Phcbi MCO-170AICUVL User manual

Please read the operating instructions carefully before using this product, and keep the operating instructions
for future use.
See page 89 for model number.
MCO-170AICUVL
MCO-170AICUVHL
Operating Instructions
CO2Incubator

1
CONTENTS
INTRODUCTION .................................................................................................................3
PRECAUTIONS FOR SAFE OPERATION..........................................................................4
SYMBOLS ON INCUBATOR ...............................................................................................9
SAFETY ENVIRONMENTAL CONDITIONS .......................................................................9
INCUBATOR COMPONENTS ...........................................................................................10
Unit ............................................................................................................................... 10
LCD touch panel........................................................................................................... 12
Remote alarm terminal ................................................................................................. 14
INSTALLATION..................................................................................................................15
Installation site.............................................................................................................. 15
Installation..................................................................................................................... 17
Connecting CO2gas cylinder ....................................................................................... 20
BEFORE COMMENCING OPERATION ...........................................................................22
Initial cleaning method.................................................................................................. 22
Removing inner attachments........................................................................................ 23
Installing inner attachments .......................................................................................... 25
Filling humidifying pan .................................................................................................. 26
FOR BETTER CULTIVATION............................................................................................27
Precautions for cultures................................................................................................ 27
Preventing contamination ............................................................................................. 28
CORRECT OPERATION ...................................................................................................29
BASIC OPERATION ON LCD TOUCH PANEL .................................................................30
BASIC PARAMETERS ......................................................................................................32
Numerical input to input window................................................................................... 32
Setting temperature, CO2density and high limit temperature alarm............................ 33
Setting key lock ............................................................................................................ 35
Removing key lock ....................................................................................................... 37
ALARM PARAMETERS.....................................................................................................39
OPERATION/ALARM LOG................................................................................................42
Setting log interval ........................................................................................................ 42
Displaying operation log ............................................................................................... 43
Exporting operation log................................................................................................. 46
Displaying alarm log ..................................................................................................... 49
Exporting alarm log....................................................................................................... 51
OTHER PARAMETERS.....................................................................................................54
Setting date and time.................................................................................................... 54
Setting brightness and sleep ........................................................................................ 55

2
CONTENTS
UV LAMP PARAMETERS .................................................................................................57
Using UV lamp.............................................................................................................. 57
Setting UV lamp ON period .......................................................................................... 58
Lighting UV lamp for 24 hours ...................................................................................... 60
H2O2DECONTAMINATION ...............................................................................................62
H2O2decontamination .................................................................................................. 63
Precautions when handling H2O2reagent .................................................................... 67
ELECTRIC LOCK (OPTION).............................................................................................68
Setting User-ID ............................................................................................................. 68
Setting auto lock ........................................................................................................... 70
Unlocking the outer door .............................................................................................. 72
Unlocking the outer door during a power failure or malfunction................................... 73
Cancelling the auto lock setting.................................................................................... 74
GAS AUTO CHANGER (OPTION) ....................................................................................75
Connecting CO2gas cylinder ....................................................................................... 75
Automatic CO2gas supply line changeover ................................................................. 76
Manual CO2gas supply line changeover ..................................................................... 78
ROUTINE MAINTENANCE ...............................................................................................79
ALARMS, SAFETY, AND SELF-DIAGNOSIS....................................................................80
TROUBLESHOOTING.......................................................................................................83
DISPOSAL OF UNIT..........................................................................................................86
SPECIFICATIONS .............................................................................................................87
PERFORMANCE...............................................................................................................89
SAFETY CHECK SHEET ..................................................................................................90

3
INTRODUCTION
■Read the operating instructions carefully before using the Product and follow the instructions for safety
operation.
■PHC Corporation disavows any responsibility for safety if the Product is used for other than the intended
use or used with any procedures other than those given in the operating instructions.
■Keep the operating instructions in a suitable place so that it can be referred to as necessary.
■The contents of the operating instructions are subject to change without notice for improvement of
performance or functions.
■Contact our sales representative or agent if any page of the operating instructions is lost or the page
order is incorrect.
■Contact our sales representative or agent if any point in the operating instructions is unclear or if there
are any inaccuracies.
■No part of the operating instructions may be reproduced in any form without the expressed written
permission of PHC Corporation.
IMPORTANT NOTICE
PHC Corporation guarantees this product under certain warranty conditions. However, please note that
PHC Corporation shall not be responsible for any loss or damage to the contents of the product.
<Intended Use>
This equipment is designed for cell and tissue culture for laboratory use. Not for clinical diagnosis or
treatment of humans or animals.

