Tokai Hit STX-E Series User manual

Stage Top Incubator
STX –E series
Instruction Manual
MA-STX-E-EN-01

Index
For safe and comfortable use
・・・・・・・ 3
1
System Structure
・・・・・・・・・・・・・
1.1 Components
7
2 Controller
・・・・・・・・・・・・・・・・
2.1 Size and weight
2.2 Name of parts
2.3 Specifications
8
3 Chamber
・・・・・・・・・・・・・・・・・
3.1 Structure
3.2 Installation
3.3 How to set vessel to Dish Attachment
3.4 How to set Dish Attachment to chamber
3.5 How to set Lens Heater
3.6 How to set Temperature Sensor
3.7 Access Ports
11
4 Connection ・・・・・・・・・・・・・・・・
4.1 System Image
4.2 Connection of Gas Tubes
25
5
Operation ・・・・・・・・・・・・・・・・
5.1 Touch Panel
5.2 Reference Setting Value
5.3 Work-flow
5.4 Preliminary Operation
5.5 Gas supply
5.6 Cultivation
5.7 Cleaning
27
6 Note and Trouble
-shooting ・・・・・・・ 36
7 Options
・・・・・・・・・・・・・・・・・・ 40

Controller
(printed on rear panel)
Chamber
Lens Heater
For safe and comfortable use
About symbols and indications
These warnings enable safe and correct usage, and prevent dangerous
situations. Please follow them at all times.
Symbol meaning
WARNING Indicates a hazardous which could result serious injury.
CAUTION Indicates a situation which could result slight injury.
CAUTION Indicates a situation which could damage the equipment
or other apparatus.
NOTE Point of attention
Indication meaning
Injury or damage to equipment is possible
Must not be done
Must be done
High temperature possible
Electric shock possible
Information
Position of indication
3

To all users
This device is to be used by researchers doing cultivation
observations in a laboratory. It cannot be used at home.
Do not take the equipment apart. This results in damage.
When shipping
Please treat this precision instrument with care. Impacting it is
forbidden.
Do not put any heavy things on the box.
Do not turn the box upside down.
Do not let the unit moisten.
Regarding the environment for usage and storage
Flammable or corrosive gas or oil mist that can damage electrical
insulation
Intense vibration or impact
High voltage power lines, inductive interference
Place where dew drops are present or with direct sunlight
Place with much dust
Do not use this equipment in the below conditions.
Points to pay attention when installing to a microscope
Install the chamber in the state that the condenser is high enough.
There is a risk that the condenser hits and breaks the top glass.
Avoid stress on wires and tubes connected to the chamber.
4

Connect the cylinder to the controller with supplied tube and fasten
with band. Using another tube causes breakage and escape of gas.
If the tube is not fastened well, there is a risk that it comes off.
Do not bend the silicon tubes. If they are plugged, gas cannot be
adequately supplied to the chamber.
Do not use any gas other than specified. Gas leaks, dissolution of
tubes and equipment, and malfunction may be occurred.
Connect gas cylinder to the controller independently. If gas is
blanched off and used with other devices, it may be the cause of
trouble for both the equipment and other devices.
Use only the specified electricity and cables. If other cables are used,
this might result in electric shocks. Using a non-specified voltage
might result in equipment damage.
When using the equipment
Please use this equipment in a large room. If you use the equipment
in a small tightly isolated room, CO2 concentration in air might rise
and adversely affect human bodies.
Do not handle the equipment with wet hands. It might cause
damage to electric equipment.
In the case of glass breakage, do not touch the glass. It might result
in injury and electric shock.
When revolving the objectives, be careful not to hit and damage
both microscope and lens heater by the wire.
Take the following points into consideration in order to avoid focus
drift during long-term microscope observations.
・Keep the room temperature stable at 20℃-28℃.
・Do not let air flow directly over the chamber.
With the exception of bath unit, wipe off media or water quickly. It
can be the cause of damage.
Do not immerse the gas spouting nozzle into water. It may cause
vibration and affect images under observation.
5

To pull out the plug from the outlet swiftly in an emergency, do not
place any things near the outlet.
Storage
Turn off power supply while performing maintenance,
When opening the chamber, do not inhale air in the chamber directly.
It may adversely affect human bodies.
Clean device with soft cloth using small amount of diluted neutral
detergent. Do not use organic solvents.
Do not wash the equipment with water.
After use, make sure to remove all water completely. If some water
remains, this can result in molt, breeding of bacteria and erosion.
Do not use volatile materials such as benzene or thinner for cleaning.
Use of such materials will discolor and/or damage key device
surfaces.
It is important to read this Instruction Manual before using the
equipment. If it is used in manner other than specified in this manual,
safety cannot be assured and it may result in damage to the
equipment which will not be covered under warranty. Please follow
the instructions.
6
Summary
This equipment is an incubation system for research which has the
following characteristics.
Long term observations of cell cultures are possible right on the
microscope stage through reproduction of temperature, humidity and
CO2 needed for cell culture. The temperature regulation is conducted by
Top Heater of Chamber Unit, humidity with heating water in Water Bath.
In order to achieve clear view during observation lid includes glass heater,
even during high humidity no condensation appears.
The Lens Heater prevents heat from escaping to the objective lens under
oil/water immersion.
The chamber is small and compact (stage top type) to be used on the
microscope stage. It does not take much space around the microscope.
The optimal cell-culture environment is created with the controller
equipped with PID control.

