tecan M1000 Installation and operating instructions

m1000™ Operating Manual Addendum, 392813, en, V1.1
m1000™ Operating Manual Addendum
for the Advanced Operator

© 2003, Tecan Schweiz AG, Switzerland, all rights reserved
Information contained in this document is subject to change without notice.
Pictorial Disclaimer
The screens and figures contained in this manual are for information and illustration purposes only. Abbott Laboratories makes no repre-
sentations or warranties about the accuracy and reliability of the information on the screens and figures in this manual; their information is
not to be used for clinical or maintenance evaluation.
Manufacturer Address
Tecan Schweiz AG
Seestrasse 103
CH-8708 Männedorf
Switzerland
Distributor Address
Abbott Laboratories
Abbott Park, IL 60064
USA
Document Status Sheet
Title: m1000™ Operating Manual Addendum for the Advanced Operator
ID: 392813
Version Revision Issue Major changes
1 0 may 2003 First issue
1 1 LiHa/RoMa Calibration Procedure updates
New Procedure: Move Plate Test.

m1000™ Operating Manual Addendum, 392813, en, V1.1 iii
Table of Contents
Table of Contents
m1000™ Operating Manual Addendum .......................................................1
Notices and Symbols .....................................................................................2
NAT Admin Software ......................................................................................4
Start NAT Admin .............................................................................................5
NAT Worktable ................................................................................................6
NAT Options ...................................................................................................7
Database Maintenance ...................................................................................7
Restore Database ...........................................................................................8
Temperature Check ........................................................................................9
NAT Teach Worktable Racks and Locations .............................................10
Calibrate the LiHa .........................................................................................11
Calibrating the DiTi rack carriers for the LiHa ...........................................15
Removing the DiTi Reference Tip ...............................................................17
Calibrate the RoMa .......................................................................................18
Calibrating the DiTi rack carriers for the RoMa .........................................20
Calibrating the DiTi rack shelves for the RoMa .........................................22
Fine-tuning the DiTi rack carriers for the LiHa ..........................................23
Move Plate Test ............................................................................................25
Disconnect from the Instrument .................................................................25
GENESIS Setup & Service Procedures ......................................................26
NAT MBS Worktable .....................................................................................27

iv m1000™ Operating Manual Addendum, 392813, en, V1.1
Table of Contents
Initialise Instrument ......................................................................................29
Dilutor Tool ....................................................................................................30
Door Lock Check ..........................................................................................32
FaWa Test ......................................................................................................36
Liquid Detection Test ...................................................................................40
Lower DiTi Eject Test ...................................................................................43
PosID Test .....................................................................................................46

m1000™ Operating Manual Addendum, 392813, en, V1.1 1
m1000™ Operating Manual Addendum
Read this information prior to using the NAT Admin or the GENESIS Setup &
Service software features. This information should be used in conjunction with
the m1000™ Operating Manual.
This information is issued by Abbott Diagnostics Division to provide you with
advanced m1000™ information.
Intended Use of Operating Manual Addendum:
This manual supports the operator of the m1000™ with instrument calibration
and troubleshooting procedures.
This manual does not enable the operator to generate user-defined protocols.
It is strongly recommended that operators contact Abbott Laboratories for
assistance with any protocol or process step change.
Categories include:
NAT Admin
NAT Worktable
NAT Options
Database Maintenance
Temperature Check
NAT Teach Worktable
GENESIS Setup & Service procedures
NAT MBS Worktable
Initialise Instrument
Dilutor Tool
Door Lock Check
FaWa Test
Liquid Detection Test
Lower DiTi Eject Test
PosID Test
User Qualification
The laboratory personnel must be qualified and trained to operate the m1000™.
The operators must fulfill the following qualifications to perform the procedures
described in this Addendum:
They must have a thorough knowledge of the applications run on the system.
They must be familiar with the Good Laboratory Practice guidelines or
equivalent guidelines.
They must have read and understood the instructions in the m1000™
Operating Manual and this Addendum.
Note: Abbott offers onsite training for the primary operator. Abbott recommends
that the primary operator attend this primary operator training course.

