Thermo Scientific TSQ 8000 Evo User manual

TSQ 8000 Evo
Mass Spectrometer
Preinstallation Requirements Guide
1R120586-0001 Revision C December 2015

© 2015 Thermo Fisher Scientific Inc. All rights reserved.
TSQ, ISQ, TRACE, TriPlus, and TraceFinder are trademarks, and Xcalibur is a registered trademark of
Thermo Fisher Scientific in the United States.
The following are registered trademarks in the United States and other countries: Microsoft, Windows, Office,
Skydrive, and Excel are registered trademarks of Microsoft. Swagelok® is a registered trademark of Swagelok
Company.
All other trademarks are the property of Thermo Fisher Scientific and its subsidiaries.
Thermo Fisher Scientific Inc. provides this document to its customers with a product purchase to use in the
product operation. This document is copyright protected and any reproduction of the whole or any part of this
document is strictly prohibited, except with the written authorization of Thermo Fisher Scientific Inc.
The contents of this document are subject to change without notice. All technical information in this
document is for reference purposes only. System configurations and specifications in this document supersede
all previous information received by the purchaser.
Thermo Fisher Scientific Inc. makes no representations that this document is complete, accurate or error-
free and assumes no responsibility and will not be liable for any errors, omissions, damage or loss that might
result from any use of this document, even if the information in the document is followed properly.
This document is not part of any sales contract between Thermo Fisher Scientific Inc. and a purchaser. This
document shall in no way govern or modify any Terms and Conditions of Sale, which Terms and Conditions of
Sale shall govern all conflicting information between the two documents.
Release history: Revision A, July 2014; Revision B, March 2015; Revision C, December 2015.
For Research Use Only. Not for use in diagnostic procedures.

IMPORTANT PREINSTALLATION INFORMATION... PLEASE READ
TSQ 8000 Evo Installation Request Form
Dear Customer:
Read the TSQ 8000 Evo Preinstallation Requirements Guide, and then print and complete the following installation request form.
After all items on the form are fulfilled, sign and date the form. Then, scan and email, mail, or fax this form to your local
Thermo Fisher Scientific sales/service office. The address and fax number for your local office are located on the following pages.
Print your name, company name, and company address clearly below:
Name ________________________________________________________
Company _____________________________________________________ Telephone____________________
Address ___________________________________________________________________________________
City ___________________________________ State ____________ Country ___________________
Signature ________________________________________________ Date _____________________
Principal Operator Level of Experience
GC, Injector and Column Knowledge: Experienced Moderate Limited
Mass Spectrometer Theory Knowledge: Experienced Moderate Limited
TraceFi nde r Data System Knowledge: Experienced Moderate Limited
Additional Information
Have any special acceptance specifications been agreed to in the contract? Yes No 
If YES, attach full details of specifications.
Is there any additional equipment that needs to be interfaced to the system? Yes No
If YES, attach full details of additional equipment.
Note We reserve the right to invoice you for the Field Service Engineer’s time if the installation requirements are
not met on the date of the installation. To avoid any additional cost, please ensure your site is properly prepared.
1. All laboratory remodeling has been completed.
2. Your TSQ 8000 Evo system is on site.
3. The principal operator is scheduled to be available
during the installation / certification period.
4. Doorways, hallways, and so on are a minimum width of
81 cm (32 in.).
5. Available floor area is sufficient and flooring will
support the load.
6. Sufficient bench space is available for all of the
equipment. List the following:
Width: ________________________________
Depth: ________________________________
Height: ________________________________
7. Workbench can support the load of the system and is
free from vibration.
8. Lighting is adequate.
9. Work area is free of magnetic disruption and
electrostatic discharge.
10. Main power is installed and is in compliance with local
electrical codes.
11. Power for test and cleaning equipment is installed.
12. Match power cables specified on the sales order. Refer
to the power requirements for more information.
13. Voltage of power outlet has been measured. Note
measured voltage: ________________________
14. Air conditioning is adequate for temperature, humidity,
and particulate matter control. The laboratory can be
maintained at a constant temperature, between 15 and
35 °C (59 and 95 °F).
15. Relative humidity is between 5% and 95% with no
condensation.
16. All gases required are on site, gas lines are installed, and
gas regulators that terminate in 1/8” fittings are
available.
List gases and purity:______________________
17. All relevant safety regulations have been followed.
18. I have read and agree to the End User License
Agreements for any software purchased with my system:
Signature:

