Thermo Scientific Dionex UltiMate 3000 Series User manual

Thermo Scientific Dionex
UltiMate 3000 Series
SRD-3x00 Solvent Racks
Operating Instructions
(Original Operating Instructions)
Revision: 1.8
Date: September 2013
© 2013 Thermo Fisher Scientific Inc.
Doc No. 4820.3550

UltiMate 3000 Series:
SRD-3x00 Solvent Racks
Operating Instructions

UltiMate 3000 Series:
SRD-3x00 Solvent Racks
Operating Instructions Page I
Declaration of Conformity
(Original Declaration of Conformity)
Product: Thermo Scientific Dionex UltiMate 3000 Solvent Rack
Types: SR-3000, SRD-3200, SRD-3400, SRD-3600
Dionex Softron GmbH herewith declares conformity of the above products with the
respective requirements of the following regulations:
•Low-Voltage Directive 2006/95/EC
•EMC Directive 2004/108/EC
The electrical safety of the products was evaluated based on the following standard:
•DIN EN 61010-1:2010
Safety requirements for electrical equipment for measurement, control and
laboratory use, Part 1: General Requirements
The Electromagnetic Compatibility (EMC) of the products was evaluated based on the
following standard:
•DIN EN 61326:2006
Electrical equipment for measurement, control and laboratory use
EMC Requirements
This declaration is issued for the manufacturer
Dionex Softron GmbH
Part of Thermo Fisher Scientific Inc.
Dornierstraße 4
D-82110 Germering
by the Managing Director, Rüdiger Obst and
the Vice President HPLC, Fraser McLeod.
September 2, 2013

UltiMate 3000 Series:
SRD-3x00 Solvent Racks
Page II Operating Instructions

UltiMate 3000 Series:
SRD-3x00 Solvent Racks
Operating Instructions Page i
Table of Content
1Introduction................................................................................................................... 1
1.1 How to Use this Manual ............................................................................................ 1
1.2 Safety Information ..................................................................................................... 2
1.2.1 Symbols on the Module and in the Manual...................................................... 2
1.2.2 Safety Precautions ............................................................................................ 3
1.2.3 Consignes de Sécurité.................................................................................. 6
1.3 Intended Use .............................................................................................................. 9
1.4 Federal Communications Commission (FCC) Note................................................ 10
2Overview ...................................................................................................................... 11
2.1 Unit Description....................................................................................................... 11
2.2 SRD Configurations................................................................................................. 11
2.3 Front Panel Elements............................................................................................... 13
2.4 Rear Panel ................................................................................................................ 14
2.5 Fluid Connections.................................................................................................... 15
2.6 Leak Sensor.............................................................................................................. 16
2.7 Wellness................................................................................................................... 16
3Installation................................................................................................................... 17
3.1 Facility Requirements.............................................................................................. 17
3.2 Unpacking................................................................................................................ 17
3.3 Positioning the SRD in the UltiMate 3000 System ................................................. 19
3.4 Connecting the SRD ................................................................................................ 21
3.4.1 Connecting the SRD to an UltiMate 3000 System Pump............................... 21
3.4.2 Connecting the SRD to the Main Power Supply (Optional) ..........................21
4Preparation for Operation (Startup) ........................................................................ 23
4.1 Overview.................................................................................................................. 23
4.2 Connecting Drain Tubing ........................................................................................ 23
4.3 Solvent Reservoirs ................................................................................................... 24
4.3.1 General Notes ................................................................................................. 24
4.3.2 Connecting the Solvent Reservoirs ................................................................ 25
4.3.3 Connecting the Solvent Supply Lines to the Degasser................................... 27
5Operation and Maintenance ...................................................................................... 29
5.1 Choosing the Solvents ............................................................................................. 29
5.2 General Notes for Degasser Operation .................................................................... 31
5.3 Operational Settings................................................................................................. 32
5.3.1 Turning the Degasser on or off (from the UltiMate 3000 System Pump)...... 32
5.3.1.1 Turning the Degasser on and off from the Pump Display....................... 32
5.3.1.2 Turning the Degasser on and off in Chromeleon .................................... 33
5.3.2 Turning the Degasser On or Off (Stand-alone Operation) ............................. 34