2m1000™ Operating Manual Addendum, 392813, en, V1.1
Notices and
Symbols
Warning Notices Used in this Manual
The symbols used for safety-related notices have the following significance:
Warning Symbols
WARNINGS appear as follows:
WARNING
Generally, the triangular warning symbol indicates the possibility of personal injury
or even loss of life if the instructions are not followed.
Whenever possible, the symbol indicates the hazard a person is exposed to more
specifically. The symbols used in this Operating Manual Addendum have the
following significance:
WARNING
Potentially infectious material
Indicates an area where the operator may be exposed to infectious material.
WARNING
Automatically moving parts.
Injuries (crushing, piercing) possible, if the safety panels are not in place.
Before starting the m1000™, make sure that the safety door is closed.
Never operate the instrument without panels on and door closed.
WARNING
Laser Hazard
Identifies a Class II laser product that contains an embedded low power
visible Class II laser diode barcode scanner. Warns against direct viewing into
barcode laser beam or reflections from the beam.
WARNING
Hot Surface Hazard: Identifies an area where the operator may be exposed to hot
surfaces, such as heater zone 1 and heater zone 2.

m1000™ Operating Manual Addendum, 392813, en, V1.1 3
ATTENTION Symbols
ATTENTIONS appear as follows:
ATTENTION
With the general “Read This!” symbol, ATTENTION indicates the possibility of
equipment damage, malfunctions, or incorrect process results if instructions are
not followed.

4m1000™ Operating Manual Addendum, 392813, en, V1.1
NAT Admin Software
NAT Admin
Software
The NAT Admin software allows you to configure the m1000™ with information to
meet your site’s specific requirements.
WARNING
The Abbott 1 ml Protocol v1.0 cannot be edited.
The use of the Abbott sample preparation kit and the provided Generic 1 ml
Protocol will allow an operator successful RNA separation. The Generic 1 ml
Protocol processes all specimens and controls as samples. Because the NAT
software does not check dependencies of process steps, instrument damage
and/or poor specimen yield and purity may result from any protocol or process
step change. It is strongly recommended that users contact Abbott Laboratories
for assistance with any protocol or process step change. Technical Support may
be limited for any changes to the Generic 1 ml Protocol not recommended by
Abbott Laboratories. The user has sole responsibility to verify and/or validate any
user-defined protocols.
WARNING
Automatically moving parts.
Injuries (crushing, piercing) possible, if the safety panels are not in place.
Before starting the m1000™, make sure that the safety door is closed.
Never operate the instrument without panels on and door closed.
WARNING
Potentially infectious
Instrument parts may be contaminated with potentially infectious materials.
Follow basic biohazard precautions
Wear appropriate personal protective equipment, such as gloves, lab coats
and protective eye wear
WARNING
The operator may be exposed to hazardous chemicals when handling sample
preparation reagents. The exposure to hazardous chemicals is minimized by
following instructions provided in produce-specific labels and product-specific
Material Safety Data Sheets (MSDS). Wear appropriate personal protective
equipment, such as gloves, lab coats, and protective eye wear.

m1000™ Operating Manual Addendum, 392813, en, V1.1 5
Start NAT
Admin
The NAT Wizard must be closed to start the NAT Admin.
Select the NAT Admin icon.
The NAT Admin Login screen is displayed.
Enter User Name and Password (case sensitive) and select <OK>.
The NAT Admin main screen is displayed.

6m1000™ Operating Manual Addendum, 392813, en, V1.1
NAT Worktable The following figure shows the m1000™ worktable locations used in NAT Admin
software:
Note: Worktable locations are fixed and cannot be changed.
Fig. 1 NAT Admin Worktable
A
B
C
KN
LO
P
M
GH
IJ
1
2
3
7
8
QRSTUVWX
9
D
F
E
A
Control Loc
BInput Loc
COutput Loc
D System Fluid
EDiTi Waste
FLiquid Waste
G1 ml Subsystem Loc Heater Zone 1
H1 ml Subsystem Loc Heater Zone 2
I1 ml Subsystem Loc RT
J1 ml Subsystem Loc Mag Low
KPlate Carrier 1, Loc 1
LPlate Carrier 1, Loc 2
MPlate Carrier 1, Loc 3
NPlate Carrier 2, Loc 1
OPlate Carrier 2, Loc 2
PPlate Carrier 2, Loc 3
QShelf Loc 1
RShelf Loc 2
SShelf Loc 3
TShelf Loc 4
UShelf Loc 5
VShelf Loc 6
WShelf Loc 7
X
Shelf Loc 8
1Trough Loc 1
2Trough Loc 2
3Trough Loc 3
7Trough Loc 7
8Trough Loc 8
9Trough Loc 9

