Vitrolife EmbryoScope User manual

EmbryoScope™time-lapse incubator
User manual
EmbryoScopetime-lapse incubator, ES-D2, software version 7.7
User manual, first issue 2020.04.24, revised 2022.06.01/EU/EGS/English

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Table of contents
1Introduction..........................................................................................................................5
1.1 Warnings, restrictions and limited warranty ................................................................6
1.2 Important notes ........................................................................................................10
1.3 Intended use –EmbryoScope time-lapse incubator..................................................12
1.4 Intended use –EmbryoSlide culture dish..................................................................12
1.5 Intended users..........................................................................................................12
1.6 Clinical benefits........................................................................................................12
2Overview of the EmbryoScope time-lapse incubator......................................................13
2.1 Installation and required conditions ..........................................................................14
2.2 Starting up the EmbryoScopetime-lapse incubator ..................................................15
2.3 Shutting down the EmbryoScope time-lapse incubator.............................................15
3Restarting the integrated PC.............................................................................................16
4Connections to supporting systems................................................................................17
5The incubator.....................................................................................................................18
5.1 The incubator control panel......................................................................................18
5.1.1 Overview of menu items accessible from the control panel.........................18
5.2 Locking or unlocking the incubator control panel......................................................19
5.3 Temporarily pausing audible warning alarms............................................................20
5.4 Incubator temperature..............................................................................................20
5.4.1 Changing the temperature set point............................................................20
5.4.2 Validating the temperature..........................................................................20
5.4.3 Calibrating the temperature.........................................................................22
5.5 Incubator CO2concentration.....................................................................................24
5.5.1 Changing the CO2set point ........................................................................24
5.5.2 Validating the CO2concentration................................................................25
5.5.3 Calibrating the CO2 concentration............................................................... 26
5.6 Incubator O2concentration.......................................................................................27
5.6.1 Changing the O2set point...........................................................................27
5.6.2 Validating the O2concentration...................................................................27
5.6.3 Calibrating the O2concentration .................................................................28
6Operating the EmbryoScope time-lapse incubator.........................................................29
6.1 Start-up procedure....................................................................................................29
6.2 Resetting and calibrating the camera .......................................................................31
6.3 Starting an EmbryoSlide culture dish........................................................................36
6.3.1 Associating an existing patient with a new EmbryoSlide culture dish ..........37

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6.3.2 Creating a new patient on the EmbryoScope time-lapse incubator .............38
6.4 Playing a time-lapse video........................................................................................39
6.5 Refocusing embryos.................................................................................................40
6.6 Ending an EmbryoSlide culture dish.........................................................................40
7Routine checks of the EmbryoScope time-lapse incubator............................................42
7.1 Starting validation checks.........................................................................................42
7.2 Gas check................................................................................................................42
7.3 Temperature check...................................................................................................44
7.4 Cleaning check.........................................................................................................45
8Cleaning the EmbryoScope time-lapse incubator...........................................................46
8.1 Periodic cleaning of the EmbryoScope time-lapse incubator ....................................46
8.2 Disinfection of the EmbryoScope time-lapse incubator.............................................47
9Alarm and information signal system ..............................................................................50
9.1 Warning alarms........................................................................................................51
9.1.1 Control unit responses to warning alarms...................................................51
9.1.2 Overview of warning alarms........................................................................53
9.1.3 Multiple warning alarms..............................................................................54
9.2 Incubator malfunction alarms....................................................................................54
9.2.1 Control unit responses to an incubator malfunction alarm...........................55
9.2.2 Operator response to an incubator malfunction alarm.................................56
9.3 Technical alarms......................................................................................................56
9.3.1 Control unit responses to technical alarms..................................................56
9.3.2 Operator response to technical alarms........................................................56
9.4 Information signals ...................................................................................................57
9.4.1 Malfunction information signals................................................................... 57
9.4.1.1 Control unit responses to malfunction information signals...........58
9.4.2 Warning information signals........................................................................59
9.4.2.1 Control unit responses to warning information signals ................60
9.5 External alarm system..............................................................................................61
9.5.1 Delay of external alarms and malfunction information signals.....................62
9.5.2 Connecting the external alarm....................................................................63
9.5.3 Normally open and normally closed circuits................................................64
10 Emergency procedure.......................................................................................................65
10.1 Removing EmbryoSlide culture dishes after a power failure.....................................65
10.2 Removing EmbryoSlide culture dishes after a system failure....................................65
11 Technical specifications....................................................................................................67
12 EMC and HF technical review ...........................................................................................71