UltiMate 3000 Series:
SRD-3x00 Solvent Racks
Page ii Operating Instructions
5.4 Special Chromeleon Functions ................................................................................35
5.4.1 Leak and Vacuum Monitoring........................................................................35
5.4.2 SRD Diagnostics.............................................................................................35
5.5 Shut Down................................................................................................................36
5.6 Routine and Preventive Maintenance ......................................................................37
6Troubleshooting ..........................................................................................................39
6.1 Overview..................................................................................................................39
6.2 Messages on the Pump Display ...............................................................................40
6.3 Chromeleon Diagnostics Message...........................................................................40
6.4 Operating Problems..................................................................................................41
7Service ..........................................................................................................................45
7.1 General Notes and Safety Precautions .....................................................................45
7.2 Rinsing the Degassing Channels..............................................................................46
7.3 Eliminating Leakage in the Solvent Rack................................................................47
8Technical Information ................................................................................................49
9Accessories, Consumables, and Spare Parts.............................................................51
9.1 Standard Accessories ...............................................................................................51
9.2 Optional Accessories................................................................................................53
9.3 Consumables and Spare Parts ..................................................................................53
10 Pin Assignment............................................................................................................55
10.1 15-pin D-Sub Port ................................................................................................55
10.2 DC Input...............................................................................................................55
11 Index.............................................................................................................................57

UltiMate 3000 Series:
SRD-3x00 Solvent Racks
Operating Instructions Page 1
1 Introduction
1.1 How to Use this Manual
The layout of this manual is designed to provide quick reference to the sections of interest
to the reader when operating the Thermo Scientific™ Dionex™ SRD-3x00 Solvent Rack.
However, in order to obtain a full understanding of the Solvent Rack, Thermo Fisher
Scientific recommends that you review the manual thoroughly before beginning operation.
Almost all descriptions in the manual apply to all SRD-3x00 Solvent Racks in the
UltiMate™ 3000 series. Therefore, the term "the SRD" or "the device" is used throughout the
manual. If some detail applies to only one SRD model, the model is identified by name. The
same applies to the descriptions of the Viper™ capillary connections throughout this manual.
They apply also to nanoViper™ capillary connections if not otherwise stated.
Note: The device configuration may vary; therefore, not all descriptions necessarily
apply to your particular instrument.
The representation of a component in this manual may be slightly different
from the real component. However, this does not influence the descriptions.
The software descriptions in this manual refer to Chromeleon™ 6.80 Service
Release 13
This manual is provided "as is." Every effort has been made to supply complete and
accurate information and all technical specifications have been developed with the utmost
care. The information contained in this manual should not be construed as a commitment
by Thermo Fisher Scientific. Thermo Fisher Scientific assumes no responsibility for any
errors that may appear in this document that is believed to be complete and accurate at the
time of publication and, in no event, shall Thermo Fisher Scientific be liable for incidental
or consequential damages in connection with or arising from the use of this document. We
appreciate your help in eliminating any errors that may appear in this document.
The information contained in this document is subject to change without notice.
All rights reserved, including those for photomechanical reproduction and storage on
electronic media. No part of this publication may be copied or distributed, transmitted,
transcribed, stored in a retrieval system, or transmitted into any human or computer language,
in any form or by any means, electronic, mechanical, magnetic, manual, or otherwise, or
disclosed to third parties without the express written permission of Thermo Fisher Scientific
Inc.

UltiMate 3000 Series:
SRD-3x00 Solvent Racks
Page 2Operating Instructions
Trademarks
Analyst is a registered trademark of AB Sciex.
Compass and Hystar are trademarks of Bruker Daltonics.
Empower is a trademark of Waters Corp.
PEEK is a trademark of Victrex PLC.
All other trademarks are property of Thermo Fisher Scientific Inc. and its subsidiaries.
1.2 Safety Information
The CE Mark label and cTUVus Mark safety label on the instrument indicate that the
instrument is compliant with the related standards.
1.2.1 Symbols on the Module and in the Manual
The table shows the symbols used on the instrument:
Symbol Description
Direct current—Courant continu
Refer to the Operating Instructions to prevent risk of harm to the operator and to
protect the instrument against damage.
Référez-vous à ce manuel pour éviter tout risque de blessure à l'opérateur et/ou
protéger l'instrument contre tout dommage
Label according to the "Measures for Administration of the Pollution Control of
Electronic Information Products" (China RoHS) guideline
Étiquette "Measures for Administration of the Pollution Control of Electronic
Information Products" (China RoHS)
WEEE (Waste Electrical and Electronic Equipment) label—For more
information, see the WEEE Information section in the "Installation and
Qualification Documents for Chromatography Instruments" binder.
Étiquette DEEE (Déchets d'Equipements Electriques et Electroniques) — Pour
plus d'informations, référez-vous au chapitre WEEE Information dans le classeur
"Installation and Qualification Documents for Chromatography Instruments".
At various points throughout the manual, the following symbols indicate messages of
particular importance:
Tip: Indicates general information, as well as information intended to
optimize the performance of the instrument.
Important: Indicates that failure to take note of the accompanying information
could cause wrong results or may result in damage to the module.

