) and the application of environmental controls as required in the field of biological research or
chemical containment. The LFBSC, when used with proper technique, is an effective primary engineering control in
obtaining the optimum product quality while reducing the potential for exposure of both product and personnel to airborne
biological or particulate chemical agents in low to moderate risk-hazard research and drug preparation or product
operations, as prescribed by the Center for Disease Control (CDC) Atlanta, Georgia.
The NU-543 bench LFBSC meets the requirements of a Class II, since the cabinet conforms to the following requirements:
1. Maintains a minimum inflow velocity of 100 LFPM (.51mps) through the work access opening.
2. Has HEPA filtered downflow air that is mixed with the inflow air from a common exhaust plenum.
3. Discharges a percentage of air to the outside atmosphere after HEPA filtration.
4. Has all biologically contaminated ducts and plenums under negative pressure or surrounded by negative pressure.
Cabinets used for work with minute quantities of non-flammable or explosive volatile toxic chemicals and tracer amounts of
radionuclides required as an adjunct to microbiological studies must be exhausted through properly functioning exhaust
canopies.

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1.2 Safety Instructions
These safety instructions describe the safety features of the LabGard® ES Model NU-543 LFBSC.
The safety cabinet has been manufactured using the latest technological developments and has been thoroughly tested
before delivery. However, the cabinet may present potential hazards if it is not installed and used as instructed for its
intended purpose or is used outside of operating parameters. Therefore, the following procedures must always be
observed:
•The safety cabinet must be operated only by trained and authorized personnel.
•For any operation of this cabinet, the operator must prepare clear and concise written instructions for operating
and cleaning, utilizing applicable safety data sheets, plant hygiene guidelines, and technical regulations, in
particular.
oWhich decontamination measures are to be applied for the cabinet and accessories
oWhich protective measures apply while specific agents are used
oWhich measures are to be taken in the case of an accident
•Repairs to the device must be carried out only by trained and authorized expert personnel.
•Keep these operating instructions close to the cabinet so that safety instructions and important information are
always accessible.
•Should you encounter problems that are not detailed adequately in the operating instructions, please contact your
NuAire Representative of NuAire technical Services.
1.3 Explanation of Symbols
CAUTION used without the safety alert symbol indicates a potentially
hazardous situation which, if not avoided, may result in property damage.
Potential electrical hazard, only qualified person to access.
Note: Used for important information.
Biohazard
Ground, Earth
CAUTION

Chemical Hazard
Flammable Hazard
Hazardous Gases! Personal
Protection Equipment Required.
Lead Free
Safety alert symbol indicates a potentially hazardous
situation which, if not avoided, could result in death of
serious injury.
Safety alert symbol indicates a potentially hazardous
situation which, if not avoided, may result in minor or
moderate injury.
WARNING
!
CAUTION
!

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2.0 Models and Features
The model NU-543, LabGard® ES, Class II, Type A2 Laminar Flow Biosafety Cabinet is manufactured in
four sizes: 3 ft. (.9m), 4 ft. (1.2m) , 5 ft. (1.5m) , and 6 ft. (1.8m).

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3.0 Warranty
NuAire, Inc. warrants Class II, Type A2 w/ECM Motor (LabGard® ES) that it will repair F.O.B. its factory or furnish without
charge F.O.B. its factory a similar part to replace any material including HEPA filters in its equipment within 60 months after
the date of sale if proven to the satisfaction of the company to have been defective at the time it was sold; provided that all
parts claimed defective shall be returned, properly identified to the company at its factory, charges prepaid. Factory
installed equipment or accessories are warranted only to the extent guaranteed by the original manufacturer, and this
warranty shall not apply to any portion of the equipment modified by the user. Claims under this warranty should be
directed to NuAire, Inc. setting forth in detail the nature of the defect, the date of the initial installation and the serial and
model number of the equipment.
This warranty shall not apply to any NuAire product or part thereof which has been subject to misuse, abuse, accident,
shipping damage, improper installation or service, or damage by fire, flood or acts of God. If the serial number of this
product is altered, removed or defaced as to be illegible, the Warranty shall be null and void in its entirety.