North America
United States
1400 North Point Pkwy #10
West Palm Beach, FL 33407
E-mail:
[e-mail]
Phone.........................[1] 800 532 4752
Fax .............................[1] 877 373 4006
Canada
2845 Argentia Road, Unit 4
Mississauga, Ontario, L5N 8G6
E-mail:
[e-mail]
Phone.........................[1] 800 530 8447
Fax .............................[1] [phone]
Europe
Austria
Wehlistrasse 27b
A-1200 Wien
E-mail: [e-mail]
Phone.........................[43] (0) 1 333 50 34-0
Fax .............................[43] (0) 1 333 50 34-26
Belgium
Clintonpark “Keppekouter”
Ninovesteenweg 198
B-9320 ERMEBODEGEM - AALST
E-mail: [e-mail]
Phone.........................[32] (0) 2 482 3030
Fax .............................[32] (0) 2 482 3031
Denmark
Fruebjergvej 3
2100 København Ø
E-mail: [e-mail]
Phone.........................[45] (70) 236267
Fax .............................[45] (70) 236263
Finland—see “Sweden, Norway, and Finland”
France
(Also representing French-speaking North Africa,
Algeria, Morocco, and Tunisia)
16 Avenue du Québec
Silic 765
Z.A. de Courtaboeuf
F-91963 Les Ulis Cédex
E-mail: [e-mail]
Phone.........................[33] (0) 1 60 92 49 50
Fax .............................[33] (0) 1 60 92 48 99
Germany
Im Steingrund 4-6
D-63303 Dreieich
E-mail: [e-mail]
Phone.........................[49] (0) 6103 408 1050
Fax .............................[49] (0) 6103 408 1213
Italy
Strada Rivoltana
I-20090 Rodano (Milano)
E-mail: [e-mail]
Phone.........................Numero Verde (800) 823 162
Fax .............................[39] (02) 95320 225
Netherlands
Takkebijsters 1
NL-4817 BL Breda
E-mail: [e-mail]
Phone.........................[31] (0) 76 579 55 55
Fax .............................[31] (0) 76 581 09 61
Norway—see “Sweden, Norway, and Finland”
Spain
C/Valportillo I, no22 1a Planta
Edificio Caoba
ES-28108 Alcobendas - Madrid
E-mail: [e-mail]
Phone.........................[34] (914) 845 965
Fax .............................[34](914) 843 598
Offices for Thermo Scientific CMD Products
For up-to-date contact information, visit www.thermoscientific.com.
Notes: The country code is enclosed in square brackets.
The city code or area code is enclosed in parenthesis ( ). For countries other than the U.S., when you are dialing from within the specified country, dial the 0 of the
city code. For countries other than Italy, when you are dialing from outside the country, do not dial the 0 of the city code.

Europe—continued
Sweden, Norway, and Finland
Pyramidbacken 3
S-141 75 Kungens Kurva (Stockholm)
Sweden
E-mail: [e-mail]
Phone.........................[46] (0) 8 556 468 20
Fax .............................[46] (0) 8 556 468 08
Switzerland
Neuhofstrasse 11
4153 Reinach
E-mail: [e-mail]
Phone.........................[41] (617) 16 77 40
Fax .............................[41] (617) 16 77 20
United Kingdom
Stafford House
1 Boundary Park
Boundary Way
Hemel Hempstead
Hertfordshire HP2 7GE
E-mail: [e-mail]
Phone ........................[44] (0) 870 241 1034
Fax .............................[44] (0) 1442 233 667
Australasia and Asia
Australia
P.O. Box 9092
5 Caribbean Drive
Scoresby, VIC 3179
E-mail: [e-mail]
Phone.........................[61] 39757 4300
Fax .............................[61] 9763 1169
Japan
C-2F
3-9 Moriya-cho, Kanagawa-ku
Yokohama 221-0022
E-mail: [e-mail]
Phone.........................[81] (45) 453 9100
Fax .............................[81] (45) 453 9110
P.R. China
7th Floor, 7F Tower West, Younghe Plaza
No. 28, Andingmen East Street
Dong Cheng District
Beijing 100007
E-mail: [e-mail]
Phone (free lines).......800 810 5118
...................................400 650 5118
Fax ............................[86] [phone]
Building 6
No.27 Xin Jinqiao Road
Pudong District
Shanghai 201206
Phone.........................[86] (21) 6865 4588
Fax ............................[86] (21) 6445 7830
Offices for Thermo Scientific CMD Products—Continued

