Thermo Scientific LH-4000W User manual
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Thermo Scientific 75006476 LH-4000W i
T
Preface ..................................................................................................................................... iii
Scope of Supply....................................................................................................................... iii
Precautions ..............................................................................................................................iii
Chapter 1 Rotor Data ................................................................................................................................ 1-1
Chapter 2 Accessories ............................................................................................................................ 2-1
Round Bucket 75006477 ........................................................................................ 2-2
Double Rectangular Bucket DoubleSpin 75006478 ................................................ 2-3
Chapter 3 Rotor Installation ...................................................................................................................3-1
Rotor Installation ..................................................................................................... 3-2
Removing the Rotor ................................................................................................ 3-2
Chapter 4 Rotor Loading .......................................................................................................................... 4-1
Before a Run ............................................................................................................ 4-2
Proper Loading ........................................................................................................ 4-2
Improper Loading .................................................................................................... 4-3
Maximum Loading .................................................................................................. 4-3
Cycle Counter ......................................................................................................... 4-4
Chapter 5 Aerosol-tight Applications ................................................................................................... 5-1
Basic Principles ........................................................................................................ 5-2
Closing aerosol-tight ................................................................................................ 5-2
Fill Level .................................................................................................................. 5-3
Checking the Aerosol-Tightness .............................................................................. 5-3
Chapter 6 Maintenance and Care ......................................................................................................... 6-1
Cleaning Intervals .................................................................................................... 6-2
Cleaning .................................................................................................................. 6-2
Disinfection ............................................................................................................. 6-3
Decontamination ..................................................................................................... 6-4
Autoclaving .............................................................................................................. 6-5
Service of Thermo Fisher Scientific .......................................................................... 6-5
RCF-Values ...............................................................................................................A-1
Chemical Compatibility Chart ...............................................................................B-1
Table of Contents
Technical Data......................................................................................................... 1-2
Thermo Scientific 75006476 LH-4000W iii
P
Preface
Before starting to use the rotor, read through this instruction manual carefully and follow the
instructions.
The information contained in this instruction manual is the property of Thermo Fisher Scientific; it is
forbidden to copy or pass on this information without explicit approval.
Failure to follow the instructions and safety information in this instruction manual will result in the
expiration of the sellers warranty.
Scope of Supply
If any parts are missing, please contact your nearest Thermo Fisher Scientific representative.
Precautions
Article Number Quantity Check
75006476 LH-4000W with Bucket 1
76003500 Grease for rubber seal 1
75003786 Boltgrease 1
50134706 CD with manual 1
This symbol refers to general hazards.
WARNING means that injuries or material damage or contamination could occur.
CAUTION means that material damage could occur.
This symbol refers to biological hazards.
Observe the information contained in the instruction manual to keep yourself and your
environment safe.
P Preface
iv 75006476 LH-4000W Thermo Scientific
In order to ensure safe operation of the LH-4000W rotor, the following general safety
regulations must be followed:
•Do not remove the magnet at the rotor bottom.
•Do not use rotors which showany signs of corrosion and/or cracks.
•Use only with rotors which have been loaded properly.
•Never overload the rotor.
•Operate the rotor always with the lid closed.
•Use only accessories which have been approved by Thermo Fisher Scientific. Exceptions
to this rule are commercially available glass or plastic centrifuge tubes, provided they
have been approved for the speed or the RCF value of the rotor.
•Please observe the safety instructions.
Please pay particular attention to the following aspects:
•Rotor installation: Check that the rotor is locked properly into place before operating
the centrifuge.
•Always balance the samples.