The warranty is for the sole benefit of the original purchaser and is not assignable or transferable. Prior to returning any
item, for any reason, contact NuAire for a Return Authorization Number. This number must accompany all returns. Any
product shipped to NuAire without this number will be returned to you as refused shipment or collect freight.
4.0 Shipments
NuAire takes every reasonable precaution to assure that your LabGard® ES cabinet arrives without damage. Motor carriers
are carefully selected and shipping cartons have been specially designed to insure your purchase. However, damage can
occur in any shipment and the following outlines the steps you should take on receipt of a NuAire LabGard® ES cabinet to
be sure that if damage has occurred, the proper claims and actions are taken immediately.
4.1 Damaged Shipments
4.1.1 Terms are factory, unless stated otherwise. Therefore, it is important to check each shipment before acceptance.
4.1.2 If there is visible damage, the material can be accepted after the driver makes a notation on the consignee's copy
of the freight bill. Then an inspection must be made to verify the claim against the carrier. This inspection is the
basis of your filing the claim against the carrier.
4.1.3 If concealed damage is found, it is absolutely necessary to NOTIFY THE FREIGHT AGENT AT ONCE, and request an
inspection. Without this inspection, the transportation company may not accept a claim for loss or damage. If the
carrier will not perform the inspection, an affidavit must be prepared stating that he was contacted on a certain
date and that he failed to comply with the request. This along with other papers in the customer's possession will
support the claim.

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5.0 Installation Instructions
5.1 Location
Within the laboratory, pharmacy, etc., the ideal location of the Biosafety cabinet is away from personnel traffic lanes, air
vents (in or out), doors and/or any other source of disruptive air currents.
Suggested Cabinet Location in Laboratory
THE EXHAUST FILTER AREA IS ESPECIALLY SUSCEPTIBLE TO DISRUPTIVE AIR CURRENTS FROM AIR VENTS. The exhaust
sensor for the Electronic Airflow Control System is located just above the exhaust HEPA filter and if disruptive air currents
are present, the exhaust sensor could be influenced by them, and indicate disruptive readings on the front panel display.
If drafts or other disruptive air currents exceed the inflow velocity of the cabinet through the access opening, the potential
exists for contaminated air to exit or enter the work zone area of the cabinet. It depends on the severity of the air current.
REMEMBER: A BIOSAFETY CABINET IS NO SUBSTITUTE FOR GOOD LABORATORY TECHNIQUE.
Where space permits, a clear 6" (152mm) area should be permitted on each side of the cabinet for maintenance purposes.
The electrical outlet into which the cabinet is connected should be readily accessible for maintenance purposes. Do not
position the cabinet to prevent access to the power cord. The power cord plug serves as the disconnect and should
remain readily accessible. If the outlet is inaccessible, such as a conduit (hardwired) connection, then an appropriate
warning label should be applied near the cabinets on/off switch to indicate the circuit breaker on the power distribution
panel should be used. A MINIMUM CLEARANCE OF 6" (152MM) IS REQUIRED FROM THE TOP OF THE CABINET TO THE
CEILING FOR PROPER VENTILATION OF THE EXHAUST EFFLUX.
If this cabinet is used in a pharmacy application, it is strongly recommended (Per OSHA, NIOSH and ASHP) that the cabinet
be exhausted to the outside. In addition, if this cabinet is used in microbiological application with minute quantities of
volatile toxic chemicals and trace amounts of radionuclides, per CDC/NIH and NSF, it is strongly recommended that the
cabinet be exhausted to the outside. NuAire offers a canopy type of exhaust transition, which will capture the exhaust
efflux from the cabinet.
Canopy, Thimble or Air Gap Exhaust Transitions (with and without integral fan)
Note: The Exhaust System should be fitted with a backdraft damper to prevent reversing of airflow in the system.
NuAire only recommends a canopy or thimble transitions for exhausting applications. See separate instruction sheets for a
discussion of exhaust transitions and installation requirements.


