STEMCELL RoboSep-16 Assembly instructions

1
USER REFERENCE MANUAL
RoboSep™-16

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Table of Contents
Overview of Safety Symbols ...................................................................................................iv
Safety Information and Warnings ...........................................................................................iv
Safety Precautions....................................................................................................................v
Operation ..................................................................................................................................................v
Maintenance............................................................................................................................................. vi
Computer ................................................................................................................................................ vii
Software .................................................................................................................................................. vii
1.0 Introduction ...................................................................................................................1
1.1 RoboSep™-16 System Overview .................................................................................................... 2
1.2 Liquid Pipetting Robot ...................................................................................................................... 3
1.3 The Deck .......................................................................................................................................... 3
1.3.1 Buffer Solution Bottle Holders .................................................................................................. 4
1.3.2 Slide-Out Tray .......................................................................................................................... 4
1.3.3 Disposable Tip Racks .............................................................................................................. 6
1.3.4 Waste Bin ................................................................................................................................. 6
1.3.5 Reagent Vial Rack.................................................................................................................... 7
1.4 Barcode Scanner ............................................................................................................................. 8
1.5 Computer Controller and RoboSep™-16 Application ...................................................................... 8
1.6 User Interface Overview................................................................................................................... 8
1.6.1 Screens .................................................................................................................................... 8
1.6.2 Icons....................................................................................................................................... 10
1.7 Clean Air Protection (CAP) System Overview ............................................................................... 11
2.0 Installation ...................................................................................................................12
2.1 Placement of the Instrument .......................................................................................................... 12
2.2 Power and Network Connections................................................................................................... 12
2.2.1 Configuring the Computer to Access RoboSep™-16 ............................................................ 13
2.2.2 Configuring the Computer to Access the Barcode Scanner .................................................. 14
2.3Startup............................................................................................................................................ 15
2.4 Shutdown ....................................................................................................................................... 15
3.0 User Profiles ................................................................................................................16
3.1 Adding a User Profile ..................................................................................................................... 16

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3.2 Deleting a User Profile....................................................................................................................17
3.3 Editing a User Profile ......................................................................................................................17
4.0 User Preferences.........................................................................................................17
4.1 Hardware and Software Preferences .............................................................................................17
4.2 Administrator Settings.....................................................................................................................19
4.2.1 Admin Mode............................................................................................................................19
4.2.2 Change Admin Password ....................................................................................................... 19
5.0 RoboSep™-16 Operation ............................................................................................20
5.1 RoboSep™ Protocols .....................................................................................................................20
5.1.1 Adding New Protocols to RoboSep™-16 ............................................................................... 20
5.1.2 Deleting Protocols from RoboSep™-16 ................................................................................. 20
5.2 Setting Up a Run ............................................................................................................................ 20
5.2.1 Selecting a Cell Separation Protocol ......................................................................................20
5.2.2 Protocol Presets .....................................................................................................................22
5.2.3 Preparing the Cells ................................................................................................................. 23
5.2.4 Loading the Deck and Starting a Run.....................................................................................23
5.2.5 Collecting Isolated Cells .........................................................................................................26
6.0 CAP System Operation ...............................................................................................27
6.1 Turning on the CAP System ...........................................................................................................27
6.2 User Interface ................................................................................................................................. 28
6.3 System Configuration Options ........................................................................................................28
6.3.1 Schedule .................................................................................................................................29
6.3.2 Filter Change ..........................................................................................................................30
6.3.3 System .................................................................................................................................... 30
6.3.4 Alerts.......................................................................................................................................30
6.4 Replacing Filters ............................................................................................................................. 30
6.4.1 Replacing Pre-Filters ..............................................................................................................30
6.4.2 Replacing HEPA Filters .......................................................................................................... 31
7.0 End of Run Report Files..............................................................................................33
8.0 Care and Maintenance ................................................................................................34
8.1 Maintenance ...................................................................................................................................34
8.2 Replacing the Fuses .......................................................................................................................36

