Sutter Instrument Lambda 721 User manual

One Digital Drive
Novato, CA 94949
Voice: 415-883-0128 Web: www.sutter.com
Fax: [phone] Email: [e-mail]
Lambda 721
Ultra-High-Speed Optical Beam Combined
7-Channel Wavelength Switching
LED Light Source System
With TTL & USB Interface for External Control
Operation Manual
Rev. 1.10c ((20210517)

ii
LAMBDA 721 OPERATION MANUAL – REV. 1.10C (20210517)
Copyright © 2021 Sutter Instrument Company. All Rights Reserved.
LAMBDA-10®and SmartShutter®are registered trademarks of Sutter Instrument
Company.

LAMBDA 721 OPERATION MANUAL – REV. 1.10C (20210517)

LAMBDA 721 OPERATION MANUAL – REV. 1.10C (20210517)
iii
DISCLAIMER
The Lambda 721 is an ultra-high-speed wavelength switching multi-LED-based beam
combined illumination or light source device. The purpose of the system is to be an
illuminator for microscopes. No other use is recommended.
This instrument is designed for use in a laboratory environment. It is not intended, nor
should it be used in human experimentation or applied to humans in any way. This is not a
medical device.
Unless otherwise indicated in this manual or by Sutter Instrument Technical Support for
reconfiguration, do not open or attempt to repair the instrument.
Do not allow unauthorized and/or untrained operative to use this device.
Any misuse will be the sole responsibility of the user/owner and Sutter Instrument Company
assumes no implied or inferred liability for direct or consequential damages from this
instrument if it is operated or used in any way other than for which it is designed.
SAFETY WARNINGS AND PRECAUTIONS
Electrical
Operate the Lambda 721 using 110 – 240 VAC., 50-60 Hz line voltage. This instrument is
designed for use in a laboratory environment that has low electrical noise and mechanical
vibration. Surge suppression is recommended at all times.
Fuse Replacement: Replace only with the same type and rating:
5 x 20 mm glass tube, Time Lag (IEC 60127-2, Sheet 6)
T3.15A 250V (Time Lag, 3.15 Amps, 250 Volts)
(Littelfuse 02193.15MXAP (219XA Series, RoHS compliant))
A spare fuse is located in the power input module.
Avoiding Electrical Shock and Fire-related Injury
Always use the grounded power supply cord set provided to connect the system’s
power adapter to a grounded/earthed outlet (3-prong). This is required to protect you
from injury in the event that an electrical hazard occurs.
Do not disassemble the system. Refer servicing to qualified personnel.
To prevent fire or shock hazard do not expose the unit to rain or moisture.
Electromagnetic Interference
To comply with FDA and CE/EU electromagnetic immunity and interference standards; and
to reduce the electromagnetic coupling between this and other equipment in your lab always
use the type and length of interconnect cables provided with the unit for the interconnection
of its components (see the Technical Specifications appendix for more details).
Operational
Failure to comply with any of the following precautions may damage this device.

LAMBDA 721 OPERATION MANUAL – REV. 1.10C (20210517)
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Operate only in a location where there is a free flow of fresh air on all sides.
NEVER ALLOW THE FREE FLOW OF AIR TO BE RESTRICTED.
This instrument is designed for operation in a laboratory environment (Pollution Degree
I) that is free from mechanical vibrations, and electrical noise and transients.
DO NOT CONNECT OR DISCONNECT THE LQUID LIGHT GUIDE BETWEEN
THE LAMBDA 721 AND MICROSCOPE ADAPTER (OR THE ADAPTER FROM THE
MICROSCOPE) WHILE POWER IS ON.
Operate this instrument only according to the instructions included in this manual.
Do not operate if there is any obvious damage to any part of the instrument.
Do not operate this instrument near flammable materials. The use of any hazardous
materials with this instrument is not recommended and, if undertaken, is done so at the
users’ own risk.
Do not operate if there is any obvious damage to any part of the instrument.
Avoiding Physical Injury while Powered up and Emitting Light
INFRARED AND ULTRAVIOLET RADIATION: Possible infrared and ultraviolet
radiation generated by this lamp can cause significant skin burns and eye damage.
Other
Retain the original packaging for future transport of the instrument.
Sutter Instrument reserves the right to change specifications without prior notice.
This device is intended only for research purposes.
WARNING
DO NOT LOOK DIRECTLY INTO THE OUTPUT OF THE LIGHT
APERTURE MOUNTED ON THE CONTROLLER, THE
CONNECTED LIQUID LIGHT GUIDE, OR THE ATTACHED
MICROSCOPE ADAPTER! Always direct the output of the light
aperture into the microscope using the appropriate adapters,
directed away from anyone’s eyes, and not directed toward any
reflective surface.

