Walz PAM-2500 User manual

Printed in Germany
2.156/11.2019
Second Edition, November 2019
PAM_2500_06.doc
Heinz Walz GmbH, 2019
Heinz Walz GmbH · Eichenring 6 · 91090 Effeltrich · Germany
Phone [phone] · Telefax [phone]
[email protected] · www.walz.com
PAM-2500
Portable
Chlorophyll Fluorometer
Handbook of Operation

CONTENTS
1
Contents
1Safety Instructions.......................................................................7
1.1 General Safety Instructions......................................................... 7
1.2 Special Safety Instructions..........................................................7
2Introduction..................................................................................9
2.1 Intention of this Handbook .......................................................10
2.2 Important points........................................................................11
3Components and Setup..............................................................13
3.1 Basic System Components........................................................13
3.2 Basic System Setup...................................................................13
3.3 PamWin-3 Software Installation...............................................15
3.3.1 Software Update...............................................................17
3.4 Accessories ...............................................................................17
3.4.1 Field Combo (optional).....................................................17
3.4.2 Additional Components....................................................19
4PAM-2500 Operation.................................................................29
4.1 PamWin-3 Help.........................................................................29
4.2 Field Screen ..............................................................................29
4.2.1 Monitoring Graphs............................................................30
4.2.2 Program and Script Control..............................................32
4.2.3 Alphanumeric Area...........................................................33
4.2.4 First Measurements Using the Field Screen.....................43
4.3 PamWin-3: Advanced Level.....................................................46
4.3.1 General Settings................................................................46
4.4 SP-Analysis Mode.....................................................................61
4.4.1 Slow Kinetics Window.....................................................61

CONTENTS
2
4.4.2 Light Curve....................................................................... 64
4.4.3 Report ............................................................................... 68
4.5 Fast Acquisition Mode.............................................................. 72
4.5.1 Fast Settings...................................................................... 73
4.5.2 Fast Kinetics..................................................................... 82
4.6 View Mode ............................................................................... 87
4.6.1 Icon Bar ............................................................................ 87
4.6.2 Sidebar.............................................................................. 87
4.6.3 Pick Data........................................................................... 87
4.7 Script Files................................................................................ 89
4.7.1 Data Management............................................................. 91
4.7.2 Editing Tools .................................................................... 91
4.7.3 Command Box (List of Script File Commands)............... 93
4.8Practical tips for creating trigger patterns ........................ 107
4.9Writing scripts....................................................................... 108
5Definitions and Equations....................................................... 115
5.1 Relative Fluorescence Yields ................................................. 115
5.1.1 Measurements with dark-acclimated samples................ 115
5.1.2 Measurements with light-exposed (treated) samples...... 115
5.2 Fluorescence Ratio Parameters............................................... 116
5.3 Constant Fraction of Fo Fluorescence (C/Fo) ........................ 120
5.4 Relative Electron Transfer Rate (ETR).................................. 122
5.5 Rapid Light Curves................................................................. 123
5.5.1 Some Papers related to Rapid Light Curves................... 126
5.6 Literature Cited in Chapter 5.................................................. 127
6Some Reviews on Chlorophyll Fluorescence......................... 130
7Specifications............................................................................ 133

CONTENTS
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7.1 Basic System........................................................................... 133
7.1.1 General Design ...............................................................133
7.1.2 Light sources...................................................................134
7.1.3 Special Fiberoptics 2010-F.............................................135
7.1.4 Distance Clip 60° 2010-A...............................................135
7.1.5 Leaf-Clip Holder 2030-B................................................135
7.1.6 Battery Charger MINI-PAM/L .......................................136
7.1.7 External Voltage Supply Cable MINI-PAM/AK............136
7.1.8 System Control and Data Acquisition ............................136
7.1.9 Carrier Bag......................................................................137
7.1.10 Transport Box 2040-T ....................................................137
7.1.11 Minimum Computer Requirements ................................137
7.2 Accessories .............................................................................137
7.2.1 Touchscreen tablet computer for Field Research...........137
7.2.2 External battery 000160101314......................................138
7.2.3 Automatic charger 000190101099..................................138
7.2.4 Leaf-Clip Holder 2030-B................................................138
7.2.5 Arabidopsis Leaf Clip 2060-B........................................139
7.2.6 Dark Leaf Clip DLC-8....................................................139
7.2.7 Mini Quantum/Temperature-Sensor 2060-M.................139
7.2.8 Suspension Cuvette KS-2500 .........................................140
7.2.9 Magnetic Stirrer MKS-2500...........................................140
7.2.10 Compact Tripod ST-2101A............................................ 140
8Trouble Shooting......................................................................141
8.1 Battery replacement ................................................................142
9Guarantee .................................................................................143
9.1 Manufacturer’s Guarantee ......................................................143

CONTENTS
4
9.2 Conditions............................................................................... 143
9.3 Instructions.............................................................................. 144
9.4 Applicable law........................................................................ 145
10 Index.......................................................................................... 147

CONTENTS
5

SAFETY INSTRUCTIONS CHAPTER 1
7
1Safety Instructions
1.1 General Safety Instructions
a ) Read the safety instructions and the operating instructions
prior to operation of the device.
b ) Pay attention to all the safety warnings.
c ) Keep the device away from water or high moisture areas.
d ) Keep the device away from dust, sand and dirt.
e ) Do not put the device near sources of heat.
f ) Always ensure there is sufficient ventilation.
g ) Connect the device only to the power source indicated in the
operating instructions or on the device. If the device is not in
use, remove the mains plug from the socket
h ) Clean the device only according to the manufacturer’s rec-
ommendations.
i ) Ensure that neither liquids nor other foreign bodies can get
inside the device.
j ) The device should only be repaired by qualified personnel.
1.2 Special Safety Instructions
The PAM-2500 is a highly sensitive instrument which should be only
used for research purposes, as specified in this manual. Follow the
instructions of this manual in order to avoid potential harm to the us-
er and damage to the instrument.
The PAM-2500 can emit very strong light! In order to avoid harm to
your eyes, never look directly at the fiberoptics end, or at open light
ports on the front side of the control unit.

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