Sper scientific 850048 User manual

DO Meter Kit
850048
Instruction Manual

2
FIRST TIME USE or USE AFTER BEING UNPLUGGED
FOR MORE THAN ONE HOUR.
This instrument requires calibration prior to operating.
After attaching the probe for the rst time, please allow
the probe to warm up for 30 MINUTES before beginning
calibration. Please see calibration instructions on page 9.
When using the meter, press the buttons rmly for
one full second to ensure proper operation.
DO Meter Kit 850048
Copyright ©2013 by Sper Scientic
ALL RIGHTS RESERVED
Printed in the USA
The contents of this manual may not be reproduced
or transmitted in any form or by any means electronic,
mechanical, or other means that do not yet exist or
may be developed, including photocopying, recording,
or any information storage and retrieval system
without the express permission from Sper Scientic.

3
TABLE OF CONTENTS
Introduction. . . . . . . . . . . . . . . . . . . . . . . 4
Attaching and Detaching the Probe . . . . . . . . . . 5
Front Panel Description . . . . . . . . . . . . . . . . 6
LCD Display Description . . . . . . . . . . . . . . . . 7
Dissolved Oxygen % Saturation Calibration . . . . . . 8
Measurement Procedures . . . . . . . . . . . . . . . 9
Function Settings. . . . . . . . . . . . . . . . . . . .11
Memory Clear (P10) . . . . . . . . . . . . . . . . . 12
DO Review and Set Parameters (P60) . . . . . . .13
Select Temperature Units (P70) . . . . . . . . . . .14
Restore Default Settings (P90) . . . . . . . . . . .15
pH Electrode Slope (P20) . . . . . . . . . . . . . .16
Conductivity Calibration (P30) . . . . . . . . . . . .17
Conductivity Cell Constant (P40) . . . . . . . . . .18
DO Probe Maintenance . . . . . . . . . . . . . . . .19
Battery Installation and Replacement . . . . . . . . .21
Troubleshooting . . . . . . . . . . . . . . . . . . . . 21
Error Codes . . . . . . . . . . . . . . . . . . . . . .22
Specications . . . . . . . . . . . . . . . . . . . . .23
Appendix A: pH Probe (Optional) . . . . . . . . . . .25
Appendix B: Conductivity/Salinity Probe (Optional) . .29
Warranty . . . . . . . . . . . . . . . . . . . . . . . . 36

4
INTRODUCTION
This meter reads dissolved oxygen (DO) for measuring
water quality in labs, industrial and municipal waste water,
aquariums, sh hatcheries and environmental testing.
Features include automatic temperature compensation
(ATC) and manual altitude and salinity compensation.
The meter also provides readings in either °C or °F,
multiple calibration points (for optional pH and
conductivity probes), 99 memory points, a large backlit
LCD screen, a low battery icon and one-hour automatic
shut off. A Set Up Mode allows you to customize the
meter’s defaults and parameters.
The meter comes complete with DO probe, replacement
electrolyte uid, 2 replacement membrane sets, carrying
case, batteries, and instruction manual.
Optional Accessories:
• Replacement DO Probe - 850048DO
(includes 2 probe heads & electrolyte)
• Conductivity/Salinity Probe - 850048C/S
• pH Probe - 850048PH

5
ATTACHING AND DETACHING THE PROBE
Always turn the power OFF before attaching and
detaching the probe, and use caution. Please use
alignment quide markings on probe and meter for
proper connection (see image below).
To attach the probe to the meter:
1. Plug the probe into the connector port on top of
the meter.
2. Screw down the attached probe guard until it is
moderately tight.
To detach the probe from the meter:
1. Unscrew the probe guard (the piece that is directly on
top of the meter that connects the probe to the meter).
2. Holding the plug, pull the probe away from the meter.
DO NOT hold the cable and pull the probe and meter
apart as this may damage the probe connection.
Marker on
Meter Connection
Marker on
Probe Connection

6
FRONT PANEL DESCRIPTION
POWER/SET - Press to turn the meter on
and off. In Normal Mode, press for >2 seconds
to enter Function (Set) Mode.
CAL/ESC - Press to switch between Normal
and Calibration Modes. Press to return to
Normal Mode from Function or Recall Modes.
EC/SALT - If using with the optional conductivity
probe, press to switch between CON (µs) or
Salt (ppt) units.
RECALL - Press for >2 seconds to read the
memory value.
% / mg/L - Press to switch between
DO in % and mg/l.
▲- Press to increase the displayed value
in Function or Calibration Modes.
MEM - Press to save the current reading.
▼- Press to decrease the displayed value in
Function or Calibration Modes.
ENTER - Press to conrm the calibration or
parameter setting.
SET
ESC
CAL
EC/SALT
RECALL
% / mg/L
MEM
ENTER
SET
ESC
CAL
EC/SALT
RECALL
% / mg/L
MEM
ENTER
SET
ESC
CAL
EC/SALT
RECALL
% / mg/L
MEM
ENTER
SET
ESC
CAL
EC/SALT
RECALL
% / mg/L
MEM
ENTER
SET
ESC
CAL
EC/SALT
RECALL
% / mg/L
MEM
ENTER
SET
ESC
CAL
EC/SALT
RECALL
% / mg/L
MEM
ENTER

