tecan sunrise User manual

TECAN
Instructions for Use for
SUNRISE
Microplate Absorbance Reader
Document Part No.: 30086638
2021-07
Document Revision No.: 2.9

2 Instructions for Use for SUNRISE No. 30086638 Rev. No. 2.9 2021-07
Tecan Customer Support
If you have any questions or need technical support for your Tecan product,
contact your local Tecan Customer Support organization. Go to
http://www.tecan.com/ for contact information.
Prior to contacting Tecan for product support, prepare the following information
for the best possible technical support (see name plate):
•Model name of your product
•Serial number (SN) of your product
•Software and software version (if applicable)
•Description of the problem and contact person
•Date and time when the problem occurred
•Steps that you have already taken to correct the problem
•Your contact information (phone number, fax number, e-mail address, etc.)

2021-07 Instructions for Use for SUNRISE No. 30086638 Rev. No. 2.9 3
WARNING
CAREFULLY READ AND FOLLOW THE INSTRUCTIONS PROVIDED
IN THIS PUBLICATION BEFORE OPERATING THE INSTRUMENT.
Notice
Every effort has been made to avoid errors in text and diagrams; however, Tecan
Austria GmbH assumes no responsibility for any errors, which may appear in this
publication.
It is the policy of Tecan Austria GmbH to improve products as new techniques
and components become available. Tecan Austria GmbH therefore reserves the
right to change specifications at any time with appropriate verification, validation,
and approvals.
We would appreciate any comments on this publication.
Manufacturer
Tecan Austria GmbH
Untersbergstr. 1A
A-5082 Grödig, Austria
T: [phone]
F: [phone]
www.tecan.com
E-mail: [e-mail]
Copyright Information
The contents of this publication are the property of Tecan Austria GmbH and are
not to be copied, reproduced or transferred to another person or persons without
prior written permission.
Copyright ©Tecan Austria GmbH
All rights reserved.
Printed in Austria
Declaration for EU Certificate
See the last page of these Instructions for Use.
SUNRISE Microplate Absorbance Reader Intended Use
See chapter 2.2.1 Intended Use
About the Instructions for Use
Original instructions. This publication is intended as Instructions for Use for the
SUNRISE Microplate Absorbance Reader, which is designed to measure the light
absorbance (optical density) of samples in 96 well microplates. It is intended as
reference and instruction for the user.
This document instructs how to:
•Install the instrument
•Operate the instrument
•Clean and maintain the instrument
Every time SUNRISE is mentioned the SUNRISE Microplate Absorbance Reader
is intended.
Warranty
3 Year Warranty
As an expert for microplate instrumentation Tecan proves its commitment to
quality and offers a unique 3 year warranty for your SUNRISE Microplate
Absorbance Reader as a standard. Warranty will be invalid if the instrument is
opened or modified.

4 Instructions for Use for SUNRISE No. 30086638 Rev. No. 2.9 2021-07
Warnings, Cautions and Notes
The following types of notices are used throughout this publication to highlight
important information or to warn the user of potentially dangerous situations:
Note
Gives helpful information.
CAUTION
INDICATES A POSSIBILITY OF INSTRUMENT DAMAGE OR DATA
LOSS IF INSTRUCTIONS ARE NOT FOLLOWED.
WARNING
INDICATES THE POSSIBILITY OF SEVERE PERSONAL INJURY, LOSS
OF LIFE OR EQUIPMENT DAMAGE IF THE INSTRUCTIONS ARE NOT
FOLLOWED.
WARNING
INDICATES THE POSSIBLE PRESENCE OF BIOLOGICALLY
HAZARDOUS MATERIAL. PROPER LABORATORY SAFETY
PRECAUTIONS MUST BE OBSERVED.
WARNING
INDICATES LASER. DO NOT STARE INTO THE BEAM!
ATTENTION
NEGATIVE ENVIRONMENTAL IMPACTS ASSOCIATED WITH THE
TREATMENT OF WEEE
•DO NOT TREAT ELECTRICAL AND ELECTRONIC EQUIPMENT AS
UNSORTED MUNICIPAL WASTE.
•WASTE OF ELECTRICAL AND ELECTRONIC EQUIPMENT MUST BE
COLLECTED SEPARATELY.
FOR CALIFORNIA RESIDENTS ONLY:
WARNING
THIS PRODUCT CAN EXPOSE YOU TO CHEMICALS SUCH AS LEAD
WHICH IS KNOWN TO THE STATE OF CALIFORNIA TO CAUSE
CANCER AND BIRTH DEFECTS OR OTHER REPRODUCTIVE HARM.
FOR MORE INFORMATION GO TO:
WWW.P65WARNINGS.CA.GOV/PRODUCT.