Maximum sample density at maximum speed: 1.2
g
cm3
-----------
1Rotor Data
1-2 75006476 LH-4000W Thermo Scientific
Technical Data
Technical Data
Table 1-1. 230 V,50 / 60 Hz Round Bucket 75006477
Centrifuge Cryofuge 5500i Cryofuge 5500i Multifuge 4KR
Catalog Number 75004471 75004479 75004461
Weight of empty Rotor [ kg ]18.4 18.4 18.4
Max. Cycle Number 30000 30000 30000
Maximum permissible Load [ g ] 4 x 1400 4 x 1400 4 x 1400
Maximum Speed nmax [ rpm ] 4400 4400 4400
Maximum RCF-Value at nmax 5346 5346 5346
Radius max. / min. [ cm ] 24.7 / 8.8 24.7 / 8.8 24.7 / 8.8
Acceleration / Braking Time [ s ] 95 / 85 95 / 85 95 / 85
Sample Heating at nmax [ °C ] referred to Ambient
Temperature of 23-25 °C, Run Time 60 Minutes
111
Max. Speed nmax [ rpm ] at 4 °C 4400 4400 4400
Aerosol-tight*
*tested by TÜV Product Service GmbH - Hamburg
yes (with cap 75006421) yes (with cap 75006421) yes (with cap 75006421)
Permissible temperature range autoclavable °C 121 121 121
Table 1-2. 230 V,50 / 60 Hz Double Rectangular Bucket DoubleSpin™2x 250 ml 75006478
Centrifuge Cryofuge 5500i Cryofuge 5500i Multifuge 4KR
Catalog Number 75004471 75004479 75004461
Weight of empty Rotor [ kg ]18.4 18.4 18.4
Max. Cycle Number 20000 20000 20000
Maximum permissible Load [ g ] 4 x 1500 4 x 1500 4 x 1500
Maximum Speed nmax [ rpm ] 4400 4400 4400
Maximum RCF-Value at nmax 4654 4654 4654
Radius max. / min. [ cm ] 21.5 / 11.5 21.5 / 11.5 21.5 / 11.5
Acceleration / Braking Time [ s ] 95 / 85 95 / 85 95 / 85
Sample Heating at nmax [ °C ] referred to Ambient
Temperature of 23-25 °C, Run Time 60 Minutes
111
Max. Speed nmax [ rpm ] at 4 °C 4400 4400 4400
Aerosol-tight*yes (with cap 75006479) yes (with cap 75006479) yes (with cap 75006479)
Permissible temperature range autoclavable °C 121 121 121
*tested by TÜV Product Service GmbH - Hamburg
1 Rotor Data
Thermo Scientific 75006476 LH-4000W 1-3
Table1-3. 230 V,50 / 60 Hz Double Rectangular Bucket 75006436
Centrifuge Cryofuge 5500i Cryofuge 5500i Multifuge 4KR
Catalog Number 75004471 75004479 75004461
Weight of empty Rotor [ kg ]17.9 17.9 17.9
Maximum Number of Cycles at Maximum Speed 12000 12000 12000
Maximum permissible Load [ g ] 4 x 1900 4 x 1900 4 x 1900
Maximum Speed nmax [ rpm ] 4400 4400 4400
Maximum RCF-Value at nmax 5476 5476 5476
Radius max. / min. [ cm ] 25.3 / 11.0 25.3 / 11.0 25.3 / 11.0
Acceleration / Braking Time [ s ] 110 / 90 110 / 90 110 / 90
Sample Heating at nmax [ °C ]referred to Ambient
Temperature of 23-25 °C, Run Time 60 Minutes
111
Max. Speed nmax [ rpm ] at 4 °C 4400 4400 4400
Aerosol-tight no no no
Autoclavable no no no
Table1-4. 200 V,50 Hz Round Bucket 75006477
Centrifuge Cryofuge 5500i
Catalog Number 75004479
Weight of empty Rotor [ kg ] 18.4
Max. Cycle Number 30000
Maximum permissible Load [ g ] 4 x 1400
Maximum Speed nmax [ rpm ] 4400
Maximum RCF-Value at nmax 5346
