Thermo Scientific Heraeus Cryofuge 5500i User manual

Thermo Scientific
Diagnostik Rotor
Instruction Manual
50135351-b• 07/ 2020
Visit us online to register your warranty:
thermofisher.com/labwarranty

WEEE Conformity
This product is subject to the regulations of the EU Waste Electrical & Electronic Equipment
(WEEE) Directive 2012/19/EU. It is marked by the following symbol:

Thermo Scientific 75006480 DiagnostikTM Rotor i
T
Preface ...................................................................................................................................... iii
Scope of Supply ....................................................................................................................... iii
Precautions .............................................................................................................................. iv
Chapter 1 Rotor Data ................................................................................................................................ 1-1
Technical Data ........................................................................................................ 1-2
Chapter 2 Accessories ............................................................................................................................ 2-1
Chapter 3 Rotor Installation ...................................................................................................................3-1
Rotor Installation ..................................................................................................... 3-2
Removing the Rotor ................................................................................................ 3-2
Chapter 4 Rotor Loading .......................................................................................................................... 4-1
Before a Run ............................................................................................................ 4-2
Proper Loading ........................................................................................................ 4-2
Improper Loading .................................................................................................... 4-3
Chapter 5 Maintenance and Care ......................................................................................................... 5-1
Cleaning Intervals .................................................................................................... 5-2
Cleaning .................................................................................................................. 5-2
Disinfection ............................................................................................................. 5-3
Decontamination ..................................................................................................... 5-4
Service of Thermo Fisher Scientific .......................................................................... 5-5
RCF-Values ...............................................................................................................A-1
Chemical Compatibility Chart ...............................................................................B-1
Table of Contents

C Contents
ii 75006480 DiagnostikTM Rotor Thermo Scientific

Thermo Scientific 75006480 DiagnostikTM Rotor iii
P
Preface
Before starting to use the rotor, read through this instruction manual carefully and follow the
instructions.
The information contained in this instruction manual is the property of Thermo Fisher Scientific; it is
forbidden to copy or pass on this information without explicit approval.
Failure to follow the instructions and safety information in this instruction manual will result in the
expiration of the sellers warranty.
Scope of Supply
If any parts are missing, please contact your nearest Thermo Fisher Scientific representative.
Article Number Quantity Check
75006480 Diagnostik Rotor 1
75003786 BoltGrease 1
50134706 CD with Manual 1
This symbol refers to general hazards.
WARNING means that injuries or material damage or contamination could occur.
CAUTION means that material damage could occur.
This symbol refers to biological hazards.
Observe the information contained in the instruction manual to keep yourself and your
environment safe.

P Preface
iv 75006480 DiagnostikTM Rotor Thermo Scientific
Precautions
In order to ensure safe operation of the diagnostic rack rotor, the following general safety regulations
must be followed:
•Do not remove the magnet at the rotor bottom.
•Do not use rotors which show any signs of corrosion and/or cracks.
•Use only with rotors which have been loaded properly.
•Never overload the rotor.
•Operate the rotor always with the lid closed.
•Use only accessories which have been approved by Thermo Fisher Scientific. Exceptions to this
rule are commercially available glass or plastic centrifuge tubes, provided they have been approved
for the speed or the RCF value of the rotor.
•Please observe the safety instructions.
Please pay particular attention to the following aspects:
•Rotor installation: Check that the rotor is locked properly into place before operating the
centrifuge.
•Always balance the samples.
Maximum sample density at maximum speed: 1.2
g
cm3
-----------

Thermo Scientific 75006480 DiagnostikTM Rotor 1-1
1
Rotor Data
Contents
•“Technical Data” on page 1-2

1 Rotor Data
Technical Data
1-2 75006480 DiagnostikTM Rotor Thermo Scientific
Technical Data
Table 1-1. 230 V, 50 / 60 Hz
Centrifuge Cryofuge 5500i Cryofuge 5500i Multifuge 4KR
Catalog Number 75004471 75004479 75004461
Weight of empty Rotor [kg] 10.7 10.7 10.7
Maximum permissible Load [ g ] 2 x 2000 2 x 2000 2 x 2000
Maximum Speed nmax [ rpm ] 3500 3500 3500
Maximum RCF-Value at nmax 2547 2547 2547
Radius max. / min. [ cm ] 18.6 / 14.1 18.6 / 14.1 18.6 / 14.1
Acceleration / Braking Time [ s ] 65 / 65 65 / 65 60 / 65
Sample Heating at nmax [° C] referred to Ambient
Temperature of 23-25 °C, Run Time 60 Minutes
< 0 < 0 < 0
Max Speed nmax [ rpm ] at 4 °C 3500 3500 3500
Aerosol-tight No No No
Autoclavable No No No
Table 1-2. 200 V, 50 Hz
Centrifuge Cryofuge 5500i
Catalog Number 75004479
Weight of empty Rotor [kg] 10.7
Maximum permissible Load [ g ] 2 x 2000
Maximum Speed nmax [ rpm ] 3500
Maximum RCF-Value at nmax 2547
Radius max. / min. [ cm ] 18.6 / 14.1
Acceleration / Braking Time [ s ] 65 / 65
Sample Heating at nmax [° C] referred to Ambient
Temperature of 23-25 °C, Run Time 60 Minutes
< 0
Max Speed nmax [ rpm ] at 4 °C 3500
Aerosol-tight No
Autoclavable No