2021-07 Instructions for Use for SUNRISE No. 30086638 Rev. No. 2.9 5
Symbols
Manufacturer
Date of manufacture
CE conformity marking
United Kingdom Conformity Assessed
marking shows that the labeled product is following the applicable regulation in
Great Britain.
Consult instructions for use
Catalogue number
Serial Number
Unique Device Identification
The UDI symbol identifies the data carrier on the label.
WEEE symbol
China RoHS symbol
TÜV SÜD MARK
Biological risks
Laser

6 Instructions for Use for SUNRISE No. 30086638 Rev. No. 2.9 2021-07
Abbreviations
Abbreviation
A
Ampere
Abs.
Absorbance
ADC
Analog digital converter
ASCII
American Standard Code for Information Interchange
ASTM
American Society for Testing and Material
C
Celsius
CE
CE conformity marking
cm
Centimeter
F
Fahrenheit
Hz
Hertz
IVD
In vitro diagnostics
kg
Kilogram
l; L
Liter
LED
Light emitting diode
LIS
Laboratory Information System
MB
Megabyte
ml
Milliliter
nm
Nanometer
OD
Optical Density
RC
Remote control
REF
Reference Number/ Order Number
SN
Serial Number
ST
Standard
TW
Tuneable wavelength
TYPE
Name and type of instrument
USB
Universal serial bus
V
Volt
VA
Volt ampere
VGA
Video Graphics Array
VOLTAGE
Voltage

2021-07 Instructions for Use for SUNRISE No. 30086638 Rev. No. 2.9 7
Table of Contents
1. Safety..................................................................................................................................................9
1.1 Instrument Safety.................................................................................................................................. 9
2. General.............................................................................................................................................11
2.1 Introduction......................................................................................................................................... 11
2.2 Area of Application............................................................................................................................. 13
2.2.1 Intended Use........................................................................................................................ 13
2.2.2 User Profile .......................................................................................................................... 14
2.2.3 Options of SUNRISE............................................................................................................ 14
2.3 Specifications...................................................................................................................................... 16
2.3.1 General ................................................................................................................................ 16
2.3.2 SUNRISE Instrument Configuration with 4Filter Option....................................................... 17
2.3.3 SUNRISE Instrument Configuration with 6Filter Option ....................................................... 17
2.3.4 SUNRISE Instrument Configuration with Tuneable Wavelength Option
(Gradient Filter Carriage) ..................................................................................................... 18
2.3.5 SUNRISE Instrument Configuration with Option Temperature Control................................ 19
2.3.6 Integrated Barcode Reader Option (BCR)
(for SUNRISE with Remote Control Option only)................................................................. 20
2.3.7 Microplates........................................................................................................................... 20
2.3.8 Barcode Labels.................................................................................................................... 21
2.4 Instrument Description....................................................................................................................... 22
2.4.1 Back Panel Connections...................................................................................................... 23
2.4.2 Handling the Microplate ....................................................................................................... 24
2.5 Filter Carriage Description................................................................................................................. 25
2.5.1 4Filter Carriage .................................................................................................................... 25
2.5.2 Gradient Filter Carriage........................................................................................................ 25
2.5.3 6Filter Carriage .................................................................................................................... 26
2.6 Instrument Features............................................................................................................................ 27
2.6.1 Measurement Modes ........................................................................................................... 27
2.6.2 Microplate Shaking............................................................................................................... 28
2.7 Instrument Spare Parts and Tools..................................................................................................... 28
2.8 Software for the SUNRISE Microplate Absorbance Reader............................................................ 29
3. Installation Procedure.....................................................................................................................30
3.1 Introduction......................................................................................................................................... 30
3.2 Unpacking and Inspection ................................................................................................................. 30
3.3 Unpacking Procedure......................................................................................................................... 31
3.4 Power Requirements .......................................................................................................................... 31
3.5 Environmental Requirements ............................................................................................................ 32
3.6 Instrument Installation Procedure..................................................................................................... 32
3.7 Installation of Instrument Control Software ..................................................................................... 33
3.8 Defining the Instrument Settings....................................................................................................... 33
3.8.1 Installation of “SUNRISE Instrument Settings” Software...................................................... 33
3.8.2 Starting the “SUNRISE Instrument Settings”........................................................................ 33
3.8.3 Define Instrument Mode....................................................................................................... 34
3.8.4 Define Filter.......................................................................................................................... 35
3.8.5 Define Measurement Mode.................................................................................................. 36
4. Error Messages and Trouble Shooting.........................................................................................37
4.1 Introduction......................................................................................................................................... 37
4.1.1 Table of Error Messages and Trouble Shooting for SUNRISE Mode................................... 37
4.1.2 Table of Error Messages and Trouble Shooting for SPECTRA Mode.................................. 40
4.2 Definition of ‘Overflow’....................................................................................................................... 40
5. Maintenance & Cleaning.................................................................................................................41
5.1 Introduction......................................................................................................................................... 41
5.2 Filter Replacement.............................................................................................................................. 41
5.2.1 Filter Carriages for 4Filter Option......................................................................................... 41
5.2.2 Gradient Filter Carriages for Tuneable Wavelength Option ................................................. 42
5.2.3 6Filter Carriages for 6Filter Option....................................................................................... 43