7
LCD DISPLAY DESCRIPTION
• The DO value is displayed
on the left side of the LCD.
• The temperature value is
displayed on the upper
right side of the LCD.
• The total number of records saved is displayed on the
lower right side of the LCD.
O
C
TEMP
MEMO
mg/l
O
ATC
7.The DO value will be displayed on the
left side of the LCD.
8.The temperature value will be
displayed in the upper right corner of
the LCD.
9.The MEMO number which is the total
number of records saved in the meter
will be displayed in the lower right
corner of the LCD.
4
DD
D.O. probe
DO PROBE (please inspect upon receiving)
Clean the electrode (see pg. 19) if there is too much white
residue attached to it. It is normal to see the cloudy/milky
electrolyte and there is no need to replace it for this
reason. The best condition of the electrode is a clean
surface. However, some white residue attached on
electrode is also ne.

8
DISSOLVED OXYGEN % SATURATION CALIBRATION
After attaching the probe, please allow the probe to
warm up for 30 minutes before beginning calibration.
Calibrate the meter before each use.
1. Remove the protective cap from the DO probe.
2. Press the power button to turn the meter ON.
See p.16 to adjust salt & altitiude value if necessary.
3. Hold the probe in a well ventilated area for several
minutes (it may take 5-10 minutes) until the LCD
reading has stabilized. This is particularly important
if you have just relled the electrolyte solution or
replaced either the batteries or the membrane set.
4. Press CAL/ESC for 2 seconds to calibrate for 100%
saturation. The CAL icon will ash on the LCD.
5. Press ENTER to nish the
calibration.
6. If “ERR” appears during
calibration, there is an error
due to low electrolytes or a
defective probe.
Note…
To exit Calibration Mode without saving the new value,
press CAL/ESC. The meter will retain the previously saved
calibration data for the current range.
O
C
DO
%
CAL
22
8.Repeat step 1~8 for other ranges
calibration if needed.
NOTE:
If you would like to exit the conductivity
calibration mode without saving,
please press CAL/ESC key instead
and it will retain the meter's previous
calibration data for the current range.
We recommend the users to calibrate
the meter before operate it each time.
Calibration Step for DO % SATURATION
a.In normal mode, hold the probe in
the air , wait for few minutes until the
reading on LCD is stabilized. Press
CAL/ESC to calibrate for 100%
saturation calibration, CAL icon is
flashing on LCD.(Fig 23)
b.Wait couple seconds, when the
reading is stable, press ENTER to
finish the calibration.
c.You can stop the calibration by
pressing CAL/ESC.
d.Whenever an error occurs during
calibrating, the ERR indicator will
appear. It might cause by low
electrolyte condition or probe defect.
Fig. 23
DO % SATURATION CALIBRATION
Note:
While following condition happens,
Please turn on the meter and wait for

9
MEASUREMENT PROCEDURES
1. Remove the protective cap from the DO probe.
2. Insert the DO probe into the meter.
3. Press the power button to turn the meter ON.
4. Hold the probe in the air until the LCD reading has
stabilized. This may take several minutes.
5. The DO reading will be displayed on the LCD.
You may change the display from % to mg/L
as desired by pressing % / mg/L.
6. Place the probe into the sample solution, making
sure that the electrode is completely immersed in
the sample.
7. Stir the probe gently to create a homogenous sample.
8. Wait until the reading has stabilized.
Storing DO Readings in Memory
1. To store the reading in memory, the value must be
displayed in mg/l. Press % / mg/L if needed to change
from % to mg/L.
2. Press the MEM button to save the value.
3. The memory number will be displayed on the LCD.
If the memory is full, “FUL” will be displayed. See
Memory Clear Function on p.12.

10
Recall Readings
1. Press and hold RECALL for 2 seconds to enter
Recall Mode.
2. Press ▲ or ▼ to scroll through the data in memory.
3. Press CAL/ESC for 2 seconds to return to
Normal Mode.
O
C
DO
TEMP
MEMO
mg/l
ATC
This meter has the advanced setup
mode which allows you to customize
and check your meter's preferences
and defaults.If you would like to
change the parameters, please press
" SET " for more than 2 sec. to enter
the setup mode when the meter is in
the measurement mode.
NOTE:
To exit the function without saving in
the setup mode, press the CAL/ESC
key until the measurement mode
appears. If the meter is under the
setting value status, press the
CAL/ESC key twice to exit.
SETUP
9
Fig 18
Press any key to actirate the
backlight for 10 seconds
BACKLIGHT