Optika Italy B-380 Series User manual

Model
B-383POL
B-380 Series
INSTRUCTION MANUAL
Ver. 4.1 2020

Page 2
1. Warning 3
2. Symbols and conventions 3
3. Safety Information 3
4. Intended use 3
5. Overview 4
6. Unpacking 6
7. Assembling 6
7.1 Assembling the microscope 7
8. Summaryofbrighteldobservationprocedures 9
9. Useofthemicroscopeinbrighteld 10
9.1 Lightintensityadjustment 10
9.2 Adjusttheinterpupillarydistance 10
9.3 Diopteradjustment 10
9.4 Coarsefocustensionadjustment 10
9.5 Focuslocklever 11
9.6 Stage 11
9.7 Condensercentering 12
9.8 Aperturediaphragm 12
10. Use of the microscope in polarized light 13
10.1 Centering the nosepiece 13
10.2 Checking the extinction of light 14
10.3 Use of Tint plates 14
10.4 Use of Bertrand lens 15
11. Microphotography 16
11.1 Use of C-mount cameras 16
11.2 Useofreexcameras 16
12. Maintenance 17
13. Troubleshooting 18
Equipment disposal 20
Summary

Page 3
1. Warning
This microscope is a scientic precision instrument designed to last for many years with a minimum of
maintenance. It is built to high optical and mechanical standards and to withstand daily use. We remind you
that this manual contains important information on safety and maintenance, and that it must therefore be made
accessible to the instrument users. We decline any responsibility deriving from incorrect instrument use uses
that does not comply with this manual.
2. Symbols and conventions
The following chart is an illustrated glossary of the symbols that are used in this manual.
CAUTION
This symbol indicates a potential risk and alerts you to proceed with caution.
ELECTRICAL SHOCK
This symbol indicates a risk of electrical shock.
3. Safety Information
Avoiding Electrical Shock
Before plugging in the power supply, make sure that the supplying voltage of your region matches with the
operation voltage of the equipment and that the lamp switch is in off position. Users should observe all safety
regulations of the region. The equipment has acquired the CE safety label. However, users have full responsibility
to use this equipment safely. Please follow the guidelines below, and read this manual in its entirety to ensure
safe operation of the unit.
4. Intended use
Standard models
For research and teaching use only. Not intended for any animal or human therapeutic or diagnostic use.
IVDModels
Also for diagnostic use, aimed at obtaining information on the physiological or pathological situation of the
subject.

Page 4
5. Overview
PHOTO/TV PORT
OBSERVATION
HEAD
FOCUS LOCK
LEVER
MAIN SWITCH / LIGHT
INTENSITY DIAL
CONDENSER
STAGE
CONDENSER
HEIGHT
ADJUSTMENT
KNOB
NOSEPIECE
OBJECTIVES
CONDENSER
CENTERING SCREWS
FINE FOCUS
KNOB
COARSE FOCUS
KNOB
SLIDE HOLDER
BERTRAND
LENS

Page 5
EYEPIECES
DIOPTER
ADJUSTMENT
RING
ANALYZER
POLARIZER
TENSION
ADJUSTMENT
RING
APERTURE
DIAPHRAGM
Opposite side

Page 6
6. Unpacking
The microscope is housed in a moulded Styrofoam container. Remove the tape from the edge of the container
and lift the top half of the container. Take some care to avoid that the optical items (objectives and eyepieces)
fall out and get damaged. Using both hands (one around the arm and one around the base), lift the microscope
from the container and put it on a stable desk.
Do not touch with bare hands optical surfaces such as lenses, lters or glasses. Traces of grease or
other residuals may deteriorate the nal image quality and corrode the optics surface in a short time.
7. Assembling
Once opened the box, the microscope parts are the following:
① Frame
② Objectives
③ Observation head
④ Eyepieces
⑤ Bertrand lens
⑥ Analyzer
⑦ Tint plates
⑧ Tension adjustment tool
⑨ Allen wrenches
⑩ Dust cover
⑪ Power supply
①
③
④⑤
⑥⑦ ⑧
②
⑪
⑩⑨