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5.2 Set-Up Instructions
Remove outer shipping protection (carton or crating). The cabinet is fastened to the base skid and it is usually the best
procedure to leave the skid in place until the cabinet is located in its approximate position to facilitate ease in handling. It
can then be removed from the skid by removing the banding, bolts and screws holding the cabinet to the skid. It may be
necessary to remove the control center in order to gain passage through a doorway. It can easily be removed by following
the instructions on drawing BCD-16301.
It is recommended that no less than two people are present using a
lifting system for placement of the cabinet onto the base stand.
It is not recommended to manually lift the cabinet onto the base stand.
5.2.1 Base Stand Assembly
The base stand is typically shipped knocked down in a separate carton and is assembled per drawing BCD-16385
Once assembled; place the stand in approximate position (ready for cabinet placement onto the base stand). Now
lift the cabinet on top of the base stand and bolt the base stand to the cabinet using two 3/8" - 16 x 3/4" bolts and
washers provided for the front base stand tabs and two 1/4" acorn nuts for the rear weld studs. Place the cabinet
in the desired location.
5.2.2 Leveling
Using a level placed on the work tray, first adjust the leg levelers end to end and then front to back. The NSF
approved leg levelers provide a 3/4" (20mm) adjustment.
5.2.3 Bench Installation (BCD-16300)
Place the cabinet on the bench with approximately a 2" (51mm) overhang clearance for installation of the drain
valve. Place the cabinet in desired location and using RTV caulk, seal all around the base of the cabinet and the
bench. This provides a tight seal to prevent bench spills from migrating under the cabinet.
If the drain valve is desired, (NOTE, CHECK WITH YOUR SAFETY PERSONNEL FOR REGULATORY REQUIREMENTS OF
DRAIN VALVE INSTALLATION) remove the handle from the valve stem to gain clearance for valve body rotation.
Add Loctite 242 (furnished) to the threads and rotate valve body until secure, with the valve stem (for handle) on
the left side. Re-install handle to valve stem. Adjust the cabinet on bench to provide a 2" (51mm) overhang and
seal the interface of the bench and cabinet, using RTV caulk as above.
5.2.4 Gas Service
NuAire doesn't recommend the use of natural gas within the LFBSC, but if gas service is determined to be
necessary for the application, appropriate safety measures must take place. All NuAire LFBSC's have precautionary
warning labels that say the following:
Use of explosive or flammable substances in this cabinet
should be evaluated by your appropriate safety personnel.
Once the determination has been made by the appropriate safety personnel, the application of natural gas must
be performed in accordance to national, state and local codes. IT IS ALSO STRONGLY RECOMMENDED THAT AN
EMERGENCY GAS SHUTOFF VALVE BE PLACED JUST OUTSIDE THE LFBSC ON THE GAS SUPPLY LINE.
All NuAire’s LFBSC’s meet the safety requirements of UL and CSA for Laboratory Equipment. To comply with these
safety requirements, NuAire uses only certified gas valves. If external piping is required, only black pipe is used for
this application.
As previously stated NuAire doesn't recommend the use of natural gas within the LFBSC and ASSUMES NO
RESPONSIBILITY FOR ITS USE. USE AT YOUR OWN RISK. The Bunsen burner flame within the LFBSC not only
contributes to heat build-up; it also disrupts the laminar air stream, which must be maintained for maximum
efficiency. IF THE PROCEDURE DEMANDS USE OF A FLAME, A BUNSEN BURNER WITH ON DEMAND IGNITION IS
STRONGLY RECOMMENDED. DO NOT USE CONSTANT FLAME GAS BURNERS. During use, the Bunsen burner
should be placed to the rear of the workspace where resulting air turbulence will have a minimal effect.
CAUTION
!
CAUTION
!

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5.2.5 Plumbing Services
Service ball valves with the type of service specified by the removable button on the handle are located in the
work zone. The service ball valves are not recommended for pressure over 75 p.s.i. (5.2 BAR). Reducing valves
should be installed external to the cabinet if necessary. Service ball valves should never be used for flammable
gasses or oxygen service. A special needle valve for oxygen service or certified valve is required and available upon
request.
