Wealtec SpectroArt 200 User manual

SpectroArt 200
Spectrophotometer
Installation and Operation Manual
Version 1.0
Item# 03070
*This instrument is intended for laboratory use only

SpectroArt 200 V1.0
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Index
A. Important Notice ---------------------------------------------------------------- 1
A-1. Warranty
A-2. Technical and Service Contact
A-3. Safety Notice
A-3-1. Certification
A-3-2. Safety Information
B. Introduction ---------------------------------------------------------------------- 3
B-1. Specifications
B-2. Product Description
B-2-1. SpectroArt 200 Spectrophotometer Hardware Overview
C. Installation of SpectroArt 200 Spectrophotometer ------------------ 7
C-1. Package List
C-2. Environment requirement
C-3. SpectroArt 200 spectrophotometer setup
C-3-1. Calibration and Wavelength Linearity Test
C-3-2. UV-Parameters
C-3-3. Touch Pad Settings
C-3-4.PrinterTest
C-3-5. Set Default
C-3-6. Beep Settings
C-3-7. Time/Date Settings
C-3-8. Display Settings
D. Quick Guide to Assays ----------------------------------------------------- 13
D-1. DNA/RNA

SpectroArt 200 V1.0
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D-2. Protein
D-3. Kinetics
D-4. OD600
D-5. Wavelength
D-6. Scan
D-7. Functions
D-7-1. DNA/RNA
D-7-2. Protein
D-7-3. Kinetics
D-7-4. OD600
D-7-5. Wavelength
D-7-6. Scan
D-7-7. Functions
E. Care and Maintenance -------------------------------------------------------- 22
F. Order Information -------------------------------------------------------------- 25

SpectroArt 200 V1.0
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A. Important Notice
Before setting up and operating the instruments of SpectroArt 200 spectrophotometer,
please carefully read these instructions to get familiarized with the installation and operation
process. Instructions should be read by experienced individuals before operating the
instruments.
Any improper usage of the instruments may cause damage. Please refer to the safety
notice included with this equipment.
The instruments shall not be modified or altered in any way. Any modification or alteration
will void the warranty, void the regulatory certifications and create potential safety hazard.
Wealtec is not responsible for any injury or damage caused by using the instruments for any
non-intended purpose or injury as a result of modification of the instruments by any person who
is not authorized by Wealtec Corp.
A-1. Warranty
SpectroArt 200 spectrophotometer is warranted to be free from defects in materials or
workmanship for a period of one year from the original invoice date, under normal usage. Any
defects occurring during warranty period, Wealtec Corp. will repair or replace defective products
or parts without charge unless the defects arise from conditions outlined below. The defects
described below are specially excluded from Wealtec warranty policy.
1. Improper operation of the instrument.
2. Repair or modification by any person who is not authorized by Wealtec Corp.
3. Damage caused by any (in)-direct accident, neglect or misuse.
4. Use fittings or spare parts supplied by anyone other than Wealtec Corp.
5. Damage caused by disaster.
6. Damage caused by any improper solvents or samples
A-2. Technical and Service Contact
Most of the operation details are described in this instruction manual to assist and guide
operator for an appropriate solution. For any other technical/ service questions, please contact
your local representative or contact Wealtec international technical/ service specialist by E-mail:
[e-mail].

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A-3. Safety Notice
A-3-1. Certification
The SpectroArt 200 spectrophotometer is designed to meet the international electrical
safety standards EN61010-1 and EMC regulations. This product meets CE requirements and if
operated according to the guidance of the instruction manual, is certified safe. It is possible that
emissions from this product may interfere with some sensitive appliances when placed nearby or
in the same circuit as those applications. The user should be aware of this potential and take
appropriate measures to avoid interference. Any modification or alteration will void the warranty,
void the regulatory certifications and create potential safety hazards.
A-3-2. Safety Information
Before operating the unit, please note the following precautions:
¾Do not use near flammable materials.
¾Always inspect the SpectroArt 200 spectrophotometer for damaged components
before use.
¾Always connect the system to the correctAC power source
¾Always connect the correct instrument (Computer only) via the serial port
connector.
¾Do not pour liquid into the sample chamber. Thorough clean-up is needed after
each spill (Section E. Care and Maintenance).
¾Do not place object on the SpectroArt 200 spectrophotometer.
¾The SpectroArt 200 spectrophotometer has no customer-serviceable components,
do not open the unit.

