USA Scientific Repeating Pipette User manual

®
®User Manual www.usascientific.com
PO Box 3565
Ocala, FL 34478
Phone: [phone]
352-237-6288
Fax: [phone]
[email protected]
www.usascientific.com
USA Scientific, Inc.
Tolerance Check
Repeating Pipette

3
User Manual 1.
1.1.
2.
2.1.
2.2.
3.
3.1.
3.2.
3.3.
3.4.
4.
5.
6.
7.
8.
9.
10.
General
Design Principle of the Repeating Pipette
Volume Selection and Choice of Tips
®
TipOne Repeating Pipette Tips
Using the 25 and 50 ml Tip Adapters
Operation
Inserting the Tip
Filling the Tip
Dispensing
Removing the Tip
Safety Precautions and Operational Limitations
Troubleshooting Guide
Maintenance, Service and Warranty
Ordering Information
Technical Data
Quick Reference Guide
Manufacturing and Inspection Standards
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3
User Manual 1.
1.1.
2.
2.1.
2.2.
3.
3.1.
3.2.
3.3.
3.4.
4.
5.
6.
7.
8.
9.
10.
General
Design Principle of the Repeating Pipette
Volume Selection and Choice of Tips
®
TipOne Repeating Pipette Tips
Using the 25 and 50 ml Tip Adapters
Operation
Inserting the Tip
Filling the Tip
Dispensing
Removing the Tip
Safety Precautions and Operational Limitations
Troubleshooting Guide
Maintenance, Service and Warranty
Ordering Information
Technical Data
Quick Reference Guide
Manufacturing and Inspection Standards
. . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 4
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Fig.1
The repeating pipette is a hand-held device for
repetitive dispensing. Up to 48 aliquots of the same
sample can be made from a single filling, resulting in
a considerable reduction in time and expense.
The repeating pipette allows the selection of five
separate volumes from each TipOne repeating tip.
Eight different tip sizes provide the most important
volumes from 2 to 5000 µl. Use only original USA
Scientific TipOne repeating tips to guarantee
performance.
1. General
Volume selection dial
To determine the dispensing volume, the dial setting
(1– 5) is multiplied by the minimum aliquot volume
of the TipOne repeating tips.
Dispensing lever
To dispense, press the dispensing lever down until
it stops.
Filling lever
To fill the tip, slide the filling lever upward.
Locking clamp
To secure the tip, tighten the locking clamp down
over the top of the tip.
TipOne repeating pipette tip
1.1. Design Principle of the Repeating Pipette (Fig.1) 2. Volume Selection and
Choice of Tips
4
Find the desired volume in
the columns on the right.
The required dial setting and
maximum number of
pipetting steps for the volume
are shown at the top of the
columns.
Select the corresponding
TipOne repeating tip from
the column on the left.
For repetitive dispensing of a
specific volume:
TIP SELECTION GUIDE
Dispensing Volumes of TipOne Repeating Pipette Tips with the 4736-8025 Repeating Pipette
Selection Dial Setting
Maximum Pipetting Steps
0.1 ml tip
0.5 ml tip
1.0 ml tip
2.5 ml tip
5.0 ml tip
10.0 ml tip
25.0 ml tip
50.0 ml tip
1
48
2 µl
10 µl
20 µl
50 µl
100 µl
200 µl
500 µl
1.0 ml
2
23
4 µl
20 µl
40 µl
100 µl
200 µl
400 µl
1.0 ml
2.0 ml
3
15
6 µl
30 µl
60 µl
150 µl
300 µl
600 µl
1.5 ml
3.0 ml
4
11
8 µl
40 µl
80 µl
200 µl
400 µl
800 µl
2.0 ml
4.0 ml
5
8
10 µl
50 µl
100 µl
250 µl
500 µl
1000 µl
2.5 ml
5.0 ml
*Adapter
necessary
for 25 ml and
50 ml tips
5

