Yamato INE800 User manual

Eco Incubator
Model INE800
First edition
●Thank you very much for purchasing
this Yamato eco incubator INE800.
●Please read the “Operating
Instructions” and “Warranty” before
operating this unit to assure proper
operation. After reading these
documents, be sure to store them
securely together with the “Warranty”
at a handy place for future reference.
Warning: Before operating the unit, be sure
to read carefully and fully
understand important warnings in
the operating instructions.
Yamato Scientific Co., Ltd.
This paper has been printed on recycled paper.

Contents
1. Safety precautions....................................................................................................................1
Explanation of pictograms..................................................................................................................... 1
List of symbols ...................................................................................................................................... 2
Warning・Cautions............................................................................................................................... 3
2. Before operating the unit..........................................................................................................4
How to install and preparation before operation................................................................................... 4
Cautions for operation........................................................................................................................... 7
About defrosting of the refrigerator....................................................................................................... 8
3. Names and functions of each part......................................................................................... 10
Main unit.............................................................................................................................................. 10
Structure of the main unit.....................................................................................................................11
Operation panel................................................................................................................................... 12
4. Operating procedures............................................................................................................. 13
Initial setting to clock setting............................................................................................................... 13
Setting a buzzer sound pattern........................................................................................................... 14
Setting the refrigerator mode.............................................................................................................. 15
Setting auto defrost............................................................................................................................. 16
Operating procedures......................................................................................................................... 17
Fixed temperature operation............................................................................................................... 18
Auto stop operation............................................................................................................................. 20
Auto start operation............................................................................................................................. 22
Program operation .............................................................................................................................. 24
How to input a program....................................................................................................................... 26
Manual defrost .................................................................................................................................... 30
Setting the key lock mode................................................................................................................... 30
Setting the recovery mode.................................................................................................................. 31
Setting a calibration offset................................................................................................................... 32
Backup setting..................................................................................................................................... 33
To view indications on the monitor...................................................................................................... 34
Setting the temperature output terminal ............................................................................................. 35
Setting external communication.......................................................................................................... 36
Setting time-up output (optional)......................................................................................................... 57
Setting external alarm output (optional).............................................................................................. 57
About the standalone overheat preventive device.............................................................................. 58
5. Handling precautions .............................................................................................................59
6. Maintenance procedures........................................................................................................60
Daily inspection/maintenance............................................................................................................. 60
7. When the unit is not to be used for a long time or when disposing....................................62
When the unit is not to be used for a long time or when disposing.................................................... 62
Notes about disposition....................................................................................................................... 62
8. When a trouble occurs ...........................................................................................................63
Safety device and error codes............................................................................................................ 63
Troubleshooting................................................................................................................................... 64
9. After sales service and warranty ........................................................................................... 65
When requesting a repair.................................................................................................................... 65
10. Specifications........................................................................................................................ 66
11. Wiring Diagram .....................................................................................................................67
12. List of replacement parts...................................................................................................... 69
13. List of dangerous materials ................................................................................................. 70
14. Standard installation manual ............................................................................................... 71

1
1. Safety precautions
Explanation of pictograms
About pictograms
A variety of pictograms are indicated in this operating instruction and on
products for safe operation. Possible results from improper operation
ignoring them are as follows.
Be sure to fully understand the descriptions below before proceeding to the
text.
Warning
Caution
Indicates a situation which may result in minor injury (Note 2) and
property damages (Note 3.)
（Note 1）Serious injury means a wound, an electrical shock, a bone fracture or intoxication that may
leave after effects or require hospitalization or outpatient visits for a long time.
（Note 2）Minor injury means a wound or an electrical shock that does not require hospitalization or
outpatient visits for a long time.
（Note 3）Property damage means damage to facilities, devices and buildings or other properties.
Meanings of pictograms
This pictogram indicates a matter that encourages the user to adhere to warning
(“caution”included).
Specific description of warning is indicated near this pictogram.
This pictogram indicates prohibitions
Specific prohibition is indicated near this pictogram.
This pictogram indicates matters that the user must perform
Specific instruction is indicated near this pictogram.
Indicates a situation which may result in death or serious injury (Note 1.)