LAMBDA 721 OPERATION MANUAL – REV. 1.10C (20210517)
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TABLE OF CONTENTS
DISCLAIMER ......................................................................................................................................iii
SAFETY WARNINGS AND PRECAUTIONS...................................................................................iii
Electrical .................................................................................................................................................iii
Avoiding Electrical Shock and Fire-related Injury.............................................................................iii
Electromagnetic Interference ...............................................................................................................iii
Operational .............................................................................................................................................iii
Avoiding Physical Injury while Powered up and Emitting Light .....................................................iv
Other........................................................................................................................................................iv
1. INTRODUCTION.............................................................................................................................1
1.1 Introduction.......................................................................................................................................1
1.1.1 How to Use this Manual............................................................................................................1
1.1.2 Technical Support ......................................................................................................................1
1.2 General Description..........................................................................................................................2
1.2.1 Features.......................................................................................................................................3
1.2.2 Common Applications................................................................................................................3
2. INSTALLATION ..............................................................................................................................5
2.1 Unpacking..........................................................................................................................................5
2.2 Getting Started..................................................................................................................................5
2.3 Configuration ....................................................................................................................................7
DIP Switches ........................................................................................................................................7
3. OPERATIONS ..................................................................................................................................9
3.1 Controls..............................................................................................................................................9
3.1.1 Power Switch..............................................................................................................................9
3.2 Indicators ...........................................................................................................................................9
3.2.1 LED On/Off State Indicators ....................................................................................................9
3.3 Connectors .........................................................................................................................................9
3.3.1 Light Beam Combiner Output..................................................................................................9
3.3.1 TTL Inputs (LED # 1 – 7) BNC Connectors ........................................................................10
3.3.1 Ring Buffer BNC Connector ...................................................................................................10
3.3.2 USB Device Interface Connector............................................................................................10
3.4 Operation .........................................................................................................................................10
3.1 Lambda 10 Mode.............................................................................................................................10
3.1.1 Control Operations...................................................................................................................11
3.1.2 Mode Exit ..................................................................................................................................11
3.1 TTL Mode ........................................................................................................................................11
3.1.1 Control Operations...................................................................................................................12
3.1.1 Mode Exit ..................................................................................................................................12
3.2 Ring Buffer Mode............................................................................................................................12
3.2.1 Running the Ring Buffer Control Operations ......................................................................13
3.2.2 Stopping the Ring Buffer Run ................................................................................................13
3.2.1 Editing the Ring Buffer ...........................................................................................................13
3.1 Setup Mode ......................................................................................................................................13
3.1 Demo Mode......................................................................................................................................14

