Agilent Technologies NovoCyte Quanteon Manual

NovoCyte Quanteon Flow Cytometer
Operator's Guide
For Research Use Only. Not for use in diagnostic procedures.

Notices
Manual Part Number
150135
Third Edition, March 2021
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In This Guide
NovoCyte Quanteon Flow Cytometer Operator's Guide 3
In This Guide
This guide covers following module: NovoCyte Quanteon Flow Cytometer and
associated standard accessories.
1Prologue
This chapter describes the purpose and scope of this guide, symbols used in this
guide, glossary, safety and limitations, Agilent regulatory compliance statement
and technical support information.
2Introduction
This chapter provides overview of NovoCyte Quanteon flow cytometer, including
basic instrument operation principle, system requirements, and system
components.
3Installation
This chapter provides information on how to install NovoCyte Quanteon
instrument and associated standard accessories, including installation warnings,
space and environment requirements, instructions for lifting and carrying, and
installation procedures.
4Instrument Operation
This chapter provides the procedures to operate NovoCyte Quanteon flow
cytometer, including instrument startup, verify and modify instrument
configuration, instrument QC test, and instrument shutdown.
5Running Samples
This chapter describes the typical procedures of running examples on NovoCyte
Quanteon instrument using human peripheral blood lymphocyte subsets analysis
as an example.
6System Maintenance
This chapter describes the procedures to maintain NovoCyte Quanteon flow
cytometer, including scheduled and unscheduled maintenance.
7Troubleshooting

In This Guide
4 NovoCyte Quanteon Flow Cytometer Operator's Guide
This chapter describes general instrument troubleshooting and diagnostic
guidelines.
8Version History
Appendix A: NovoCyte Quanteon Laser and Detection Channel
Configuration
This chapter provides additional information on NovoCyte Quanteon laser and
detection channel configuration.
Appendix B: NovoCyte Quanteon Technical Specifications
This chapter provides technical specifications of NovoCyte Quanteon flow
cytometer and associated standard accessories.

Contents
NovoCyte Quanteon Flow Cytometer Operator's Guide 5
Contents
In This Guide 3
Contents 5
1Prologue 11
Using this Guide 11
Symbols 11
Glossary 14
Safety and Limitations 16
General Safety 17
Ergonomics 19
Electrical Safety 20
Laser Safety 20
Laser Product Classification 21
Biosafety 21
Limitations 22
Agilent Regulatory Compliance Statement 23
CE Compliance 23
Electromagnetic Compatibility 23
EMC Declaration for South Korea 25
Detachable Power Cord Declaration for Japan 25
Sound Emission Certification for Federal Republic of Germany 25
Waste Electrical and Electronic Equipment (WEEE) Directive 25
Regulatory Compliance Identification Number 26
Technical Support 26
2Introduction 29

Contents
6 NovoCyte Quanteon Flow Cytometer Operator's Guide
Overview 29
Basic Principle 29
Optics System 29
Fluidics System 37
Electronics System 41
System Requirements 43
Software 43
Workstation 43
Fluidics Station 43
Compatible Sample Tubes 44
Instrument Reagents 44
QC Particles 44
System Components 44
NovoCyte Quanteon Instrument 45
NovoCyte Workstation and Monitor 46
NovoCyte Fluidics Station II 47
NovoCyte Fluidics Cart (Optional) 49
NovoSampler Q (Optional) 49
3Installation 51
Installation Warnings 51
Space Requirements 52
Environmental Requirements 52
Instruction for Lifting and Carrying 52
Install NovoCyte Quanteon 53
Connect NovoCyte Workstation 54
Connect NovoCyte Fluidics Station II 54

Contents
NovoCyte Quanteon Flow Cytometer Operator's Guide 7
Connect NovoCyte Fluidics Cart (Optional) 55
Connect NovoSampler Q (Optional) 55
Connect Instrument to Power Supply 56
4Instrument Operation 57
Instrument Start-up 57
Pre-Inspection 57
Power on the Instrument 57
Turn on Workstation 58
Add Instrument Reagents 59
Empty Waste 60
Verify and Modify Instrument Configuration 61
View and Verify Instrument Configuration 61
Modify Instrument Configuration 61
Instrument QC Test 68
Instrument Shutdown 68
5Running Samples 71
Overview 71
Create Experiment 72
Define Sample Acquisition Parameters 74
Set Sample Acquisition Conditions 76
Stop Condition 76
Flow Rate 77
Threshold 77
Run Sample 80
Perform Fluorescence Compensation 82
Automatic Fluorescence Compensation 82

Contents
8 NovoCyte Quanteon Flow Cytometer Operator's Guide
Manual Fluorescence Compensation 92
Analyze Data 97
Append Data 101
Delete Data 102
Save Data 102
Import and Export Files 103
6System Maintenance 107
Scheduled Maintenance 107
Preventative Maintenance 108
Replacing Fluidic System Consumables 113
Replace 0.05 µm Sheath Fluid Ultrafiltration (UF) Filter (Optional) 122
Unscheduled Maintenance 125
Debubble 125
Clean the External Surface of the Instrument 126
Decontamination 126
Prime Fluidic System before Long-Term Storage 127
Prime Fluidic System after Long-Term Storage 128
Purge Fluidic System before Shipment 128
Calibrate NovoCyte Fluidics Station II 130
Clean the Optical Filters 131
Clean Fluidic System 132
7Troubleshooting 133
8Version History 137
Appendix A: NovoCyte Quanteon Laser and Detection Channel
Configuration 139
Laser Configuration 139

Contents
NovoCyte Quanteon Flow Cytometer Operator's Guide 9
Detection Channel Configuration 139
Appendix B: NovoCyte Quanteon Technical Specifications 143
NovoCyte Quanteon Technical Specifications 143
NovoCyte Workstation and Monitor Specifications 146
NovoCyte Fluidics Station II Specifications 146

Contents
NovoCyte Quanteon Flow Cytometer Operator's Guide 10
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