2
1. Safety precautions
List of symbols
Warning
General warnings
Danger!: High
voltage
Danger!: High
temperature
Danger!: Moving
part
Danger!: Hazard
of explosion
Caution
General cautions
Electrical shock!
Burning!
Caution for no
liquid heating!
Caution for water
leak!
For water only
Poisonous
material
Prohibitions
General bans
Fire ban
Do not
disassemble
Do not touch
Compulsions
General
compulsions
Connect ground
wire
Install levelly
Pull outthepower
plug
Regular
inspection

3
1.Safety precautions
Warning・Cautions
Warning
Never operate the unit in an atmosphere containing flammable or explosive gas
Never operate the unit in an atmosphere containing flammable or explosive gas.
Otherwise, an explosion or a fire may result since the unit is not explosion-proof.
See section “13 List of dangerous materials” on page 70
Be sure to connect the ground wire.
Be sure to connect the ground wire correctly. Otherwise, electrical leak may result and cause an
electrical shock or a fire.
Ban on operation when an abnormality occurs
When a smoke or an unusual odor is seen or sensed, immediately turn the power off and pull out
the power plug from the power distribution board. A fire or an electrical shock may result.
Never use electrical power cords bundled.
When these are used bundled, they might overheat causing a fire.
Take care not to damage electrical power cords.
Avoid tightly bend, pull with a strong force or twist to prevent electrical power cords from damaging.
A fire or an electrical shock may result.
Never try to disassemble or alter the unit.
Never try to disassemble or alter the unit.A malfunction, a fire or an electrical shock may result.
Inhibition of octopus wiring.
Octopus wiring using a branching outlet or extensions using a cord reel may cause degradation of
freezing performance or temperature control performance as well as heat generation or a fire from a
voltage drop.
Never place the unit on its side
Never place the unit on its side during transportation or movement to avoid the malfunction of the
refrigerator. If making the unit inclined is inevitable at carrying-in, leave the unit for about a day (for
24 hours) without turning the main power on after carrying-in has been completed.
Caution
When a thunder is heard.
When a thunder is heard, turn the main power off immediately. A malfunction, fire or an electrical
shock may result.

4
2. Before operating the unit
How to install and preparation before operation
Warning
1. Be sure to connect the ground wire.
・Be sure to connect the earth wire (green core wire of the power cord) to the earth wire or
the earth terminal to avoid an electrical shock due to earth leakage.
・Never connect the earth wire to a gas pipe or a water pipe. Otherwise, a fire may result.
・Never connect the earth wire to the earth terminal for a telephone line or to a lightening
conductor. Otherwise, a fire or an electrical shock may result.
・Never use a branching outlet, which might generate heat and cause a danger.
We recommend use of a ground type outlet
tap.
ground type outlet
When there is no ground terminal.
●In this case, class D grounding work is
necessary and please consult your dealer or
our customer service center.
When a bipolar type outlet tap is used
Ground adaptor
●Insert the ground adaptor into a power plug
confirming the polarity of the outlet. Connect the
grounding wire (green) of the ground adaptor to
the ground terminal on the power supply
equipment.
2. Carefully select an installation site. 3.Precautions for defrosting.
●Take special care not to install the unit at a
place described below:
・Where flammable gas or corrosive gas
exists
・Where humidity is high
・Where the ambient temperature is 35•℃
or more
・Where temperature changes severely
・Where subject to direct sunlight
・Where vibration is severe
・Outdoor
Accept water at the drain hose during
defrosting using a drain pan prepared
in advance.
Install this unit at a place with spaces shown
below.
15cm or
more 15cm or
more
Front
15cm or more
1m or more
Note: Drain pan is not included in the product.
Drain pan
Drain hose
To two-pole type
power supply100V
Ground adoptor
Three-pole type power
plug with ground-pole
Ground
wire
W
ｗ
G
Ground-pole
Three-pole type power plug
with ground-pole

5
2. Before operating the unit
How to install and preparation before operation
Warning
3. Never operate the unit in an atmosphere containing flammable or explosive gas.
●Never operate the unit in an atmosphere containing flammable or explosive gas. Since
the unit is not explosion-proof, an arc is discharged when switching the ELB “ON” and
“OFF” and during operation and a fire or an explosion may result.
See the section “13 List of dangerous materials” on page 70 for flammable and explosive gases.
4. Do not attempt to alter the unit
5. Install the unit on a level surface
●The customer shall never attempt to alter
the unit. Otherwise a malfunction may
result.
●Install the unit on a level surface.
Otherwise unexpected troubles or a
malfunction may result.
The unit weight is approx. 135 kg.
When lifting the unit for transportation and installation, carefully handle it by at least two people.
Explosive gas
Flammable gas
Modification