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4. EXTERNAL CONTROL OPERATIONS......................................................................................15
4.1 USB Interface..................................................................................................................................15
4.1.1 Virtual COM Port (VCP) Serial Port Settings......................................................................15
4.2 Handshaking ...................................................................................................................................15
4.3 Commands .......................................................................................................................................16
4.3.1 Lambda 10-x Mode (‘L’ or ‘l’) Command ..............................................................................16
4.3.1.1 Lambda 10 Series Compatible LED Selection Commands...........................................16
4.3.1 TTL Mode (‘T’ or ‘t’) Command.............................................................................................18
4.3.1 Start Ring Buffer Run (‘R’ or ‘r’) Command ........................................................................19
4.3.1.1 Return Values while Running the Ring Buffer..............................................................19
4.3.1 Stop TTL Mode or Ring Buffer Run (‘O’ or ‘o’) Command.................................................19
4.3.1 Load Ring Buffer (‘B’ or ‘b’) Command.................................................................................20
4.3.1.1 ‘B’ or ‘b’ Command Stop Loading Ring Buffer Entry Value........................................20
4.3.1.1 ‘B’ or ‘b’ Command Ring Buffer Entry Values ..............................................................20
4.3.1 Turn on a discrete LED or a group of LEDs (‘M’ or ‘m’) Command .................................21
4.3.1 Set LED Power Level (‘P’ or ‘p’) Command .........................................................................22
4.3.1 Get LED ON/OFF Status (‘S’ or ‘s’) Command ...................................................................22
4.3.1 Get Lambda 10-3 Compatible Controller Type and Configuration Command.................22
4.3.1 Get Lambda 10-3 Compatible Status Command..................................................................23
5. MAINTENANCE AND RECONFIGURATION...........................................................................25
5.1 Routine Maintenance .....................................................................................................................25
5.2 Reconfiguration...............................................................................................................................25
5.2.1 Replacing and Installing New LED Cubes ............................................................................25
5.2.2 Setting up the Maximum Current Level for each LED .......................................................25
APPENDIX A. LIMITED WARRANTY............................................................................................27
APPENDIX B. ACCESSORIES .........................................................................................................29
B.1. Liquid Light Guides, SMA Fiber, and Other Accessories.........................................................29
B.2. Microscope Mounting Adapters...................................................................................................29
B.3. LED Cubes .....................................................................................................................................29
APPENDIX C. FUSE REPLACEMENT...........................................................................................31
APPENDIX D. TECHNICAL SPECIFICATIONS ...........................................................................33
APPENDIX E. QUICK REFERENCE ..............................................................................................35
E.1. Manual Operation.........................................................................................................................35
E.2. Configuration.................................................................................................................................36
E.3. External Control............................................................................................................................36
INDEX .................................................................................................................................................49
TABLE OF FIGURES
Figure 1-1. Lambda 721 indicators, controls, and connectors............................................................... 2
Figure 1-2. Stages of pass-through and reflection from each LED cube light source......................... 3
Figure 1-3. Lambda 721 LED Spectra Options. ...................................................................................... 4
Figure 2-1. Light guide installation. ......................................................................................................... 5

LAMBDA 721 OPERATION MANUAL – REV. 1.10C (20210517)
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Figure 2-2. Rear panel showing power entry module receptacle and switch....................................... 6
Figure 2-1. Rear panel showing connectors for TTL input for all 7 LED channels, Ring Buffer
strobe input, and USB for external control. .......................................................................... 7
Figure 2-2. Left-side panel and DIP switches.......................................................................................... 7
Figure 3-1. Locations of individual indicators, controls, and connectors on the Lambda 721
controller front panel. .............................................................................................................. 9
Figure 3-2. Locations of power switch and individual connectors on the Lambda 721 controller
rear panel................................................................................................................................... 9
Figure 3-3. Startup screen. ...................................................................................................................... 10
Figure 3-4. Product and firmware revision screen. .............................................................................. 10
Figure 3-5. Lambda 10 Mode prompt..................................................................................................... 10
Figure 3-6. Lambda 10 Mode prompt..................................................................................................... 11
Figure 3-7. Lambda 10 Mode screen....................................................................................................... 11
Figure 3-8. Use TTL Mode prompt......................................................................................................... 11
Figure 3-9. TTL Mode start screen......................................................................................................... 11
Figure 3-10. TTL Mode running screen................................................................................................. 12
Figure 3-11. Use Ring Buffer prompt..................................................................................................... 12
Figure 3-12. Run existing Ring Buffer prompt. .................................................................................... 12
Figure 3-13. Ring buffer running screen................................................................................................ 12
Figure 3-14. Edit ring buffer query screen. ........................................................................................... 13
Figure 3-15. Ring buffer entry #1 screen. ............................................................................................. 13
Figure 3-16. Setup Mode prompt screen................................................................................................ 13
Figure 3-17. Edit ring buffer query screen. ........................................................................................... 14
Figure 3-18. Demo Mode prompt screen................................................................................................ 14
Figure 3-19. Enter Demo Mode prompt screen..................................................................................... 14
Figure 3-20. Demo Mode running screen............................................................................................... 14
Figure 5-1. LED cube placement. ........................................................................................................... 25
Figure 5-2. Setting the maximum current for an LED. ....................................................................... 26
Figure 5-3. Prompt for finishing setting LED current levels. ............................................................. 26
Figure 5-4. Message to turn off unit and reset DIP Switch 8 position before turning unit back on.26
Figure 5-5. Lambda 10 Mode prompt..................................................................................................... 26
Figure C-1. Power entry module and fuse detail................................................................................... 31
Figure E-1. Lambda 721 front panel and controls................................................................................ 35
Figure E-2. Lambda 721 menu navigation diagram............................................................................. 35

