Wealtec SpectroArt 200 User manual

SpectroArt 200
Spectrophotometer
Installation and Operation Manual
Version 1.0
Item# 03070
*This instrument is intended for laboratory use only

SpectroArt 200 V1.0
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Index
A. Important Notice ---------------------------------------------------------------- 1
A-1. Warranty
A-2. Technical and Service Contact
A-3. Safety Notice
A-3-1. Certification
A-3-2. Safety Information
B. Introduction ---------------------------------------------------------------------- 3
B-1. Specifications
B-2. Product Description
B-2-1. SpectroArt 200 Spectrophotometer Hardware Overview
C. Installation of SpectroArt 200 Spectrophotometer ------------------ 7
C-1. Package List
C-2. Environment requirement
C-3. SpectroArt 200 spectrophotometer setup
C-3-1. Calibration and Wavelength Linearity Test
C-3-2. UV-Parameters
C-3-3. Touch Pad Settings
C-3-4.PrinterTest
C-3-5. Set Default
C-3-6. Beep Settings
C-3-7. Time/Date Settings
C-3-8. Display Settings
D. Quick Guide to Assays ----------------------------------------------------- 13
D-1. DNA/RNA

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D-2. Protein
D-3. Kinetics
D-4. OD600
D-5. Wavelength
D-6. Scan
D-7. Functions
D-7-1. DNA/RNA
D-7-2. Protein
D-7-3. Kinetics
D-7-4. OD600
D-7-5. Wavelength
D-7-6. Scan
D-7-7. Functions
E. Care and Maintenance -------------------------------------------------------- 22
F. Order Information -------------------------------------------------------------- 25

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A. Important Notice
Before setting up and operating the instruments of SpectroArt 200 spectrophotometer,
please carefully read these instructions to get familiarized with the installation and operation
process. Instructions should be read by experienced individuals before operating the
instruments.
Any improper usage of the instruments may cause damage. Please refer to the safety
notice included with this equipment.
The instruments shall not be modified or altered in any way. Any modification or alteration
will void the warranty, void the regulatory certifications and create potential safety hazard.
Wealtec is not responsible for any injury or damage caused by using the instruments for any
non-intended purpose or injury as a result of modification of the instruments by any person who
is not authorized by Wealtec Corp.
A-1. Warranty
SpectroArt 200 spectrophotometer is warranted to be free from defects in materials or
workmanship for a period of one year from the original invoice date, under normal usage. Any
defects occurring during warranty period, Wealtec Corp. will repair or replace defective products
or parts without charge unless the defects arise from conditions outlined below. The defects
described below are specially excluded from Wealtec warranty policy.
1. Improper operation of the instrument.
2. Repair or modification by any person who is not authorized by Wealtec Corp.
3. Damage caused by any (in)-direct accident, neglect or misuse.
4. Use fittings or spare parts supplied by anyone other than Wealtec Corp.
5. Damage caused by disaster.
6. Damage caused by any improper solvents or samples
A-2. Technical and Service Contact
Most of the operation details are described in this instruction manual to assist and guide
operator for an appropriate solution. For any other technical/ service questions, please contact
your local representative or contact Wealtec international technical/ service specialist by E-mail:
[e-mail].

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A-3. Safety Notice
A-3-1. Certification
The SpectroArt 200 spectrophotometer is designed to meet the international electrical
safety standards EN61010-1 and EMC regulations. This product meets CE requirements and if
operated according to the guidance of the instruction manual, is certified safe. It is possible that
emissions from this product may interfere with some sensitive appliances when placed nearby or
in the same circuit as those applications. The user should be aware of this potential and take
appropriate measures to avoid interference. Any modification or alteration will void the warranty,
void the regulatory certifications and create potential safety hazards.
A-3-2. Safety Information
Before operating the unit, please note the following precautions:
¾Do not use near flammable materials.
¾Always inspect the SpectroArt 200 spectrophotometer for damaged components
before use.
¾Always connect the system to the correctAC power source
¾Always connect the correct instrument (Computer only) via the serial port
connector.
¾Do not pour liquid into the sample chamber. Thorough clean-up is needed after
each spill (Section E. Care and Maintenance).
¾Do not place object on the SpectroArt 200 spectrophotometer.
¾The SpectroArt 200 spectrophotometer has no customer-serviceable components,
do not open the unit.