4
PRECAUTIONS FOR SAFE OPERATION
It is imperative that the user comply with the operating instructions as
they contain important safety advice.
Items and procedures are described so that you can use this unit correctly and safely. If
the precautions advised are followed, this will prevent possible injury to the user and any
other person.
Precautions are illustrated in the following way:
WARNING
Failure to observe WARNING signs could result in a hazard to personnel
possibly resulting in serious injury or death.
CAUTION
Failure to observe CAUTION signs could result in injury to personnel and
damage to the unit and associated property.
Symbol shows;
This symbol means caution.
This symbol means an action is prohibited.
This symbol means an instruction must be followed.
WARNING
As with any equipment that uses CO2gas, there is a likelihood of oxygen depletion in the vicinity of
the equipment. It is important that you assess the work site to ensure there is suitable and sufficient
ventilation. If restricted ventilation is suspected, then other methods of ensuring a safe environment
must be considered. These may include atmosphere monitoring and warning devices.
USA Only (Model with a lamp): This product has a lamp that contains mercury. Disposal may be
regulated in your community due to environmental considerations. For disposal or information, please
visit PHC website: https://www.phchd.com .
Contains mercur
y
/ Contenu avec mercure
For more information on safe handling
procedures, the measures to be taken in
case of accidental breakage and safe
disposal options visit:
ec.gc.ca/mercure-mercury/.
Dispose of or recycle in accordance with
applicable laws.
Pour plus de renseignements sur les
procédures de manutention sécuritaire, les
mesures à prendre en cas de bris accidentel et
les options d’élimination sécuritaire visitez:
ec.gc.ca/mercure-mercury/.
Mettez au rebut ou recyclez conformément
aux lois applicables.
For the State of California, USA Only:
This product contains a CR Coin Cell Lithium Battery which contains Perchlorate Material – special
handling may apply. See www.dtsc.ca.gov/hazardouswaste/perchlorate.

5
Do not use the unit outdoors. Exposure to rain may cause leakage and/or electric shock.
Only qualified engineers or service personnel should install the unit. The installation by
unqualified personnel may cause electric shock or fire.
Install the unit in a location capable of bearing the total combined weight (product + optional
accessories + stored items). After installing the unit, be absolutely sure to take precautions to
prevent the unit from falling over. If the unit is installed in a location which is not strong enough or if
the proper precautions are not taken, the unit may fall over and cause injuries.
Do not install the unit where there are high levels of moisture or where it may be splashed with
water. Installing the unit where there are high levels of moisture or where it may be splashed with
water may cause the insulation to deteriorate and give rise to leakage and/or electric shock.
Do not install the unit in a location where flammable or volatile substances are present.
Installing the unit in a location where flammable or volatile substances are present may cause
explosions and/or a fire.
Do not install the unit in a location where corrosive gases such as acids are present. Installing
the unit in a location where corrosive substances are present may cause electric components to
corrode, leading to leakage and/or electric shock due to the deterioration of insulation resulting from
corroded electrical components.
Do not place this unit in a location where it is difficult to disconnect the power supply plug.
Failure to disconnect the power supply plug may cause fire in the event of a problem or malfunction.
Be absolutely sure to earth (ground) the unit in order to prevent electric shock. Failure to earth
the product may give rise to electric shock. If necessary, ask a qualified contractor to do this work.
Do not connect the earth wire to a gas pipe, water pipe, or lightning rod when earthing the
unit. Earthing the unit improperly may give rise to electric shock.
Connect the unit to a power source as indicated on the rating label attached to the unit. Use of
any other voltage or frequency other than that on the rating label may cause fire or electric shock.
Never store volatile or flammable substances in this unit except in a sealed container. Such
substances may cause explosion or fire if they leak.
Never insert metal objects such as pins and wires into any vent, gap or outlet on the unit. This
may cause electric shock or injury by accidental contact with moving parts.
When handling harmful samples (for example, those which consist of toxic, pathogenic, or
radioactive substances), install the unit inside a designated isolation facility. If the unit is
installed in a location which is not an isolation facility, there may be detrimental effects on both people
and the natural environment.
WARNING

