ThermoFisher Scientific DXR xi User manual

DXRxi Instruments and Software
DXRxi
Raman Imaging Microscope
with OMNICxi Raman Imaging Software
User Guide
269-300706 Revision A September 2021

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The contents of this document are subject to change without notice. All technical information in this
document is for reference purposes only. System configurations and specifications in this document supersede
all previous information received by the purchaser.
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free and assumes no responsibility and will not be liable for any errors, omissions, damage or loss that might
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For Research Use Only. This instrument or accessory is not a medical device and is not intended to be
used for the prevention, diagnosis, treatment or cure of disease.
WARNING Avoid an explosion or fire hazard. This instrument or accessory is not
designed for use in an explosive atmosphere.

Thermo Scientific DXRxi User Guide iii
C
Chapter 1 Introducing the DXRxi Raman Imaging System. . . . . . . . . . . . . . . . . . . . . . . . . . . . .1
About the System . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 1
Getting Started . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 2
Safety and Operating Precautions . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 2
About this Help System. . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 3
Organization . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 3
Conventions . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 4
Contacting Us . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 4
Trademarks . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 5
Chapter 2 DXRxi Microscope Operation . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .7
Microscope Features and Controls . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 7
Front View . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 7
Right Side View. . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 8
Left Side View . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 10
Inside Sample Compartment . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 11
Stage Controls . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 12
Operating the Microscope. . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 13
Indicators . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 13
Powering the System Up or Down . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 14
Loading a Sample . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 14
Available Sample Holders . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 15
Rotating Stage Holder . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 17
Sample Holder Breadboard Kit With Clips . . . . . . . . . . . . . . . . . . . . . . . . 18
XPS Sample Holder . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 18
Raman Macro Sampler. . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 19
Leveling the Sample Holder. . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 19
Installing Objectives . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 23
Configuring Sample Illumination . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 24
Controlling Illumination with the Optional Reflected Light
Brightfield/Darkfield Illuminator . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 24
Controlling Illumination with the Optional Reflected Light Brightfield
Illuminator. . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 26
Controlling Transmitted Light Illumination . . . . . . . . . . . . . . . . . . . . . . . 27
Using Optical Filters . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 27
Contents

Contents
iv DXRxi User Guide Thermo Scientific
Using Optical Polarizers. . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 29
Positioning the Motorized Stage . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 37
Using Confocal Operation . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 38
Using the Raman Polarization Option. . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 38
Changing the Laser, Filter, and Grating. . . . . . . . . . . . . . . . . . . . . . . . . . . . . 39
Installing a 633 nm Laser . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 43
Enabling the Laser. . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 43
Optional Class 3B Laser Configuration . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 43
Using a Class 3B Fiber Optic Launcher Accessory . . . . . . . . . . . . . . . . . . . 44
Installing the Microscope Alignment and Calibration Tool . . . . . . . . . . . . . . 46
Servicing the Microscope. . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 51
Cleaning Your Instrument. . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 51
Replacing the Nosepiece . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 52
Replacing an Illuminator Bulb. . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 52
Safety Information. . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 55
Laser Precautions. . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 55
Biohazards or Radioactive Materials and Infectious Agents . . . . . . . . . . . . . . 56
Cooling Vents . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 56
Ventilation . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 57
Microscope Safety Labels. . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 58
Chapter 3 OMNICxi Software Operation. . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .63
General Workflow. . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 63
Overview of the Workspace. . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 64
OMNICxi Acquisition Window . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 64
General Features and Controls . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 66
Video Mode Features and Controls . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 77
Image Regions Mode . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 82
Image Cross Section Mode. . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 93
Live Spectrum Mode . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 94
OMNICxi Analysis Window. . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 99
3D Analysis and Terrain Windows . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 110
Instrument Settings . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 116
Collect a Mosaic . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 120
Acquire and Analyze Data . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 125
Analyze Regions. . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 126
Acquire a Preview Chemical Image. . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 126
Settings for Acquiring a Chemical Image . . . . . . . . . . . . . . . . . . . . . . . . . 127
Acquire and Analyze a Chemical Image . . . . . . . . . . . . . . . . . . . . . . . . . . 130
Profile Settings . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 131
Reanalyze Image Data . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 143
Identify and Analyze Particles . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 145
Video Image-Based Particle Analysis . . . . . . . . . . . . . . . . . . . . . . . . . . . . 145
Chemical Image-Based Particle Analysis. . . . . . . . . . . . . . . . . . . . . . . . . . 146
Settings for Particle Analysis. . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 146