Radius max. / min. [ cm ] 24.7 / 8.8
Acceleration / Braking Time [ s ] 95 / 85
Sample Heating at nmax [ °C ]referred to Ambient
Temperature of 23-25 °C, Run Time 60 Minutes
4
Max. Speed nmax [ rpm ] at 4 °C 4400
Aerosol-tight*yes (with cap 75006421)
Permissible temperature range autoclavable °C 121
*tested by TÜV Product Service GmbH - Hamburg
Technical Data
1Rotor Data
1-4 75006476 LH-4000W Thermo Scientific
Table 1-5. 200 V Double Rectangular Bucket DoubleSpin™2 x 250 ml 75006478
Centrifuge Cryofuge 5500i
Catalog Number 75004479
Weight of empty Rotor [ kg ]18.4
Max. Cycle Number 20000
Maximum permissible Load [ g ] 4 x 1500
Maximum Speed nmax [ rpm ] 4400
Maximum RCF-Value at nmax 4654
Radius max. / min. [ cm ] 21.5 / 11.5
Acceleration / Braking Time [ s ] 95 / 85
Sample Heating at nmax [ °C ] referred to Ambient
Temperature of 23-25 °C, Run Time 60 Minutes
4
Max. Speed nmax [ rpm ] at 4 °C 4400
Aerosol-tight*yes (with cap 75006479)
Permissible temperature range autoclavable °C 121
*tested by TÜV Product Service GmbH - Hamburg
Table 1-6. 200 V,50 Hz Double Rectangular Bucket 75006436
Centrifuge Cryofuge 5500i
Catalog Number 75004479
Weight of empty Rotor [ kg ]17.9
Maximum Number of Cycles at Maximum Speed 12000
Maximum permissible Load [ g ] 4 x 1900
Maximum Speed nmax [ rpm ] 4400
Maximum RCF-Value at nmax 5476
Radius max. / min. [ cm ] 25.3 / 11.0
Acceleration / Braking Time [ s ] 110 / 90
Sample Heating at nmax [ °C ] referred to Ambient
Temperature of 23-25 °C, Run Time 60 Minutes
4
Max. Speed nmax [ rpm ] at 4 °C 4400
Aerosol-tight no
Autoclavable no
Technical Data
1 Rotor Data
Thermo Scientific 75006476 LH-4000W 1-5
Table1-7. 120 V,60 Hz Round Bucket 75006477
Centrifuge Cryofuge 5500i
Catalog Number 75004475
Weight of empty Rotor [ kg ]18.4
Max. Cycle Number 30000
Maximum permissible Load [ g ] 4 x 1400
Maximum Speed nmax [ rpm ] 4400
Maximum RCF-Value at nmax 5346
Radius max. / min. [ cm ] 24.7 / 8.8
Acceleration / Braking Time [ s ] 95 / 85
Sample Heating at nmax [ °C ]referred to Ambient
Temperature of 23-25 °C, Run Time 60 Minutes
1
Max. Speed nmax [ rpm ] at 4 °C 4400
Aerosol-tight*yes (with cap 75006421)
Permissible temperature range autoclavable °C 121
*tested by TÜV Product Service GmbH - Hamburg
Table1-8. 120 V,60 Hz Double Rectangular Bucket DoubleSpin™2 x 250 ml 75006478
Centrifuge Cryofuge 5500i
Catalog Number 75004475
Weight of empty Rotor [ kg ]18.4
Max. Cycle Number 20000
Maximum permissible Load [ g ] 4 x 1500
Maximum Speed nmax [ rpm ] 4400
Maximum RCF-Value at nmax 4654
Radius max. / min. [ cm ] 21.5 / 11.5
Acceleration / Braking Time [ s ] 95 / 85
Sample Heating at nmax [ °C ]referred to Ambient
Temperature of 23-25 °C, Run Time 60 Minutes
1
Max. Speed nmax [ rpm ] at 4 °C 4400
Aerosol-tight*yes (with cap 75006479)
Permissible temperature range autoclavable °C 121