6
PRECAUTIONS FOR SAFE OPERATION
Before proceeding with maintenance or checking of the unit, set the power switch to OFF and
disconnect the power supply plug. Performing the work while power is still flowing to the product or
while the power-supply plug is still connected may give rise to electric shock and/or injury.
Do not touch any electrical parts (such as power supply plug) or operate switches with a wet
hand. This may cause electric shock.
Wear protective gloves and mask during maintenance. Touching or inhaling chemicals or
aerosols from around the unit may be detrimental to health.
Never splash water directly onto the unit as this may cause electric shock or short circuit.
Never put containers with liquid on top of the unit as this may cause electric shock or short circuit if
the liquid is spilled.
Never damage the power supply cord or power supply plug (by breaking, adapting, placing near
a source of heat, bending with force, twisting, pulling, adding weight, or binding). A damaged
power supply cord or power supply plug may cause electric shock, short circuit, or fire.
Never disassemble, repair, or modify the unit yourself. A high-voltage area is located inside the
unit. Any work carried out by an unauthorized personnel may result in electric shock. Contact our
sales representative or agent for maintenance or repair.
Make sure the power supply plug is pushed fully in. Faulty insertion of the power supply plug may
cause electric shock or fire due to generation of heat. Never use a damaged power supply plug or
loose power outlet.
Disconnect the power supply plug if there is anything wrong with the unit. Continued abnormal
operation may cause electric shock or fire.
Grip the power supply plug when disconnecting the power supply cord from the outlet. Pulling
the power supply cord may cause electric shock or short circuit.
Remove dust from the power supply plug periodically. Dust on the power supply plug may cause
insulation failure due to moisture and thus cause a fire. Disconnect the power supply plug and wipe it
with a dry cloth.
Disconnect the power supply plug before moving the unit. Take care not to damage the power
supply cord. A damaged cord may cause electric shock or fire.
Disconnect the power supply cord when the unit is not in use for long periods. Keeping the unit
connected may cause electric shock, leakage, or fire due to the deterioration of insulation.
WARNING

7
If the unit is to be stored unused in an unsupervised area for a long period, ensure that children do
not have access and that doors cannot be closed completely.
Ask a qualified contractor to carry out disassembly and disposal of the unit. Leaving the unit in
a location that can be accessed by third parties may result in unexpected accidents (e.g. the unit may
be used for unintended purposes).
Do not leave the plastic bags used for packing in a place where they can be reached by small
children as this may result in unexpected accidents such as suffocation.
Use the reagent specified by our company for H2O2decontamination. Using a different H2O2
solution may result in explosion or damage to the incubator.
When performing H2O2decontamination, securely close the internal and external doors. Failure to
do so may be harmful to health due to leakage of H2O2gas.
During H2O2decontamination, plug the access hole with the silicon cap that is provided. Failure to
do so may be harmful to health due to leakage of H2O2gas.
Always use the removal power supply cord that is provided. Other power supply cord may cause
electric shock or fire.
Do not use the supplied power cord for other electrical equipment. Such power supply cord may
cause fire or electric shock.
When using CO2gas for control, make sure that there is adequate ventilation. Using CO2gas in a
small room without adequate ventilation may cause gas poisoning or oxygen deprivation. In addition,
when opening the incubator doors, do not directly inhale the air in the chamber.
When connecting a gas cylinder to the incubator, confirm the gas type. Confirm that the
connections are secure and that no gas will leak. Be sure to use the specified pressure. Using
an incorrect gas or pressure may result in explosion or fire, or in gas poisoning or oxygen deprivation
due to gas leak.
Install the incubator in a location with adequate ventilation. If adequate ventilation cannot be
provided, then install an alarm system using CO2and O2 densitometers.
Do not look directly at UV light. UV light is harmful to the eyes.
Never start H2O2decontamination when “Warning: UV Bulb Life” is displayed in the message display
field. The UV resolve is not sufficient.
Do not unlock the outer door using the accessory key during H2O2decontamination or during
H2O2gas resolve by UV. Doing so may cause harm to health from H2O2gas leakage.
The CO2incubator must be dismantled and disposed of by qualified personnel only. If the CO2
incubator is left where outsiders enter, it may result unexpected accident (for example, children to
become locked inside).
WARNING