Page 7
①
②
③
FFig. 1ig. 1
FFig. 2ig. 2
FFig. 3ig. 3
FFig. 4ig. 4
7.1 Assembling the microscope
1. Insert the Bertrand lens ① in the frame and lock
the locking screw ② with the provided Allen
wrench. (Fig.1)
2. Insert the optical head on the Bertrand lens and
lock the locking screw with the provided Allen
wrench. (Fig. 2)
3. Insert both eyepieces into the tubes of the optical
head. (Fig. 3)
• One of the two eyepieces is equipped with
a crosshair for centering the entire optical
system. It is advisable to insert the eyepiece
with crosshair in the right eyepiece holder.
• The condenser is pre-installed before leaving the
factory.
• To remove the condenser use an Allen wrench
diam. 1,5 and operate on the locking screw
placed on the right side of the condenser holder.
4. Remove the dummy slider from Bertrand lens
and insert the analyser ③.(Fig. 4)

Page 8
5. Insert the power supply jack in the connector
placed at the rear side of the microscope. (Fig. 5)
FFig. 5ig. 5

Page 9
Main switch / brightness
adjustment knob
(Used commands ) (Chapter)
Slide holder
Nosepiece
Observation head
Diopter adjustment ring
Condenser centering
Aperture diaphragm
Nosepiece
Main switch /
brightness adjustment knob
Coarse and fine focus knobs
Coarse and fine focus knobs
Set the main swith to “ON” and adjust light intensity
Place a slide on the stage
Insert 10x objective into the light path
Focus the specimen
Begin observation
Adjust aperture diaphragm
Insert in the light path the desired objective and focus the
specimen
Adjust interpupillary distance
Adjust diopter
Center optical axis
Adjust brightness
9
9
5
5
5
5
9
9
9
9
9
Remove all the filters from the light path
Bertrand lens
Set on “O” the Bertrand lens ring 10
Analyzer 10
8. Summaryofbrighteldobservationprocedures
Tint plates 10

Page 10
①
②
③
9. Useofthemicroscopeinbrighteld
9.1 Lightintensityadjustment
1. Operate on the light intensity dial ① to turn ON/
OFF the microscope and to increase or decrease
the illumination intensity. (Fig. 6)
9.2 Adjusttheinterpupillarydistance
1. Hold the right and left parts of the observation
head using both hands and adjust the interpupil-
lary distance by turning the two parts until one
circle of light can be seen. (Fig. 7)
• The graduation on the interpupillary distance in-
dicator, pointed by the spot “.” on the eyepiece
holder, shows the distance between the opera-
tor’s eyes.
• The range of the interpupillary distance is 48- 75
mm
9.3 Diopteradjustment
1. Look into the right eyepiece with your right eye
only, and focus on the specimen.
2. Look into the left eyepiece with your left eye only.
If the image is not sharp, use the dioptric adjust-
ment ring ② to compensate. (Fig. 8)
• Highpoint eyepieces allow the use also to glass
wearers.
• NOTE: For optimal parfocality, we recommend
using your glasses during normal microscope use
9.4 Coarsefocustensionadjustment
• Adjustthetensionusingtheprovidedtool.
The coarse knob tension is pre-setted in the factory.
1. To modify the tension according to personal’s
needs, rotate the ring ③ using the provided tool
(Fig. 9).
• Clockwise rotation increases the tension.
• If the tension is too loose, the stage could go
lower by itself or the focus easily lost after ne
adjustment. In this case, rotate the knob in order
to increase the tension.
FFig. 6ig. 6
FFig. 7ig. 7
FFig. 8ig. 8
FFig. 9ig. 9

Page 11
9.5 Focuslocklever
The upper limit knob has two functions: prevent the
contact between slide and objective and acts as
“focus memory”.
1. After focussing the specimen, pull the lever ①
toward the front of the microscope and lock it.
(Fig. 9).
• In this way the focus upper limit is set.
2. Now one can lower the stage with coarse focus
knob, replace the specimen and raise again the
stage up to the upper limit: specimen will be in
approximate focus and will need a ne adjustment
to get the proper focus..
• Fine focus movement is not affected by the
coarse focus lock.
• To unlock, move the lever in the opposite
direction to the one used for the locking.
9.6 Stage
• The rotating stage accepts specimens on slide.
• It is possible to lock the specimen once placed
on the stage using the stage clips ② . (Fig. 11)
1. After loosing the locking screw ③ , the stage can
be rotated by 360°
FFig. 11ig. 11
②
③
FFig. 10ig. 10
①
LOCK
UNLOCK

