Sper scientific 300003 User manual

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SUGAR/BRIX
REFRACTOMETER
300003
INSTRUCTION MANUAL

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TABLE OF CONTENTS
INTRODUCTION .............................................................3
PANEL DESCRIPTION....................................................3
OPERATING PROCEDURES......................................4-5
TEMPERATURE CORRECTION....................................5
TEMPERATURE CORRECTION TABLE .......................6
PRECAUTIONS ...............................................................7
SPECIFICATIONS ...........................................................7
WARRANTY .....................................................................8

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INTRODUCTION
Your portable refractometer is a precision optical instrument
designed to measure the concentration of sugar in aqueous
solutions. It utilized the standardized Brix scale which is
accurate and easy to read. The unit’s lightweight, ergonomic
design makes it convenient for both laboratory and eld
applications. It is excellent for quality assurance, process
control requirements, and scientic research.
The refractometer operates on the principle that as the
concentration or density of a solution increases, its refractive
index changes proportionately. The refractive angle
measured by your refractometer registers on the scale. The
larger the concentration of sugar in solution, the higher the
reading on the scale.
PANEL DESCRIPTION:
Cover Plate (1)
Light reecting window (2)
Refracting prism (3)
Calibration screw (4)
Telescopic barrel (5)
Scale selector (6)
Eyepiece with focus ring (7)

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OPERATING PROCEDURES
1. With the COVER PLATE (1) open, carefully clean the
PRISM (3) and REFLECTING WINDOW (2) with a soft
cloth. Avoid scratching the surfaces.
2. Aim the refractometer toward a light source and select
the 0-50% scale by rotating the SCALE SELECTOR (6).
3. Rotate the EYEPIECE (7) to obtain the clearest focus.
4. Adjustment of the null (reference point):
A. Open the COVER PLATE (1).
B. Apply a few drops of pure distilled water onto the
REFRACTING PRISM (3).
C. Close the COVER PLATE (1).
D. Turn the CALIBRATION SCREW (4) so that the dark
and light boundary line coincides exactly with the 0%
line reading on the scale in the 0-50% range.
5. Carefully dry the prism platform and the LIGHT
REFLECTING WINDOW (2) with a soft cloth.
6. To test your sugar sample, rotate the SCALE SELECTOR
(6) to select the 0-50% range or the 50-80% range.
7. Place a few drops of the test solution on the prism and
close the COVER PLATE (1) so the solution spreads
evenly on the prism.
8. Aim the light reecting windows toward the light source
and focus the eyepiece on the boundary line of the light
and dark hemispheres.
9. The boundary line indicates the concentration of sugar in
the test sample.
10. If the boundary line is too low or too high to be read on
the scale you selected, rotate the SCALE SELECTOR
(6) to the other scale.
11. After use, clean the refracting and illuminating prisms
with a soft cloth to remove any surface residue.
12. The temperature of the null reference liquid should be
at the same temperature as the sample solution. For
variations in temperature during use, the null point
should be recalibrated once every 30 minutes.

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TEMPERATURE CORRECTION
Your refractometer is designed to operate with distilled
water and sample solutions at a standard temperature of
20°C (68°F). To compensate for temperature conditions
other than 20°C, use the Temperature Correction Table on
Page 6.
Add to the reading when the temperature is above
20°C. Example: Actual temperature is 30°C. The
boundary line reads a concentration of 15%. Refer to
the Temperature Correction Table and locate the 15%
concentration column. Follow this column down to the row
that corresponds to the temperature of 30°C. At the 0.78
correction point, the actual concentration percent is
15% + 0.78%, or 15.78%.
Subtract from the reading when the temperature is
below 20°C Example: Actual temperature is 16°C. The
boundary line reads a concentration of 40%. Refer to
the Temperature Correction Table and locate the 40%
concentration column. Follow this column down to the row
that corresponds to the temperature of 16°C. At the 0.30
correction point, the actual concentration percent is
40% - 0.30%, or 39.70%.
If the null liquid (distilled water) is the same temperature as
the test sample and the null line of distilled water reads 0%,
it is not necessary to refer to the correction table.
0-50% scale
-50
-45
-40
-35
-30
-25
-15
-10
-5
-0
50-80% scale
85—
80—
75—
70—
65—
60—
55—
50—

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PRECAUTIONS
With reasonable care, your refractometer’s reliability,
precision and optical performance will not change.
● Never submerge the unit, and do not let liquid to
seep into the unit’s body.
● Clean the refractometer after each use with a soft
cotton cloth. Do not scratch surface of the prisms.
● Store in a dry, clean, and non-corrosive environment.
● Avoid strong shocks.
SPECIFICATIONS
Measuring
Range
0-80%
Resolution 1%
Accuracy ±1%
Size 7x 1 1/2”
Weight 19 oz
Accessories Screwdriver, Carrying Case, Transfer
Pipette, Distilled Water, Instruction Manual.

8Rev 8/1/2016
WARRANTY
Sper Scientic warrants this product against defects in
materials and workmanship for a period of ve (5) year from
the date of purchase, and agrees to repair or replace any
defective unit without charge. If your model has since been
discontinued, an equivalent Sper Scientic product will be
substituted if available. This warranty does not cover probes,
batteries, battery leakage, or damage resulting from accident,
tampering, misuse, or abuse of the product. Opening the
meter to expose its electronics will break the waterproof seal
and void the warranty. To obtain warranty service, ship the
unit postage prepaid to:
SPER SCIENTIFIC LTD.
8281 E. Evans Rd., Suite #103
Scottsdale, AZ 85260
[phone]
The defective unit must be accompanied by a description of
the problem and your return address. Register your product
online at www.sperwarranty.com within 10 days of purchase.