*tested by TÜV Product Service GmbH - Hamburg
Technical Data
1Rotor Data
1-6 75006476 LH-4000W Thermo Scientific
Table 1-9. 120 V,60 Hz Double Rectangular Bucket 75006436
Centrifuge Cryofuge 5500i
Catalog Number 75004475
Weight of empty Rotor [ kg ]17.9
Maximum Number of Cycles at Maximum Speed 12000
Maximum permissible Load [ g ] 4 x 1900
Maximum Speed nmax [ rpm ] 4400
Maximum RCF-Value at nmax 5476
Radius max. / min. [ cm ] 25.3 / 11.0
Acceleration / Braking Time [ s ] 110 / 90
Sample Heating at nmax [ °C ] referred to Ambient
Temperature of 23-25 °C, Run Time 60 Minutes
1
Max. Speed nmax [ rpm ] at 4 °C 4400
Aerosol-tight no
Autoclavable no
Technical Data
1 Rotor Data
Technical Data
Thermo Scientific 75006476 LH-4000W 1-7
Table1-10. 230 V,50 / 60 Hz Round Bucket 75006477
Centrifuge
Catalog Number 75004473 75004481
Weight of empty Rotor [ kg ]18.4 18.4
Max. Cycle Number 30000 30000
Maximum permissible Load [ g ] 4 x 1400 4 x 1400
Maximum Speed nmax [ rpm ] 4400 4400
Maximum RCF-Value at nmax 5346 5346
Radius max. / min. [ cm ] 24.7 / 8.8 24.7 / 8.8
Acceleration / Braking Time [ s ] 95 / 85 95 / 85
Sample Heating at nmax [ °C ]referred to Ambient
Temperature of 23-25 °C, Run Time 60 Minutes
11
Max. Speed nmax [ rpm ] at 4 °C 4400 4400
Aerosol-tight*
*tested by TÜV Product Service GmbH - Hamburg
yes (with cap 75006421) yes (with cap 75006421)
Permissible temperature range autoclavable °C 121 121
Table1-11. 230 V,50 / 60 Hz Double Rectangular Bucket DoubleSpin™2 x 250 ml 75006478
Centrifuge
Catalog Number 75004473 75004481
Weight of empty Rotor [ kg ]18.4 18.4
Max. Cycle Number 20000 20000
Maximum permissible Load [ g ] 4 x 1500 4 x 1500
Maximum Speed nmax [ rpm ] 4400 4400
Maximum RCF-Value at nmax 4654 4654
Radius max. / min. [ cm ] 21.5 / 11.5 21.5 / 11.5
Acceleration / Braking Time [ s ] 95 / 85 95 / 85
Sample Heating at nmax [ °C ]referred to Ambient
Temperature of 23-25 °C, Run Time 60 Minutes
11
Max. Speed nmax [ rpm ] at 4 °C 4400 4400
Aerosol-tight*
*tested by TÜV Product Service GmbH - Hamburg
yes (with cap 75006479) yes (with cap 75006479)
Permissible temperature range autoclavable °C 121 121
Sorvall RC-4 Sorvall RC-4
Sorvall RC-4 Sorvall RC-4
1Rotor Data
1-8 75006476 LH-4000W Thermo Scientific
Table 1-12. 230 V,50 / 60 Hz Double Rectangular Bucket 75006436
Centrifuge
Catalog Number 75004473 75004481
Weight of empty Rotor [ kg ]17.9 17.9
Maximum Number of Cycles at Maximum Speed 12000 12000
Maximum permissible Load [ g ] 4 x 1900 4 x 1900
Maximum Speed nmax [ rpm ] 4400 4400
Maximum RCF-Value at nmax 5476 5476
Radius max. / min. [ cm ] 25.3 / 11.0 25.3 / 11.0
Acceleration / Braking Time [ s ] 110 / 90 110 / 90
Sample Heating at nmax [ °C ] referred to Ambient
Temperature of 23-25 °C, Run Time 60 Minutes
11
Max. Speed nmax [ rpm ] at 4 °C 4400 4400
Aerosol-tight no no
Autoclavable no no