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12.1 Electromagnetic emissions.......................................................................................71
12.2 Electromagnetic immunity.........................................................................................72
13 Accessories and materials................................................................................................76
14 Planned service and maintenance....................................................................................77
15 Symbols and labels ...........................................................................................................78
15.1 Product information labels........................................................................................78
15.2 Warning labels..........................................................................................................79
15.3 Connection labels.....................................................................................................80
16 Disposal of waste...............................................................................................................81
17 Contact information...........................................................................................................82
CohortView, CulturePro, EmbryoScope, EmbryoSlide, EmbryoViewer, Guided Annotation,
iDAScore and KIDScore are trademarks or registered trademarks belonging to the Vitrolife Group.
©2022 Vitrolife A/S. All rights reserved.

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1 Introduction
This user manual provides information on how to use the EmbryoScope time-lapse incubator
Version D (hereafter EmbryoScope time-lapse incubator).
A person certified by Vitrolife will perform scheduled maintenance and recalibration checks
according to a service plan to ensure continued safe and efficient operation. The end user is
strongly encouraged to follow the service plan carefully to ensure error-free operation of the
equipment.
The EmbryoScopetime-lapse incubator is a medical device that must be operated by trained
personnel according to the instructions contained in this user manual. Users must be qualified to
operate the device and qualified to perform procedures associated with device use in accordance
with local qualification standards.
The product fulfils the requirements of the UL 60601-1 edition 1 and IEC 60601-1:2005 + corr. 1
(2006) + corr. 2 (2007) standards; class I, type B equivalent. The EmbryoScope time-lapse
incubator is suitable for continuous operation.
•The EmbryoScope time-lapse incubator and related accessories conform to the
requirements of Regulation (EU) 2017/745 on medical devices, classified as class IIa.
•Conforms to ANSI/AAMI ES 60601-1:2005 + A1 + A2.
•Certified to CAN/CSA - C22.2 No. 60601-1:R2013 addendum.

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1.1 Warnings, restrictions and limited warranty
Users must agree to read and understand this user manual and observe the safety instructions
before operating the EmbryoScope time-lapse incubator.
RESTRICTIONS ON USE
•The EmbryoScope time-lapse incubator may only be used by persons who have been
trained in its use by a person certified by Vitrolife.
•The EmbryoScope time-lapse incubator may only be used with sterile disposable
EmbryoSlide culture dishes produced and sold by Vitrolife.
•The EmbryoSlide culture dishes may not be reused.
•The EmbryoSlide culture dishes must be covered with sterile lids before insertion into the
EmbryoScope time-lapse incubator.
•The EmbryoScope time-lapse incubator may not be used in a wet environment. No fluids
other than culture medium and oil and the cleaning agents specified in this user manual
may be used in or near the incubator.
•Never cover the vent holes on the back of the incubator in part or in whole as this may
cause the incubator to overheat.
•Users should contact Vitrolife immediately to report any incident and/or injury to a patient,
operator or maintenance employee that occurred as a result of operating the
EmbryoScope time-lapse incubator. Any serious incident that has occurred in relation to
the incubator should be reported to the competent authority of the Member State in which
the user is established.
•Should an accident occur while using the EmbryoScope time-lapse incubator, stop using
the time-lapse incubator until it has been checked by a person certified by Vitrolife.

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WARNING
•The EmbryoScope time-lapse incubator includes moving parts with safety stops. Do not
try to block safety sensors by inserting a finger or a hand into the EmbryoScope time-
lapse incubator while it is turned on. This is dangerous and may cause injury.
•To avoid risk of electric shock, this equipment must only be connected to a mains supply
with a protective earth, which is connected to a residual-current device (RCD) or ground
fault (circuit) interrupter (GFI/GFCI).
•The EmbryoScope time-lapse incubator should always be connected to a local
uninterruptible power supply (UPS) to ensure stable operating conditions in case of
power failure.
•Portable and mobile RF communications equipment may affect the EmbryoScope time-
lapse incubator.
•Mishandling or misuse of the EmbryoScope time-lapse incubator may result in serious
injury to the user.
•The EmbryoScope time-lapse incubator is not suitable for use in the presence of a
flammable anaesthetic mixture with air or with oxygen or nitrous oxide.
•It is the user’s responsibility to validate the performance of the EmbryoScope time-lapse
incubator by performing regular quality control checks on the temperature, CO2and O2*
levels.
* Only if the clinic incubates with reduced O2concentration.
•During initial start-up and after an incubator shutdown, always validate the gas and
temperature levels using calibrated external validation devices as detailed in the
EmbryoScope user manual. Do NOT rely solely on values displayed on the incubator
control panel.