8 Instructions for Use for SUNRISE No. 30086638 Rev. No. 2.9 2021-07
5.3 Fuse Replacement...............................................................................................................................44
5.4 Cleaning the Instrument......................................................................................................................45
5.4.1 Cleaning the Device..............................................................................................................45
5.4.2 Liquid Spills...........................................................................................................................45
5.5 Preventive Maintenance Plan for SUNRISE.......................................................................................46
5.5.1 Daily......................................................................................................................................46
5.5.2 Weekly..................................................................................................................................46
5.5.3 Every Six Months..................................................................................................................46
5.5.4 Yearly (Customer or Service Technician) .............................................................................46
5.5.5 Every Four Years (Service Technician Required).................................................................46
5.6 Instrument Disinfection.......................................................................................................................47
5.6.1 Disinfection Solutions............................................................................................................47
5.6.2 Disinfection Procedure..........................................................................................................47
5.7 Disinfection Declaration......................................................................................................................49
5.8 Disposal of Instrument........................................................................................................................50
5.8.1 Introduction...........................................................................................................................50
5.8.2 Disposal of Packing Material.................................................................................................50
5.8.3 Disposal of Operating Material..............................................................................................50
5.8.4 Disposal of the Instrument....................................................................................................51
6. Quality Control................................................................................................................................ 52
6.1 Introduction..........................................................................................................................................52
6.2 Optimizing for Maximum Performance..............................................................................................52
6.2.1 Instrument Location ..............................................................................................................52
6.2.2 Operating Procedure.............................................................................................................52
6.2.3 Self Check Procedure...........................................................................................................53
6.3 Operational Qualification (OQ)...........................................................................................................54
6.3.1 QC PAC 2.............................................................................................................................54
6.3.2 Microplate Test.....................................................................................................................54
6.3.3 High Meniscus Liquids..........................................................................................................56
6.3.4 Instrument Precision with Liquid Samples ............................................................................58
6.3.5 Instrument Linearity with Liquid Samples..............................................................................58
Index .......................................................................................................................................................... 61

