USA Scientific ErgoOne User manual

ADJUSTABLE VOLUME PIPETTE
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English
Table of Contents
ErgoOne · User Manual · 1
Contents
1. Introduction ______________________________________ 2
2. Operation ________________________________________ 3
2.1 Description______________________________________ 3
2.2 Setting the Volume ________________________________ 4
2.3 Pipetting _______________________________________ 5
2.4 Pipette Identification ______________________________ 7
2.5 Safety Notes_____________________________________ 7
3. Troubleshooting __________________________________ 8
4. Cleaning and Decontamination______________________ 9
5. Technical Data ___________________________________ 10
6. Calibration ______________________________________ 11
7. Maintenance_____________________________________ 12
7.1 Maintaining Single-Channel Pipettes __________________ 13
7.2 Maintaining Multi-Channel Pipettes __________________ 16
8. Ordering Information _____________________________ 18
9. Pipette Tip Compatibility__________________________ 20
10. Warranty ________________________________________ 22

2· ErgoOne · User Manual
1. Introduction
At a glance:
•Comfortable ergonomic design
•Easy to use volume adjustment
mechanism
•Volume lock
•Minimal operating forces
•Extremely accurate and precise
•Lightweight
•Low-maintenance high-quality
product
•Fully autoclavable
ErgoOne® variable volume pipettes are
designed in accordance with the latest
ergonomic principles. The pipette is
extremely easy to use and has minimal
operating forces to help prevent hand
fatigue. ErgoOne® provides accurate
and precise pipetting in a variety of
volume ranges and is available in
single-channel, eight-channel and
twelve-channel models.

English
Introduction / Operation
ErgoOne · User Manual · 3
Along with your pipette,
you should also have:
2. Operation
2.1 Description
Color-coded volume control button
with volume adjustment mechanism
Tip ejector
button
Alignment unit
Volume display
Tip ejector
Tip cone
Ejector fixing
TipOne® pipet tips are recommended to
obtain the best results with ErgoOne®.
– Declaration of conformity with
test record
– Labels for personalization/
identification
– Auxiliary tool
(single-channels up to 1ml only)
– Pipette filters (5ml only)

ErgoOne®Volume Ranges
ErgoOne® model Volume range (min./max.) Color code Increments (µl)
2.5µl 0.1–2.5µl Red 0.002
10µl 0.5– 10µl Red 0.01
20µl (micro tip cone) 2– 20µl Red 0.02
20µl (standard tip cone) 2– 20µl Yellow 0.02
100µl 10– 100µl Yellow 0.1
200µl 20– 200µl Yellow 0.2
300µl 30 –300µl Green 0.2
1000µl 100–1000µl Blue 1
5000µl 500–5000µl Violet 5
10µl Pipette
10.00µl 0.50µl 20.0µl 100µl200.0µl 1000µl
200µl Pipette 1000µl Pipette
Examples of typical volume displays:
4· ErgoOne · User Manual
2.2 Setting the Volume
Unlock the volume adjustment catch by pulling
up the volume control button 1(Fig.1), until
you hear a click. Turn the volume control
button 1clockwise to reduce the aspirated
volume, or counter clockwise to increase it.
The set volume is shown in the four-digit
volume display 4from top to bottom. The
white line indicates the decimal point. When
increasing the volume setting, pass the
required value by 1/3 of a turn and then slowly
decrease to reach the volume, making sure not
to go beyond the required volume. After the
volume is set, push the volume control button
1back to the the locked position until you
hear a click. The pipette is now ready to use.
Table 1
Fig.1
Please note the minimum and maximum volume range of your pipette (Table 1).
You have reached the end of the volume adjustment when it becomes more ‘
difficult to turn the button.
Do not use excessive force!

English
Operation
ErgoOne · User Manual · 5
2.3 Pipetting
Use the pipette only when a tip is attached!
Liquid aspiration
Set the required volume (see 2.2 Setting
the volume) and attach the appropriate
size pipette tip(s) to the tip cone(s) 7:
– Press the volume control button 1
until the first stop position.
– Hold the pipette upright and im-
merse approx. 3mm of the pipette
tip into the liquid.
– Allow the volume control button 1
to return slowly back to the starting
position.
– Hold the pipette tip in the liquid for
a further 2 seconds to avoid drawing
in air.
– Remove the pipette tip from the
liquid.
Dispensing fluids
– Place the pipette tip against the
inner wall of the tube or vessel, at an
angle of 30–45°.
– Press the volume control button 1
slowly and evenly until it reaches the
first stop position.
– Wait one second and press the
volume control button 1down to
the second stop position to empty
the pipette tip fully (blow out).
– Keeping the volume control button
1pressed down, lightly draw the
pipette tip up the inner wall of the
tube to remove any residual liquid.
– Allow the volume control button 1
to return slowly back to the starting
position.
– Press the tip ejector button 2to
eject the pipette tip.

6· ErgoOne · User Manual
Reverse pipetting
When pipetting critical viscous media,
the precision and accuracy can be
increased through ‘reverse pipetting’.
In reverse pipetting, a larger volume
is aspirated than that indicated on the
volume display. As a result, a small
amount of residual liquid remains in the
pipette tip after the pipetting process.
How to reverse pipette:
– Secure the pipette tip.
– Press the volume control button 1
until it reaches the second stop
position.
– Hold the pipette upright and
immerse the pipette tip approx.
3mm into the liquid.
– Allow the volume control button 1
to return slowly back to the starting
position.
– Hold the pipette tip in the liquid for
a further 2 seconds to avoid drawing
in air.
– Remove the pipette tip from the
liquid.
– Place the pipette tip against the
inner wall of the tube or vessel, at an
angle of 30–45°.
– Press the volume control button 1
slowly and evenly until it reaches the
first stop position (do not use the
blow-out function).
– Keeping the volume control button 1
pressed, remove the pipette tip from
the tube or vessel before blowing
out the residual liquid into a
separate container.
– Allow the volume control button 1
to return slowly back to the starting
position.
– Press the tip ejector button 2to
eject the pipette tip.
Note: Pre-wetting
Some liquids (eg.solutions containing protein, organic solvents, or viscous liquids)
leave a film on the inside of the pipette tip. To ensure maximum precision and
accuracy, USA Scientific recommends pre-wetting each new tip first by aspirating and
dispensing the liquid two or three times. Another alternative is to use TipOne® RPT
ultra low retention pipette tips. TipOne® RPT tips have optimized surfaces that ensure
improved delivery of detergents, viscous liquids, small volumes, and other difficult
samples. Ask your local USA Scientific representative for more information or go to
www.usascientific.com.