Fig.1
The repeating pipette is a hand-held device for
repetitive dispensing. Up to 48 aliquots of the same
sample can be made from a single filling, resulting in
a considerable reduction in time and expense.
The repeating pipette allows the selection of five
separate volumes from each TipOne repeating tip.
Eight different tip sizes provide the most important
volumes from 2 to 5000 µl. Use only original USA
Scientific TipOne repeating tips to guarantee
performance.
1. General
Volume selection dial
To determine the dispensing volume, the dial setting
(1– 5) is multiplied by the minimum aliquot volume
of the TipOne repeating tips.
Dispensing lever
To dispense, press the dispensing lever down until
it stops.
Filling lever
To fill the tip, slide the filling lever upward.
Locking clamp
To secure the tip, tighten the locking clamp down
over the top of the tip.
TipOne repeating pipette tip
1.1. Design Principle of the Repeating Pipette (Fig.1) 2. Volume Selection and
Choice of Tips
4
Find the desired volume in
the columns on the right.
The required dial setting and
maximum number of
pipetting steps for the volume
are shown at the top of the
columns.
Select the corresponding
TipOne repeating tip from
the column on the left.
For repetitive dispensing of a
specific volume:
TIP SELECTION GUIDE
Dispensing Volumes of TipOne Repeating Pipette Tips with the 4736-8025 Repeating Pipette
Selection Dial Setting
Maximum Pipetting Steps
0.1 ml tip
0.5 ml tip
1.0 ml tip
2.5 ml tip
5.0 ml tip
10.0 ml tip
25.0 ml tip
50.0 ml tip
1
48
2 µl
10 µl
20 µl
50 µl
100 µl
200 µl
500 µl
1.0 ml
2
23
4 µl
20 µl
40 µl
100 µl
200 µl
400 µl
1.0 ml
2.0 ml
3
15
6 µl
30 µl
60 µl
150 µl
300 µl
600 µl
1.5 ml
3.0 ml
4
11
8 µl
40 µl
80 µl
200 µl
400 µl
800 µl
2.0 ml
4.0 ml
5
8
10 µl
50 µl
100 µl
250 µl
500 µl
1000 µl
2.5 ml
5.0 ml
*Adapter
necessary
for 25 ml and
50 ml tips
5

Number
of Pipetting
Steps
Position
of Volume
Selection Dial
48 1
23 2
15 3
11 4
8 5
Fig. 2 Fig. 3 Fig. 4 Fig. 5 Fig. 6
76
®
2.1. TipOne Repeating Pipette Tips
The minimum dispensing volume and the maximum
filling capacity is printed on each tip (Fig. 2). The scale
facilitates drawing off partial quantities and helps you to
estimate the amount of liquid still available for operation.
The volume to be dispensed is obtained by multiplying the
number selected on the volume selection dial by the
minimum dispensing volume of the tip.
For example, the 1.0 ml tip has a minimum volume where
1=20 μl. At setting 4, the dispensed volume will be 4 x 20 μl
or 80 μl.
For increased precision, choose a small capacity tip and
use a high dial setting. The correlation between the
position of the volume selection dial and the number of
dispensing steps is as shown in the table.
2.2. Using the 25 and
50 ml Tip Adapters
(Fig.3)
Attach the adapter to the
25 ml or 50 ml tips by
turning it clockwise; remove
by turning it counter
clockwise.
Slide the filling lever
down until it stops.
Raise the locking clamp
upward.
Insert the tip until it
clicks into position. The
tip plunger must be
completely inside the tip
barrel before attaching it
to the repeating pipette
(Fig. 4).
3. Operation
3.1. Inserting the Tip
With the filling lever in
the down position,
lower the locking
clamp until it clicks to
secure the tip in place
(see Fig. 5).
3.2. Filling the Tip
Use the following steps for
all sizes of tips. 0.1 ml,
0.5 ml, 1.0 ml, and 2.5 ml
tips may also be used with
a standard 200 µl pipette
tip. Attaching a tip
facilitates filling from
narrow-necked vessels or
piercing of sealing foil and
also makes dispensing of
small volumes more
precise.
Immerse the tip into the
liquid.
Slowly slide the filling
lever upward (Fig. 6).
Discard the liquid from
the first dispensing
step. The repeating
pipette is now ready for
operation.