Table 1-13. 200 V,50 Hz Round Bucket 75006477
Centrifuge
Catalog Number 75004481
Weight of empty Rotor [ kg ]18.4
Max. Cycle Number 30000
Maximum permissible Load [ g ] 4 x 1400
Maximum Speed nmax [ rpm ] 4400
Maximum RCF-Value at nmax 5346
Radius max. / min. [ cm ] 24.7 / 8.8
Acceleration / Braking Time [ s ] 95 / 85
Sample Heating at nmax [ °C ] referred to Ambient
Temperature of 23-25 °C, Run Time 60 Minutes
4
Max. Speed nmax [ rpm ] at 4 °C 4400
Aerosol-tight*yes (with cap 75006421)
Permissible temperature range autoclavable °C 121
*tested by TÜV Product Service GmbH - Hamburg
Sorvall RC-4 Sorvall RC-4
Sorvall RC-4
Technical Data
1 Rotor Data
Thermo Scientific 75006476 LH-4000W 1-9
Table1-14. 200 V,50 Hz Double Rectangular Bucket DoubleSpin™2 x 250 ml 75006478
Centrifuge
Catalog Number 75004481
Weight of empty Rotor [ kg ]18.4
Max. Cycle Number 20000
Maximum permissible Load [ g ] 4 x 1500
Maximum Speed nmax [ rpm ] 4400
Maximum RCF-Value at nmax 4654
Radius max. / min. [ cm ] 21.5 / 11.5
Acceleration / Braking Time [ s ] 95 / 85
Sample Heating at nmax [ °C ]referred to Ambient
Temperature of 23-25 °C, Run Time 60 Minutes
4
Max. Speed nmax [ rpm ] at 4 °C 4400
Aerosol-tight*yes (with cap 75006479)
Permissible temperature range autoclavable °C 121
*tested by TÜV Product Service GmbH - Hamburg
Table1-15. 200 V,50 Hz Double Rectangular Bucket 75006436
Centrifuge
Catalog Number 75004481
Weight of empty Rotor [ kg ]17.9
Maximum Number of Cycles at Maximum Speed 12000
Maximum permissible Load [ g ] 4 x 1900
Maximum Speed nmax [ rpm ] 4400
Maximum RCF-Value at nmax 5476
Radius max. / min. [ cm ] 25.3 / 11.0
Acceleration / Braking Time [ s ] 110 / 90
Sample Heating at nmax [ °C ]referred to Ambient
Temperature of 23-25 °C, Run Time 60 Minutes
4
Max. Speed nmax [ rpm ] at 4 °C 4400
Aerosol-tight no
Autoclavable no
Sorvall RC-4
Sorvall RC-4
Technical Data
1Rotor Data
1-10 75006476 LH-4000W Thermo Scientific
Table 1-16. 120 V,50 / 60 Hz Round Bucket 75006477
Centrifuge
Catalog Number 75004477
Weight of empty Rotor [ kg ]18.4
Max. Cycle Number 30000
Maximum permissible Load [ g ] 4 x 1400
Maximum Speed nmax [ rpm ] 4400
Maximum RCF-Value at nmax 5346
Radius max. / min. [ cm ] 24.7 / 8.8
Acceleration / Braking Time [ s ] 95 / 85
Sample Heating at nmax [ °C ] referred to Ambient
Temperature of 23-25 °C, Run Time 60 Minutes
1
Max. Speed nmax [ rpm ] at 4 °C 4400
Aerosol-tight*yes (with cap 75006421)
Permissible temperature range autoclavable °C 121
*tested by TÜV Product Service GmbH - Hamburg
Table 1-17. 120 V,50 Hz Double Rectangular Bucket DoubleSpin™ 2 x 250 ml 75006478
Centrifuge
Catalog Number 75004477
Weight of empty Rotor [ kg ]18.4
Max. Cycle Number 20000
Maximum permissible Load [ g ] 4 x 1500
Maximum Speed nmax [ rpm ] 4400
Maximum RCF-Value at nmax 4654
Radius max. / min. [ cm ] 21.5 / 11.5