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Figure E-3. Lambda 721 rear panel, connectors, and controls............................................................ 36
Figure E-4. Lambda 721 left-side panel and DIP switches. .................................................................36
Figure E-5. LED cube placement............................................................................................................ 36
TABLE OF TABLES
Table 2-1. Configuration Switches 1 – 8 definitions. .............................................................................. 8
Table 4-1. USB-VCP interface serial port settings. .............................................................................. 15
Table 4-2. Lambda 10-x Mode (‘L’ or ‘l’) Command............................................................................. 16
Table 4-3. Lambda 10 Series compatible LED selection commands................................................... 17
Table 4-4. LED selection commands using ASCII digits...................................................................... 18
Table 4-5. TTL Mode (‘T’ or ‘t’) Command........................................................................................... 18
Table 4-6. Start Ring Buffer Run (‘R’ or ‘r’) Command....................................................................... 19
Table 4-7. Return values while running the Ring Buffer..................................................................... 19
Table 4-8. Stop TTL Mode or Ring Buffer Run (‘O’ or ‘o’) Command............................................... 19
Table 4-9. Load Ring Buffer (‘B’ or ‘b’) Command............................................................................... 20
Table 4-10. ‘B’ or ‘b’ Command Stop Loading the Ring Buffer entry value..................................... 20
Table 4-11. ‘B’ or ‘b’ Command Ring Buffer entry values................................................................... 20
Table 4-12. Change the on/off state of one or more LEDs (‘M’ or ‘m’) Command............................ 21
Table 4-13. Set LED Power Level (‘P’ or ‘p’) Command. .................................................................... 22
Table 4-14. Get LED ON/OFF Status (‘S’ or ‘s’) Command................................................................ 22
Table 4-15. Get Lambda 10-3 Compatible Controller Type and Configuration Command. ............ 23
Table 4-16. Get Lambda 10-3 Compatible Controller Type and Configuration Command. ............ 24
Table 5-1. Cable specifications. ............................................................................................................... 34
Table E-1. Configuration Switches 1 – 8................................................................................................ 36
Table E-2. Serial port settings................................................................................................................. 37
Table E-3. Lambda 721 external control commands............................................................................ 38
Table E-4. Lambda 10 Series compatible LED selection commands.................................................. 41
Table E-5. LED selection commands using ASCII digits. .................................................................... 42
Table E-6. Ring Buffer entry values....................................................................................................... 43
Table E-7. Return values while running the Ring Buffer....................................................................
43
Table E-8. ‘M’ Command 1st argument (2nd byte) values for individual LED ON/OFF state bit
encoding................................................................................................................................... 44

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Table E-9. ‘M’ Command 1st argument (2nd byte) values for ON/OFF state bit encoding for all
LEDs and groups. ................................................................................................................... 44

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1. INTRODUCTION
1.1 Introduction
The Lambda 721 is an illumination system designed for the rapid change of wavelength
derived from up to seven light sources of different wavelengths combined into a single beam.
The system comprises of two subsystems: controller with a built-in power supply and an
array of seven LED cube and a liquid light guide or fiber couple. For the most part, this
manual is organized around those subsystems to provide you with ready access to
information, as you need it.
1.1.1 How to Use this Manual
This manual is organized in a manner that is best suited for the typical manner in which the
reader would learn about the system, and then install, operate, and maintain it. Prior to the
table of contents of this manual is a disclaimer and a series of cautionary and warning notes
– it is important that these be read first. This first chapter provides an overall description of
the system, leaving more in-depth technical information towards the end of the chapter. The
next chapter describes how to install the system, followed by one or more chapters that
provide operation instructions, and ending with a chapter on maintenance. Following the
final chapter are several appendices that provide supplemental information. The installation
chapter covers everything related to installation, from unpacking the equipment as received,
through to ensuring correct installation and operation. The operation chapters cover the two
basic types of user interaction with the system: Manual (local) control and external computer
control. Please take the time to read these instructions to assure the safe and proper use of
this instrument.
1.1.2 Technical Support
Sutter Instrument Company at no charge provides unlimited technical support to our
customers. Our technical support staff is available between the hours of 8:00 AM and 5:00
PM (Pacific Time) at [phone]. You may also e-mail your queries to [e-mail].
Furthermore, as this manual is currently under construction, if there are any areas that you
feel should be covered in detail, we would like to hear from you.

