Walz MINI-PAM-II User manual

2.163/04.2018
3. Edition, May 2018
MINI_PAM_II_03.docx
Heinz Walz GmbH, 2018
Heinz Walz GmbH •Eichenring 6 •91090 Effeltrich •Germany
Phone [phone] •Telefax +49-(0)9133/5395
E-mail [email protected]om •Internet www.walz.com
MINI-PAM-II
Manual for
Standalone Use

Contents Chapter 1
C1
1 Contents
1 Contents .............................................................. 1
2 Safety Instructions.............................................. 1
2.1 General Safety Instructions ............................................ 1
2.2 Special Safety Instructions.............................................. 1
3 Introduction ......................................................... 3
3.1 Overview............................................................................... 4
4 Components and Setup ...................................... 7
4.1 Extent of Delivery (Basic System) ................................ 7
4.2 Optoelectronic Unit............................................................ 8
4.2.1 Batteries.............................................................................. 11
4.3 2010-A Distance Clip 60° .............................................. 11
4.4 Accessories........................................................................ 13
4.4.1 2035-B Leaf-Clip Holder ................................................ 13
4.4.2 DLC-8 Dark Leaf Clip...................................................... 16
4.4.3 2060-B Arabidopsis Leaf Clip....................................... 18
4.4.4 2060-A Fiberoptics Holder for Surfaces.................... 18
4.4.5 2065-M Mini Quantum/Temp.-Sensor....................... 19
4.4.6 2054-L External LED Source ....................................... 20
4.4.7 MINI-SPEC/MP Miniature Spectrometer .................. 22
4.4.8 KS-2500 Suspension Cuvette...................................... 27
4.4.9 Oxygen Package.............................................................. 27
4.4.10 BCS-9590 Barcode Scanner........................................ 28
4.4.11 MINI-PAM/F1 Miniature Fiberoptics........................... 29
4.4.12 MQS-B/A Adapter Set for Thin Fiberoptics.............. 30
5 Saturation Pulse Analysis ................................ 31
5.1 Five Fluorescence Levels.............................................. 31
5.1.1 Measurements with Dark-Acclimated Samples...... 32
5.1.2 Measurements with Illuminated Samples................. 32

Chapter 1 Contents
C2
5.2 Fluorescence Ratio Parameters ..................................36
5.3 Relative Electron Transfer Rate (ETR)......................38
5.4 Light Curves .......................................................................39
5.5 Some Light Curve References .....................................42
5.6 Some Reviews on Saturation Pulse Analysis..........43
6 Hints & Troubleshooting ...................................45
6.1Instrument Settings..........................................................45
6.2 Default settings .................................................................45
6.3 F0Fluorescence ...............................................................46
6.5 Signal Noise .......................................................................48
6.6 System Hangs ...................................................................48
6.7 External PAR Sensor is not Responding ..................48
6.8 Intensity of external lamp cannot be adjusted.........48
7 Touchscreen Operation.....................................49
7.1 Calibration...........................................................................49
7.2 Top Level Windows .........................................................50
7.2.1 Basic Data ..........................................................................52
7.2.2 Primary Data ......................................................................55
7.2.3 Quenching Analysis.........................................................56
7.2.4 Ft-Chart................................................................................57
7.2.5 Spectrometer .....................................................................58
7.2.6 Actinic + Yield ....................................................................60
7.2.7 Induction Curve.................................................................61
7.2.8 Light Curve .........................................................................64
7.2.9 Recovery .............................................................................64
7.2.10 Actinic Light List ................................................................66
7.3Main Menu..........................................................................70
7.3.1 PAM Settings .....................................................................70
7.3.1.1 Meas. Light.........................................................................71
7.3.1.2 Meas. Light Sett................................................................72
7.3.1.3 Gain ......................................................................................74

Contents Chapter 1
C3
7.3.1.4 Damping.............................................................................. 74
7.3.1.5 ETR-Factor ........................................................................ 75
7.3.1.6 Fo’ Mode............................................................................. 75
7.3.1.7 Adjust F-Offset.................................................................. 75
7.3.2 Light Sources .................................................................... 77
7.3.2.1 Far Red Sett. ..................................................................... 78
7.3.2.2 Light Panel Sett................................................................ 78
7.3.2.3 SAT Settings...................................................................... 79
7.3.3 Program/Clock Settings ................................................. 80
7.3.3.1 Actinic + Yield.................................................................... 81
7.3.3.2 Induction Curve ................................................................ 82
7.3.3.3 Light Curve......................................................................... 83
7.3.4 Sensors ............................................................................... 85
7.3.4.1 Internal PAR ...................................................................... 86
7.3.4.2 Leaf Clip/Ext. PAR........................................................... 88
7.3.4.3 Oxygen Sensor................................................................. 92
7.3.4.4 Spectrometer..................................................................... 93
7.3.4.5 Load System Settings..................................................... 96
7.3.5 MINI-PAM-II Settings...................................................... 96
7.3.6 Memory ............................................................................. 101
7.3.7 Info...................................................................................... 101
7.3.7.1 MINI-PAM-II Info............................................................. 102
7.3.7.2 Sensor Info...................................................................... 103
7.3.7.3 Firmware Info ................................................................. 104
8 Specifications MINI-PAM-II ............................. 105
8.1 Basic System................................................................... 105
8.1.1 Optoelectronic Unit........................................................ 105
8.1.2 Fiberoptics MINI-PAM/F .............................................. 107
8.1.3 Power Supply MINI-PAM-II/N..................................... 107
8.1.4 Battery Charger 000190101101................................ 108
8.1.5 Distance Clip 60° 2010-A ............................................ 108