External connection is to 3/8 inch NPT coupling in the inner sidewalls. Connection to plant utilities should be made
with proper materials for the individual service and according to national and/or local codes. Observe all labels
pertaining to the type of service and operating pressure.
5.2.6 Electrical Services
The NU-543 series Biosafety Cabinets may be "hardwired" (optional) or plugged into an outlet with protective
earthing connection with the standard power cord. The cabinet requires 115 VAC, 60 Hz, single phase (current
rating varies per cabinet size, reference Electrical/Environmental Requirements). It is recommended that power to
the cabinet, whether hardwired or plug connected, be on its own branch circuit, protected with a circuit breaker at
the distribution panel near the cabinet. A surge protector is strongly recommended if you are experiencing power
related faults.
NOTE: THIS CABINET CONTAINS ELECTRONIC BALLASTS FOR THE FLUORSCENT LIGHTING. ELECTRONIC
BALLASTS OPERATE WITH HIGH INRUSH CURRENT. IT IS NOT RECOMMENDED TO USE THIS
PRODUCT WITH GROUND FAULT CIRCUIT INTERRUPTERS (GFCI’S) BECAUSE THE BALLASTS MAY
CAUSE THE GFCI TO TRIP.
5.2.7 Final Assembly
NOTE: Remove the protective cardboard cover over the exhaust HEPA filter.
Remove the protective cardboard cover over the exhaust HEPA filter located under the protective screen if in
place. The exterior surface and viewing glass are easily cleaned with any mild household detergent cleaner using a
soft cloth. Harsh chemicals, solvent-type cleaners and abrasive cleaners should not be used.
Do not attempt to clean the HEPA filter media. Cabinet interior walls or work surface are easily cleaned with any
mild household detergent cleaner using a soft cloth. Turn the cabinet on and let it operate for 60 minutes before
using it as a LFBSC.



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5.3 Testing Methods and Equipment
After installation and prior to use, NuAire recommends that the cabinet be tested or commissioned to factory standards.
As part of testing, the certifier should go through the following initial checklist to assure all aspects of the LFBSC installation
are complete and ready for testing.
•Review product installation
- Exhaust connection, if present
- Damper valve installed correctly with label toward front, if present
- LFBSC base stand level
•Verify airflow sensor shroud is in place
- Exhaust flow
- Verify user menu language desired
•Verify configuration motor type and size selection for specific model
•Perform LFBSC certification
- At a minimum, the following tests should be performed:
•HEPA filter leak test
•Downflow velocity test
•Inflow velocity test
•Airflow smoke patterns
•Site installation assessment tests
The testing methods and equipment required are specified on the factory inspection report included with this manual (see
insert in back cover).
NOTE: IT IS RECOMMENDED THAT THESE TESTS BE PERFORMED BY A QUALIFIED TECHNICIAN WHO IS FAMILIAR
WITH THE METHODS AND PROCEDURES FOR TESTING BIOSAFETY CABINETS (SEE INSERT).
NOTE: AFTER THE INITIAL CERTIFICATION, NUAIRE RECOMMENDS THAT THE CABINET BE RECERTIFIED AT A
MINIMUM OF AN ANNUAL BASIS AND AFTER EVERY FILTER CHANGE, MAINTENANCE ACTION, OR ANY
TIME THE OPERATOR FEELS IT IS NECESSARY.
Note that the LabGard® ES cabinets, filters and seals provide premium performance; Quality Control in both design and
manufacturing assure superior reliability. However, protection to both product and operator is so vital that installation
testing to the performance requirements should be accomplished as stated to ensure Biosafety established by the factory
standards.