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1.2 General Description
Figure 1-1. Lambda 721 indicators, controls, and connectors.
The Lambda 721 is a new concept for combining up to 7 separate LED cubes with different
spectra into a single common output beam. The LED cubes contain the LED, collimating
optics, and a filter. These LED cubes are easily exchanged and installed with no tools
required. Each LED cube is collimated before entering the optical path through the bandpass
filter. The filters for each LED cube also function as mirrors that reflect the collimated beams
from the previous light sources. In the diagram below the optical paths are outlined for each
position including the reflections that occur:
Traditionall y, c ombining more than two light sources required the use of a dichroic ladder.
Dichroic mirrors, which switch from transmission to reflection at one point in the spectrum,
allow the combining of separate light sources, provided that those sources do not have
overlapping wavelengths. The downside of this approach is that light sources cannot be easily
changed. Dichroic ladders also demand careful attention to the order in which the light
sources are introduced into the optical path to avoid having the light blocked by the next
dichroic in line. Typically, additional bandpass filters must be added in front of each light
source before the dichroic, to select the desired range of wavelengths for each source. Each
filter and dichroic used in the ladder decreases the total light output of the system.

LAMBDA 721 OPERATION MANUAL – REV. 1.10C (20210517)
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Figur
e 1-2. Stages of pass-through and reflection from each LED cube light source.
Dichroic ladders also demand careful attention to the order in which the light sources are
introduced into the optical path to avoid having the light blocked by the next dichroic in line.
Typically, additional bandpass filters must be added in front of each light source before the
dichroic, to select the desired range of wavelengths for each source.
1.2.1 Features
Easy to change the spectral output of the light source.
Any LED cube can be placed in any of 7 positions without concern for the order.
Wavelength selection and beam reflection using Semrock® STR Filters.
1.2.2 Common Applications
Fluorescent microscopy
Calcium imaging
FURA
Optogenetics
High speed wavelength selection
The Lambda 721 is designed to keep the size of the beam combiner small and the optical path
short and efficient. Thin-film bandpass filters, such as Semrock’s STR, reflect greater than
90% of out-of-band light. If the band pass of each light source does not overlap, it is possible
to use the filters for both attenuation and
reflection of the light from the other sources. By
arranging the filters and sources into a double
pentagon, we were able to combine seven light
sources in a compact design with lower losses
than previously achievable. As an added benefit,
the last position in the optical train does not
require any filter since no other input reflects

LAMBDA 721 OPERATION MANUAL – REV. 1.10C (20210517)
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from that position. This position can be used with LED’s that have broad spectral output,
such as the 561nm LED, and may allow the excitation of more than single channel of
fluorescence from a single LED. (raw spectra available upon request)
The light from position #1 goes directly to the device output without being reflected. This
position might be preferred for the source with the greatest desired output.
The filter for the seventh light source is not used as a reflective surface and could be used
with LED cubes with broader outputs.
In configurations with fewer than 7 light sources, sources should be filled from lowest to
highest number of reflections to ensure the greatest light output.
This filter is installed at 18° angle-of-incidence to the collimated beam of the LED cube.
This will cause a small shift in the band pass toward shorter wavelengths. While it would
be ideal to have a coating optimized for this application, we have found that stock -STR
filters can be used if you correct for the shift in the band pass when selecting the filters.
The Lambda optical beam combiner is designed for flexibility and expandability. Should
your illumination need change over time a simple configuration change and possibly
additional filters can produce an entirely different output.
Figure 1-3. Lambda 721 LED Spectra Options.