Contents
Thermo Scientific DXRxi User Guide v
View Particle Analysis Results . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 149
View Spectrum at Specific Location . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 150
Analyze Sample Cross Sections . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 153
Measure Change Over Time . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 159
View Time-Based Data . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 162
Measure and View 3D Volumes . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 163
Acquire 3D Image Data . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 163
View 3D Data . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 164
Verify Instrument Performance . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 164
System Tools . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 155
Aligning and Calibrating Laser Sets . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 155
Creating a New Video Calibration . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 160
Chapter 4 Troubleshooting. . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .171
Problems and Solutions . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 171
Performance Verification fails . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 171
Sample Bands are too Small. . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 172
Sample Bands Are Too Large or CCD Overflowed . . . . . . . . . . . . . . . . . . . 172
No Raman Signal Appears in the Live Display or Spectrum Has No
Sample Bands. . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 172
No Visible Image Appears in the Video Pane or Viewer . . . . . . . . . . . . . . . 173
The Spectrum Has a Sloping Background or a Severe Positive Offset . . . . . 173
The Spectrum Baseline Is Severely Tilted, Curved or Saturating . . . . . . . . . 173
The Video Image is Obscured or Distorted . . . . . . . . . . . . . . . . . . . . . . . . . 174
The Computer Cannot Communicate with the Instrument . . . . . . . . . . . . 174
Chemical Image Appears to be Different Than the Video Image . . . . . . . . . 174
Chemical Image Does Not Achieve Required Spatial Resolution. . . . . . . . . 175
Chemical Image Shows Missing Data . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 175
X-Axis Calibration Fails. . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 175
Error Messages . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 176
General Errors . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 176
Instrument off or disconnected. . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 177
Video camera disconnected . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 177
Stage controller off or disconnected . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 177
Stage controller failed. . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 178
Unknown stage location. . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 178
Detector too warm . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 178
Detector disconnected . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 179
No filter . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 179
No grating . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 179
No laser . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 179
Mismatched grating . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 179
Mismatched filter. . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 179
Invalid fiber probe configuration . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 180
Uncalibrated laser, grating, filter set . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 180

Contents
vi DXRxi User Guide Thermo Scientific
Alignment/calibration out of date. . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 180
Stage firmware out of date . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 180
Sample door open . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 180
Laser off . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 180
Laser key off . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 180
Laser warming up. . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 180
Sample signal too high . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 181
Cannot read instrument EEPROM . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 181
No communication . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 181
Align and Calibration Errors . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 181
Align/calibrate tool not connected . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 182
Laser output too low . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 182
No laser at sample . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 182
Poor quality align/calibrate spectra . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 183
Spectral response too low . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 184
Spectral response too high . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 184
Performance check value(s) out of range. . . . . . . . . . . . . . . . . . . . . . . . . . 185
Misaligned filter or grating. . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 185
Unable to read system info . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 186
Unable to collect spectral data . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 186
Unable to calibrate laser power. . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 186

Thermo Scientific DXRxi User Guide 1
1
Introducing the DXRxi Raman Imaging System
Welcome to the Thermo Scientific™ DXR™xi Raman imaging microscope with OMNIC™xi
Raman imaging software. This system is intended to be used to analyze the chemical
composition of materials using dispersive Raman spectroscopy. This help system will get you
started using the microscope and software and demonstrate how to run experiments using a
provided sample.
This section covers these topics:
About the System
The DXRxi Raman Imaging Microscope combines rapid chemical characterization with
high-end spectral performance. It has interchangeable lasers and their associated filters and
gratings, and accommodates polarizers and various optical filters. The instrument offers
reflected light brightfield sample illumination. Optional reflected light brightfield/darkfield
and transmission illumination kits are also available.
Contents
•About the System
•Getting Started
•Safety and Operating Precautions
•About this Help System
•Contacting Us
•Trademarks
NOTICE Besure thatallpersons operating this system read the site and safety
m anualfirst.