Number
of Pipetting
Steps
Position
of Volume
Selection Dial
48 1
23 2
15 3
11 4
8 5
Fig. 2 Fig. 3 Fig. 4 Fig. 5 Fig. 6
76
®
2.1. TipOne Repeating Pipette Tips
The minimum dispensing volume and the maximum
filling capacity is printed on each tip (Fig. 2). The scale
facilitates drawing off partial quantities and helps you to
estimate the amount of liquid still available for operation.
The volume to be dispensed is obtained by multiplying the
number selected on the volume selection dial by the
minimum dispensing volume of the tip.
For example, the 1.0 ml tip has a minimum volume where
1=20 μl. At setting 4, the dispensed volume will be 4 x 20 μl
or 80 μl.
For increased precision, choose a small capacity tip and
use a high dial setting. The correlation between the
position of the volume selection dial and the number of
dispensing steps is as shown in the table.
2.2. Using the 25 and
50 ml Tip Adapters
(Fig.3)
Attach the adapter to the
25 ml or 50 ml tips by
turning it clockwise; remove
by turning it counter
clockwise.
Slide the filling lever
down until it stops.
Raise the locking clamp
upward.
Insert the tip until it
clicks into position. The
tip plunger must be
completely inside the tip
barrel before attaching it
to the repeating pipette
(Fig. 4).
3. Operation
3.1. Inserting the Tip
With the filling lever in
the down position,
lower the locking
clamp until it clicks to
secure the tip in place
(see Fig. 5).
3.2. Filling the Tip
Use the following steps for
all sizes of tips. 0.1 ml,
0.5 ml, 1.0 ml, and 2.5 ml
tips may also be used with
a standard 200 µl pipette
tip. Attaching a tip
facilitates filling from
narrow-necked vessels or
piercing of sealing foil and
also makes dispensing of
small volumes more
precise.
Immerse the tip into the
liquid.
Slowly slide the filling
lever upward (Fig. 6).
Discard the liquid from
the first dispensing
step. The repeating
pipette is now ready for
operation.

Additional Information
Fig. 7 Fig. 8
Raising the filling lever too quickly can cause an
excessive vacuum. This may cause tiny air bubbles
to accumulate in the liquid, resulting in inaccurate
volumes.
Important: The first dispensing step must be
discarded so that any residual filling pressure may
be released and the system is prepared for precise
delivery. Small bubbles in the tip beneath the piston
will not affect the accuracy since the residual stroke
lock prevents the delivery of any remaining sample
after the last dispensing step.
It is not necessary to completely fill the tip. Partial
filling will not cause dispensing inaccuracies.
98
3.3. Dispensing (Fig.7)
Check the volume
selection dial.
Wipe the end of the tip with
lint-free tissue.
Hold the tip so it touches
the inner wall of the vessel.
Dispense the liquid by
completely depressing the
dispensing lever.
Additional Information
It is not necessary to attach a standard 200 µl pipette
tip to the 5.0 ml or 10.0 ml repeating tips. Never
attach a standard pipette tip when working with the
25 ml or 50 ml repeating tips.
A new tip must be used each time to avoid carry-over
and contamination.
3.4. Removing the Tip (Fig.8)
Hold the repeating pipette over a beaker.
Slowly depress the filling lever down to the stop.
This will completely empty the tip.
Raise the locking clamp upward.
Remove the tip.
Note: 25 ml or 50 ml tips are removed from the
reusable adapter by turning counter clockwise.
4. Safety Precautions and Operational Limitations
When removing the repeating pipette tips, please note the following:
Do not touch the tip, especially when using hazardous reagents!
Empty the tips into a proper disposal bin to avoid splashing and
aerosols.
Treat any retained fluid as a bio-hazard. Do not allow the fluid to
come into contact with laboratory personnel.
If a liquid with a surface tension significantly different from water
is being dispensed, inaccuracies or leaking can occur.
The repeating pipette with an attached, partially filled tip should
be stored in a stand to prevent air from entering the pipette.