8
PRECAUTIONS FOR SAFE OPERATION
This unit must be plugged into a dedicated circuit protected by branch circuit breaker.
Use a dedicated power source as indicated on the rating label attached to the unit. A multiple-tap may
cause fire resulting from abnormal heating.
Never store corrosive substances such as acid or alkali in this unit if the container cannot be
sealed. These may cause corrosion of inner components or electric parts.
Check the setting when starting up of operation after power failure or turning off of power
switch. The stored items may be damaged due to the change of setting.
Be careful not to tip over the unit during movement to prevent damage or injury.
Prepare a safety check sheet (copy the last page) when you request any repair or maintenance for
the safety of service personnel.
Wear rubber gloves when handling the H2O2reagent. Direct contact with the H2O2reagent may
result in inflammation of the skin.
H2O2decontamination can be performed only for the chamber and chamber attachments with standard
specifications, and not for any other objects.
Perform H2O2decontamination with the chamber attachments arranged as specified by our
company. Arranging them in a different way may result in insufficient decontamination.
After H2O2decontamination has been completed, wear rubber gloves and use a non-woven cloth to
wipe off the residual H2O2fluid from the bottom of the chamber, any objects that were
decontaminated, and the bottoms of ducts.
Do not climb on top of the incubator or place any object on it. By doing so you may damage the
incubator or knock it over, which may result in injury.
Wear rubber gloves when performing maintenance on the chamber. Failure to wear gloves may
result in cuts or abrasions from sharp edges or corners.
Do not damage the glass or give it a shock. The Inner door in the CO2Incubator are tempered glass,
but they can be broken or cause injury if they are used incorrectly.
CAUTION

9
SYMBOLS ON INCUBATOR
The following symbols are attached to the incubator.
This symbol indicates possibility of an electric shock. High-voltage electrical
components are placed under the covers. Only a qualified engineer or service
personnel should be allowed to open these covers.
This symbol indicates an ultraviolet light (UV) caution.
This symbol indicates that caution is required. Refer to product documentation for
details.
This symbol indicates a hot surface.
This symbol indicates an earth.
This symbol means “ON” for a power switch.
This symbol means “OFF” for a power switch.
SAFETY ENVIRONMENTAL CONDITIONS
This equipment is designed to be safe at least under the following conditions (based on the IEC 61010-1):
■Indoor use;
■Altitude up to 2,000 m;
■Temperature 5 oC to 40 oC
■Maximum relative humidity 80 % for temperature up to 31 oC decreasing linearly to 50 % relative
humidity at 40 oC;
■Mains supply voltage fluctuations up to ±10 % of the nominal voltage;
■Transient overvoltages up to the levels of OVERVOLTAGE CATEGORY II;
■Temporary OVERVOLTAGES occurring on the mains supply;
■Applicable pollution degree of the intended environment (POLLUTION DEGREE 2 in most cases);
* Above conditions do not indicate the performance of this product. For the performance of this product,
refer to "PERFORMANCE" on page 89.

10
INCUBATOR COMPONENTS
Unit
1 2 3 Tray 4 5
6
7
8
13
12
*1 Electric lock*1 11 10 9
*1: MCO-170AICUVHL or when an optional electric
lock MCO-170EL is installed.
*2: Type FG-7P or FG-7PL.
(When some inner
attachments are removed)
20
21
Access port
cover Silicon cap
Rear side
(When 13 is removed)
Sample air outlet cap
17 16 15 14 13
Removable power
supply cord port
Lower right side
19
Leveling feet
Handles