1Introducing the DXRxi Raman Imaging System
Getting Started
2DXRxi User Guide Thermo Scientific
The instrument includes our OMNICxi Raman imaging software for data collection and
analysis. The OMNICxi software comprises two main windows:
•theAcquisition window for data collection and on-the-fly analysis
•theAnalysis window for viewing and processing previously collected image data
With automatic feature recognition and real-time optimization of imaging parameters, this
imaging system is a powerful tool for component identification that will transform the way
you approach materials analysis.
Getting Started
The instrument can be run from a Windows™-compatible computer connected to the
instrument. The computer must meet our requirements for processing speed and memory.
The system is set up and installed by one of our service representatives. For best performance,
leave the power connected and the instrument powered up even when not in use.
The DXRxi Quick Reference guide provides a comprehensive overview of the microscope and
software. If you are using the instrument the first time, start by reviewing the quick reference
guide and completing the steps as you go. A printed copy of the guide is provided with the
system. A PDF version is also available in the electronic documentation set.
Safety and Operating Precautions
The instrument contains precise optical components. Handle it carefully. Before using the
system, read the site preparation and safety manual. A copy in PDF format is included in the
provided documentation set. Always follow the safety and operating precautions in that
manual and in this help system when using the instrument.
The DXRxi Raman imaging microscope may be configured as a Class 1 laser-safe system or
optionally as a Class 3B laser product.
For complete safety information, refer to the DXR Site and Safety Guide. A PDF copy is
included in the electronic documentation set.

1 Introducing the DXRxi Raman Imaging System
About this Help System
Thermo Scientific DXRxi User Guide 3
Follow these additional precautions when using the instrument:
•Avoid pinch hazard. Keep hands and fingers clear of the microscope sampling stage
when the stage is in motion.
•Avoid explosion hazard. Do not use the instrument to analyze flammable or explosive
materials.
About this Help System
•Organization
•Conventions
Organization
This help system has the following main sections:
WARNING Avoid eye injury. The system is a Class 3B laser product emitting visible or
invisible laser radiation when using the fiber optic accessory or operating with the
microscope cover removed. Exposure to laser radiation could cause permanent eye
damage.
• All persons using or in the vicinity of a Class 3B system must wear safety eyewear that
exceeds the specifications for the laser in use
• Do not look directly into a laser, even when wearing laser safety eyewear
• Set up a laser safety area in the location where a Class 3B system is being used
• Making adjustments, using controls, or performing procedures that are not specified
in the documentation could result in exposure to hazardous visible or invisible laser
radiation.
CAUTION Avoid eye injury. Do not use magnets near the instrument. Magnets can defeat
safety interlocks, causing emission of visible and/or invisible laser radiation when
compartment doors on the instrument are open.
Section Description
Introduction Overview of the instrument and this help system plus information
about contacting us and the system warranty
DXRxi Raman
Imaging Microscope
Complete instructions for operating, maintaining and servicing
the microscope

1Introducing the DXRxi Raman Imaging System
Contacting Us
4DXRxi User Guide Thermo Scientific
Conventions
This help system uses the following conventions for safety and special notices:
Contacting Us
For U.S. Technical Support, please contact:
Unity Lab Services
Part of Thermo Fisher Scientific
5225 Verona Road
Madison WI 53711-4495 U.S.A.
Telephone: [phone]
E-mail: [e-mail]
For International Support, please contact:
Thermo Fisher Scientific
Telephone: [phone]
E-mail: [e-mail]
OMNICxi Raman
Imaging Software
Descriptions of all software features and controls
Troubleshooting Troubleshooting information and descriptions of possible error
messages
Section Description
WARNING Indicates a hazardous situation which, if not avoided, could result in death or
serious injury.
CAUTION Indicates a hazardous situation which, if not avoided, could result in minor or
moderate injury.
NOTICE Follow instructions with this labelto avoid dam aging the system
hardware orlosing data.
Note Contains helpful supplementary information.
Tip Provides information that can make a task easier.