1 Rotor Data
Technical Data
Thermo Scientific 75006480 DiagnostikTM Rotor 1-3
Table 1-3. 120 V, 60 Hz
Centrifuge Cryofuge 5500i
Catalog Number 75004475
Weight of empty Rotor [kg] 10.7
Maximum permissible Load [ g ] 2 x 2000
Maximum Speed nmax [ rpm ] 3500
Maximum RCF-Value at nmax 2547
Radius max. / min. [ cm ] 18.6 / 14.1
Acceleration / Braking Time [ s ] 65 / 65
Sample Heating at nmax [° C] referred to Ambient
Temperature of 23-25 °C, Run Time 60 Minutes
< 0
Max Speed nmax [ rpm ] at 4 °C 3500
Aerosol-tight No
Autoclavable No
Table 1-4. 230 V, 50 / 60 Hz
Centrifuge Sorvall RC-4 Sorvall RC-4
Catalog Number 75004473 75004481
Weight of empty Rotor [kg] 10.7 10.7
Maximum permissible Load [ g ] 2 x 2000 2 x 2000
Maximum Speed nmax [ rpm ] 3500 3500
Maximum RCF-Value at nmax 2547 2547
Radius max. / min. [ cm ] 18.6 / 14.1 18.6 / 14.1
Acceleration / Braking Time [ s ] 65 / 65 65 / 65
Sample Heating at nmax [° C] referred to Ambient
Temperature of 23-25 °C, Run Time 60 Minutes
< 0 < 0
Max Speed nmax [ rpm ] at 4 °C 3500 3500
Aerosol-tight No No
Autoclavable No No

1 Rotor Data
Technical Data
1-4 75006480 DiagnostikTM Rotor Thermo Scientific
Table 1-5. 200 V, 50 Hz
Centrifuge Sorvall RC-4
Catalog Number 75004481
Weight of empty Rotor [kg] 10.7
Maximum permissible Load [ g ] 2 x 2000
Maximum Speed nmax [ rpm ] 3500
Maximum RCF-Value at nmax 2547
Radius max. / min. [ cm ] 18.6 / 14.1
Acceleration / Braking Time [ s ] 65 / 65
Sample Heating at nmax [° C] referred to Ambient
Temperature of 23-25 °C, Run Time 60 Minutes
< 0
Max Speed nmax [ rpm ] at 4 °C 3500
Aerosol-tight No
Autoclavable No
Table 1-6. 120 V, 60 Hz
Centrifuge RC-4
Catalog Number 75004477
Weight of empty Rotor [kg] 10.7
Maximum permissible Load [ g ] 2 x 2000
Maximum Speed nmax [ rpm ] 3500
Maximum RCF-Value at nmax 2547
Radius max. / min. [ cm ] 18.6 / 14.1
Acceleration / Braking Time [ s ] 65 / 65
Sample Heating at nmax [° C] referred to Ambient
Temperature of 23-25 °C, Run Time 60 Minutes
< 0
Max Speed nmax [ rpm ] at 4 °C 3500
Aerosol-tight No
Autoclavable No

Thermo Scientific 75006480 DiagnostikTM Rotor 2-1
2
Accessories
Contents
•“Accessory“ on page 2-2

2 Accessories
2-2 75006480 DiagnostikTM Rotor Thermo Scientific
Table 2. Adapters and Accessories for Rotor 75006480
Adapter for Diagnostik
Rack Rotor
Length / Width
[mm]
Maximum Height incl.
Tubes and Rack [mm]
Racks per
Rotor Article Number
Hitachi 118 x 20 120 2 x 10 75006416
Olympus 177 x 20 120 2 x 6 75006417
Sysmex 200 x 25 120 2 x 6 75006418
Dade-Behring -- 120 2 x 2 75006419

Thermo Scientific 75006480 DiagnostikTM Rotor 3-1
3
Rotor Installation
Contents
•“Rotor Installation” on page 3-2
•“Removing the Rotor” on page 3-2