Page 12
9.7 Condensercentering
• The condenser is installed and pre-centred in the
factory.
Should a new centering is needed, operate in this
way:
1. Insert 4x objective in the light path (in case 4x is
not available use the lower magnication avail-
able).
2. Focus the specimen.
3. Close the aperture diaphragm using the ring ①,
moving the ring to the value “4” related to the 4x
objective. (Fig. 26)
4. Raise the condenser to the upper limit using the
height adjustment knob ② placed on the left side
of the condenser holder.
5. Center the condenser using the centering screws
③ until the eld of view is evenly illuminated (in
the eld of view no dark and bright areas must be
noticed).
6. Fully open the diaphragm.
9.8 Aperturediaphragm
• The Numerical Aperture (N.A.) value of the ap-
erture diaphragm affects the image contrast. In-
creasing or reducing this value one can vary res-
olution, contrast and depth of focus of the image.
Move the diaphragm ring ① (Fig. 13) on the value
corresponding to the objective in use. In this case
the optimal setting of the condenser is achieved.
• With low contrast specimens set the numerical
aperture to about 70%-80% of the objective’s
N.A. If necessary, remove on eyepiece and, look-
ing into empty sleeve, adjust the condenser’s dia-
phragm in order to obtain an image like the one
in Fig. 14.
FFig. 12ig. 12
①
②
③
FFig. 13ig. 13
APERTURE
DIAPHRAGM
FIELD OF VIEW
30-20%
70-80%

Page 13
10. Use of the microscope in polarized light
10.1 Centering the nosepiece
1. Loosen the stage rotation lock ① and rotate the
stage until the graduated scale of the stage ②
and the vernier scale ③ are aligned in the “0”
position. (Fig. 14)
• This operation serves to ensure a standard
reference position for centering the nosepiece.
2. Focus on a recognizable detail ④ in the eld of
view by placing it at the centre of the crosshair.
(Fig. 15)
3. By turning the stage, the detail in focus will
describe a circle ⑤. (Fig. 15)
4. Move again the stage on the “0” position and lock
the locking screw ①.
5. Operating on the nosepiece centering screws ⑥
move the detail in diametrically opposite direction
to the described circle. The movement must be
about half the diameter of the circle described.
(Fig. 16)
6. Manually move the specimen and put it again
on the centre of the crossahir. Loosen again the
stage rotation lock and rotate once more the
stage.
7. If the centering has been carried out correctly, by
turning the table the image of the focused detail
does not move with respect to the centre of the
crosshair. If not, repeat the operations described
by 2. to 6. until the perfect coincidence of the
centre of rotation of the stage with the centre of
the crosshair for which the specimen remains in
the centre of the crosshair turning the table.
8. Once centred with the 10x, rotate the nosepiece
to insert in the optical path all the other objectives
and verify the correct centering of the objectives,
acting on the screws of centering the nosepiece
⑥, to make sure that all the objectives are
perfectly centred with respect to the optical axis.
①
②
③
④⑤
• The system allows observation in Orthoscopy (crossed Nicol) or in Conoscopy (crossed Nicol with
the use of the Bertrand lens).
• Foroptimalperformanceinpolarizedlightmicroscopy,accurateopticaladjustmentsareessential
before beginning the observation.
FFig. 14ig. 14
FFig. 15ig. 15
FFig. 16ig. 16
⑥
⑥