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B. Introduction
Spectroscopy is a technique that measures the interaction of molecules in a sample with
electromagnetic radiation. The energy of the light produced by the spectrometer of the
spectrophotometer is used to excite molecules in the sample. For every molecule excited, one
photon of the emitted light is absorbed, hence the transmitted light looses some of its energy.
The absorbance of a solute depends linearly on its concentration and is strongly dependant
upon the wavelength of light. This is why spectrophotometry is performed using monochromatic
light, in which all photons have the same wavelength. A spectrum is obtained when the
absorption of light is measured as a function of its wavelength. The light source of SpectroArt
200 is a xenon flash light. In contrast to traditional light sources such as deuterium or
tungsten-halogen that emit either UV-light or visible light receptively, xenon flash lights emit light
of both UV-and visible light wavelengths.
The concentration of a solute can be calculated by measuring either the light that is
absorbed by the sample or the light that is transmitted through the sample. The absorbance (A)
is related to the intensity of light before (I0) [Incident light] and after (I) [Transmitted light] passage
through the solute;
⎟
⎟
⎠
⎞
⎜
⎜
⎝
⎛
−=
o
ll
A10
log
According to Lambert-Beer’s law, the absorbance depends linearly on the concentration of the
solute, where c is the molar concentration, l is the optical path length in cm and εis the molar
absorption coefficient in L mol-1cm-1;
clA
ε
=
% Transmittance can be calculated as;
100*10100% cl
o
ll
T
ε −
=
⎟
⎟
⎠
⎞
⎜
⎜
⎝
⎛
=
The shows the relationship
between Transmittance and
Concentration andAbsorbance and
Concentration.

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B-1. Specifications
Light source Xenon flash light
Detector Photodiode array
Display Touch screen TFT LCD 5.7”
Wavelength range (nm) 200 – 800
Wavelength accuracy (nm) ± 0.5 from 240 – 640
Absorption/Photometric accuracy (nm) ± 0.001@1AU
Absorption/Photometric repeatability (nm) ± 0.005@1AU
Stray light (nm) 0.1%@220
Photometric noise 0.001@0AU, 550
Spectral bandwidth (nm) < 3
Absorbance range (AU) 0 – 3.0
Data collection speed (msec) 60
Path length (mm) 10
Beam length [Z-axis] (mm) 8.5
Pre-set wavelength 9 Max.
Sample test repetition 10 Max.
System optimization On start up
LCD sleep mode Yes
Standard curve storage 10 Max.
Analytic data storage 99 Max.
Kinetic analysis reading interval range (sec) 2 – 999
Communication port RS232
Number of cuvette 1
Applicable cuvette types Standard/Semi-micro/Micro-volume
Printer Built-in
Dimension [LxWxH] (cm) 39 x 29 x 12
Weight (kg) 5
Input 90 – 264 Vac, 47 – 63 Hz
Operating temperature (˚C)
0 – 40
Humidity : 10% to 90% R.H.
Non-condensing

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B-2. Product Description
B-2-1. SpectroArt 200 Spectrophotometer Hardware Overview
Inte
g
rated thermal
p
rinte
r
Touch screen TFT LCD
Cuvette chamber
Light path axis
Thermal printer paper
Printer paper shaft
Sam
p
le com
p
artment lid
Printer paper compartment lid
Prog./Diagnostic
switch Power input
module
Serial output
port (RS-232)
Fan guard
Power switch
Prog. mode
Diagnostic mode

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Display
Touch screen high resolution TFT display.
Sample compartment lid
Lift the sample compartment lid to insert cuvette into the cuvette chamber.
Integrated thermal printer
Built-in alphanumeric/graphic printer. Press print button to record results immediately.
Printer paper compartment lid
Open the printer paper compartment lid to install new roll of printer paper.
Fan guard
Exhaust air leaves cabinet through this opening.
Universal power input module
Appropriate power cord is connected to the power input module.
Power switch
Instrument main power switch.
Serial output port
RS-232 connection to a computer.
Cuvette chamber
Insert cuvette into the cuvette chamber. Insure that the cuvette is placed in the proper
orientation with respect to the light path (Light path axis = Horizontal – x-axis).