11
1. Outer door: The outer door is held to the frame with the magnetic seal. The door heater is installed in
the door panel. The door opening is reversible. Contact our sales representative or agent to change the
door hinge from left to right or vice versa.
2. Inner door: The inner door is made of tempered glass. However, do not subject the glass to excessive
impacts. The inner door can be changed to an optional small door (MCO-170ID). Contact our sales
representative or agent.
3. Tray catches: Insert tray to fit the concave portion on chamber.
4. Fan cover: The fan cover serves as the inlet for circulating air. It is removable.
5. Duct: The duct for the path for circulating air. It is removable.
6. Fan (inside the duct): The fan is made from polypropylene resin. It can be disinfected in an autoclave.
7. UV lamp: This UV lamp does not generate ozone. Never look directly at the UV light. Refer to pages
57~61 for using. For replacement, contact our sales representative or agent.
8. Humidity control bar: Reduces condensation in the unit caused by the external environment and
frequent door openings.
9. Humidifying pan cover: Prevents the UV light from leaking. Always use it. Using without it may have a
bad influence on the chamber temperature distribution and humidity recovery.
10. Humidifying pan: Fill the humidifying pan with sterile distilled water, and set the humidifying pan with
the inner side flush against the back. Install the humidifying pan in a longitudinal direction as its shorter
side is placed in the back.
11. Door switch: Detects the door opening/closing and stops the fan and electromagnetic valve for CO2
when the door is open. The UV lamp*1is also deactivated by the door opening.
12. Key hole*1:Hole for the accessory key to unlock the outer door while locked by the electric lock.
13. Switch cover: Prevent the accident of gas tube disconnected by the unexpected touch or power off.
14. Power supply cord cover plate: This plate is to prevent the removable power supply cord being
come off.
15. Connecting port A for CO2gas pipe: Refer to pages 20~21 for gas cylinder connection. Ensure that
the gas pressure is set at 0.03 MPa(G) ~0.1 MPa(G) (0.3 kgf/cm2(G) ~1 kgf/cm2(G), 4.4 psi(G) ~14.5
psi(G)).
Note: When the optional MCO-21GCP gas auto changer is installed, both ports A and B are available.
Refer to page 75 for gas auto changer.
16. Glow starter*2: The glow is started for the UV lamp.
17. Power switch: The power switch of this incubator (ON-“I”, OFF-“O”). It also functions as an
overcurrent breaker.
18. Sample air outlet: The sample air outlet also functions as an internal gas outlet. Normally, cover this
outlet with the sample air outlet cap.
19. USB port: Insert a USB flash drive to export operation and alarm logs. Refer to pages 42~53.
Note: USB flash drives with capacity of 32 GB or less that employ the FAT16/FAT32 file system are
supported. USB flash drives that require passwords cannot be used. Do not insert devices other than USB
flash drives into the USB port.
20. Access port: Place the silicon caps on both outside and inside of the port when the port is not being
used.
21. Remote alarm terminals: This terminal sends the alarm to a remote location by connecting to external
alarm unit. Refer to page 14.

12
INCUBATOR COMPONENTS
LCD touch panel
When sterilizing and cleaning the control panel, follow the precautions below.
(1) Do not spray liquid on the control panel directly.
(2) When sterilizing and cleaning, wipe the surface using a piece of gauze moistened with a proper
amount of disinfectant
(
the amount that cannot form droplets
)
.
The following display (called the Top screen) will appear when the power switch is turned ON.
Note: It takes approximately 20 seconds until Top screen is displayed. During warming-up, “Status: Gas
sensor initializing” is displayed in the Message display field (13), and “--.-” is displayed in the Present CO2
density display field (4).
2 6 7 8
3
1
1. Present temperature display field
The current chamber temperature is displayed.
2. Set temperature value display field
The set value of chamber temperature is
displayed. Default setting: 37 oC.
3. Heating indicator
This lamp lights when the heater is energized.
4. Present CO2density display field
The current chamber CO2density is displayed.
Nothing is displayed when CO2 density is set 0 %.
5. Set CO2density value display field
The set value of the chamber CO2density is
displayed. Default setting: 0 %.
6. CO2gas injection indicator
This lamp lights when CO2gas is being
injected.
7. CO2gas supply line indicator A and B*1
Current supplying CO2gas supply line
(connecting port for CO2gas pipe) is
displayed. The connecting port A/B for gas
pipe that is currently supplying CO2is
displayed in reverse video and blinks.
8. CO2gas supply line select key*1
This is a key to select CO2gas supply line A
or B (Connecting port A or B for CO2gas
pipe). When an optional gas auto changer
MCO-21GCP is installed, CO2 gas supply line
A/B changes over automatically when CO2
gas cylinder is empty. Changeover is also
workable by pressing this key.
*1: Only when an optional component MCO-21GCP (Gas auto changer) is installed, this key is workable. They
are not displayed when the MCO-21GCP is not installed.
5
4