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B. Introduction
Spectroscopy is a technique that measures the interaction of molecules in a sample with
electromagnetic radiation. The energy of the light produced by the spectrometer of the
spectrophotometer is used to excite molecules in the sample. For every molecule excited, one
photon of the emitted light is absorbed, hence the transmitted light looses some of its energy.
The absorbance of a solute depends linearly on its concentration and is strongly dependant
upon the wavelength of light. This is why spectrophotometry is performed using monochromatic
light, in which all photons have the same wavelength. A spectrum is obtained when the
absorption of light is measured as a function of its wavelength. The light source of SpectroArt
200 is a xenon flash light. In contrast to traditional light sources such as deuterium or
tungsten-halogen that emit either UV-light or visible light receptively, xenon flash lights emit light
of both UV-and visible light wavelengths.
The concentration of a solute can be calculated by measuring either the light that is
absorbed by the sample or the light that is transmitted through the sample. The absorbance (A)
is related to the intensity of light before (I0) [Incident light] and after (I) [Transmitted light] passage
through the solute;
⎟
⎟
⎠
⎞
⎜
⎜
⎝
⎛
−=
o
ll
A10
log
According to Lambert-Beer’s law, the absorbance depends linearly on the concentration of the
solute, where c is the molar concentration, l is the optical path length in cm and εis the molar
absorption coefficient in L mol-1cm-1;
clA
ε
=
% Transmittance can be calculated as;
100*10100% cl
o
ll
T
ε −
=
⎟
⎟
⎠
⎞
⎜
⎜
⎝
⎛
=
The shows the relationship
between Transmittance and
Concentration andAbsorbance and
Concentration.

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B-1. Specifications
Light source Xenon flash light
Detector Photodiode array
Display Touch screen TFT LCD 5.7”
Wavelength range (nm) 200 – 800
Wavelength accuracy (nm) ± 0.5 from 240 – 640
Absorption/Photometric accuracy (nm) ± 0.001@1AU
Absorption/Photometric repeatability (nm) ± 0.005@1AU
Stray light (nm) 0.1%@220
Photometric noise 0.001@0AU, 550
Spectral bandwidth (nm) < 3
Absorbance range (AU) 0 – 3.0
Data collection speed (msec) 60
Path length (mm) 10
Beam length [Z-axis] (mm) 8.5
Pre-set wavelength 9 Max.
Sample test repetition 10 Max.
System optimization On start up
LCD sleep mode Yes
Standard curve storage 10 Max.
Analytic data storage 99 Max.
Kinetic analysis reading interval range (sec) 2 – 999
Communication port RS232
Number of cuvette 1
Applicable cuvette types Standard/Semi-micro/Micro-volume
Printer Built-in
Dimension [LxWxH] (cm) 39 x 29 x 12
Weight (kg) 5
Input 90 – 264 Vac, 47 – 63 Hz
Operating temperature (˚C)
0 – 40
Humidity : 10% to 90% R.H.
Non-condensing

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B-2. Product Description
B-2-1. SpectroArt 200 Spectrophotometer Hardware Overview
Inte
g
rated thermal
p
rinte
r
Touch screen TFT LCD
Cuvette chamber
Light path axis
Thermal printer paper
Printer paper shaft
Sam
p
le com
p
artment lid
Printer paper compartment lid
Prog./Diagnostic
switch Power input
module
Serial output
port (RS-232)
Fan guard
Power switch
Prog. mode
Diagnostic mode

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Display
Touch screen high resolution TFT display.
Sample compartment lid
Lift the sample compartment lid to insert cuvette into the cuvette chamber.
Integrated thermal printer
Built-in alphanumeric/graphic printer. Press print button to record results immediately.
Printer paper compartment lid
Open the printer paper compartment lid to install new roll of printer paper.
Fan guard
Exhaust air leaves cabinet through this opening.
Universal power input module
Appropriate power cord is connected to the power input module.
Power switch
Instrument main power switch.
Serial output port
RS-232 connection to a computer.
Cuvette chamber
Insert cuvette into the cuvette chamber. Insure that the cuvette is placed in the proper
orientation with respect to the light path (Light path axis = Horizontal – x-axis).

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C. Installation of SpectroArt 200 Spectrophotometer
C-1. Package List
Item Quantity
SpectroArt 200 spectrophotometer
1
Thermal paper roll
1
Power cable
1
Instruction and operation manual
1