1. Safety
2021-07 Instructions for Use for SUNRISE No. 30086638 Rev. No. 2.9 9
1. Safety
1.1 Instrument Safety
1. Always follow basic safety precautions when using this product to reduce the
risk of injury, fire, or electrical shock.
2. Read and understand all information in the Instructions for Use (IFU).
Failure to read, understand, and follow the instructions in this document may
result in damage to the product, injury to operating personnel, or poor
instrument performance. Tecan is not responsible for damage or injuries
resulting from incorrect handling of the device.
3. Observe all WARNING and CAUTION statements in this document.
4. Always disconnect the device from the main power supply prior to cleaning
and disinfection.
5. Never open the instrument’s housing.
6. Observe proper laboratory safety precautions such as wearing protective
clothing (e.g. gloves, lab coat, and safety glasses) and the application of
approved laboratory safety procedures.
CAUTION
IF THE INSTRUCTIONS GIVEN IN THIS PUBLICATION ARE NOT
CORRECTLY FOLLOWED, THE INSTRUMENT MAY BECOME
DAMAGED OR PROCEDURES MAY NOT BE CORRECTLY
PERFORMED AND THE SAFETY OF THE INSTRUMENT CANNOT BE
GUARANTEED.
It is assumed that the instrument operators, because of their vocational
experience, are familiar with the necessary safety precautions for handling
chemicals and bio-hazardous substances.
Adhere to the following laws and guidelines:
•National industrial protection law
•Accident prevention regulations
•Safety data sheets of the reagent manufacturers
WARNING
DEPENDING ON THE APPLICATIONS, PARTS OF THE SUNRISE MAY
COME IN CONTACT WITH BIOHAZARDOUS/INFECTIOUS MATERIAL.
MAKE SURE THAT ONLY QUALIFIED PERSONNEL OPERATE THE
INSTRUMENT. IN CASE OF SERVICE OR WHEN RELOCATING OR
DISPOSING OF THE INSTRUMENT, ALWAYS DISINFECT THE
INSTRUMENT ACCORDING TO THE INSTRUCTIONS GIVEN IN THIS
DOCUMENT.
OBSERVE PROPER LABORATORY SAFETY PRECAUTIONS SUCH
AS WEARING PROTECTIVE CLOTHING WHEN WORKING WITH
POTENTIALLY INFECTIOUS SUBSTANCES.

1. Safety
10 Instructions for Use for SUNRISE No. 30086638 Rev. No. 2.9 2021-07
WARNING
THE INSTRUMENT COMPLIES WITH THE EMISSION AND IMMUNITY
REQUIREMENTS DESCRIBED IN IEC 61326-2-6; HOWEVER, THE
ELECTROMAGNETIC ENVIRONMENT SHOULD BE EVALUATED
PRIOR TO THE OPERATION OF THE INSTRUMENT.
IT IS THE OPERATOR'S RESPONSIBILITY TO ENSURE THAT A
COMPATIBLE ELECTROMAGNETIC ENVIRONMENT FOR THE
INSTRUMENT IS MAINTAINED, SO THAT THE INSTRUMENT
PERFORMS AS INTENDED.
DO NOT OPERATE THE INSTRUMENT IN CLOSE PROXIMITY TO
SOURCES OF STRONG ELECTROMAGNETIC RADIATION (E.G.
UNSHIELDED INTENTIONAL RF SOURCES) AS THIS MAY
INTERFERE WITH THE PROPER FUNCTION OF THE
INSTRUMENT AND MAY ALSO LEAD TO INCORRECT RESULTS.
WARNING
IF THE SUNRISE INSTRUMENT OR THE MAGELLAN SOFTWARE
ARE MODIFIED IN ANY WAY, THE PERFORMANCE OF THE READER
MAY BE NEGATIVELY AFFECTED,
THE WARRANTY WILL NO LONGER BE VALID AND THE
INSTRUMENT WILL NOT BE CE CONFORM.

2. General
2021-07 Instructions for Use for SUNRISE No. 30086638 Rev. No. 2.9 11
2. General
2.1 Introduction
Note
The SUNRISE Microplate Absorbance Reader, equipped with option Remote
Control, is intended for use with external software only.
CAUTION
BEFORE STARTING MEASUREMENTS, MAKE SURE THAT THE
MICROPLATE
POSITION A1 IS INSERTED CORRECTLY.
The SUNRISE instruments are fully automatic, microprocessor controlled readers
designed for professional use, enabling the user to measure the light absorbance
(optical density) of samples in 96 well microplates according to the specifications
described in this publication.
Note
Results obtained using the SUNRISE are influenced by the proper use of
the instrument and microplates, according to the instructions given in this
document, as well as the liquid compounds used (reagents, chemistry). The
instructions for use, for storage and for other manipulations in connection
with samples or reagents must be strictly followed. Taking this fact into
consideration, results must be interpreted carefully.
By reading twelve wells simultaneously, the instrument is able to measure a
microplate in approximately six seconds.
The obtained transmission values are converted into OD values according to the
following formula:
oII
TonTransmissi =
Io= incident light
I= detected light (after the sample)
The absorption (Optical Density) is the logarithm of the reciprocal transmission.
T
1
LogOD =
With an innovative range of options, this versatile microplate reader gives
diagnostic and research laboratories all the features for numerous purposes.
Based on a new design concept EPAC, the excellent optical performance and
high quality of the SUNRISE will guarantee fast, reproducible and accurate
measurements.
The SUNRISE is designed to be integrated into Tecan robotic systems.
The SUNRISE Microplate Absorbance Reader is an optical 96-well microplate
reader for the measurement of absorbance and turbidimetry of samples from the
biological or non-biological origin according to the specifications described in this
document, intended for professional use only.