Thermo Scientific TSQ 8000 Evo Preinstallation Requirements Guide vii
C
Preface . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .xiii
About Your System. . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .xiii
Related Documentation . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .xiv
System Requirements . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . xv
Safety and Special Notices . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . xv
Special Notices . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . xv
Safety Symbols and Signal Words . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .xvi
Hydrogen Safety Precautions . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . xvii
Using Hydrogen with TSQ 8000 Evo . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .xix
Hydrogen Connection Guidelines . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .xix
Purchasing Hydrogen . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .xxi
Properly Storing Hydrogen . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .xxi
Hydrogen Safety Codes, Standards and References . . . . . . . . . . . . . . . . . . .xxiii
Hazardous Substances Precautions . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . xxiv
Biological Hazard Warning Note. . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . xxiv
Venting Toxic Gases . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . xxv
Contacting Us . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . xxv
Chapter 1 Site Preparation. . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .1
Entrance Requirements . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 2
Workbench and Space Requirements . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 3
Lighting Requirements . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 6
Power Requirements . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 7
Environmental Requirements . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 11
Gas Equipment Requirements. . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 12
GC Carrier Gas . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 12
CI Gas. . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 13
Collision Gas. . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 14
Other Gas Specifications . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 15
Using Hydrogen . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 16
Receiving Requirements . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 16
What Happens Next? . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 17
Appendix A End User License Agreements . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .19
Contents

Contents
viii TSQ 8000 Evo Preinstallation Requirements Guide Thermo Scientific
End-User License Agreement–Apache OpenOffice 4.0.1 and TraceFinder
4.0. . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 19
End-User License Agreement–Microsoft Office Professional Plus 2013 and
TraceFinder 4.0. . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 27

Declaration
Manufacturer: Thermo Fisher Scientific
Thermo Fisher Scientific is the manufacturer of the instrument described in this manual and, as such, is responsible for
the instrument safety, reliability and performance only if:
• installation,
•recalibration,and
•changesandrepairs
have been carried out by authorized personnel and if:
• the local installation complies with local law regulations,
• the instrument is used according to the instructions provided, and
• if its operation is only entrusted to qualified trained personnel.
Thermo Fisher Scientific is not liable for any damages derived from the non-compliance with the aforementioned
recommendations.
Regulatory Compliance
Thermo Fisher Scientific performs complete testing and evaluation of its products to ensure full compliance with
applicable domestic and international regulations. When the system is delivered to you, it meets all pertinent
electromagnetic compatibility (EMC) and safety standards as described in the next section or sections by product name.
Changes that you make to your system may void compliance with one or more of these EMC and safety standards.
Changes to your system include replacing a part or adding components, options, or peripherals not specifically
authorized and qualified by Thermo Fisher Scientific. To ensure continued compliance with EMC and safety standards,
replacement parts and additional components, options, and peripherals must be ordered from Thermo Fisher Scientific
or one of its authorized representatives.
EMC and Safety Standards
• ITQ, and Ion Trap Series standards: EMC: EN 61326-1:2006. Safety: IEC 61010-1:2001, IEC 61010-2-081:2001
• Direct Probe Controller (DPC) standards: EMC: EN 61326-1:2013. Safety: IEC 61010-1:2001,
IEC 61010-2-081:2001
• ISQ Series standards: EMC: EN 61326-1:2013. Safety: IEC 61010-1:2010 (ed. 3); IEC 61010-2-081:2015 (ed.
2); IEC 61010-2-010:2014 (ed. 3); IECEE CB SCHEME CERT NO. DE 3-30000
• TSQ 8000 Evo and TSQ Duo standards: EMC: EN 61326-1:2013. Safety: IEC 61010-1:2010 (ed. 3); IEC
61010-2-081:2015 (ed. 2); IEC 61010-2-010:2014 (ed. 3); IECEE CB SCHEME CERT NO. DE 3-30034