Page 14
10.2 Checking the extinction of light
1. Remove the specimen from the light path and
insert 10x objective.
2. Loosen the locking screw of the polarizer ① and
check that the scale is in the “0” position ②. (Fig.
17)
3. Insert rotatable analyser in the light path, loosen
the locking screw ③ and put the vibration scale
on 0° ④, then lock the locking screw ③. (Fig.
18)
4. Loosen the polarizer locking screw ① and rotate
the polarizer scale ② to obtain total extinction
(total dark in the eyepieces or “Crossed Nicol
position”). Tighten the screws ①. (Fig. 17)
• It may happen that the scale of the polarizer
is not perfectly aligned on the reference mark
but is shifted by one or two notches. This
is not a defect but is due to the mechanical
alignment of the polarizers during assembly.
10.3 Use of Tint plates
Three tint plates are supplied with the microscope:
• l plate (1st order Red)
• l/4 plate
• “Quartz wedge” plate (Q)
1. Put a specimen on the stage and focus.
2. Insert in the right slot of the Bertrand lens ⑤ one
of the tint plates ⑥. (Fig. 19)
3. In polarized light, inserting one of the plates will
have chromatic effects on the specimen.
• Using the l plate (also called 1st order Red) the
specimen will take a magenta tinge.
• Using the l/4 plate the specimen will take on a
colour tending to pale yellow.
• Using the Q plate the specimen will present a
series of coloured bands that will fade as the
plate is inserted.
①
②FFig. 17ig. 17
FFig. 18ig. 18
FFig. 19ig. 19
③
④
⑤
⑥

Page 15
10.4 Use of Bertrand lens
• Bertrand lens allows observation in Orthoscopy
and Conoscopy.
• In the disengaged position (“O”) the lens allows
observation in Orthoscopy, while in the inserted
position (“B”) it is possible to make observations
in Conoscopy.
1. Rotate the upper knurled ring of the Bertrand lens
① to engage the “B” position. (Fig. 20)
2. Using one objective from 20x to 60x, focus the
conoscopic image using the focus ring ②.
3. If the conoscopic image is not perfectly centred
with respect to the optical axis, centre the image
using the centering screws ③.
• By turning the stage you will see black fringes
that will appear and disappear depending on
the rotation of the stage. These fringes are the
crystallization axes of that specic crystal. (Fig.
21)
FFig. 20ig. 20
FFig. 21ig. 21
①
②
③

Page 16
11. Microphotography
①
②
③
④
11.1 Use of C-mount cameras
1. Loosen the clamping screw ① on the trinocular
port and remove the dust cap②. (Fig. 22)
2. Screw the C-mount adapter ③to the camera ④
and insert the round dovetail of the C-mount into
the empty hole of the trinocular port, then tighten
the clamping screw ①. (Fig. 23)
11.2 Useofreexcameras
1. Insert the Reex adapter ① into the relay tube to
the microscope ②.
2. Screw the “T2” ring ③ (not provided) to the reex
adapter.
3. Connect the Reex camera ④ to the “T2” just
installed (Fig. 24).
4. Mount the other end of the relay tube ② into the
empty hole of the trinocular port, then tighten the
clamping screw. (Fig. 22)
• “T2” ring is not provided with the microscope, but
is commercially available.
• While shooting dark specimens, darken eyepieces
and viewnder with a dark cloth to minimize the
diffused light.
• To calculate the magnication of the camera:
objective magnication * camera magnication *
lens magnication.
• If using an SLR camera, mirror movement
may cause the camera to vibrate.
• We suggest lifting the mirror, using long
exposure times and a remote cord.
FFig. 22ig. 22
FFig. 23ig. 23
FFig. 24ig. 24
①
②
③
④

Page 17
12. Maintenance
Microscopy environment
This microscope is recommended to be used in a clean, dry and shock free environment with a temperature of
5°-40°C and a maximum relative humidity of 85 % (non condensing). Use a dehumidier if needed.
To think about when and after using the microscope
• The microscope should always be kept vertically when moving it and be careful so that no
moving parts, such as the eyepieces, fall out.
• Never mishandle or impose unnecessary force on the microscope.
• Never attempt to service the microscope yourself.
• After use, turn off the light immediately, cover the microscope with the provided dust-cover,
and keep it in a dry and clean place.
Electrical safety precautions
• Before plugging in the power supply, make sure that the supplying voltage of your region
matches with the operation voltage of the equipment and that the lamp switch is in off-
position.
• Users should observe all safety regulations of the region. The equipment has acquired the
CE safety label. However, users do have full responsibility to use this equipment safely.
Cleaning the optics
• If the optical parts need to be cleaned try rst to: use compressed air.
• If that is not sufcient: use a soft lint-free piece of cloth with water and a mild detergent.
• And as a nal option: use the piece of cloth moistened with a 3:7 mixture of ethanol and ether.
• Note: ethanol and ether are highly ammable liquids. Do not use them near a heat source, near
sparks or near electric equipment. Use these chemicals in a well ventilated room.
• Remember to never wipe the surface of any optical items with your hands. Fingerprints can damage the
optics.
• Do not disassemble objectives or eyepieces in attempt to clean them.
Forthebestresults,usetheOPTIKAcleaningkit(seecatalogue).
If you need to send the microscope to Optika for maintenance, please use the original packaging.