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C. Installation of SpectroArt 200 Spectrophotometer
C-1. Package List
Item Quantity
SpectroArt 200 spectrophotometer
1
Thermal paper roll
1
Power cable
1
Instruction and operation manual
1

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C-2. Environment requirement
To insure correct operation and stable performance over an extended period of time, install the
SpectroArt 200 in a location which meets the following conditions:
•Recommended room temperature.
•Not exposed to direct sun light.
•Not subject to direct or continuous vibration.
•Not subject to intense magnetic or electromagnetic fields.
•Relative humidity between 10 – 90 %.
•Area free from corrosive gases or other corrosive substances.
•Area with very little dust or other airbone particles.
•Allow a 15 cm minimum space around the instrument for proper air flow.
C-3. SpectroArt 200 Spectrophotometer Setup
SpectroArt 200 allows you to perform the following in the setup menu
•Calibration
•Adjusting UV parameters
•Restoring touch pad settings
•Printer testing
•Beep volume adjustments
•Time and Date settings
•Display settings (brightness and contrast adjustments)
•Set default settings
To enter the setup menu, touch the “Setup”-button in the main menu
C-3-1. Calibration and Wavelength Linearity Test
A calibration kit is required and can be bought from Wealtec for calibration and wavelength
linearity test. Make sure all the standard samples are wiped off thoroughly with lint free tissues
prior to measurements
•Touch the “Calibration”-button. Make sure no sample is in the cuvette holder and then
touch “OK”.
•Insert the calibration standard into the cuvette holder and then touch “OK”.

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•Touch “OK” to print calibration data.
•Make sure at least six peaks and readings are generated, otherwise, remove sample,
clean and reinsert to repeat calibration procedure.
•Exit the setup menu by touching the “Exit”-button.
•Touch the “Scan”-button in the main menu.
•Remove the calibration standard and touch the “Blank”-button in the scan menu. Make
sure no sample is in the cuvette holder, then touch “OK”.
•Insert the calibration standard once more into the cuvette holder, and then touch
“Sample”. Touch “OK”.
•Print out the graph generated with peaks specified by touching the “Print”-button.
•Check the peaks generated. The detected peak values should always be within ± 3 nm
from the reference peaks
640 nm, 536 nm, 485 nm, 452 nm, 416 nm, 361 nm, 278 nm & 240 nm
•Touch the “Exit”-button to leave the scan menu.
•To perform a wavelength linearity test, touch the “Protein”-button in the main menu.
•Touch the “Setup”-button to enter the protein setup menu.
•Touch the “Method” button five times for customised assay. Then touch the “Change W.
length”-button and key in 280 nm using the number pad.
•Touch the “Blank Rep.”-button and enter the number 5 using the number pad. Then
touch “Enter”.
•Touch the “STD Rep.”-button and enter the number 5 using the number pad. Then touch
“Enter”.
•Touch the “Unknown Rep.”-button and enter the number 5 using the number pad. Then
touch “Enter”.
•Touch the “Exit”-button to leave the protein setup menu and enter the protein menu.
•Touch the “Blank”-button. Ensure no cuvette is in the sample compartment, and then
touch “OK”.
•Touch the “STD” button and enter the unit µg/ml. Press “OK”.
•Insert the blank sample of the standard curve reference and touch “OK”.
•Repeat the procedure 4 times.
•When the display shows “STD CONC. 1/5”, insert the first sample, that is 20 µg/ml. Enter
20, using the number pad. Touch “Enter” and then “OK”.
•Repeat the procedure 4 times until the sample has been read 5 times all together.

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•When the display shows “STD CON. 2/5”, remove the 20 µg/ml sample, and insert the
40 µg/ml sample into the cuvette holder. Enter 40, using the number pad. Touch “Enter”
and then “OK”.
•Repeat the procedure 4 times until the sample has been read 5 times all together.
•When the display shows “STD CONC. 3/5”, Remove the 40 µg/ml sample, and insert the
60 µg/ml sample into the cuvette holder. Enter 60, using the number pad. Touch “Enter”
and then “OK”.
•Repeat the procedure 4 times until the sample has been read 5 times all together.
•When the display shows “STD CONC. 4/5”, Remove the 60 µg/ml sample, and insert the
80 µg/ml sample into the cuvette holder. Enter 80, using the number pad. Touch “Enter”
and then “OK”.
•Repeat the procedure 4 times until the sample has been read 5 times all together.
•When the display shows “STD CONC. 5/5”, Remove the 80 µg/ml sample, and insert
the100 µg/ml sample into the cuvette holder. Enter 100, using the number pad. Touch
“Enter” and then “OK”.
•Repeat the procedure 4 times until the sample has been read 5 times all together.
•The results from the standard curve are displayed in the protein menu with R2-value and
linear equation. The R2-value should be >0.98.
•Touch the “Unknown”-button, and enter the number 1, using the number pad. Touch
“Enter”.
•Insert the 60 µg/ml reference sample in the cuvette holder and touch “OK”. Repeat 4
times.
•To print the results, touch “Print”. The concentration stated on the printed copy should
be 60 ± 2 µg/ml.
•Touch the “Exit” button to end calibration and linearity test and then touch the
“Setup”-button in the main menu to return to the system setup menu.
C-3-2. UV-Parameters
In UV-parameters you can change the number of times the xenon flash lamp flashes for a single
detector reading. In order to get the best dynamic range for a reading, the number of flashes may
need to be increased (default setting is 1). Increase number of flashes until the maximum digital
count without noise (for a given measurement), is reached. With SpectroArt 200 it is also
possible to adjust the number of times in average, that the detector reads a single measurement.