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9.0 Specifications ..............................................................................................................37
9.1 RoboSepTM-16................................................................................................................................ 37
9.2 CAP System ................................................................................................................................... 38
10.0 Troubleshooting ..........................................................................................................39
10.1 Service Logs................................................................................................................................... 39
10.2 Diagnostic Package ....................................................................................................................... 39
11.0 Software Updates ........................................................................................................40
12.0 Related Products .........................................................................................................40

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Overview of Safety Symbols
The BIOHAZARD symbol indicates that special care is required to remain
protected from biological hazard.
The WARNING symbol indicates where the operator must follow
instructions to avoid personal injury or damage to the instrument.
Safety Information and Warnings
STEMCELL Technologies is not responsible for any injury or damage caused by the use of this instrument for
purposes other than those for which it is intended, or by modifications of the instrument or software not
performed by an authorized STEMCELL Technologies representative.
RoboSep™-16 must be operated by trained professionals and is intended
for research use only.
RoboSep™-16 should be placed on a clean, flat surface free of any foreign
objects or debris and insulated from excessive vibration. Provide adequate
space in front of and above the system for opening the door and working
with the instrument and reagents. Provide adequate space nearby to place
the laptop and optional handheld barcode scanner.
Always connect the power supply to a 3-prong, grounded AC outlet rated
5A 100 - 240V using the AC power cord provided with RoboSep™-16. Do
not use an adapter to a two-terminal outlet. Before plugging the instrument
in, be sure that two T5AL 250V~ fuses are installed.
All electrical devices pose a risk of electric shock. To reduce this risk, do
not open any covers that are fastened with screws. While RoboSep™-16 is
designed to withstand spills, do not allow fluids to enter the interior of the
instrument. In the event of such a spill, switch off RoboSep™-16 and
disconnect the power cable before cleaning (section 8.0).
Operate RoboSep™-16 at 15 to 30°C, in a non-condensing environment
with a relative humidity of 30 to 85% and at an altitude of 0 to 2000 m
above sea level. RoboSep™-16 should be used indoors only.
RoboSep™-16 is not specified for use inside an incubator or cold room.
Protect the instrument against dust and moisture.

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EasySep™ magnets on the RoboSep™-16 deck generate strong magnetic
fields. Keep away from pacemakers, watches, or other objects that respond
to magnetic fields.
RoboSep™-16 weighs 122 kg (270 lb). Never move a fully assembled
instrument by yourself. Use the moving straps attached to each leg of the
instrument for moving. At least 4 people are required to move the
instrument. Always remove labware and carriers prior to moving.
Proper installation and service of the instrument by an authorized
STEMCELL representative is required to provide assurance that the
instrument performs within the acceptable limits for its intended use.
In case of malfunction, call STEMCELL Technologies Inc. for service.
Servicing performed by anyone other than an authorized STEMCELL
Technologies representative will void the warranty associated with
RoboSep™-16. There are no user-serviceable parts inside the instrument
and the outer casing should never be removed.
Safety Precautions
Operation
When using RoboSep™-16, good laboratory practices (GLP) must be
observed. Suitable protective clothing, safety glasses and protective gloves
must be worn.
If working with biohazardous samples, observe and carry out the
maintenance procedures, paying particular attention to cleaning and
decontamination. Wear gloves when handling all parts of the instrument.
Avoid direct contact with tips discarded into the waste bin. Any surfaces on
which liquid is spilled must be decontaminated.
Ensure the instrument is fully opened and secured before accessing the
contents on the deck of the instrument. This will prevent accidental closure
of the door.
Waste may contain biohazardous or chemical-contaminated material.

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Empty the RoboSep™-16 waste bin (section 1.3.4) only after a run is
complete or the instrument is paused. Before initiating a run, ensure there
is adequate space remaining in the waste bin to hold tips that will be used
during operation.
Do not overfill reagent containers, tubes, or other liquid containers.
Promptly clean any spills around the instrument. Clean any spills in
RoboSep™-16 only after a run is complete or the instrument is paused.
Do not remove the instrument side covers under any circumstances.
Always have the door closed when executing runs. During operation, the
instrument arm may move suddenly causing bodily harm.
Contact STEMCELL Technologies Technical Support anytime you believe the instrument has lost accuracy or
precision in any motion axis.
Maintenance
If RoboSep™-16 becomes contaminated with biohazardous or chemical
material, clean it as described in section 8.0.
Observe and carry out the maintenance procedures given in section 8.0.
Failure to do so may impair the reliability and correct functioning of
RoboSep™-16.
Preventive maintenance beyond daily and weekly maintenance, cleaning,
and decontamination must only be performed by an authorized STEMCELL
representative.
Do not use an isopropyl alcohol-based solution to clean the door.