Chapter 1 Contents
C4
8.1.6 Complementary Items...................................................108
8.1.7 Software WinControl-3..................................................108
8.1.8 Transport Case MINI-PAM/T ......................................109
8.2 Accessories......................................................................109
8.2.1 2035-B Leaf-Clip Holder...............................................109
8.2.2 Fiberoptics Adapter 90° 2030-B90............................110
8.2.3 2054-L External LED Source......................................110
8.2.4 Dark Leaf Clip DLC-8 ....................................................111
8.2.5 Arabidopsis Leaf Clip 2060-B.....................................111
8.2.6 Fiberoptics Holder for Surfaces 2060-A..................112
8.2.7 Mini Quantum/Temp.-Sensor 2065-M .....................112
8.2.8 Miniature Spectrometer MINI-SPEC/MP.................113
8.2.8.1 Flat Entrance Optics SPEC/P.....................................113
8.2.8.2 Fluorescence and Reflection Optics SPEC/R .......113
8.2.8.3 PAR Calibration Block 000160101439 ....................114
8.2.9 Suspension Cuvette KS-2500 ....................................114
8.2.10 Magnetic Stirrer with Fiberoptics Holder MKS-2500
..............................................................................................115
8.2.11 Compact Tripod ST-2101A..........................................115
8.2.12 MINI-PAM/F1 Miniature Fiberoptics .........................115
9 Warranty ...........................................................117
9.1 Conditions.........................................................................117
9.2 Instructions .......................................................................118
10 Index .................................................................119

Safety Instructions Chapter 2
1
2 Safety Instructions
2.1 General Safety Instructions
- Read safety instructions and the operating instructions
prior to operation of the device and its accessories.
- Pay attention to all safety warnings.
- Keep device and its accessories away from water or
high moisture areas.
- Keep the device and its accessories away from dust,
sand and dirt.
- Do not put the device and its accessories near sources
of heat.
- Ensure that neither liquids nor foreign bodies get inside
the device or its accessories.
- Ensure sufficient ventilation.
- Connect the device only to the power source indicated
in the operating instructions or on the device. If the de-
vice is not in use, remove the mains plug from the sock-
et.
- The device and its accessories should only be repaired
by qualified personnel.
2.2 Special Safety Instructions
- The MINI-PAM-II is a highly sensitive instrument which
should be only used for research purposes, as specified

Chapter 2 Safety Instructions
2
in this manual. Follow the instructions of this manual in
order to avoid potential harm to the user and damage to
the instrument.
- The MINI-PAM-II can emit very strong light! In order to
avoid harm to your eyes, never look directly into the light
port of the MINI-PAM-II or its fiberoptics.
- Switch off MINI-PAM-II before connecting or disconnec-
tion 2054-L External LED Source.

Introduction Chapter 3
3
3 Introduction
- The “Photosynthesis Yield Analyzer MINI-PAM-II” has been
designed for highly sensitive saturation pulse analysis of
photosystem II (PS II) in the field as well as in the laboratory.
The automatically calculated parameters are FV/FM
(maximum photochemical yield), Y(II) (effective photochemi-
cal yield) and its complementary yields Y(NPQ) and Y(NO),
as well as parameters of photochemical (qL, qP) and non-
photochemical quenching (qN, NPQ) (see Table 3, page 34).
- The instrument continues the tradition of the preceding MINI-
PAM chlorophyll fluorometer. The major technical advance-
ments of the MINI-PAM-II are the consistent use of energy-
efficient LEDs, an internal PAR sensor, and stand-alone op-
eration by a touchscreen which is well readable under natu-
ral light conditions. Also, a far red LED has been added for
selective photosystem I excitation.
- A further technical progress is the newly designed leaf clip
sensor (2035-B) which measures photosynthetically active
radiation (PAR) at leaf level with high accuracy and, thus,
provides reliable light intensity data for calculations of elec-
tron transport rates (ETR).
- A variety of add-ons make the MINI-PAM-II a highly versatile
measuring system which can be configured to meet the
needs of the research goal. The range of accessories in-
cludes a multi-colored lighting unit (Section 4.4.6, page 20),
an optical oxygen sensor (Section 4.4.9, page 27) and a
miniature spectrometer (Section 4.4.7, page 22).
- For long-term field campaigns, the memory capacity has
been upgraded to keep data of more than 27,000 saturation

Chapter 3 Introduction
4
pulse analyses. The fluorometer is powered by of-the-shelf
AA (Mignon) batteries which are easily replaceable even un-
der field conditions. One set of batteries lasts for up to 1000
saturation pulse analyses.
- The MINI-PAM-II can be operated in the stand-alone mode
or by the well-proven WinControl-3 software. WinControl-3
has been introduced with the JUNIOR-PAM fluorometer and
now operates many other fluorometers like the DIVING-
PAM, MONITORING-PAM, and WATER-PAM. In addition to
the features available in the stand-alone mode, the software
allows evaluations of light-response curves by a non-linear
fitting routine and automatic execution of custom-designed
experiments using the built-in batch file feature.
3.1 Overview
The MINI-PAM-II fluorometer provides a vast range of settings
and protocols for measuring fluorescence. To make full use of
these opportunities, it is necessary to become acquainted with
terminology and principles of saturation pulse analysis. There-
fore, the present manual provides a chapter dealing with the ba-
sics of saturation pulse analysis (Chapter 5, page 31).
Chapter 5 also provides a short list of review papers on PAM
chlorophyll fluorescence and saturation pulse analysis (page 43).
Further, a small section is included providing some hints for be-
ginners (Chapter 6, page 45).
In the field, the MINI-PAM-II is mostly operated in the stand-
alone mode by its touchscreen. Chapter 7 (page 49) provides de-
tailed instructions on how to use the touchscreen interface in-
cluding advices on fluorescence induction and light curve pro-
grams.