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INSTALLATION AND MAINTENANCE
•Installation and repair of the EmbryoScope time-lapse incubator may only be carried out
by a person certified by Vitrolife. The EmbryoScope time-lapse incubator must remain at
the location where it was installed. If an EmbryoScope time-lapse incubator is
disconnected and/or moved without supervision by a person certified by Vitrolife, the
EmbryoScope time-lapse incubator will no longer be approved for clinical use and the
warranty may be voided.
•If the EmbryoScope time-lapse incubator or parts of it are modified, appropriate
inspection and testing must be conducted by a person certified by Vitrolife to ensure
continued safe use.
•When cleaning and disinfecting the EmbryoScope time-lapse incubator, always use the
prescribed chemical agents as specified in section 8 of the EmbryoScope user manual.
TRANSPORTATION AND RELOCATION OF THE EMBRYOSCOPE TIME-LAPSE
INCUBATOR
•While the EmbryoScope time-lapse incubator is still in the shipping boxes, it must only be
moved by using a lift truck or a pallet lifter. Do NOT open the shipping boxes without the
presence of a person certified by Vitrolife.
•When the EmbryoScope time-lapse incubator has been unpacked, it must only be moved
by two persons supporting the device underneath the left and right sides and only under
the supervision of a person certified by Vitrolife.
CONNECTION TO EXTERNAL EQUIPMENT
(EN 60601-1 MEDICAL ELECTRICAL EQUIPMENT –PART 1)
•External equipment intended for connection to signal input, signal output or other
connectors must comply with the relevant IEC standard (i.e. EN 60601-1:2006 –Part 1
for medical electrical equipment). In addition, all such combinations –systems –must
comply with the standard EN 60601-1:2015 –Part 2, General requirements for basic
safety and essential performance. Equipment not complying with EN 60601-1:2006 –
Part 1 must be kept outside the patient environment, i.e. at least 1.5 m from the patient or
the patient support.
•Any person who connects external equipment to signal input, signal output or other
connectors has formed a system and is therefore responsible for ensuring that the
system complies with the requirements of EN 60601-1:2006 –Part 1. If in doubt, contact
a qualified medical technician or your local representative.

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ELECTROMAGNETIC COMPABILITY
•The EmbryoScope time-lapse incubator has been tested and found to comply with the
limits for medical devices set out in the IEC 60601-1-2 Edition 4.0 for electromagnetic
compatibility. These limits are designed to provide reasonable protection against harmful
interference in a typical medical installation.
Compliance with IEC 60601-1-2 Edition 4.0 ensures compatibility when the EmbryoScope
time-lapse incubator is placed at a minimum distance to nearby instruments. If the
EmbryoScope time-lapse incubator is placed close to other instruments, it is necessary to
observe that the performance of all instruments remains unaffected by the placement.
The EmbryoScope time-lapse incubator generates, uses and can radiate radio frequency
energy and, if not installed and used in accordance with the instructions, may cause
harmful interference to other devices in the vicinity. However, there is no guarantee that
interference will not occur in a particular installation. If this equipment does cause harmful
interference to other devices, which can be determined by turning the equipment off and
on, the user is encouraged to try to correct the interference by one or more of the
following measures:
a) Reorient or relocate the receiving device.
b) Increase the separation between the equipment.
c) Connect the equipment to an outlet on a circuit different from that to which the other
device(s) are connected.
Consult the manufacturer, its representative or its dealer for help.
WARNING
•The use of accessories, transducers and cables other than those specified, with the
exemption of transducers and cables sold by the manufacturer of the system as
replacement parts for internal components, may result in increased emissions or
decreased immunity of the equipment or system.
•The EmbryoScope time-lapse incubator should not be used adjacent to or stacked with
other equipment. If adjacent or stacked use is necessary, the incubator should be
observed to verify normal operation in the configuration in which it will be used.
CONFIDENTIALITY
•All identification numbers, names and treatment data presented in this manual are
fictional.