13
10 11 9 12
13
18 17 16 15
9. Present date/time display field
Normally, this indicator shows date and time.
The date and time is simply set when the
incubator is shipped from the factory. Refer to
page 54 for details.
10. Over heat display
High limit temperature alarm is activated:
“Over Heat” is displayed alternately in normal
characters and reverse video.
11. UV lamp condition display
UV lamp ON: “UV : On” is displayed.
UV lamp OFF: “UV : Off” is displayed.
12. Outer door (opening/closing) display
Open: “Door : Open” is displayed alternately
in normal characters and reverse video.
Close: “Door : Closed” is displayed.
Locked: “Door : Locked” is displayed.*2
13. Message display field
Alarms, errors or messages are displayed
when fault occurs. Refer to pages 80~82.
Note: When there are a number of
alarms/errors, the display shows the message.
For example, if 2 alarms/errors occur in total,
the display shows “1/2”.
14. Message select key
When there are a number of alarm/errors, the
message on the screen is changeable.
15. Menu key
Press this key to display the Menu screen. It is
possible to set various setting on the Menu
screen. Refer to page 30.
14

14
INCUBATOR COMPONENTS
16. H2O2key*2
This key is to run H2O2 decontamination. Refer
to pages 62~67.
17. Unlock key*3
Press this key is to unlock the outer door when
it is auto-locked by electric lock. Refer to page
73. When the auto lock function is OFF, this
key is not displayed.
18. Buzzer key
Press this key to silence the buzzer. However,
when the ring back is ON, the buzzer will
sound again when the ring back passed and
the alarm state still continues. Refer to pages
40~41 and 80~82.
Note: It is not possible to silence the buzzer
for the high limit temperature alarm.
*2: The H2O2 decontamination function is workable MCO-170AICUVHL or when all H2O2generator MCO-HP,
H2O2decon board MCO-170HB and electric lock MCO-170EL are installed in the MCO-170AICUVL. If not, the
H2O2 key is not displayed on the LCD touch panel.
*3: Auto lock function by electric lock is workable MCO-170AICUVHL or when an optional electric lock
MCO-170EL is installed. If not, “Door : Locked” or Unlock key are not displayed.
Remote alarm terminal
The alarm of this product can be informed at a remote location from this product by connecting the external
alarm unit to the remote alarm terminals. For the type and behavior of remote alarm output, refer to pages
80~82.
The terminal of the remote alarm is installed at the
rear upper right of the unit (see the right figure). The
alarm is outputted from this terminal. Contact
capacity is DC 30 V, 2 A.
When Buzzer key is pressed, the behavior of the
remote alarm is showed in Table 1.
Note:
• In the door alarm, the remote alarm does not work.
Refer to pages 80 and 81.
• It is recommended to use standard signal and
interface cables with a maximum length of 30
meters.
Table 1. The behavior of the remote alarm when pressing the Buzzer key
Remote Alarm setting
(Refer to pages 39~41)
Connecting
terminal
Normal
condition
Abnormal condition
(Including in the cases of power outage and
of where the power suppl
y
plu
g
is pulled out.
)
When pressin
g
the buzzer ke
y
ON:
Non-interlock with Buzzer key
COM.-N.C. Close Open Open
(
Maintain in abnormalit
y)
*
COM.-N.O. Open Close Close
(
Maintain in abnormalit
y)
*
OFF:
Interlock with Buzzer key
COM.-N.C. Close Open Close
(
Return to normal
)
COM.-N.O. Open Close Open
(
Return to normal
)
*In case of Err01 (CO2 gas cylinder empty), Err11, 12(CO2 sensor error), the condition returns to normal.
Remote alarm
terminal