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2. INSTALLATION
2.1 Unpacking
The Lambda 721 and associated hardware comes packed in a single carton. The following is a
list of the components found there. If you believe that any of these components are missing
or show obvious signs of damage from shipping, please contact the f a c t o r y.
Lambda 721 cabinet containing factory-installed power supply, control electronics, seven
light source drivers, front-panel controls, and rear-panel external-control connectors.
Seven LED cube modules with optics and filter for the following wavelength
(nanometers): 340, 365, 385, 410, 440, 460, 480, 480 Narrow Band, 506, 506 Narrow
Band, 530, 561, 590, 617, 630, 660, 740, 850, or 940. Each cube’s optics (mirror & filter)
are especially installed and aligned according to the LED’s wavelength chosen.
Power Cord
3mm Liquid Light Guide with dovetail C-mount extension or SMA fiber optic cable and
adapter
Small Zeiss female dovetail
USB Interface Cable
Manual
One or more additional LED cube assemblies containing different wavelength LEDs (if
ordered)
Microscope adapters (if ordered)
2.2 Getting Started
1. Turn the power switch to the OFF (“0”) position.
2. Plug power cable into the mains outlet.
3. Connect the optical output directly to the microscope via the appropriate adapter or to a
liquid light guide, as appropriate.
Figure 2-1. Light guide installation.
4. Power up the Lambda 721 rocking the power switch (rear panel) upwards in “1” position.
Light guide output
connector

LAMBDA 721 OPERATION MANUAL – REV. 1.10C (20210517)
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Figure 2-2. Rear panel showing power entry module receptacle and switch.
5. Refer to the Operations chapter for instructions on turning on the Lambda 721,
operating the controls on the front panel, and making connections to the rear panel.
WARNING: DO NOT LOOK DIRECTLY INTO THE
LIGHT GUIDE! The output of the light or the light guide should be
directed into the microscope using the appropriate adapters, directed
away from anyone’s eyes, and not directed toward any reflective
surface.
Power input module: Receptacle,
fuse, & switch

LAMBDA 721 OPERATION MANUAL – REV. 1.10C (20210517)
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2.3 Configuration
The Lambda 721 is shipped with the LED cube configuration set as ordered. The unit can be
operated manually and many of its features can be demonstrated without having to make
any TTL input, Ring Buffer strobe, or USB connections. After first exploring the manual
operation of the Lambda 721, you may wish to configure the unit by connecting the TTL
inputs for all seven LED channels, the Ring Buffer strobe input, and USB communications
interface for external computer control. The following figures show the locations of these
connectors on the rear panel of the unit, followed by the location of the configuration
switches and their definitions. It is recommended that operating the unit with the factory-
default settings for the configuration switches, as shown in the table.
Figure 2-1. Rear panel showing connectors for TTL input for all 7 LED channels, Ring Buffer strobe input, and USB for
external control.
DIP Switches
Figure 2-2. Left-side panel and DIP switches.
USB Device
Receptacle
TTL Inputs for
LEDs 1 - 7
Ring Buffer
Strobe Control
1 2 3 4 5 6 7 8
Configuration DIP switches accessible
on right side with small sc
rewdriver
through a
ventilation hole
Front
Rear

LAMBDA 721 OPERATION MANUAL – REV. 1.10C (20210517)
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Table 2-1. Configuration Switches 1 – 8 definitions.
Sw #
Definition
State
Setting
Position
1
TTL level state on power-up
Low*
Off*
Up*
High
On
Down
2
I/O interface return of data from Lambda 10-3 “Get
Controller Type & Config.” and “Get Status” commands
(Ver. 1.26+)
Enabled*
Off*
Up*
Disabled On Down
3
Camera Mode while running the Ring Buffer
Disabled: Stays ON until next
strobe*
Off*
Up*
Enabled: Turns OFF before
next strobe
On Down
4
I/O interface return of current Ring Buffer entry LED
number(s) while Ring Buffer is running
Enabled*
Off*
Up*
Disabled
On
Down
5
Select data (“Baud”) rate for I/O interface
9600 bps*
Off*
Up*
57600 bps
On
Down
6
Reserved
Off*
Up*
7
Reserved
Off*
Up*
8
Allow setting of maximum current for each LED on power
up
Disabled*
Off**
Up*
Enabled
On
Down
* Factory default setting.