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LIMITED WARRANTY
•Vitrolife warrants the EmbryoScope time-lapse incubator to be free from defects in
materials and workmanship for a period of one (1) year from the first installation date.
The limited warranty will terminate immediately if installation, maintenance, repair or
relocation of the time-lapse incubator is carried out by other than Vitrolife-certified
personnel.
The limited warranty does not apply to damage resulting from:
a) failure to perform routine maintenance in accordance with the EmbryoScope user
manual;
b) accident, abuse, misuse or misapplication of the time-lapse incubator;
c) use and operation that does not comply with instructions provided in the
EmbryoScope user manual; or
d) normal wear and tear.
GENERAL CYBER SECURITY RECOMMENDATIONS
•Users are advised and expected to take the following measures to reduce cyber security
risk in order to ensure that the device will work as designed in the intended user
environment:
oEnsure that personnel are properly trained in cyber security awareness
oPrevent physical access to the equipment by unauthorised users.
•Users must inform Vitrolife A/S without any undue delay upon becoming aware of a cyber
security vulnerability incident or any suspected security events.
1.2 Important notes
NOTE –EXACT INSEMINATION TIME
•When a new EmbryoSlide culture dish is inserted, it is important to enter the exact
insemination time and date as all subsequent events such as cell divisions will be related
to the time of insemination. Entering an incorrect time and date will thus compromise the
quality of your data.

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NOTE –AUTO-FOCUS PROCEDURE
•If the auto-focus procedure did not find the best focused image, you can manually
refocus the images by selecting the Live View option (home screen -> select the relevant
EmbryoSlide culture dish by pressing the Slide tab -> press the image of the relevant
well -> press the Live button).
NOTE –LIVE VIEW
•The automatic image acquisition is stopped in Live view during refocusing, adjustment of
focal planes or adjustment of exposure time. The image acquisition will automatically
restart after 30 minutes if you leave the EmbryoScope time-lapse incubator inactive in
Live mode. The time period until the image acquisition is automatically resumed will be
30 minutes, even if you have set up the EmbryoScope time-lapse incubator to produce
images every 10 minutes. You may, however, manually restart the image acquisition
without any delay by returning the EmbryoScope time-lapse incubator to the embryo
overview screen.
•If you adjust any of the camera settings in Live mode, the new settings are universally
applied to all wells and EmbryoSlide culture dishes.
•When you have opened the Live view without working actively on the screen for two
minutes, the light will automatically be turned off to prevent the embryo from being
excessively exposed to the light. In this case, a red note will appear on the screen with
the following text: “Camera light has been turned off. Press any key or button to re-
activate”.
NOTE –PAUSING EMBRYOSLIDE CULTURE DISHES
•While an EmbryoSlide culture dish is paused, no time-lapse images are acquired from the
remaining EmbryoSlide culture dishes.
•If an EmbryoSlide culture dish has been paused for more than one hour, the
EmbryoScope time-lapse incubator will issue an alarm. This audible alarm can be
temporarily paused by pressing Reset alarm on the incubator touch screen.

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NOTE –DATA STORAGE
•When you have more than 5 GB free storage space, files older than 50 days are
automatically deleted from the Scope Data folder.
•When you have less than 5 GB free storage space, your hard drive is starting to fill up. In
this case, ALL files older than seven days will be deleted. The oldest files will be deleted
first until the amount of free storage space is back to more than 5 GB.
•ALL of the data are stored either on the PC running the EmbryoViewer software or on the
ES server from which they will not be deleted.
1.3 Intended use –EmbryoScope time-lapse incubator
The intended use of the EmbryoScope incubator is to provide an environment with controlled
temperature and gas concentrations (CO2and optionally O2) for the culture of gametes and/or
embryos and to acquire images of these during incubation.
For an overview of the EmbryoScope time-lapse incubator, see section 2.
1.4 Intended use –EmbryoSlide culture dish
The EmbryoSlide culture dish is an accessory for embryo storage related to the EmbryoScope
incubator, which provides an environment with controlled temperature and gas concentrations
(CO2and optionally O2) for the development of gametes and/or embryos at or near body
temperature.
For a detailed description of the EmbryoSlide culture dish, refer to the user manual for the
EmbryoSlide culture dishes.
1.5 Intended users
Embryologists, other laboratory personnel and clinic staff at IVF clinics trained by Vitrolife A/S-
certified instructors.
1.6 Clinical benefits
•Improved embryo development
•Improved implantation/pregnancy rate
•Reduced pregnancy loss rate.