1.1 Components
Name of components Qty
Controller * STXG or STXF 1
Chamber 1
Lens Heater 1
Dish Attachment
Note
Fixing Lid
Temperature Sensor 1
Extension Wire for Temperature Sensor 1
Gas Tube 2
Precision Screw Driver 1
Spare coverglass for LX-D35 1
Power Cable １
Disposable Syringe for Draining Water 1
1 System Structure
Note：Accessories differ depending on the product model.
Economy series include following items:
Included Dish Attachment and Fixing lid
ATX-A for ATX-D
ATX-D for 35mm dish and 50/60mm dish
ATX-W for wellplate
LX-D35 for 35mm dish
LX-D56 for 50/60mm dish
LX-W for wellplate
-OPTION-
➀Sample Feedback regulation
②STX-APP: Software
③ATX-CSG for chamber slide, chambered coverglass and slide glass
LX-CSG for chamber slide, chambered coverglass and slide glass
※ Please refer to 7 Options (for STX-E upgrade) for more details.
7

①
②
2.1 Size and weight
The image is shown for reference only.
No. Name Role Note
①Main Switch Power ON/OFF
②Touch Panel Setting and indication For the usage, see P.27-30.
③Flowmeter Indication of gas flow
④Flow Adjustment
Knob Adjustment of gas flow Equipped to STXF
STXG with built-in gas mixer STXF with analog flowmeter
③
2 Controller
②
③
Front panel
2.2 Name of parts
Model Dimension Weight
STXG W151xD263xH196 mm 3.8 kg
STXF W151xD296xH196 mm 3.5 kg
8
①
④

No. Name Role Note
①USB Connection Connection to PC For connection to PC, see P.25.
②Link Connection A Connection to optional gas mixer Use only if the system is used
with STX optional gas mixer.
③Sensor Connection Connection to Extension Wire
④Chamber
Connection Connection to Chamber
⑤Mixed Gas Outlet Gas supply to Chamber
For connection of tubes, see
P.25-26.
⑥CO2Inlet
Intake of
100% CO2（STXG）
5% CO2premixed gas（STXF）
⑦Power Cable
Connection
STXG with built-in gas mixer STXF with flowmeter
Rear Panel
9
①②
⑥
③
④
⑤
⑦
⑥
⑤
④
③
②
⑦
①

STXG
with built-in gas mixer
STXF
with analog flowmeter
Temperature
Temperature control PID control
Increments 0.1℃ step -
Setting method Touch Panel or PC (optional, STX-APP requires)
Possible temp.
setting range
Controllable temp.
setting range is from
ambient +5℃
SAMPLE TEMP 30℃ -40℃ (optional, STX-FB requires)
TOP HEATER 10℃ -65℃
BATH HEATER 10℃ -50℃
STAGE HEATER 10℃ -50℃
LENS HEATER 10℃ -45℃
Rise time 10 minutes to reach 50℃
Accuracy Within ±0.3℃ at each heater surface（in our terms and conditions）
Sensor (for heaters) Pt100
Humi
dity
Humidification control Forced humidification by heating Bath Unit
Condensation prevention Top Heater equipped with glass heater
Gas
CO2concentration range 5% - 20% -
Control method PID control Precision needle valve
Accuracy Within ±0.1% Within ±2% FS
Gas type 100％CO25% CO2premixed gas
Input gas pressure 0.1Mpa - 0.15MPa
Output gas pressure 160 ml/min 75 ml/min - 250 ml/min
Power source 100 V ～240 V AC ±10％50/60Hz
Maximum power consumption 110W 90W
For use in areas with
100V-120V power
Use only Power Cable described below: detachable cord set, 3-conductor grounding
at 125V, AC125V7A minimum, listed in UL. In case of using with extension cord, use
only Power Cable with PE (Protective Earth) wire.
For use in areas with
220V-240V power
Use only 3-pole Power Cable, with plug and outlet complying with EU/EN standards
in EU territory. Class 1 equipment must be connected to PE (Protective Earth)
terminal. In case of using with extension cord, use only Power Cable with PE
(Protective Earth) wire.
Location Indoor
Temperature 20℃-28℃
Relative Humidity 35% -85％
Altitude Up to 2000m maximum
Environmental Conditions Installation category of IEC664, Pollution degree 2
Power Code
Use Conditions
Safety Measures
Burn Prevention Function When the Top Heater exceeds 65℃,
safety function operates and temperature rise stops.
Fuse 250 V AC 2.0A Do not use except the rated fuse.
2.３Specifications
10