15
INSTALLATION
Installation site
For correct operation of the incubator, install it in a location with the following conditions.
WARNING
When using CO2gas for control, make sure that there is an adequate ventilation. Using CO2gas in a
small room without adequate ventilation may cause gas poisoning or oxygen deprivation. In addition, when
opening the incubator doors, do not directly inhale the air in the chamber.
Si l’appareil est utilisé dans un evdroit restreint, le niveau de la densite CO2de l’air peut s’élever et peut
être nocif aux humains. Evitez d’aspirer l’air provenant de l’inérieur de l’appareil quand vous ouverz la
porte.
Normal air environment
Install the incubator in an environment with normal air.
Do not expose to direct sunlight
Do not install the incubator in a location where it will be exposed to direct sunlight. If the incubator is
operated in direct sunlight, performance will be adversely affected.
Separate from heat sources
Do not install the incubator near significant heat sources, such as heaters, boilers, ovens, or autoclaves.
Heat will adversely affect the performance of the incubator.
Ambient temperature at least 5 °C lower than set temperature
The control temperature of the incubator is at least 5 °C higher than the ambient temperature. For example,
if the chamber is controlled at 37 °C, the ambient temperature must be 32 °C or less. Do not allow the
ambient temperature to become too high.
Strong and level floor
Select a site with a strong and level floor. If the floor is uneven or the installation is not level, the incubator
will be unstable and this may cause accident or injury. To avoid vibration and noise, always make sure that
the installation is stable. An unstable surface may result in vibration or noise.
WARNING
Install the incubator at a location that can support the weight. If the floor is not strong enough or if the
installation is insufficient, the incubator may fall over and cause injury.
Always make sure that the floor is strong, even, and level, and that the incubator will not tip over.
A
n insufficient installation may result in injury due to water leakage or the incubator falling over.
Separate from vibration products
Do not install the incubator near vibration products. Vibration may cause culture failure.

16
INSTALLATION
Low humidity
Select a site with a relative humidity of 80 %R.H. or lower. Using the incubator in high humidity may result
in current leakage or electric shock.
WARNING
Do not use the incubator outdoors. If the incubator is exposed to rain water, it may result in current
leakage or electric shock.
Never install the incubator in a moist location, such as near a sink or water line, or where it is likely
to be exposed to water. In addition, do not install it near water or steam pipes. Moisture can cause the
insulation to deteriorate, which may result in current leakage or electric shock.
No inflammable or corrosive gas
Never install the incubator in a location where it will be exposed to inflammable or corrosive gas. Doing so
may result in explosion or fire. In addition, insulation may deteriorate due to corrosion of protective casing,
resulting in current leakage or electric shock.
No falling objects
Do not install the incubator in a location where there is the possibility of objects falling from above. Doing
so may result in damage or accident.

17
Installation
1. Remove the packing tape and clean up.
Remove all the tapes that are securing the doors and the inner attachments. Open the doors for ventilation.
If the outer panels are dirty, wet a cloth with a diluted neutral detergent and wipe them. (Undiluted
detergent can damage the plastic components. For the dilution, refer to the instruction of the detergent.)
Wipe off the residual detergent with a wet cloth and then wipe off any moisture.
Note: Remove the cable tie banding the power supply cord. Prolonged banding may cause the corrosion
of the cord coating.
WARNING
Do not leave the plastic wrapping bags within reach of children. If the bag is placed over a child’s
head, it can block the mouth and nose and cause suffocation.
2. Set the humidifying pan and humidifying pan cover (Fig. 1).
3. Set 4 trays (Fig. 2).
(When the optional small door (MCO-170ID) is installed)
To effectively use the gastight split doors, set 3 trays at the same level as bottom side of each gastight split
door (Fig. 3).
Humidif
y
in
g
pan
Fig. 1
Humidifying pan cover
4 Trays
Fig. 2
Gastight split door
At the same level
as bottom side of
each gastight split
door
Small door
Fig. 3

18
INSTALLATION
4. Adjust the leveling feet.
Adjust the leveling feet by turning them
counterclockwise to level the incubator (Fig. 4).
Note: Incubating on a leaning tray may have a bad
influence on the cultivation.
5. Ground the incubator.
Ground the incubator during installation to prevent electric shock in case the insulation is not sufficient. If
there is no ground wire at the location, consult with qualified service personnel.
When a ground must be installed
If a grounded 3-pole outlet is not available, then a ground must be installed. Consult with qualified service
personnel.
WARNING
To prevent electric shock, always ground the incubator. If grounding is not possible, then have a ground
installed by qualified personnel. If the incubator is not grounded, it may result in electric shock.
Never connect the ground wire to a gas pipe, water pipe, lightning rod, or telephone ground wire.
Doing so may cause electric shock.
Installing a ground fault circuit breaker
If using the incubator in the location with moisture or humidity cannot be avoided, then it is recommended
that a ground fault circuit breaker be installed in the power supply circuit (i.e., the power supply at the
incubator). Have the circuit breaker installed by qualified service personnel.
CAUTION
Do not climb on the incubator or place objects on top of it. Doing so may damage it or cause it to fall
over, resulting in injury.
Fi
g
. 4
Levelin
g
fee
t
Shrink
Stretch