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Generally, the more times a signal is read, the better signal to noise ratio can be obtained.
However, a high average number means a longer measurement time.
•Touch the “UV Param”-button in the system setup menu.
•Enter a new flash number (1-8), using the number pad. To delete a number, touch the
“Back”-button. Press “Enter”.
•Enter a new average number (1-50), using the number pad. To delete a number, touch
the “Back”-button.
•Press “Enter” to return to the system setup menu.
C-3-3. Touch Pad Settings
In TP-setup, it is possible to restore the touch pad settings if they have been altered. To perform
TP-setup, a password is required.
•Touch “TP Setup” in the system setup menu.
•Enter the password using the number pad. To delete a number, touch the “Back”-button.
•Touch “Enter” and follow the instructions given:
1. Touch the lower left marker.
2. Touch the upper right marker.
3. Touch the marker labelled 1.
4. Touch the marker labelled 2.
5. Touch the marker labelled 3.
6. Touch the marker labelled 4.
•The new calibration data is automatically saved.
C-3-4. Printer Test
Touch the “Printer Test”-button in the system setup menu. SpectroArt 200 automatically prints
out a sinus curve.
C-3-5. Set Default
When touching the “Set Default”-button in the system setup menu, SpectroArt 200 applies the
default settings
C-3-6. Beep Settings
•Touch the “Beep Setting”-button in the system setup menu.

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•To increase the beep-volume, touch the “+”-button next to “Sound Volume” repeatedly,
until the desired volume is reached.
•To decrease the beep-volume, touch the “-“-button next to the “+”-button repeatedly, until
the desired volume is reached.
•Press “Exit” to leave the beep-setting window and return to the system setup menu.
C-3-7. Time/Date Settings
•Touch the “Date/Time”-button in the system setup menu.
•Enter the year (4 digits) using the number pad. To delete a number, touch the
“Back”-button. Touch “Enter”.
•Enter the month (1-12) using the number pad. To delete a number, touch the
“Back”-button. Touch “Enter”.
•Enter the day (1-31) using the number pad. To delete a number, touch the “Back”-button.
Touch “Enter”.
•Enter the hour (1-23) using the number pad. To delete a number, touch the
“Back”-button. Touch “Enter”.
•Enter the minute (0-59) using the number pad. To delete a number, touch the
“Back”-button. Touch “Enter”.
•The updated time and date settings are displayed swiftly. Every time you print your
results, the updated time and date settings will be included in the print-out.
•Press “Exit” to leave the Time/date setting window and return to the system setup menu.
C-3-8. Display Settings
•Touch the “Display”-button in the system setup menu.
•To increase the brightness of the LCD-display, touch the “+”-button next to “Brightness”
repeatedly, until the desired brightness is reached.
•To decrease the brightness of the LCD-display, touch the “-“-button next to the “+”-button
repeatedly, until the desired brightness is reached.
•To increase the contrast of the LCD-display, touch the “+”-button next to “Contrast”,
repeatedly, until the desired brightness is reached.
•To decrease the contrast of the LCD-display, touch the “-“-button next to the “+”-button
repeatedly, until the desired contrast is reached.
•Press “Exit” to leave the display setting window and return to the system setup menu.