Low Voltage Safety Compliance
This device complies with Low Voltage Directive 2011/95/EC.
FCC Compliance Statement
Notice on Lifting and Handling of
Thermo Scientific Instruments
For your safety, and in compliance with international regulations, the physical handling of this Thermo Fisher Scientific
instrument requires a team effort to lift and/or move the instrument. This instrument is too heavy and/or bulky for one
person alone to handle safely.
Notice on the Proper Use of
Thermo Scientific Instruments
In compliance with international regulations: Use of this instrument in a manner not specified by Thermo Fisher
Scientific could impair any protection provided by the instrument.
Notice on the Susceptibility
to Electromagnetic Transmissions
Your instrument is designed to work in a controlled electromagnetic environment. Do not use radio frequency
transmitters, such as mobile phones, in close proximity to the instrument.
THIS DEVICE COMPLIES WITH PART 15 OF THE FCC RULES. OPERATION IS SUBJECT TO
THE FOLLOWING TWO CONDITIONS: (1) THIS DEVICE MAY NOT CAUSE HARMFUL
INTERFERENCE, AND (2) THIS DEVICE MUST ACCEPT ANY INTERFERENCE RECEIVED,
INCLUDING INTERFERENCE THAT MAY CAUSE UNDESIRED OPERATION.
CAUTION Read and understand the various precautionary notes, signs, and symbols
contained inside this manual pertaining to the safe use and operation of this product before
using the device.

For manufacturing location, see the label on the instrument.
WEEE Compliance
This product is required to comply with the European Union’s Waste Electrical & Electronic Equipment (WEEE)
Directive 2002/96/EC. It is marked with the following symbol:
Thermo Fisher Scientific has contracted with one or more recycling or disposal companies in each European Union
(EU) Member State, and these companies should dispose of or recycle this product. See www.thermoscientific.com/
rohsweee for further information on Thermo Fisher Scientific’s compliance with these Directives and the recyclers in
your country.
WEEE Konformität
Dieses Produkt muss die EU Waste Electrical & Electronic Equipment (WEEE) Richtlinie 2002/96/EC erfüllen.
Das Produkt ist durch folgendes Symbol gekennzeichnet:
Thermo Fisher Scientific hat Vereinbarungen mit Verwertungs-/Entsorgungsfirmen in allen EU-Mitgliedsstaaten
getroffen, damit dieses Produkt durch diese Firmen wiederverwertet oder entsorgt werden kann. Mehr Information
über die Einhaltung dieser Anweisungen durch Thermo Fisher Scientific, über die Verwerter, und weitere Hinweise,
die nützlich sind, um die Produkte zu identifizieren, die unter diese RoHS Anweisung fallen, finden sie unter
www.thermoscientific.com/rohsweee.

Conformité DEEE
Ce produit doit être conforme à la directive européenne (2002/96/EC) des Déchets d'Equipements Electriques et
Electroniques (DEEE). Il est marqué par le symbole suivant:
Thermo Fisher Scientific s'est associé avec une ou plusieurs compagnies de recyclage dans chaque état membre de
l’union européenne et ce produit devrait être collecté ou recyclé par celles-ci. Davantage d'informations sur la
conformité de Thermo Fisher Scientific à ces directives, les recycleurs dans votre pays et les informations sur les
produits Thermo Fisher Scientific qui peuvent aider la détection des substances sujettes à la directive RoHS sont
disponibles sur www.thermoscientific.com/rohsweee.