Page 18
13. Troubleshooting
Review the information in the table below to troubleshoot operating problems.
PROBLEM CAUSE SOLUTION
I. Optical Section:
LED operates, but eld of view remains
dark. Power supply is unplugged. Connect
Brightness is too low Set brightness to a proper level
Bertrand lens is in Remove Bertrand lens from light path
You are in a position of extinction Disengage analyzer from the light path
Field of view is obscured or not evenly
illuminated Nosepiece is not correctly engaged. Make sure that the nosepiece clicks properly into place.
Tint plate or Bertrand lens are in a in-
termediate position. Move to a click stop
Dirt or dust is visible in the field of view. Dirt/dust on the specimen Clean the specimen
Dirt/dust on the eyepieces Clean the eyepieces
Image looks double Aperture iris diaphragm is stopped
down too far. Open aperture iris diaphragm.
The condenser is not well centered or
it is in a wrong height Set the condenser until a proper image is achieved
Visibility is poor.
• Image is not sharp.
• Contrast is poor
• Details are indistinct
• Image glares
Nosepiece is in an incorrect position Move the nosepiece to a click stop
Aperture iris diaphragm is too closed
or too open. Adjust aperture iris diaphragm.
Dust or dirt on lenses (condenser,
objectives, eyepieces and slide) Clean thoroughly.
For transmitted light observation, the
coverglass thickness must not exceed
0.17mm
Use a coverglass with thickness
0.17mm
Focus is not even Slide holder is not at. Move the speci-
men to a at position.
One side of the image is unfocused Nosepiece is in an incorrect position Move the nosepiece to a click stop
Slide is mounted not in a at position
(tilted) Place the specimen in a at position on the stage
Poor quality of the glass slide Use a glass slide with higher quality
Conoscopic image cannot be seen Bertrand lens is not in the light path. Insert the lens in the light path
Total extinction cannot be obtained Analyzer is not in the light path Insert analyzer in the light path.
II. Mechanical Section:
Coarse focus knob is hard to turn Tension adjustment ring is too tight Loosen tension adjustment ring
Focus is unstable Tension adjustment ring is too loose Tighten tension adjustment ring
III. Electrical Section
LED doesn’t turn on. Power supply not connected Check for proper connection
Brightness is not enough Brightness setting is too low Adjust brightness
Light blinks Power supply not well connected Check for proper connection
IV. Observation tube
Field of view of one eye does not match
that of the other. Interpupillary distance is incorrect. Adjust interpupillary distance.
Incorrect diopter adjustment. Adjust diopter.
Your view is not accustomed to micro-
scope observation. Upon looking into eyepieces, try look- ing at overall field before concentrating
on specimen range. You may also find
it helpful to look up and into distance
for a moment before looking back into
microscope.

Page 19
V. Microphotography
IImage edge is unfocused To a certain extent it is due to achro-
matic objectives features To minimize the problem, set the aper- ture diaphragm in a proper position
Bright spots appear on the image Stray light entering in the microscope
through eyepieces or camera viewn-
der
Cover eyepieces and viewnder with
a dark cloth

Page 20
Equipment disposal
Art.13 Dlsg 25 July 2005 N°151. “According to directives 2002/95/EC, 2002/96/EC and 2003/108/EC relating
to the reduction in the use of hazardous substances in electrical and electronic equipment and waste disposal.”
The basket symbol on equipment or on its box indicates that the product at the end of its useful life should be
collected separately from other waste. The separate collection of this equipment at the end of its lifetime is or-
ganized and managed by the producer. The user will have to contact the manufacturer and follow the rules that
he adopted for end-of-life equipment collection. The collection of the equipment for recycling, treatment and
environmentally compatible disposal, helps to prevent possible adverse effects on the environment and health
and promotes reuse and/or recycling of materials of the equipment. Improper disposal of the product involves
the application of administrative penalties as provided by the laws in force.