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D. Quick Guide to Assays
D-1. DNA/RNA
This function enables measurements of double-stranded DNA, single stranded DNA and RNA.
With the help of a conversion factor, that is, the concentration of nucleic acid at 1.00 absorbance
units at 260 nm, and the dilution factor, the concentration of DNA or RNA in your sample can be
determined. The conversion factor differs according to the type of nucleic acid that is measured.
The default conversion factors used by SpectroArt 200 are 50.0 µg/ml, 37µg/ml or 40 µg/ml for
double-stranded DNA, single-stranded DNA and RNA respectively. Hence, the concentration of
DNA or RNA is calculated as follows;
Conc = A260 nm* conversion factor * dilution factor
Or, if 320 nm correction is enabled;
Conc = (A260 nm –A320 nm) * conversion factor * dilution factor
SpectroArt 200 measures the purity of DNA or RNA in a sample by dividing the DNA or RNA
absorbance values by the absorbance of protein at 280. If 320 nm correction is enabled, the
readings from this wavelength are subtracted from both DNA/RNA absorbance-values and
protein absorbance-values. With SpectroArt 200 you can also measure other forms of nucleic
acids at your customised wavelength with your customised conversion factor.
To measure DNAor RNA, enter the setup menu to choose dsDNA, ssDNAor RNAunder “sample
target”. Choose whether to enable 320 nm-correction or not. Exit setup and read the blank.
When you touch “Sample”, you are asked to enter the dilution factor using the number pad.
Press “Enter” and read the sample.
D-2. Protein
SpectroArt 200 has four different programmed assays for protein concentration measurements.
Three of these (Bradford 595 nm, Lowry 750 nm and BCA 562 nm) are common colorimetric
assays that measure changes in absorption at a fixed wavelength in the visible light region.
Protein can also be measured with SpectroArt 200 within the UV-light range at 280 nm. This
assay can be used when measuring protein in an environment such as a crude cell extract where
nucleic acids are present. It is also possible to choose a custom wavelength anywhere in the
wavelength range for other assays. To choose one of the programmed assays, enter the protein
setup menu. The default setting is Bradford 595 nm. Touch the “method”-button in the setup

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menu until your preferred assay shows. If you want to measure at other wavelength, touch the
“method”-button until “Custom” shows. Touch the button and key in your preferred wavelength
using the number pad. Next, choose the number of points in your standard curve by touching the
“STD Curve”-button and entering a number (2-10), using the number pad. In the same way,
choose number of blank repetitions (1-10), Standard curve point repetitions (1-10) and the
number of times you want to run your unknown samples (1-10). Exit the protein setup menu and
run your blank sample which is the media you have diluted your samples in. After blanking the
spectrophotometer, touch the “STD”-button and enter the unit. Insert your zero-sample and touch
“OK”. When you have measured the absorbance of all of your samples in the standard curve, a
graph with linear equation and R2-value will show on the display. To measure your unknown
samples, touch the “Unknown”-button and enter the dilution factor. When you have measured
the sample, the ABS and calculated concentration will show on the display.
D-3. Kinetics
With the kinetics function you can follow an enzymatic reaction over time, for example the
build-up of a product from a substrate. You specify the total time to measure and then at what
intervals to measure within this period. Enter the setup menu to specify at what wavelength to
measure, e.g. the absorbance of the build-up product. You also specify the total time you want to
measure and the interval time, that is, how often the spectrophotometer reads the sample.
D-4. OD600
The OD600 function as applicable for monitoring. e.g. bacterial growth. Enter the OD600
setup-menu and insert the correct conversion factor for the bacteria to be measured, using the
number pad. Run a blank sample and then touch the “Sample”-button. Insert the dilution factor
using the number pad and then measure the OD600-value of your bacteria. SpectroArt 200
calculates the correct concentration of cells per ml by multiplying the OD600 value by the
conversion factor and the dilution factor.
D-5. Wavelength
When you want to collect absorbance data quick and easy you can choose the wavelength
function. This function allows you to measure the absorbance of up to 9 different wavelengths

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simultaneously. Add or remove wavelengths using the number pad in the setup menu. Then scan
a blank and your sample in the wavelength window.
D-6. Scan
With the scanning function you can analyse an unknown sample within 200 and 800 nm for
either absorbance or transmittance. Enter the wavelength setup menu and choose whether to
measure absorbance or transmittance by touching the “Mode”-button. Key in the maximum and
minimum X-value, that is, the wavelength range you wish to scan. Then touch the
“Y-value”-button to determine the maximum and minimum absorbance values. SpectroArt 200
will generate and display a spectrum with peaks found.
D-7. Functions
D-7-1. DNA/RNA
•Touch the “DNA/RNA”-button in the main menu to launch the DNA/RNA-menu.
•Enter the DNA/RNA-setup menu by touching the “Setup”-button.
•The default setting is dsDNA which has a conversion factor of 50.000 µg/ml. Touch the
“Sample Target”-button once or twice to measure ssDNA or RNA respectively. The
conversion factor changes automatically.
•For customised settings, touch the “Sample Target”-button until the nucleic acid window
displays “custom”. To change conversion factor, touch the “Set Value”-button and then
key in a new value using the number pad. To delete a number, touch the “Back”-button.
When the desired value is displayed, touch the “Enter”-button to return to the
DNA/RNA-setup menu.
•To enable 320 nm background correction, touch the “320 nm correction”-button.
•Touch “Exit” to return to the DNA/RNA menu.
•Before you measure your sample you must blank the spectrophotometer. Touch the
“Blank”-button and insert your blank sample into the cuvette holder. To read the blank,
touch “OK”.
•Remove the blank sample and insert the nucleic acid containing sample.