Thermo Scientific TSQ 8000 Evo Preinstallation Requirements Guide xiii
P
Preface
This guide contains detailed information for preparing your site for installing a Thermo
Scientific™ TSQ 8000™ Evo triple-quadrupole mass spectrometer.
About Your System
Thermo Scientific systems provide the highest caliber gas chromatography/mass spectrometry
(GC/MS) instrumentation available on today’s market.
GC/MS represents a combination of two powerful analytical techniques: GC, which acts as a
separation technique, and MS, which acts as a detection technique. Complex mixtures of
individual compounds can be injected into the GC, either manually or by an autosampler and
then separated for presentation to the MS. The MS will generate a mass spectrum of the GC
eluate and its components. The mass spectrum can then be used for qualitative identification
as well as accurate and precise quantification of the individual compounds present in the
sample.
A triple-quadrupole GC/MS/MS system provides the extra selectivity required for trace
analysis of compounds in complex matrices.
Contents
•About Your System
•Related Documentation
•System Requirements
•Safety and Special Notices
•Hydrogen Safety Precautions
•Hazardous Substances Precautions
•Contacting Us

Preface
Related Documentation
xiv TSQ 8000 Evo Preinstallation Requirements Guide Thermo Scientific
Related Documentation
The TSQ 8000 Evo system includes Help and these manuals as PDF files:
•TSQ 8000 Evo Preinstallation Guide, PN 1R120586-0001
•TSQ 8000 Evo User Guide, PN 1R120586-0002
•TSQ 8000 Evo Hardware Manual, PN 1R120586-0003
•TSQ 8000 Evo Spare Parts Guide, PN 1R120586-0004
•TSQ 8000 AutoSRM User Guide, PN 1R120586-0005
•Q Exactive GC, ISQ LT, and TSQ 8000 Evo Direct Probe System User Guide, PN
1R120505-0006
To view product manuals
Go to the desktop and then Manuals > TSQ 8000 Evo.
To open Help
• From the TSQ 8000 Evo window, choose Help > TSQ 8000 Evo Help.
• If available for a specific window or dialog box, click Help or press the F1 key for
information about setting parameters.
For more information, visit www.thermoscientific.com.
WARNING Thermo Scientific systems operate safely and reliably under carefully
controlled environmental conditions. If the equipment is used in a manner not specified
by the manufacturer, the protections provided by the equipment might be impaired. If you
maintain a system outside the specifications listed in this guide, failures of many types,
including personal injury or death, might occur. The repair of instrument failures caused
by operation in a manner not specified by the manufacturer is specifically excluded from
the standard warranty and service contract coverage.

Preface
System Requirements
Thermo Scientific TSQ 8000 Evo Preinstallation Requirements Guide xv
System Requirements
Your TSQ 8000 Evo data system must meet these minimum requirements.
Safety and Special Notices
Make sure you follow the precautionary statements presented in this guide. The safety and
other special notices appear in boxes.
Special Notices
Special notices include the following:
System Requirements
Hardware • 4.6 GHz processor with 16GB RAM
•DVD/CD-ROMdrive
• Quad Core Processor
• Video card and monitor capable of 1680 × 1050 resolution
• 1000 GB hard drive
•NTFSformat
Software • Microsoft™ Windows™ 7 SP1 Operating System (64-bit)
• Thermo Scientific™ Xcalibur™ and Foundation1
• Thermo Scientific™ TraceFinder™2
1Check release notes for compatibility with TSQ Series instrument control software.
2Check release notes for compatibility with Thermo Foundation, Xcalibur, and TSQ 8000 Evo instrument
control software.
IMPORTANT Highlights information necessary to prevent damage to software, loss of
data, or invalid test results; or might contain information that is critical for optimal
performance of the system.
Note Highlights information of general interest.
Tip Highlights helpful information that can make a task easier.