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Computer
Take the necessary precautions to guard against software viruses.
Running other software in parallel to the RoboSep™-16 application may
negatively affect the operation of the instrument.
Any manipulation of RoboSep™-16 application files can result in erroneous
test results or instrument failure.
Only the RoboSep™-16 application may be used to control RoboSep™-16.
For reasons of data security and integrity, use of an uninterrupted power
supply (UPS) is recommended, since a loss of power may cause data to be
lost or corrupted.
Software
This manual is applicable to RoboSep™-16 software version 1.2.2.0. For other software versions, contact us
at [e-mail] for the applicable manual.

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1.0 Introduction
RoboSep™-16 is a fully automated cell separation instrument that seamlessly integrates multi-sample cell
isolation into any laboratory environment. Using EasySep™, the column-free immunomagnetic cell separation
system, RoboSep™-16 isolates desired cells from a wide range of samples from different species, tissue
sources or sizes using either positive or negative selection. Through an intuitive user interface,
RoboSep™-16 can be instructed to simultaneously isolate cells from up to 16 samples with minimal hands on
time. By automating all reagent and sample handling steps, and through the use of disposable pipette tips,
RoboSep™-16 rapidly isolates all desired cells while minimizing the risk of sample cross-contamination.
After completion of the cell isolation procedure, cells of interest are immediately available for any downstream
application.

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1.1 RoboSep™-16 System Overview
Figure 1. Front View of RoboSep™-16 with the Door Closed
RoboSep™-16 is a fully automated cell separation instrument utilizing the column-free immunomagnetic
EasySep™ system.
The major components of the RoboSep™-16 system are:
•Liquid pipetting robot (gantry) (section 1.2 )
•The deck (section 1.3)
•Barcode scanner (section 1.4)
•Computer controller (section 1.5)
With these systems acting in concert, RoboSep™-16 performs the following actions to automate EasySep™
cell separation:
•Accurately dispenses magnetic labeling reagents into cell samples using disposable 1 mL tips
•Mixes and transfers the sample between tubes using disposable tips
•Transfers buffer solution to sample tubes using disposable tips
•Tracks usage of disposable tips
•Scans and records reagent catalog and lot numbers
•Uses an alternate set of reagents if insufficient amounts are detected in the primary set

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RoboSep™-16 automatically executes all actions required to process up to 16 samples, with the flexibility to
perform different types of separations simultaneously.
The indicator lights in the front indicate the instrument status as shown in Table 1.
Table 1. Indicator Lights and Instrument Status
INDICATOR LIGHTS INSTRUMENT STATUS
Blinking Instrument in error state
Pulsing Gantry in motion
Slowly pulsing Gantry is idle
1.2 Liquid Pipetting Robot
The four pipetting channels of the robot are mounted within the gantry arm (Figure 1), which moves all four
channels simultaneously across the deck. The channels have independent spacing between each other, such
that they can independently operate on four vials in parallel. The four channels will pick up every other tip in a
row of the 96-tip rack. Each channel can aspirate, mix, or dispense up to 1 mL. At the end of each liquid
transfer, the tips are disposed in a waste bin to avoid cross-contamination between samples.
1.3 The Deck
The deck components are shown in Figure 2.
Figure 2. The Deck