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LabGard® ES Energy Saver Class II Type, A2 Laminar Flow Biosafety Cabinet
Models NU-543-300/400/500/600
Catalog Number
Catalog Number
NU-543-300
Nominal 3 foot (0.9m)
NU-543-400
Nominal 4 foot (1.2m)
NU-543-500
Nominal 5 foot (1.5m)
NU-543-600
Nominal 6 foot (1.8m)
Performance Specifications
1. Personal Protection
2. Product Protection
NSF/ANSI 49
NSF/ANSI 49
NSF/ANSI 49
NSF/ANSI 49
NSF/ANSI 49
Class II, Type A2
Class II, Type A2
Class II, Type A2
Class II, Type A2
Style of Cabinet
Bench top/console
w/base stand/storage
cabinet
Bench top/console
w/base stand/storage
cabinet
Bench top/console
w/base stand/storage
cabinet
Bench top/console
w/base stand/storage
cabinet
Cabinet Construction
All welded stainless steel
16/18GA, Type 304
pressure tight design
All welded stainless steel
16/18GA, Type 304
pressure tight design
All welded stainless steel
16/18GA, Type 304
pressure tight design
All welded stainless steel
16/18GA, Type 304
pressure tight design
Diffuser for Air Supply (Metal)
Non-flammable
Non-flammable
Non-flammable
Non-flammable
HEPA Filter Seal Type:
Supply Filter-99.99% Eff. on 0.3 microns
Exhaust Filter-99.99% Eff. on 0.3 microns
HEPEX Seal
Neoprene, Spring-loaded
HEPEX Seal
Neoprene, Spring-loaded
HEPEX Seal
Neoprene, Spring-loaded
HEPEX Seal
Neoprene, Spring-loaded
Fumigation : per NIH/NSF Procedures
Yes
Yes
Yes
Yes
Standard Services:
Service Coupling (3/8 inch NPT)
Service Coupling (3/8 inch NPT)
Gas Valve/Service Coupling (3/8inch NPT)
Duplex Outlet
One Right Sidewall
Two Left Sidewall
One Right Sidewall
Two Backwall
One Right Sidewall
Two Left Sidewall
One Right Sidewall
Two Backwall
One Right Sidewall
Two Left Sidewall
One Right Sidewall
Two Backwall
One Right Sidewall
Two Left Sidewall
One Right Sidewall
Two Backwall
Optional Services:
Gas Cocks 3/8" NPT
Ultraviolet Light
Standard/Cup Sinks
Up to 3 ea. Sidewall
One, Backwall
Left or Right Work
Surface
Up to 3 ea. Sidewall
One, Backwall
Left or Right Work
Surface
Up to 3 ea. Sidewall
One, Backwall
Left or Right Work
Surface
Up to 3 ea. Sidewall
One, Backwall
Left or Right Work
Surface
Cabinet Size Inches (mm):
Height (Fully Assembled)
Height (Minimum for Transport)
Width
Depth with Armrest Removed
60 7/8 (1546)
59 (1499)
41 5/8 (1057)
31 7/16 (799)
60 7/8 (1546)
59 (1499)
53 5/8 (1362)
31 7/16 (799)
60 7/8 (1546)
59 (1499)
65 5/8 (1669)
31 7/16 (799)
60 7/8 (1546)
59 (1499)
77 5/8 (1972)
31 7/16 (799)
Work Access Opening Inches (mm):
Standard Opening Height/Optional
Standard Inflow Velocity
10 (254)/8(203)
105 FPM (.53 m/s)
10 (254)/8(203)/12(305)
105 FPM (.53 m/s)
10 (254)/8(203)/12(305)
105 FPM (.53 m/s)
10 (254)/8(203)/12(305)
105 FPM (.53 m/s)
Work Zone Inches (mm):
Height
Width
Depth measured at 10 inches (254mm)
window height
28 1/2 (724)
34 3/8 (873)
25 3/4 (654)
28 1/2 (724)
46 3/8 (1178)
25 3/4 (654)
28 1/2 (724)
58 3/8 (1483)
25 3/4 (654)
28 1/2 (724)
70 3/8 (1788)
25 3/4 (654)
Viewing Window Inches (mm):
Standard is safety plate sliding glass
Fully closed to
21(533) open
Fully closed to
21(533) open
Fully closed to
21(533) open
Fully closed to
21(533) open
Required Exhaust CFM/CMH (opening)
Standard/Optional:
Canopy Variable Flow Thimble (NU-911)