Preface
Safety and Special Notices
xvi TSQ 8000 Evo Preinstallation Requirements Guide Thermo Scientific
Safety Symbols and Signal Words
All safety symbols are followed by WARNING or CAUTION, which indicates the degree of risk
for personal injury, instrument damage, or both. Cautions and warnings are following by a
descriptor. A WARNING is intended to prevent improper actions that could cause personal
injury. A CAUTION is intended to prevent improper actions that might cause personal injury
or instrument damage. You can find the following safety symbols on your instrument or in
this guide.
BIOHAZARD: Indicates that a biohazard will, could, or might occur.
BURN HAZARD: Alerts you to the presence of a hot surface that could or might
cause burn injuries.
ELECTRICAL SHOCK HAZARD: Indicates that an electrical shock could or
might occur.
FIRE HAZARD: Indicates a risk of fire or flammability could or might occur.
FLAMMABLE GAS HAZARD: Alerts you to gases that are compressed,
liquefied or dissolved under pressure and can ignite on contact with an
ignition source. This symbol indicates this risk could or might cause physical
injury.
GLOVES REQUIRED: Indicates that you must wear gloves when performing a
task or physical injury could or might occur.
HAND AND CHEMICAL HAZARD: Indicates that chemical damage or physical
injury could or might occur.
INSTRUMENT DAMAGE: Indicates that damage to the instrument or
component might occur. This damage might not be covered under the
standard warranty.
LIFTING HAZARD: Indicates that a physical injury could or might occur if two
or more people do not lift an object.

Preface
Hydrogen Safety Precautions
Thermo Scientific TSQ 8000 Evo Preinstallation Requirements Guide xvii
Hydrogen Safety Precautions
Hydrogen is a colorless, odorless, highly flammable gas with the molecular formula H2and an
atomic weight of 1.00794, making it the lightest element. Hydrogen gas presents a hazard as
it is combustible over a wide range of concentrations: at ambient temperature and pressure,
this ranges from about 4% to 74.2% by volume.
Hydrogen has a flash point of - 423 °F (- 253 °C) and an auto-ignition temperature of
1,040 °F (560 °C). It has a very low ignition energy and the highest burning velocity of any
gas. If hydrogen is allowed to expand rapidly from high pressure, it can self-ignite. Hydrogen
burns with a flame that can be invisible in bright light.
Before you begin using hydrogen, you should conduct a risk assessment based on the quantity
of hydrogen to be used and the conditions of your laboratory. You should ask yourself:
“What hydrogen hazards associated with this project are most likely to occur?”
MATERIAL AND EYE HAZARD: Indicates that eye damage could or might
occur.
RADIOACTIVE HAZARD: Indicates that exposure to radioactive material could
or might occur.
READ MANUAL: Alerts you to carefully read your instrument’s
documentation to ensure your safety and the instrument’s operational ability.
Failing to carefully read the documentation could or might put you at risk for a
physical injury.
TOXIC SUBSTANCES HAZARD: Indicates that exposure to a toxic substance
could occur and that exposure could or might cause personal injury or death.
For the prevention of personal injury, this general warning symbol precedes
the WARNING safety alert word and meets the ISO 3864-2 standard . In the
vocabulary of ANSI Z535 signs, this symbol indicates a possible personal
injury hazard exists if the instrument is improperly used or if unsafe actions
occur. This symbol and another appropriate safety symbol alerts you to an
imminent or potential hazard that could cause personal injury.
WARNING FIRE HAZARD: The use of hydrogen as a carrier gas is dangerous. Hydrogen is
potentially explosive and must be used with extreme care. Any use of hydrogen gas must
be reviewed by appropriate health and safety staff and all installations of hydrogen systems
must be performed to applicable codes and standards. Thermo Fisher Scientific assumes
no liability for the improper use of hydrogen as a carrier gas.

Preface
Hydrogen Safety Precautions
xviii TSQ 8000 Evo Preinstallation Requirements Guide Thermo Scientific
“What hydrogen hazards associated with this project have the potential to result in the
worst consequences?”
• Try to reduce or eliminate the higher risks by using the proper ventilation to remove
hydrogen gas before an ignitable concentration can accumulate. You should also consider
purging the hydrogen to further reduce hazards and ensure anyone who will be working
with hydrogen has basic hydrogen safety training.
• As with laboratory safety in general, be sure to wear safety glasses, laboratory coats, gloves,
etc. Typically there are no specific requirements for gaseous hydrogen, other than eye
protection when working with a compressed gas. If working with liquid (cryogenic)
hydrogen, insulated gloves and protective shoes should be worn in addition to eye
protection.
• You should post “No Smoking” and “No Open Flames” signs to identify hydrogen
sources and cylinders. Maintain, inspect and leak-test all hydrogen sources regularly.
• All hydrogen shutoff valves should be clearly marked and permanent hydrogen piping
should be labeled as such at the supply or discharge point and at regular intervals along its
length. Where hydrogen gas piping passes through a wall, the piping should be labeled on
both sides of the wall.
• There should also be contingency plans in place should an incident occur.
• The site emergency response team, as well as the local fire department, should know the
location of all hydrogen storage tanks.