Acceleration / Braking Time [ s ] 95 / 85
Sample Heating at nmax [ °C ] referred to Ambient
Temperature of 23-25 °C, Run Time 60 Minutes
1
Max. Speed nmax [ rpm ] at 4 °C 4400
Aerosol-tight*yes (with cap 75006479)
Permissible temperature range autoclavable °C 121
*tested by TÜV Product Service GmbH - Hamburg
Sorvall RC-4
Sorvall RC-4
Technical Data
1 Rotor Data
Thermo Scientific 75006476 LH-4000W 1-11
Table1-18. 120 V,50 / 60 Hz Double Rectangular Bucket 75006436
Centrifuge
Catalog Number 75004477
Weight of empty Rotor [ kg ]14.4
Maximum Number of Cycles at Maximum Speed 12000
Maximum permissible Load [ g ] 4 x 1400
Maximum Speed nmax [ rpm ] 4400
Maximum RCF-Value at nmax 5346
Radius max. / min. [ cm ] 24.7 / 8.8
Acceleration / Braking Time [ s ] 85 / 85
Sample Heating at nmax [ °C ]referred to Ambient
Temperature of 23-25 °C, Run Time 60 Minutes
10
Max. Speed nmax [ rpm ] at 4 °C4100
Aerosol-tight no
Autoclavable no
Sorvall RC-4
Technical Data
2-2 75006476 LH-4000W Thermo Scientific
2Accessories
Round Bucket 75006477
Round Bucket 75006477
CAUTION Close all sample containers before placing them in the bucket.
Table 2-1. Adapters and Accessories for Round Bucket 75006477
Centri-Lab®Adapter Type C
Max.
Dimensions x
Length*/ [ mm ]
Cap
[ mm ]
Tubes
per
rotor
Color Article Number
48 x 1.5 / 2 ml Mikroliter tubes 11.5 x 50 13.0 192 black 75008132
35 x 7 ml DIN 12.8 x 177 14.0 140 yellow 75008133
19 x 7 ml blood sampling 13.5 x 177 18.5 76 light grey 75008134
19 x 15 ml DIN 17.0 x 177 18.5 76 red 75008135
17 x 15 ml blood sampling 17.0 x 177 20 68 olive
brown
75008137
12 x 14 / 15 ml with flange 18.3 x 177 21.5 48 brown 75006494
7 x 25 ml DIN 25.0 x 177 31 28 orange 75008138
7 x 45 / 50 ml flat and round bottom 29.5 x 177 31 28 blue 75006493
4 x 50 ml DIN 34.5 x 177 39 16 green 75008140
5 x 50 ml conical 29.5 x 177 35.5 20 light
green
75006533
2 x 100 ml DIN 45.0 x 177 47.5 8 light blue 75008142
1 x 150 ml DIN / 180 ml bottle 56.6 x 132 / 128 4 white 75006498
1 x 175-225 ml conical** / 250 ml
bottle
62 x 177 75 4 nature 75008144
1 x 250 ml Bottle 59 x 190 4 nature 75006649
1 x 250 ml Corning®tube, conical 61.5 x 190 4 nature 75008147
1 x 500 ml Nalge®tube, conical 70 x 190 4 nature 75008145
1 x 500 ml Corning®tube, conical 96 x 190 4 nature 75006438
Bottle 1000 ml (Nominal volume) 100 x 190 75006613
Aerosol-tight caps with seals and lubricants (2 pieces) 75006421
Plastic insert “XL”for 400-500 ml blood bag system (2 pieces) 75006496
Plastic insert “M”for small volume special blood bank applications
(2 pieces)
75006485
Tare plates caoutchouc 2 x 35 and 65 g each 75005759
Tare weights for plastic insert
75006485
1 set of 4 weights, 6 g and 15 g each 75007645
*Max. Dimensions with aerosol-tight cap
** An additional pad obtainable from tube manufacturer is necessary.
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