2. General
12 Instructions for Use for SUNRISE No. 30086638 Rev. No. 2.9 2021-07
The SUNRISE Microplate Absorbance Reader is available with a range of options
such as tuneable wavelength, temperature control and barcode scanner.
Magellan is a reader control and data reduction software package, and also can
be used for concentration and titer determination.
Note
It is important to note that the proper installation of the instrument and
Magellan software alone will not ensure regulatory compliance. A range
of policies and standard operating procedures according to local
regulations must also be established.
Make sure the instrument and the software are suitable for use with the reagents,
chemicals and microplates that are being used (see 6.2 Optimizing for Maximum
Performance and 6.3 Operational Qualification).
CAUTION
IF THE INSTRUCTIONS GIVEN IN THIS PUBLICATION ARE NOT
CORRECTLY FOLLOWED,
THE INSTRUMENT MAY BECOME DAMAGED OR PROCEDURES MAY
NOT BE CORRECTLY PERFORMED AND THE SAFETY OF THE
INSTRUMENT CANNOT BE GUARANTEED.
Note
For more information about the operating instructions,
see Magellan or XFluor4 Instructions for Use.
WARNING
CAREFULLY READ AND UNDERSTAND ALL INFORMATION IN THIS
DOCUMENT. FAILURE TO READ, UNDERSTAND AND FOLLOW THE
INSTRUCTIONS IN THIS PUBLICATION MAY RESULT IN DAMAGE TO
THE PRODUCT, INJURY TO OPERATING PERSONNEL OR POOR
INSTRUMENT PERFORMANCE.

2. General
2021-07 Instructions for Use for SUNRISE No. 30086638 Rev. No. 2.9 13
2.2 Area of Application
2.2.1 Intended Use
The SUNRISE instrument is a 96-well absorbance reader for the measurement of
light absorbance (optical density) of liquid media. The instrument is intended to
be used primarily in in-vitro diagnostic analysis of samples from the human body
to obtain information on physiological and pathological states.
For applications in human clinical diagnostic uses, only the Magellan software is
intended for the use with the instrument. Software and instrument have been
validated for measurement and for the evaluation of qualitative and quantitative
Enzyme-linked Immunosorbent Assays (ELISA) according to the scheduled
diagnostic parameters and instrument specifications; they are therefore for
professional use in in-vitro diagnostics.
Note
If the SUNRISE or the Magellan software is modified in any way, the
warranty will no longer be valid and the instrument will lose regulatory
conformity.
Note
Results obtained using the SUNRISE are influenced by the proper use of
the instrument and microplates, according to the instructions given in
this document, as well as the liquid compounds used (reagents,
chemistry). The instructions for use, storage, and applications involving
samples or reagents must be followed strictly. Results must therefore be
interpreted carefully.

2. General
14 Instructions for Use for SUNRISE No. 30086638 Rev. No. 2.9 2021-07
2.2.2 User Profile
Professional User - Administrator Level
The administrator is a person who has suitable technical training and
corresponding skills and experiences. If the product is used as intended, the
person is able to recognize and avoid dangers.
The administrator has extensive skills and is able to instruct the end user or the
routine user in assay protocols in connection with a Tecan product within the
bounds of the intended use.
Computer application skills and good English skills are required.
End User or Routine User
The end user or routine user is a person who has suitable technical training and
corresponding skills and experiences. If the product is used as intended, the
person is able to recognize and avoid dangers.
Computer application skills and good language skills for the respective national
language at the installation site and English are required.
Service Technician
The service technician is a person who has suitable technical training and
corresponding skills and experiences. If the product needs to be serviced or
maintained, the person is able to recognize and avoid dangers.
Computer application skills and good English skills are required.
Note
Training dates, their duration and frequency are available at your
customer support.
Address and phone number can be found in the web:
http://www.tecan.com/customersupport
2.2.3 Options of SUNRISE
The SUNRISE is a modular system, so you can create your own tailor-made
instrument that meets exactly your needs. Options such as free wavelength
selection, temperature control and an integrated barcode scanner are available.