3 Chamber
The image is shown for reference only.
No. Name Role Note
①Top Heater ・Condensation prevention
・Sample temperature control
②Bath Unit ・Humidification inside of
chamber by steam
Presence/absence of Bath
Heater depends on the chamber
model.
③Stage Heater
・Heats Dish Attachment
・Prevents heat from escaping
to the stage.
④Lens Heater ・Prevents heat from escaping
the objective lens. For use of Lens Heater, see P.22.
⑤Temperature Sensor ・Measurement of sample temp. Option: Please refer P.41.
⑥Dish Attachment
& Fixing Lid ・Fixation of vessel
Dish Attachment and Fixing Lid
differ depending on the chamber
model.
①
②
③
3.1 Structure
④
⑤
Wellplate
⑥
Objective Lens
Gas Inlet
Sensor/Tube Fixing Block
Water Inlet
WSKMX Chamber
11

WSKMX / IX3WX
3.2 Installation
Applicable vessels
Applicable stage
Flat-top XY stages with 160 x 110 mm aperture
Installable on various stages with optional adapters.
Dimension
W187 ×D146 ×H27 mm
Recommended water volume
32cc
Installation
Fit the bottom part of chamber into the aperture of XY stage.
For use on Prior Scientific H117 stage, see the next page and
remove 3.0mm spacer from the bottom of chamber.
12

Before installing WSKMX/IX3WX on Prior Scientific H117 stage, remove
3.0mm Spacer from the bottom of chamber.
Installation of WSKMX/IW3WX on Prior Scientific H117
①Remove Top Heater (chamber lid).
②Turn the chamber upside down and remove the 4 screws
with Precision Screw Driver that comes with the product.
③Set the chamber without spacer on the H117 stage.
Keep the removed spacer and screws in case of that the system will be
used on other XY stage.
13

WELSX
Applicable vessels
Applicable stage
XY stage with Tokai Hit Stage Adapter or sample holder which can
accommodate wellplate
Dimension
W127.5 ×D85 ×H27 mm
Recommended water volume
21cc
Installation
Fit the bottom part of chamber into the aperture of Stage Adapter
or sample holder.
14

TIZWX
Applicable vessels
Applicable stage
Nikon Ti2/Ti piezo stage
Optional Stage Adapter requires for Ti2/Ti manual/motorized stage.
Dimension
W175.5 ×D118 ×H30 mm 26cc
Installation
Remove Top Heater and use two screws to fix the chamber on
piezo stage or optional Stage Adapter.
15
Recommended water volume

Recommended water volume
TIZBX
Applicable vessels
Applicable stage
Nikon Ti2/Ti piezo stage
Optional Stage Adapter requires for Ti2/Ti manual/motorized stage.
Dimension
W178 ×D118 ×H32 mm 61cc
Installation
Remove Top Heater and use three screws to fix the chamber on
piezo stage or optional Stage Adapter.
16

GSI2X
Applicable vessels
Applicable stage
Leica Super Z galvo stage
Dimension
W126 ×D96 ×H23 mm 18cc
Installation
Remove Top Heater and fix the chamber to stage by four screws on
the side of chamber.
17
Recommended water volume

DMIWX
Applicable vessels
Applicable stage
Leica Scanning stage with condenser cover
Dimension
W170 ×D110 ×H27.5 mm 19cc
Installation
Fit the bottom part of chamber into the aperture of XY stage.
18
Recommended water volume

WSBX
Applicable vessels
Applicable stage
Zeiss Piezo Insert WSB 500
Dimension
W127.5 ×D85 ×H27 mm 18cc
Installation
Fix the chamber to stage by four hexagon socket screws.
19
Recommended water volume

3.3 How to set vessel to Dish Attachment
See the figure below and set vessel.
35 mm dish 50/60 mm dish
Chamber slide and Slide glass(Option) Chambered coverglass(Option)
Use Adapter for 35 mm dish
and fix by magnets of Fixing
Lid LX-D35.
Remove Adapter for 35 mm
dish and fix by magnets of
Fixing Lid LX-D56.
Place the slide on ATX-CSG
and mount Fixing Lid LX-
CSG.
Place the chambered coverglass
and mount Fixing Lid LX-CSG.
Please confirm that the opening
of LX-CSG is completely covered
by chambered coverglass .
ATX-D
Adapter
for 35 mm dish
LX-D35
LX-D56
LX-CSG
ATX-CSG
20