1 Introducing the DXRxi Raman Imaging System
Trademarks
Thermo Scientific DXRxi User Guide 5
Trademarks
Microsoft, Windows and Excel are either trademarks or registered trademarks of Microsoft
Corporation in the United States and/or other countries. MATLAB is either a trademark or a registered
trademark of The MathWorks, Inc. in the United States or other countries. All other trademarks are the
property of Thermo Fisher Scientific Inc. and its subsidiaries.
Note Please have the instrument serial number available when you contact us.

1Introducing the DXRxi Raman Imaging System
Trademarks
6DXRxi User Guide Thermo Scientific

Thermo Scientific DXRxi User Guide 7
2
DXRxi Microscope Operation
The DXRxi Raman Imaging Microscope combines rapid chemical characterization with
high-end spectral performance. It has interchangeable lasers and their associated filters and
gratings, and accommodates polarizers and various optical filters. The instrument offers
reflected light brightfield sample illumination. Optional reflected light brightfield/darkfield
and transmission illumination kits are also available.
Contents
•Microscope Features and Controls
•Operating the Microscope
•Servicing the Microscope
•Safety Information

2DXRxi Microscope Operation
8DXRxi User Guide Thermo Scientific

Thermo Scientific DXRxi User Guide 7
Microscope Features and Controls
Front View
Figure 1. Microscope with optional reflected light brightfield/darkfield (BF/DF) illuminator
Sample compartment doors
(lift to open)
Laser power indicator
Power button (hold down
3 seconds to power on or off)
Light path control
Eyepiece
Video
camera
Laser
Grating Laser filter
Sample
area cover
Power indicator
Laser at sample indicator

Microscope Features and Controls
8DXRxi User Guide Thermo Scientific
Right Side View
Figure 2. Microscope with optional reflected light brightfield/darkfield (BF/DF) illuminator
Field Iris control (default all the way in)
Aperture Iris control (default all the way in)
Neutral density filter control
Illumination intensity control
Video camera port
Video cameraEyepiece Optical filter slots (2), insert from other side
Reflected light fixed polarizer insertion slot
View path control
(default half way in/out)
Light path control
Polarization analyzer insertion slot

Microscope Features and Controls
Thermo Scientific DXRxi User Guide 9
Figure 3. Power supply for optional reflected light brightfield/darkfield (BF/DF) illuminator
Figure 4. Microscope with reflected light brightfield only illuminator
Brightness control (for
reflected light only)
Power switch
Power indicator
Illumination intensity control
Video camera cable receptacle
Video cameraEyepiece
Optical filter slot (insert from other side)
Reflected light fixed polarizer
insertion slots
View path control
(default half way in/out)
Polarization analyzer
insertion slot
Eyepiece

Microscope Features and Controls
10 DXRxi User Guide Thermo Scientific
Left Side View
Figure 5. Microscope, left side view with laser keyswitch
Laser keyswitch in locked
position (laser is disabled)
Microscope power
cord receptacle
Grating

Microscope Features and Controls
Thermo Scientific DXRxi User Guide 11
Inside Sample Compartment
Figure 6. Microscope, inside sample compartment
Single slide holder ObjectivesSample stageNosepiece

Microscope Features and Controls
12 DXRxi User Guide Thermo Scientific
Figure 7. Microscope objectives and sample stage (10x objective is included)
Stage Controls
Figure 8. Microscope sample stage controls
Sample stage
Objective
Current objective
must also be selected
in OMNICxi software
Nosepiece
Single slide holder
Joystick (fine x and
y-axis stage control)
Focus control (fine
z-axis stage control)
Stage speed control (hold
down to speed up joystick,
or tap to toggle between
slow, medium and fast)
Focus speed control (hold down
to speed up focus control, or tap
to toggle between slow, medium
and fast)