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To open the RoboSep™-16 door and access the deck:
1. Grasp the handle at the front of the RoboSep™-16 door (Figure 1).
2. Pull the door outward.
3. Move the door upward until it snaps into place. The deck will now be accessible.
The RoboSep™-16 door must be latched in the upper position to prevent
accidental closure while accessing the deck.
To close the RoboSep™-16 door:
1. Grasp the handle at the front of the RoboSep™-16 door (Figure 1).
2. Pull the door outwards.
3. Move the door downwards and push it back into place until fully closed.
The RoboSep™-16 door must be closed during the run.
1.3.1 Buffer Solution Bottle Holders
Up to six buffer solution bottles can be placed on the deck (Figure 2). Buffer bottles are used in sets of two to
accommodate the simultaneous operation of all four pipetting channels.
For each run, two bottles should be filled with RoboSep™ buffer and placed in positions 1 and 2 of the buffer
solution bottle holder. The holder can accommodate four spare bottles (positions 3, 4, 5, and 6) for use with
custom protocols. Contact STEMCELL Technologies Technical Support for additional information.
1.3.2 Slide-Out Tray
The slide-out tray (Figure 3) can accommodate the following:
•16 EasySep™ Magnets (each containing a 5 mL tube)
•Magnet shield
•Sample tube rack (containing up to 16 x 5 mL sample tubes)
•Negative fraction rack (containing up to 16 x 14 mL tubes)
Note: The magnet shield must be placed between the EasySep™ Magnets and the racks (Figure 3). Falcon®
Round-Bottom Polystyrene Tubes are recommended (5 mL [Catalog #38007] and 14 mL [Catalog #38008]).

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Figure 3. Slide-Out Tray
The sample tubes, magnets and negative fraction tubes are all arranged in the same 4 x 4 configuration to
facilitate locating the tubes corresponding to a particular sample and protocol. An alpha-numeric scheme is
used for the identification of each of the 4 x 4 locations. The numbers 1 - 4 refer to the individual protocol
groups whereas the letters A - D refer to the sample used within each group. Thus, for protocol group 1, the
sample tubes, magnets, and negative fraction tubes used will correspond to positions A1, B1, C1 and D1 in
the deck. Up to 4 different protocols can be operated at once, and up to 4 different sample volumes can be
set for each protocol. Refer to section 5.2 for additional information on setting up a run.
For ease of loading and unloading, the sample tube rack and negative fraction rack can be removed from the
slide-out tray and placed onto the bench. Locating pins under the racks facilitate their correct placement and
orientation when loading onto the slide-out tray.
To slide out the tray (Figure 4):
1. Remove the negative fraction rack.
2. Push down on the handle at the front of the tray.
3. Pull the tray outwards.
Note: The tray can only slide out if the negative fraction rack has been removed.
To slide the tray back into place:
1. Push the tray towards the back of the instrument.
2. Push down on the handle at the front of the tray.
3. While holding down the handle, continue pushing the tray until it is locked back into place.
4. Release the handle.
Note: The negative fraction rack should only be placed back onto the deck when the tray is fully stowed in
place.

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Figure 4. Sliding Out the Tray. (A) Tray loaded with racks and magnets. (B) Negative fraction rack removed.
(C) After sliding out the tray.
1.3.3 Disposable Tip Racks
Filtered Conductive 1 mL Tips (Catalog # 23101 or 23102) must be used.
Up to four disposable tip racks can be placed on the deck’s black carriers (Figure 2). Each tip rack contains
96 x 1 mL disposable tips. At the time of loading (section 5.2.4), RoboSep™-16 will prompt the user to load
tips if the tip tracking system detects empty tip racks. After replacing a disposable tip rack, click on the
associated rack number on the loading screen.
If loading a partially filled tip rack, the starting tip position must be specified by clicking the corresponding
position on the Loading screen.
Note: Please ensure that the tip locations indicated on the Loading screen match those on the RoboSep™-16
deck.
In addition to transporting liquids, the disposable tips can be used to check whether caps are left on vials and
bottles, and whether sufficient reagent and buffer volumes are provided. There are options to check for caps
and volumes at the beginning of each run (section 4.1).
1.3.4 Waste Bin
If working with biohazardous samples wear gloves when handling all parts
of the instrument. Avoid touching tips discarded into the waste bin.
A waste bin is present on the deck (Figure 2) to collect used tips. A waste bag should be placed into the bin to
collect used tips.
The waste bin can hold the equivalent of 4 racks of tips. Ensure that the waste bin has sufficient space to
contain all tips used in a run.
To dispose and replace the waste bag:
1. Select [Exit] on the Home screen and wait until the RoboSep™-16 application turns off.
2. Turn off the RoboSep™-16 system using the instrument power switch.