2. General
2021-07 Instructions for Use for SUNRISE No. 30086638 Rev. No. 2.9 15
SUNRISE Options
Option Level
Description
User Interface
Remote control (RC)
LIS (handheld barcode scanner plus ASTM
export)
This option is not used for clinical
diagnostics in China.
Barcode (BCR), option for SUNRISE
with remote control (RC)
This option is not used for clinical
diagnostics in China.
Optics
4-Filter (ST)
6-Filter (6F)
Tuneable wavelength (TW)
This option is not used for
clinical diagnostics in China.
Options
Temperature control (TC)

2. General
16 Instructions for Use for SUNRISE No. 30086638 Rev. No. 2.9 2021-07
2.3 Specifications
The tables below list the specifications for the instruments in SUNRISE mode.
2.3.1 General
For all instrument options:
PARAMETERS
CHARACTERISTICS
Main power input
100 - 120 & 220 - 240 V, 50/60 Hz
(Auto sensing)
Consumption
Operational mode: max. 110 VA
Fuse rating
2 x F 2.0 A / 250 V (Fast Blow)
Outside dimensions
Width: 28.5 cm (11.22 inch)
Depth: 34.0 cm (13.39 inch)
Height: 14.5 cm (5.71 inch)
Weight
Max. (incl. all options)
8.6 kg
Ambient temperature:
Operation
15°C to 35°C (59°F to 95°F)
Storage
-20°C to 60°C (-4°F to 140°F)
Relative humidity:
Operation
20 % to 90 %
Storage
5 % to 95 %
Over voltage category
II
Pollution degree
2
Method of disposal
Contaminated waste
Environment
See 3.5 Environmental Requirements for more
information.
Stability:
Normal measurements
After 15 minutes warm up time
max. +/- 0.001 OD

2. General
2021-07 Instructions for Use for SUNRISE No. 30086638 Rev. No. 2.9 17
2.3.2 SUNRISE Instrument Configuration with 4Filter Option
PARAMETERS
CHARACTERISTICS
Measurement time:
dual wavelength
single wavelength
8 seconds
6 seconds
Wavelength range:
Standard filter
340 - 750 nm
Measurement range:
340 - 399 nm
400 - 750 nm
0 - 3.000 OD
0 - 4.000 OD
Resolution:
0.001 OD
Accuracy:
(492 nm) 0.000 - 2.000 OD
(492 nm) 2.000 - 3.000 OD
< (1.0 % + 0.010 OD) *
< (1.5 % + 0.010 OD) *
Precision:
(492 nm) 0.000 - 2.000 OD
(492 nm) 2.000 - 3.000 OD
< (0.5 % + 0.005 OD) *
< (1.0 % + 0.005 OD) *
Linearity:
(340 - 399 nm) 0.000 - 2.000 OD
(400 - 750 nm) 0.000 - 2.000 OD
2.000 - 3.000 OD
< 2 %
< 1 %
< 1.5 %
(340 - 399 nm) 0.000 - 2.000 OD
(400 - 750 nm) 0.000 - 2.000 OD
(400 - 750 nm) 2.000 - 3.000 OD
R² >= 0.999
R² >= 0.999
R² >= 0.999
* better than x % of measurement value plus corresponding OD value.
Note: All deviations from the measurement value are meant in positive and negative direction.
Wavelength selection:
Standard filter
Narrow band interference filters. Up to
four filters can be mounted in a filter
carriage. The instrument can use up to
eight different filter carriages.
Filter wavelength accuracy
Central wavelength +/- 2 nm
Filter bandwidth
At 50% transmission:
10 +/- 2 nm
Light source:
Halogen lamp 20 W
All connected devices must be approved and listed as per IEC 60950-1
Information Technology Equipment –Safety and equivalent local standards.
Computer interface:
Serial RS 232 C
300 - 38,400 baud
2.3.3 SUNRISE Instrument Configuration with 6Filter Option
See 2.3.2 SUNRISE Instrument Configuration with 4Filter Option .