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2 Overview of the EmbryoScope time-
lapse incubator
Image series are analysed automatically in real time with proprietary software. Blastomere activity
is a numerical parameter that reflects the amount of movement that has occurred between two
consecutive frames in the time-lapse image series. The blastomere activity has NO DIAGNOSTIC
USE, but can be used to aid users in identifying areas in the time series where events of interest
may be occurring. No operator input is required, and the output is available at any time during
incubation.
Reset button
(visible when
the keyboard is
pulled out)
Incubator control
panel
Load door lock
Pull-out keyboard
USB socket
Load door
Touch screen
Backplate. Contains main
switch, connectors and
sockets
Service lid. Access to gas sample
pipe and filters. A copy of the
emergency procedures is also found
under the service lid

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2.1 Installation and required conditions
The incubator must be set up in accordance with the installation checklist. It should not be moved
or disconnected by persons who are not certified by Vitrolife.
Installation requirements:
•Clean room with a stable temperature between 20°C and 30°C.
•Sturdy table with approximately 1.0 x 0.6 m of bench space.
•A minimum distance of 150 mm between the back wall of the EmbryoScope time-lapse
incubator and any solid object.
•Optional: uninterruptible power supply (UPS), min. 300 W, with proper grounding.
•Residual-current device (RCD) or ground fault (circuit) interrupter (GFI/GFCI).
•Specification of attachment plug for connection to the alternate voltage: NEMA 5-15
(hospital grade).
•CO2gas supply with pressure regulator capable of providing stable output of CO2 between
0.6 –1 bar above ambient.
•N2gas supply with pressure regulator capable of providing stable output of N2between
0.6 –1 bar above ambient.
•Medical electrical equipment requires special precautions regarding EMC and must be
installed and put into service according to the EMC information provided.
NOTE
•No cooling facilities are present in the embryo chamber. If the temperature rises above
the specified limits, the temperature inside the embryo chamber will therefore reach at
least ambient temperature.
•It is strongly recommended, although not required, to connect the incubator to an
uninterruptible power supply (UPS) with earth connector to ensure stable operating
conditions in case of power failure. Any UPS connected to the EmbryoScope incubator
must comply with the following directives and harmonised standards:
oLow Voltage Directive 2014/35/EU
oElectromagnetic Compatibility Directive 2014/30/EU
oEN 62040-1:2009 Uninterruptible power systems (UPS) –Part 1: General and safety
requirements for UPS
oEN 62040-2:2006 Uninterruptible power systems (UPS) –Part 2: Electromagnetic
compatibility (EMC) requirements.

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2.2 Starting up the EmbryoScopetime-lapse incubator
The EmbryoScope time-lapse incubator should be turned on at least three hours before use to
ensure temperature equilibration throughout the time-lapse incubator. Please ensure that the
EmbryoScope time-lapse incubator is grounded through the power connector, that the gas
connections are not leaking, and that the gas reservoir is full. Furthermore, you should periodically
check the residual pressure of the gas cylinders and replace the CO2or N2cylinders if the pressure
drops below 40 bars. The back pressure in the connecting tubes should not exceed 1 bar or drop
below 0.6 bar.
2.3 Shutting down the EmbryoScope time-lapse incubator
In order to shut down the EmbryoScope time-lapse incubator (e.g. for service or cleaning), follow
the procedure described below.
NOTE
•In case of an emergency, please follow the procedure described in section 10.
1. Remove all EmbryoSlide culture dishes from the EmbryoScope time-lapse incubator (see
the procedure in section 6.6).
2. Press the Shutdown button on the home screen.
3. Select Close Computer in the presented dialogue box. This turns off the PC of the
EmbryoScope time-lapse incubator (the incubator itself will continue to run).
4. Press OK in the presented dialogue box.
5. In order to turn off the incubator, use the main power switch located at the back of the
EmbryoScope time-lapse incubator. See also section4.
NOTE
•If the EmbryoScope time-lapse incubator is left with the desktop open for an extended
period of time, the software embedded in the incubator is not running and no time-lapse
images will be acquired. In this case, the incubator will activate a warning information
signal.
NOTE
•The software of the EmbryoScope time-lapse incubator and Microsoft Windows should
always be shut down correctly before the power is switched off.