m1000™ Operating Manual Addendum, 392813, en, V1.1 7
NAT Options The Options screen allows the operator to configure printers, directories, log
durations, and barcode settings.
Accessing the Options Page:
1Select the NAT Admin icon.
2Enter User Name and Password. Select <OK>.
3The NAT Admin main screen is displayed.
4Select Options from the NAT Admin main screen.
5Select Set NAT Options.
Note: Barcode length for Code 2/5 must be an even number of characters. The
barcode length configured should include the check digit and extra character, if
necessary, for an even number of characters.
Database
Maintenance
Database Maintenance is performed daily at 01:00 a.m. in the form of a daily
backup. In the case of a database corruption, a previous database can be
restored.
Field Description
Default Printer Select the default printer for report printing.
Import Directory Select default directory from which to import files
Export Directory Select default directory to export files.
Days to save log entries Default is 100
Barcode Settings
Code 2/5 Interleaved*
Codabar
Configure the appropriate barcode length
Select 2 to 30
Select 1 to 31
*

8m1000™ Operating Manual Addendum, 392813, en, V1.1
The backup files will be assigned a name based on the date of backup.
For example,
NATMBS_db_200304010100.BAK
NATMBS will always precede the name
20030401 is the backup date: Year/Month/Date
0100 is the backup time
BAK is the file extension
Restore
Database
1Select the NAT Admin icon.
2Enter User Name and Password (case sensitive) and select <OK>.
3The NAT Admin main screen is displayed.
4Select Tools from the NAT Admin main menu.
5Select Database Maintenance.
6Select the file to restore.
7Select Restore Now.
8Select Close to exit to the NAT Admin main menu.

m1000™ Operating Manual Addendum, 392813, en, V1.1 9
Temperature
Check
The temperature check for the 1 ml subsystem should be performed according to
your laboratory procedures or as directed for troubleshooting. Refer to the
m1000™ Operating Manual, Chapter 8, Troubleshooting, for additional
assistance.
WARNING
Potentially infectious
Instrument parts may be contaminated with potentially infectious materials.
Follow basic biohazard precautions
Wear appropriate personal protective equipment, such as gloves, lab coats
and protective eye wear
WARNING
Hot Surface Hazard: Identifies an area where the operator may be exposed to hot
surfaces, such as Heater Zone 1 and Heater Zone 2.
Perform the following steps:
1Power on the m1000™, if necessary.
2Select the NAT Wizard icon from the desktop screen. The Main menu is
displayed.
3From the Main Menu, select Select Protocol.
4The Authorization dialog window is displayed. Enter your User Name and
password and select >to proceed to the next screen.
5Select the Abbott 1 ml Protocol v1.0 or the Generic 1 ml Protocol v1.0 to be
run.
6Place one reaction vessel in the 1 ml subsystem carrier (any location). Add
approximately 1 ml of water, or enough to cover the base of the thermometer.
Place the 1 ml subsystem carrier into heater zone 1.
7Select >to proceed.
8The operator is prompted to initiate the Pre-heat protocol to allow heater
zone 1 to reach correct temperature. Select <OK>.
9Once heater zone 1 reaches the correct operating temperature, the operator
is prompted Heater Zone 1 Achieved Operating Temperature. Select
<OK>.
10 Insert a thermometer into the reaction vessel in heater zone 1. Allow to
equilibrate to 50°C (± 2.5°C). Verify this temperature is 50°C (± 2.5°C).
Note: DO NOT place the thermometer into the reaction vessel until the Scan
Rack screen displays.
11 Move the 1 ml subsystem carrier to heater zone 2. Allow the thermometer to
equilibrate to 75°C (± 2.5°C). Verify this temperature is 75°C (± 2.5°C).
12 If the temperature for heater zone 1 and heater zone 2 are not within the
specified range, repeat this procedure. Do not run a sample preparation
protocol until the temperature for both heater zones are correct. Refer to the
m1000™ Operating Manual, Chapter 8, Troubleshooting, for additional
assistance.
13 Remove the thermometer.
14 Select <twice to return to the Main Menu.