UltiMate 3000 Series:
SRD-3x00 Solvent Racks
Operating Instructions Page 3
Important: Indique que ne pas tenir compte de l'information jointe peut conduire
à de faux résultat ou endommager l'instrument.
Warning: Indicates that failure to take note of the accompanying information
may result in personal injury.
Avertissement: Indique que ne pas tenir compte de l'information jointe peut entraîner
des blessures corporelles.
1.2.2 Safety Precautions
When working with analytical instrumentation, you must know the potential hazards of
using chemical solvents.
Tip: Before initial operation of the SRD, make sure that you are familiar
with the contents of this manual.
For the safety precautions in French, see section 1.2.3 (→page 6).
Warning: All users of the device must observe the following safety precautions
and all additional safety precautions in this manual to avoid the
possibility of personal injury or damage to the device when
operating the device or carrying out any maintenance or service
procedures.
Observe any warning labels on the device and see the related
sections in these Operating Instructions.
•Protective equipment
When performing any work on or near the HPLC system, wear personal protective
equipment (protective clothing, safety gloves, safety glasses) as required by the hazard of
the mobile phase and sample. For information about the proper handling of a particular
substance and for advice on specific hazards, refer to the material safety data sheet for the
substance you are using. Observe the guidelines of Good Laboratory Practice (GLP).
An eyewash facility and a sink should be close to the device. If any substance splashes on
the eyes or skin, wash the affected area and seek medical attention.
•Hazardous substances
Many organic solvents, mobile phases, and samples are harmful to health. Be sure that
you know the toxic and infectious properties of all substances that you are using. You
may not know the toxic or infectious properties of many substances that you are using. If
you have any doubt about a substance, treat it as if it contains a potentially harmful
substance. For advice on the proper handling of a particular substance, refer to the Safety
Data Sheet (SDS) of the manufacturer. Observe the guidelines of Good Laboratory
Practice (GLP).

UltiMate 3000 Series:
SRD-3x00 Solvent Racks
Page 4Operating Instructions
Dispose of waste substance in an environmentally safe manner that is consistent with all
local regulations. Do not allow flammable, toxic, and/or infectious substances to
accumulate. Follow a regulated, approved waste disposal program. Never dispose of
flammable, toxic, and/or infectious substances through the municipal sewage system.
•Hazardous gases
Install the HPLC system in a well-ventilated laboratory. If the mobile phase or sample
includes volatile or flammable solvents, do not allow them to enter the workspace. If the
mobile phase or sample includes volatile or flammable solvents, avoid open flames and
sparks.
•Electrostatic discharge
Discharge of electrostatic energy may lead to sparking and can constitute a fire hazard.
Keep in mind that liquid flowing through capillaries can generate static electricity. This
effect is particularly pronounced in insulating capillaries and with non-conductive
solvents (for example, pure acetonitrile).
Take appropriate measures to prevent the generation of static electricity near the HPLC
system. For example, make sure that the air humidity level in the laboratory is
sufficiently high and provide proper ventilation, wear anti-static clothing or shoes,
prevent accumulation of air bubbles in waste lines, and use grounded waste containers.
Use only non-conductive capillaries to direct solvents into the waste container. With
electrically conductive capillaries, make sure that they are properly grounded.
•Self-ignition of solvents
Do not use solvents for which the self-ignition temperature is below 150 °C. In case of
leakage, these solvents may self-ignite on a hot surface.
•Capillaries, capillary connections, open connections
♦Capillaries, especially non-metallic capillaries may burst, slip out of their fittings or
may not be screwed in. This may result in substances spraying out of the open
connections.
♦In an UltiMate 3000 system, some components are made of PEEK™. This polymer
has superb chemical resistance to most organic solvents. However, it tends to swell
when in contact with trichlormethane (CHCl3), dimethyl sulfoxide (DMSO), or
tetrahydrofuran (THF). In addition, it is attacked by concentrated acids, such as,
sulfuric acid and nitric acid or a mixture of hexane, ethyl acetate, and methanol. In
both cases, capillaries may start leaking or they can burst. Swelling or attack by
concentrated acids is not a problem with brief flushing procedures.
♦Do not use tubing that is stressed, bent, kinked, or damaged.
♦Capillary connections can be contaminated by harmful substances or harmful
substances can escape from open connections.