2. General
18 Instructions for Use for SUNRISE No. 30086638 Rev. No. 2.9 2021-07
2.3.4 SUNRISE Instrument Configuration with Tuneable
Wavelength Option (Gradient Filter Carriage)
PARAMETERS
CHARACTERISTICS
Measurement time:
Dual wavelength
Single wavelength
16 seconds
8 seconds
Wavelength range:
Gradient filter
Standard filter
400 - 700 nm
340 - 399 nm & 700 - 750 nm
Measurement range:
340 - 399 nm
400 - 750 nm
0 - 3.000 OD
0 - 4.000 OD
Resolution:
0.001 OD
Accuracy:
(492 nm) 0.000 - 2.000 OD
< (1.5 % + 0.010 OD) *
Precision:
(492 nm) 0.000 - 2.500 OD
< (1.0 % + 0.005 OD) *
Linearity:
(492 nm) 0.000 - 2.500 OD
< 2 %, R² >= 0.999
Wavelength selection:
Gradient filter
Special gradient filter any wavelength
between 400 and 700 nm in 1 nm steps.
The instrument can use up to eight
different filter carriages
* better than x % of measurement value plus corresponding OD value
Note: All deviations from the measurement value are meant in positive and negative direction.
Filter wavelength accuracy
Central wavelength +/- 2 nm
Filter bandwidth
At 50% transmission:
450 nm
550 nm
650 nm
8.5 - 16 nm
10 - 15 nm
10 - 18 nm
Light source:
Halogen lamp 20 W
All connected devices must be approved and listed as per IEC 60950-1
Information Technology Equipment –Safety and equivalent local standards.
Computer interface:
Serial RS 232 C
300 - 38,400 baud

2. General
2021-07 Instructions for Use for SUNRISE No. 30086638 Rev. No. 2.9 19
2.3.5 SUNRISE Instrument Configuration with Option
Temperature Control
Peltier-based temperature control.
PARAMETERS
CHARACTERISTICS
Temperature range:
Room temperature up to 42°C increments of 0.1°C
Accuracy
typical +/- 0.2°C (max. +/- 0.5°C)
Preheating time
30 min
The SUNRISE specification of the temperature control unit (typical accuracy +/-
0.2°C) applies if everything (reader, microplate, reagents, samples) has already
target temperature.
There is no specification for the heating up period and we are aware that within
this period not all wells are heated up equally. This effect will be seen especially
when reagents are not preheated.
During preheating the plate transport with a 96 well microplate loaded has to be
inside of the instrument. Only in this way a homogenous temperature is received
in the instrument. The microplate must not be the same as the one used for
measurement but should be clean to keep influences on the following
measurement low.
Every time the plate transport is moved it will take up to 1 min to reach again a
homogenous temperature inside of the instrument.
Bear in mind that if non-preheated microplate and/or reagents are added,
temperature control will take a certain time depending on the temperature
difference between microplates.
We recommend a longer measurement interval (e.g. 1 min) to increase
homogenous temperature distribution. Keep the difference between target
temperature and the temperature of microplate with reagents and samples lower
than 20 °C (68 °F).

2. General
20 Instructions for Use for SUNRISE No. 30086638 Rev. No. 2.9 2021-07
2.3.6 Integrated Barcode Reader Option (BCR) (for SUNRISE
with Remote Control Option only)
WARNING
LASER RADIATION –DO NOT STARE INTO THE BEAM!
CLASS II / 2 LASER PRODUCT.
The Class II / 2 Laser Scanner corresponds to the following norms:
•DIN EN 60825-1 : 2007
•CDRH 21 CFR 1040.10
2.3.7 Microplates
Only 96-well format microplates without lid (v-shaped, flat and round including
strip-wells) with transparent bottom can be used with the SUNRISE Microplate
Absorbance Reader.
PARAMETERS
CHARACTERISTICS
Max. overall plate height
14.35 mm +/- 0.76 mm
(0.5650 inches +/- 0.0299 inches)
Diameter of wells
7.0 mm (0.276 inches)
Pitch size (center to center)
9.0 mm (0.3543 inches)
Bottom shape
V-shaped, Flat and Round bottom
The barcode sticker has to be positioned on the right hand side of the plate,
7 mm from the front-right edge. The barcode may be up to 48 mm long.