10 m1000™ Operating Manual Addendum, 392813, en, V1.1
NAT Teach
Worktable
Racks and
Locations
The liquid handling arm (LiHa) and robotic manipulator arm (RoMa) must be
calibrated with the GENESIS Setup & Service software by an Abbott Field
Service Engineer. In addition, once the GENESIS Setup & Service Worktable
calibrations are performed, the NAT software requires calibration. This can be
performed using the Teach Worktable Racks and Locations screen.
In addition, the vertical and horizontal position for the LiHa and RoMa for carriers
and racks on the m1000™ worktable must be determined by using the NAT
Admin Teach Worktable Racks and Locations procedures.
General Information
WARNING
Automatically moving parts.
Injuries (crushing, piercing) possible, if the safety panels are not in place.
Before starting the m1000™, make sure that the safety door is closed.
Never operate the instrument without panels on and door closed.
WARNING
Potentially infectious
Instrument parts may be contaminated with potentially infectious materials.
Follow basic biohazard precautions
Wear appropriate personal protective equipment, such as gloves, lab coats
and protective eye wear
WARNING
All carriers and racks must be removed from the m1000™ worktable, including the
sample and control racks, reagent trough carriers, 1 ml subsystem carrier, DiTi
racks, waste station chute, and 1.5 ml output tube carriers. Failure to remove
these instrument accessories could result in crashing and damage to the LiHa,
calibration reference tip, and/or the RoMa.
To access the Teach Worktable Racks and Locations screen:
1Select the NAT Admin icon.
2Enter User Name and Password and select <OK> to continue. The NAT
Admin screen is displayed.
3Select File from the NAT Admin main screen.
4Select Teach Worktable Locations.
5The Teach Worktable Racks and Locations screen is displayed.

m1000™ Operating Manual Addendum, 392813, en, V1.1 11
7The operator is prompted to remove the sample and control racks, reagent
trough carriers, 1 ml subsystem carrier, DiTi racks, waste station chute, and
1.5 ml output tube carriers. Select <OK>.
8Once the instrument is connected, the Connect to the Instrument button will
display Disconnect from the instrument.
9Close the Safety door.
10 Select Initialise all Devices. The LiHa, RoMa, and PosID will move to the
Home position and the Instrument status button will change to Instrument
Status In Init during initialisation.
11 Once Initialization is complete, the Instrument status button will change to
Instrument Status Connected.
Calibrate the
LiHa
Calibrating the 1 ml subsystem for the LiHa
During this step, the LiHa is calibrated to correctly aspirate and dispense
specimens, controls, and reagents into the reaction vessels. This calibration will
include X, Y, and Z direction.
Fig. 2 Teach worktable locations
6Select Connect to the Instrument.
Connect
to the
Instrument

12 m1000™ Operating Manual Addendum, 392813, en, V1.1
The target for the 1 ml subsystem is the small indention in the center of each
zone. The reference tip should just touch the target in the X and Y direction and
the tip of the reference tip must touch or just be level with the surface of each
zone.
1Under Calibrate LiHa, select Mount Reference Tip. The DiTi channels will
separate, allowing easy access to install the reference tip to the third DiTi
channel.
2The operator is prompted to mount the reference tip to DiTi #3.
Fig. 3 Mounting the Reference Tip
The LiHa can move in three different
directions:
X: arm movement left and right
Y: tip movement and spacing front and rear
Z: range for tip movement up and down.

m1000™ Operating Manual Addendum, 392813, en, V1.1 13
3Touch the workstation before touching DiTi cones to discharge static electricity
from your body.
4Add the reference tip to DiTi #3.
– Using the DiTi cone wrench, turn the DiTi cone #3 clockwise to loosen and
remove.
– Remove the black tip ejector tube.
– Disconnect the tubing extension from the tubing.
– Unscrew the stainless steel adapter cylinder.
– Screw the reference tip on the tip adapter and tighten.
– Set aside the DiTi cone, tubing extension, and adapter cylinder.
5Select <OK> once the reference tip is mounted.
6Close Safety door.
7The operator is prompted that the LiHa will move to its Home position. Select
<OK>.
8Select a calibration point from the list box icon located under the Current
Rack field. The following 1 ml subsystem positions need to be calibrated with
the LiHa using the NAT software:
– 1 ml Heater Zone 1 Calibration Point
– 1 ml Heater Zone 2 Calibration Point
– 1 ml Mag Calibration Point
– 1 ml RT Calibration Point
– 1 ml Mag Rest Calibration Point
A. Tip ejector tube (black, grooved end up)
B. Tubing extension
C. DiTi cone