Canopy Fixed Flow Thimble (NU-907)
10(254) /8(203 )/12(305)
CFM (CMH)
276-501 (649-851)
255-450 (383-765)
341-554 (576-933)
320 (544)
269 (457)
387 (650)
10(254) /8(203) /12(305)
CFM (CMH)
363-588 (617-1000)
295-520 (502-884)
430-655 (731-1113)
426 (724)
359 (610)
483 (821)
10(254) /8(203) /12(305)
CFM (CMH)
451-676 (766-1149)
365-590 (621-1003)
531-760 (909-1291)
531 (902)
445 (756)
610 (1036)
10(254) /8(203) /12(305)
CFM (CMH)
538-763 (915-1297)
436-661 (741-1124)
634-865 (1088-1470)
634 (1077)
532 (904)
763 (1251)
Plant Duct Static Pressure Eng./Metric
0.05-0.1"/1.27-2.54mm
H2O
0.05-0.1"/1.27-2.54mm
H2O
0.05-0.1"/1.27-2.54mm
H2O
0.05-0.1"/1.27-2.54mm
H2O
Heat Rejected, BTU, Per Hour (opening)
(non-vented)
(vented)
10(254) /8(208)
903 / 826
120
10(254) /8(208) /12(305)
1140 / 1020 / 1256
157
10(254) /8(208) /12(305)
1768 / 1611 / 1884
198
10(254) /8(208) /12(305)
1884 / 1768 / 2041
198
Electrical:
Volts, AC 60 Hz
+Amps: Blower/Lights (10/8/12 openings)
Amps: Duplex
Rated Amps:
12 ft. Power Cord (one)
U.L./U.L.-C Listed
115
2.3/2.1
3
10
14 GA - 3 Wire, 15A
U.L./U.L.-C Listed
115
2.9/2.6/3.2
3
14
12 GA - 3 Wire, 20A
U.L./U.L.-C Listed
115
4.5/4.1/4.8
3
16
12 GA - 3 Wire, 20A
U.L./U.L.-C Listed
115
4.8/4.5/5.2
3
16
12 GA - 3 Wire, 20A
Crated Shipping Weight:***
Net Weight
460 lbs. /209 kg.
410 lbs. /186 kg.
530 lbs. /240 kg.
480 lbs. /218 kg.
620 lbs. /281 kg.
570 lbs. /258 kg.
690 lbs. /313 kg.
640 lbs. /290 kg.
***Crated shipping weight does not include weight for accessories or options
+ Based on cabinet with new filters running at 115VAC.

OM0283 Page 20 of 70
Rev 2 July/2021
6.0 Operating the NU-543
6.1 FlowGard™Control System
6.1.1 Overview
The FlowGard™ electronic control system is designed to service the control requirements of the LabGard® ES NU-
543 Biosafety Cabinet. The control system consists of an electronic module that will perform the following
functions:
•Easy user interface via OLED (Organic Light Emitting Diode) display/function keys\
•Language selectable user interface menu’s (English, Spanish, German, French, Chinese, Japanese)
•Control blower via solid state switch.
•Control lights via solid state switch.
•Control outlets via solid state switch.
•Disable audible alarm switch with ring back function.
•Control blower DC ECM motor with solid-state DC Motor Controller that provides automatic
compensation (constant volume control) for both filter loading and line voltage variances.
•Monitor and display airflow velocity performance via Intelliflow Monitor.
•Display time of day and temperature.
The LabGard® ES NU-543 offers the latest digital microprocessor design technology for improved cabinet
performance and safety. The FlowGard™ control system integrates a dual thermistor airflow sensor (Intelliflow) to
monitor the cabinet’s airflow performance. The FlowGard™ control system also integrates a DC motor controller
that provides automatic compensation for both filter loading and line voltage variances. There is additional on/off
control of blower, fluorescent light, ultraviolet light (optional) and outlets. Lastly the control system monitors the
sliding window position with micro switches. All the above functions are shown in a system block diagram (see
figure 1).
Figure 1