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3. Open the RoboSep™-16 door to access the gantry (section 1.3).
4. Move the gantry (Figure 1) by hand to the left side.
5. Remove the waste bin from the deck.
6. Remove the bag retainer from the bin and dispose of the used waste bag.
7. Place a new waste bag in the bin and secure it with the bag retainer.
8. Place the waste bin back on the deck.
Note: The gantry will automatically move back into position at the beginning of the next run. Do not move the
gantry back manually.
1.3.5 Reagent Vial Rack
The reagent vial rack can hold up to 24 EasySep™ reagent vials (Figure 5). The rack is split into four groups
(1 - 4), corresponding to the reagents required for each individual protocol group. Locating pins under the rack
facilitate correct placement and orientation when loading onto the deck.
Figure 5. Reagent Vial Rack. (A) Empty and (B) loaded with EasySep™ vials. Note that the primary (top)
row should always be loaded.
Symbols on the reagent vial rack (Figure 6) correspond to the following:
□= Selection Cocktail
○= Primary Antibody (if required)
∆ = Magnetic Particles
Figure 6. Reagent Symbols and Protocol Group Numbers on the Reagent Vial Rack

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For each protocol group, up to two sets of reagent vials can be placed in the rack. Each row on the rack is
staggered at a different height to allow barcode scanning of all vials.
Note: Ensure reagents are loaded in the primary (top) row of the rack (Figure 5). To ensure that sufficient
reagents are available for a run, additional reagents can be placed in the backup (bottom) row of the rack.
1.4 Barcode Scanner
The barcode scanner is used to record reagent specific information such as catalog and lot numbers. This
information is recorded in the End of Run Report available at the end of every run (section 6.0).
The barcode scanner is mounted on the front arm of the gantry (Figure 1). At the start of each run, the
barcode scanner moves across the reagent vial rack and scans the 2D barcodes on each vial. The primary
(top) row of the reagent vial rack will be scanned first.
Note: To remove the barcode scanning option, edit the user profile hardware preferences to disable the “Scan
reagent barcodes prior to each run” option (section 4.1).
Optional: A handheld barcode scanner (e.g. Catalog #20501) can be connected to the computer via the USB
portal. This can be used to scan User IDs, sample IDs and buffer lot numbers, which are then recorded in the
End of Run Reports for each protocol that is run (section 6.0).
1.5 Computer Controller and RoboSep™-16 Application
The computer provided with RoboSep™-16 contains a graphical user interface that controls the operation of
the instrument.
The computer provided with RoboSep™-16 does not contain anti-virus
software. Users are encouraged to determine if anti-virus software is
required at their institution.
During operation of the instrument, any user-installed anti-virus software
should be turned off to prevent unnecessary interruption of the protocol run.
1.6 User Interface Overview
1.6.1 Screens
Five screens can be accessed using the TaskBar on the left of the application window, as shown in Figure 7.

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Figure 7. Screens Accessed from the Home Screen

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1.6.2 Icons
Table 2. Icons Used in the RoboSep™-16 Application
ICON DESCRIPTION ICON DESCRIPTION
Navigate to the Home screen Remove protocols
Preference selected Add protocol presets
Preference unselected Preview / View
Move to next step / View other
users
Run protocols
Go to previous screen Scan reagent barcodes
Sort from A to Z Pause run
Sort from Z to A Stop protocol
Save Load deck
Delete Unload
Add user profile Copy to USB
Copy user profile Additional information
Delete user profile Navigate to the Reports screen
Edit user profile Navigate to the Help screen
Change default user image Select option
Navigate to User Preferences
screen
Exit or cancel operation
List only EasySep™ Human
protocols
Enter Admin mode
List only EasySep™ Mouse
protocols
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Diagnostic Package