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3 Restarting the integrated PC
In some cases, it will be necessary to restart the PC that is integrated in the EmbryoScope time-
lapse incubator, e.g. if an error condition occurs.
To restart the PC:
1. Pull out the keyboard at the bottom of the EmbryoScope time-lapse incubator.
2. Use a pointed object such as a pencil or a ballpoint to press the small red button available
to the right of the keyboard (see the illustration in section 2). The PC will now shut down.
3. Press the red button again to restart the PC.

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4 Connections to supporting systems
All connectors and sockets are found at the back of the EmbryoScopetime-lapse incubator.
Connectors and sockets should only be used by a person certified by Vitrolife during installation.
Operators should never use or attach any tubing/wiring to the panel.
Product information label.
See section 15.1
Ethernet connection socket
CO2connection inlet
Service readout socket
Manufacturer
contact
information.
See section 17
External alarm
output socket.
See section 9.5
N2connection
inlet
Power supply socket
Fuse
replacement
information.
See section 11
Main power switch

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5 The incubator
5.1 The incubator control panel
The incubator control panel is located in the upper left corner of the EmbryoScope time-lapse
incubator:
The control panel is used for:
•Pausing audible warning alarms issued by the EmbryoScope time-lapse incubator (using
the pause audio button)
•Inspecting a range of incubation parameters such as temperature, CO2 concentration, O2
concentration, etc. (from the set point menu)
•Changing the set point of the incubation parameters (from the set point menu)
•Calibrating the EmbryoScope time-lapse incubator (from the calibration menu)
•Turning on or off CO2and O2regulation (from the calibration menu).
5.1.1 Overview of menu items accessible from the control panel
The incubator control panel provides access to two separate menus: the set point menu and the
calibration menu.
Overview of menu items available from incubator control panel

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To navigate from the set point menu to the calibration menu, press the down arrow for about three
seconds. To navigate back from the calibration menu to the set point menu, press the up arrow for
about three seconds. While the menu is being changed, the display will flash:  until
the change of menu is complete.
To navigate back and forth between the items available in each menu, press the left or right arrow.
For example, if the temperature is currently displayed in the set point menu and you want to
inspect the CO2concentration, press the right arrow once. If you afterwards want to return to the
temperature, press the left arrow once.
5.2 Locking or unlocking the incubator control panel
The control panel can be locked to ensure that no parameters are inadvertently changed. When
the control panel is locked, you cannot change the set point or calibrate the parameters; neither
can you turn on or off CO2or O2regulation.
If you attempt to change any of the parameters when the panel is locked, the display will show:
to indicate that a lock is active and needs to be removed before the action can be
performed.
To lock the control panel:
Simultaneously press and hold the SP button and the left arrow for about three seconds:
The display will briefly show: to indicate that the control panel has been locked.
To unlock the control panel:
Simultaneously press and hold the SP button and the right arrow for about three seconds:
The display will briefly show: to indicate that the control panel has been unlocked.
+
+

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5.3 Temporarily pausing audible warning alarms
To pause the audible alarm signal for three minutes, press the pause audio button: . Pausing
the warning alarm will not affect the LED, which will continue to flash until the problem that caused
the warning alarm has been solved.
5.4 Incubator temperature
5.4.1 Changing the temperature set point
1. In the set point menu, make sure that the display shows the incubator temperature. If this is
not the case, navigate to the temperature menu item by pressing the right or left arrow
until the current temperature set point is displayed: .(temperature menu
item –the set point is: 37°C).
2. Press the button. The display will start to alternate between showing .
(temperature menu item –the set point is: 37°C) and .(set point menu is active
–the set point is: 37°C). This indicates that you are about to adjust the temperature set
point.
3. While still holding the button, press the up or down arrow to increase or
decrease the set point. Each time you press the up or down arrow once, you will either
increase or decrease the set point by 0.1°C.
4. Release the button to save the new setting.
5.4.2 Validating the temperature
The EmbryoScope time-lapse incubator must have been turned on for at least three hours before
the temperature is validated. The room temperature must be equivalent to normal laboratory
temperature. The temperature validation is performed by measuring the temperature in the culture
dish holder using a temperature probe connected to a high-precision thermometer.
NOTE
•While performing this procedure, be very careful not to inadvertently touch or push the
EmbryoSlide culture dish(es) present in the culture dish holder.
•Before you start, make sure that the high-precision thermometer is calibrated according
to the manufacturer’s specifications.