3 Rotor Installation
Rotor Installation
3-2 75006480 DiagnostikTM Rotor Thermo Scientific
Rotor Installation
Use only the rotors with approved accessories.
Proceed as follows:
1. Open the door of the centrifuge and if necessary remove any dust, foreign objects or residue from
the chamber.
Thread and o-ring must be clean and undamaged.
2. Hold the rotor over the centrifuge spindle.
3. Let the rotor slide slowly down the centrifuge spindle.
4. Thread the fastening tool into the centrifuge spindle clockwise. Hold the rotor with the other hand
into position.
5. Check if the rotor is properly installed by lifting it slightly on the handle.
6. If available, close the rotor with the rotor lid.
Removing the Rotor
To remove the rotor, proceed as follows:
1. Open the centrifuge door.
2. Thread the fastening tool in the centrifuge spindle counter-clockwise. Hold the rotor with the
other hand into position.
3. At the same time, pull the rotor directly upwards with both hands and remove it from the
centrifuge spindle. Make sure not to tilt the rotor while doing this operation.
CAUTION Unapproved or incorrectly combined accessories can cause serious damage to
the centrifuge.
CAUTION Do not force the rotor onto the centrifuge spindle.
WARNING Check for any damage to the rotor: Damaged rotors must not be used.
Keep the centrifuge spindle area of the rotor clear of objects.
CAUTION Check that the rotor is properly locked on the centrifuge spindle before each use
by pulling it at its handle.

Thermo Scientific 75006480 DiagnostikTM Rotor 4-1
4
Rotor Loading
Contents
•“Before a Run” on page 4-2
•“Proper Loading” on page 4-2
•“Improper Loading” on page 4-3

4 Rotor Loading
Before a Run
4-2 75006480 DiagnostikTM Rotor Thermo Scientific
Before a Run
1. Please read and observe the safety instructions contained in this operating instruction and the
instructions for use.
2. Check the rotor and all accessory parts for damages such as cracks, scratches or traces of corrosion.
3. Check the rotor chamber, centrifuge spindle and the fixation of the rotor for damages.
4. Check the rotor’s suitability using the chemical compatibility chart on on page B-1.
5. Make sure the tubes or bottles do not touch the bucket lid.
6. Check the rotor bolts and apply grease (75003786) before using for the first time.
7. Check that each bucket or micro-plate carrier can swing freely by moving it carefully with your
hand. Weigh the bucket content (adapter and tube). Make sure you do not exceed the maximum
compartment load.
Proper Loading
To ensure safe operation of the centrifuge, the rotor must be evenly loaded at all times.
The buckets and micro plate carriers must always be fully loaded with racks. If there are racks missing,
the running racks might fall over and cause a rotor failure due to imbalance.
CAUTION Always be sure to use buckets of the same weight class. Operate the rotor always
with the lid closed.

4 Rotor Loading
Improper Loading
Thermo Scientific 75006480 DiagnostikTM Rotor 4-3
Improper Loading

4 Rotor Loading
Improper Loading
4-4 75006480 DiagnostikTM Rotor Thermo Scientific

Thermo Scientific 75006480 DiagnostikTM Rotor 5-1
5
Maintenance and Care
Contents
•“Cleaning Intervals” on page 5-2
•“Cleaning” on page 5-2
•“Disinfection” on page 5-3
•“Decontamination” on page 5-4
•“Autoklavieren” auf Seite 6-5
•“Service of Thermo Fisher Scientific” on page 5-5

5 Maintenance and Care
Cleaning Intervals
5-2 75006480 DiagnostikTM Rotor Thermo Scientific
Cleaning Intervals
For the sake of personal, environmental and material protection, it is your duty to clean and if
necessary, disinfect the centrifuge on a regular basis.
Cleaning
When cleaning centrifuge mind the following:
•Use warm water with a neutral solvent.
•Never use caustic cleaning agents such as soap suds, phosphoric acid, bleaching solutions or
scrubbing powder.
•Rinse the cavities out thoroughly.
•Use a soft brush without metal bristles to remove stubborn residue.
•Afterwards rinse with distilled water.
•Place the rotors on a plastic grate with their cavities pointing down.
•If drying boxes are used, the temperature must never exceed 50 °C, since higher temperatures
could damage the material and shorten the lifespan of the parts.
•Use only disinfectants with a pH of 6-8.
•Dry aluminum parts off with a soft cloth.
•After cleaning, treat the entire surface of aluminum parts with corrosion protection oil
(70009824). Also treat the cavities with oil.
•Store the aluminum parts at room temperature or in a cold-storage room with the cavities pointing
down.
Maintenance Recommended Interval
Clean Rotor Chamber Daily or when polluted
Clean Rotor Daily or when polluted
Accessories Daily or when polluted
Cabinet Once per Month
Ventilation Holes Every six Months
CAUTION Refrain from using any other cleaning or decontamination procedure than those
recommended here, if you are not entirely sure that the intended procedure is safe for the
equipment.
Use only approved cleansers.
If in doubt, contact Thermo Fisher Scientific.