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•Touch the “Sample”-button and key in dilution factor using the number pad. To delete
number, touch the “Back”-button. Touch “Enter”. If you want the dilution factor to be
applied to all samples, touch “OK”, otherwise, touch “Cancel”.
•To read you nucleic acid containing sample, touch “OK”.
•The results are presented as follows:
Sample ID: XX
ABS@260 nm = XX
ABS@280 nm = XX
Ratio 260/280 nm = XX
Concentration = XX µg/ml
ABS@320 nm = XX
•To analyse more samples, touch the “Sample”-button and key in the new dilution factor
is needed. Insert new sample into the cuvette holder and then touch “OK”. The
ID-number shown will increase by one. To scroll between readings, touch either the
“Prev.” or “Next”-buttons.
•Print results by touching “Print”.
D-7-2. Protein
•Touch the “Protein”-button in the main menu to launch the protein-menu.
•Enter the protein setup menu by touching the “Setup”-button.
•The default setting is Bradford at 595 nm. To change assay, touch the “Method”-button
once for Lowry at 750 nm, twice for BCAat 562 nm, three times for UV-protein at 280 nm
or four times for UV 320 nm.
•For a customised assay, touch the “Method”-“button five times. Then touch the “Change
W. length”-button and key in your desired wavelength (within 200-800 nm) using the
number pad. To delete number, touch the “Back”-button. Touch “Enter” to return to the
protein setup menu.
•To change the number of points in your standard curve, touch the “STD Curve”-button
and key in the new number (2-10) using the number pad. To delete number, touch the
“Back”-button. Touch “Enter” to return to the protein setup menu.
•To change the number of times the zero-sample is read, touch the “Blank Rep.”-button
and key in the number (1-10) using the number pad. To delete number, touch the
“Back”-button. Touch “Enter” to return to the protein setup menu.

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•To change the number of times each point in your standard curve is measured, touch the
“STD Rep.”-button and key in number (1-10) using the number pad. To delete number,
touch the “Back”-button. Touch “Enter” to return to the protein setup menu.
•To change the number of times your unknown samples are read, touch the “Unknown
Rep.”-button and key in new number (1-10) using the number pad. To delete number,
touch the “Back”-button. Touch “Enter” to return to the protein setup menu.
•When your desired settings are shown in the protein setup menu, touch the “Exit”-button
to return to the protein menu.
•Insert a blank sample (the media your protein in diluted in) and then touch “OK”.
•Remove the blank sample and insert your zero protein-sample.
•Touch the “STD”-button and enter unit by touching the “mg/ml”, “µg/ml” or “µg/µl”-button.
Then touch “OK”
•To read the zero-sample, touch “OK” (If you choose to read the zero-sample more than
once, repeat procedure until the STD Conc. Window is displayed).
•Enter the first protein concentration of your standard curve (the lowest concentration)
using the number pad. To delete number, touch the “Back”-button. Touch “Enter”.
•Remove the zero-sample and insert the sample containing the lowest protein
concentration of your standard curve.
•To read the first concentration in the standard curve, touch “OK” (If you choose you read
the sample more than once, repeat the procedure for the designated number of times).
•Repeat the procedure with the second lowest, third lowest concentration etc. of your
standard curve and measure for designated number of times.
•When the standard curve measurements are finished, the curve will be displayed in the
protein menu together with the linear equation and R2-value.
•To print the standard curve results, touch “Print”. All absorbance values for each
standard curve points are printed out as well as linear equation and R2-value.
•Remove the last standard curve point sample and insert your unknown sample into the
cuvette holder.
•Enter the dilution factor using the number pad. To delete number, touch the
“Back”-button. Touch “Enter”,
•To read the unknown sample, touch “OK” (If you choose to read the unknown sample
more than once, repeat procedure the designated number of times)
•The results are presented as follows