Additional Information
Fig. 7 Fig. 8
Raising the filling lever too quickly can cause an
excessive vacuum. This may cause tiny air bubbles
to accumulate in the liquid, resulting in inaccurate
volumes.
Important: The first dispensing step must be
discarded so that any residual filling pressure may
be released and the system is prepared for precise
delivery. Small bubbles in the tip beneath the piston
will not affect the accuracy since the residual stroke
lock prevents the delivery of any remaining sample
after the last dispensing step.
It is not necessary to completely fill the tip. Partial
filling will not cause dispensing inaccuracies.
98
3.3. Dispensing (Fig.7)
Check the volume
selection dial.
Wipe the end of the tip with
lint-free tissue.
Hold the tip so it touches
the inner wall of the vessel.
Dispense the liquid by
completely depressing the
dispensing lever.
Additional Information
It is not necessary to attach a standard 200 µl pipette
tip to the 5.0 ml or 10.0 ml repeating tips. Never
attach a standard pipette tip when working with the
25 ml or 50 ml repeating tips.
A new tip must be used each time to avoid carry-over
and contamination.
3.4. Removing the Tip (Fig.8)
Hold the repeating pipette over a beaker.
Slowly depress the filling lever down to the stop.
This will completely empty the tip.
Raise the locking clamp upward.
Remove the tip.
Note: 25 ml or 50 ml tips are removed from the
reusable adapter by turning counter clockwise.
4. Safety Precautions and Operational Limitations
When removing the repeating pipette tips, please note the following:
Do not touch the tip, especially when using hazardous reagents!
Empty the tips into a proper disposal bin to avoid splashing and
aerosols.
Treat any retained fluid as a bio-hazard. Do not allow the fluid to
come into contact with laboratory personnel.
If a liquid with a surface tension significantly different from water
is being dispensed, inaccuracies or leaking can occur.
The repeating pipette with an attached, partially filled tip should
be stored in a stand to prevent air from entering the pipette.

5. Troubleshooting Guide
Tip cannot be inserted.
Unable to fully raise the filling lever.
Excessive air bubbles develop during filling.
Inconsistent dispensing.
Tip drips.
The repeating pipette jams or does not function.
The locking clamp cannot be fully raised unless the
filling lever is completely down.
The tip piston is not completely inserted in the barrel.
Locking clamp incompletely depressed.
Filling lever slightly lifted before immersing into the liquid.
The tip is leaking.
Volume selection dial is not set into position.
The tip is leaking.
The tip is not tightly attached.
Depress the filling lever downward and raise the
locking clamp as far as it will go.
Slide the piston into the proper position.
Completely depress the locking clamp.
Draw a small amount of solution into the tip and
discard it completely. Refill the tip.
Replace the tip with a new one.
Turn the volume selection dial into position until it clicks.
Replace the tip with a new tip.
Use more force to attach the tip.
Call 800-522-8477 or 352-237-6288 for assistance.
Error Cause Solution
1110
6. Maintenance, Service and Warranty
External surfaces of the pipette can be cleaned with
warm water or isopropyl alcohol.
There are no other maintenance requirements.
Important: Under no circumstances should the pipette
be dismantled!
Do not autoclave the pipette or the tips!
If a problem cannot be solved with the aid of the
Troubleshooting Guide, call USA Scientific at
800-522-8477 or 352-237-6288 for repair or
replacement.
Important: Decontamination of the pipette will be required
before a return. We will send you a decontamination
form to complete.
Please contact USA Scientific for more detailed
information.
Please check that the unit has been received complete
and undamaged. Save all packaging and documents
until you have thoroughly inspected your shipment; if you
find that your order is incorrect or damaged please call
USA Scientific.
This warranty is valid for 24 months with the condition
that the product has been used and cared for according
to this User Manual. No liability is accepted for loss or
damage arising from incorrect use.
The liability is limited to the repair or replacement of the
unit. USA Scientific is not liable for any consequential
damages. We refer to our general terms of sale.
USA Scientific reserves the right to alter these
specifications without prior notice.
Maintenance Service Warranty