14 m1000™ Operating Manual Addendum, 392813, en, V1.1
9Select Travel Home to home the LiHa.
10 Select Travel With the LiHa to Location on Carrier. The LiHa will move to
the selected position.
11 Select a Travel increment of 1, 10, or 100, with 1 being the smallest travel
increment and 100 being the largest travel increment.
– The target for the 1 ml subsystem is the small indention in the center of
each zone. The reference tip should just touch the target in the X and Y
direction and the tip of the reference tip must touch or be level with the
surface of each zone. A piece of paper may be used to ensure a flat
surface.
– Adjust the reference tip to the X and Y positions first, and then adjust for
the Z direction.
– Use the "<" or ">" buttons beside X/Y to move the LiHa in the X direction.
– Use the "^" or "v" buttons beside X/Y to move the LiHa in the Y direction.
– Use the "^" or "v" buttons under Z to move the LiHa in the Z direction.
– Once the reference tip is close to the target, select a target increment of 1
to adjust the reference tip as close to the target as possible.
– Once the reference tip is touching the surface of the zone, select Save
LiHa calibration.
12 The position should be verified. Select Travel Home to home the LiHa. Next,
select Travel with the LiHa to Location on Carrier. The LiHa returns to the
new position. If the position is incorrect, repeat steps 8 through 11. If the
position is correct, select Travel Home.
Fig. 4 Calibrate LiHa
Fig. 5 1 ml RT Calibration point

m1000™ Operating Manual Addendum, 392813, en, V1.1 15
13 Once the X, Y, and Z direction are calibrated and saved for one location, then
repeat steps 8 through 12 for each remaining location to be calibrated.
14 The 1 ml Mag Rest Calibration Point is calibrated as described below:
Place the 1 ml subsystem carrier (without cover) in the Mag Rest position,
allowing the carrier to be in the raised position. Close the Safety door.
Select 1 ml Mag Rest Calibration Point.
The operator is prompted to only set the Z direction for the 1 ml Mag Rest
Calibration Point. Select <OK>.
Select Travel with the LiHa to Location on Carrier. To avoid setting the
reference tip in the groove, move the LiHa to the right (X adjustment) by
20 steps. Adjust the reference tip such that the tip is level with or slightly
above the 1 ml subsystem. A piece of paper may be used to adjust the
Z-height. When the Z direction has been calibrated, move the LiHa back to the
left (X adjustment) by 20 steps.
Once the reference tip is even with the main surface of the carrier, select Save
LiHa calibration.
The position should be verified. Select Travel Home to home the LiHa. Next,
select Travel with the LiHa to Location on Carrier.The LiHa returns to the
new position.
If the position is incorrect, repeat the above steps.
If the position is correct, select Travel Home.
Remove the 1 ml subsystem carrier from the worktable.
Calibrating the
DiTi rack
carriers for the
LiHa
During this step, the LiHa is calibrated to correctly pick up disposable tips (DiTis)
used for aspiration and dispense and delivery of DiTis to the DiTi Reuse rack. This
calibration will include X, Y, and Z direction.
Reference tip
level with main
surface.

16 m1000™ Operating Manual Addendum, 392813, en, V1.1
The target for the DiTi carrier racks is the left, rear inside corner. The reference tip
should just touch the inside corner in the X and Y direction, without the reference
tip deflecting in either direction. The tip of the reference tip must touch or just be
level with the surface of the DiTi rack carrier.
1Select a carrier from the list box icon located under the Current Rack field.
The following positions on the worktable need to be calibrated with the LiHa
using the NAT software:
2Remove the DiTi racks and DiTi reuse racks.
Note: Before calibrating the LiHa, make certain the DiTi rack carrier (plate carrier)
is properly seated on the worktable.
3Close the Safety door.
4Select Travel With the LiHa to Location on Carrier. The LiHa will move to
the selected position.
5Select a Travel increment of 1, 10, or 100, with 1 being the smallest travel
increment and 100 being the largest travel increment.
Fig. 6 Calibrate LiHa
A
B
C
D
E
F
APlate Carrier 1 Loc 1
BPlate Carrier 1 Loc 2
CPlate Carrier 1 Loc 3
DPlate Carrier 2 Loc 1
EPlate Carrier 2 Loc 2
FPlate Carrier 2 Loc 3