Preface
Hydrogen Safety Precautions
Thermo Scientific TSQ 8000 Evo Preinstallation Requirements Guide xix
Using Hydrogen with TSQ 8000 Evo
To use hydrogen with the TSQ 8000 Evo, you must always shut off the GC carrier gas before
venting or turning off the TSQ 8000Evo. There are three hydrogen safety screws on the
TSQ 8000 Evo that must be in place. These are attached to your instrument at the factory.
Before powering on the TSQ 8000 Evo system, ensure that:
• All the covers and panels of the TSQ 8000 Evo system are firmly attached.
• The vent valve is tightly closed if you vented the system.
• All fittings, ferrules, and o-rings are sealed.
Hydrogen Connection Guidelines
Use the following guidelines to safely connect hydrogen to your system:
•Piping—Hydrogen must be delivered to equipment using appropriate piping and be
done in such a way as to pose essentially no hazard to end-users. Piping systems for the
delivery of hydrogen should be designed and installed by a person qualified by specific
training and experience with hydrogen piping systems.
Figure 1. Hydrogen Safety Screws on the TSQ 8000 Evo
Right Top Cover
Screw
Front Panel
Left Top
Cover

Preface
Hydrogen Safety Precautions
xx TSQ 8000 Evo Preinstallation Requirements Guide Thermo Scientific
Stainless steel is usually recommended because it is a safe, cost-effective material. Piping
of black iron or copper must not be used, as the pipe can become brittle with age.
Elastomeric/plastic tubing of various plastics and polymers should not be used, unless the
tubing is approved for use with hydrogen. If elastomeric/plastic tubing is used for
hydrogen gas delivery, the tubing should be tested for hydrogen permeability to minimize
leakage.
The hydrogen piping system must be flexible enough to endure routine thermal
expansion and contraction. The system should also include considerations for the most
severe condition of temperature and pressure expected during service. Piping and
supports must be able to withstand static loading introduced by such things as ice and
snow; and dynamic loading from high wind and earthquake.
Caution should be used if burying hydrogen piping. Proper controls should be used to
protect against damage and corrosion, and also to prevent Hydrogen from entering a
building if there is any leakage.
•Fittings—All fittings must be of the proper type approved or designed for use with
hydrogen gas. Use as few fittings as possible to minimize the potential for leaks. After
installation, ensure that leak testing is carried out prior to system use, and on a regular
basis.
There must be no PTFE tape or other things like plumber's putty used to enhance a seal, as
this actually is a detriment to a good seal. Ideally the best installation would use stainless
steel tubing with appropriate gas-tight fittings.
Welding is usually preferred for joints in hydrogen piping systems since welding provides
a better connection and reduces the potential for leaks compared to mechanical fittings.
Soft solder joints are not permitted for hydrogen systems (due to the low melting point of
soft solder and its potential for brittle failure at cryogenic temperatures). Brazed joints are
permitted, but such joints should be protected against the possibility of external fire.
Tubing connections should be clamped to barbed or press-fit type connections. Hose
clamps or jubilee clamps must not be used.
•Valves—All valves must be suitable for hydrogen service and for the specific operating
conditions. Valves, including regulators, must not be used for hydrogen, unless they are
designed and identified for such a use. Ball valves are often chosen because of their
superior leak tightness through the valve seat. Pneumatic operators are usually chosen for
remotely operated valves so that potential ignition sources (electricity) are remote from
the valve.
Manual shutoff valves should be provided near each point of use, within immediate reach.
If a hydrogen cylinder or hydrogen generation system is located within immediate reach,
a separate point-of-use shutoff valve is usually not necessary.
Line regulators that have their source away from the point of use should have a manual
shutoff valve near the point of use.