6
2. Before operating the unit
How to install and preparation before operation
6.Power supply
●Use a power supply that meets the electrical capacity of the unit.
Electrical capacity:：AC100V 10A （ELB capacity:15A）
Avoid connecting too many devices using a branching outlet or extending a wire with a cord reel or
refrigerating function and temperature controlling function may degrade due to voltage drop and heat
generation or a fire may occur.
7. Handling of a power cord
●Never use electrical power cords bundled. When these are used bundled, they might
overheat causing a fire.
●Do not convert, forcibly bend, twist or pull the power cord. Otherwise, a fire or an electrical
shock may result.
●Do not place the power cord under a desk or a chair, or sand between objects to avoid it from
being damaged. Otherwise, a fire or an electrical shock may result.
●Do not place the power cord close to a stove or other heat-generating device. Sheath of the
cord may burn and result in a fire or an electrical shock.
●If the power cord should be damaged (exposure of core wire or disconnection), immediately
turn the ELB off, turn the power supply off and ask your dealer to replace the cord. If the unit
is operated with a damaged power cord, a fire or an electrical shock may result.
●Connect the power cord to an appropriate wall outlet.
8. Installation
●The unit might fall down or move by an earthquake or an impact resulting a personal injury.
We recommend making safety measures such as to avoid installing the unit at a place other
than busy places.
9.About fixing the adjusters
There are adjusters at two positions on the
front of the main unit.
When the installation site has been
determoined, follow the procedures below to
fix the adjusters.
①Turn the adjusters until they securely sit
on the floor.
②Check for gaps at four points on the
installation points on the main body.
③Turn the nuts to securely fix the adjusters
under the bottom surface to prevent
loosening.
10.After installation
●The unit may topple down in an earthquake or an unexpected impact and cause personal
injuries. Take appropriate topple-down preventive measures for safety.
①
③

7
2. Before operating the unit.
Cautions for operation
Warning
1.Specimen that must not be used.
This unit is not explosion-proof. Never
attempt to dry or process a specimen that
contains a flammable or explosive
component.
Cautions
2. Put specimens dispersed. 3.Secure space
The withstand load of a shelf board is 15
kg when the load is evenly distributed.
Put specimens dispersed.
1
5
k
g
Too many specimens will prevent correct
temperature control. In order to assure
temperature precision, be sure to use
shelf boards and put specimens
dispersed, and secure at least 30% of
space inside the bath.
4.Take extreme care for specimens below！
Corrosive specimens
Although the unit employs stainless steel plates for its internal surface, take care that they might
corrode with strong acids.Also, take care that vinyl chloride rubber packing may corrode from
acid or alkaline substances, oils or organic solvents.
The evaporator, when a lot of frost is formed on it, may degrade the freezing capacity and the set
temperature may not be maintained. In particular, take care for specimens that contain more
moisture will form more frost on the evaporator. If frosting is found through the frost
observation window, start defrost operation.
You cannot use a device with a larger heat burden that increases temperature in the unit.
Explosive gas
Flammable gas

8
2. Before operating the unit
About defrosting of the refrigerator
When a lot of frost is formed on the evaporator of the refrigerator, the freezing capacity may be
degraded and the set temperature may not be maintained. The unit allows frosting on the
evaporator through the frost observation window at the back of the unit. The rate of frosting will differ
depending on the following conditions.
(1)Operating temperature ･･･ Operating at a lower temperature tends to form more
frost.
(2)External temperature and humidity ･･･ Higher external temperature and humidity tend to form
more frost.
(3)State of specimen in the unit ･･･ More moisture in the specimen tends to form more frost.
The unit allows the following operation modes to prevent frosting, one of which shall be set
according to the operating status. The operation modes can be enabled both during the fixed
temperature and the program operations by pressing the defrost key on the controller operation
panel separately from the fixed temperature operation or the program operation.
1 Manual defrost operation (starting of operation is manual and stopping is auto）
When a lot of frost is formed, start defrost operation. While defrosting operation is started
manually, the operation will automatically stop with the internal timer after about five minutes.
→See page 30 for operating procedures.
2 Automatic defrosting operation (both starting and stopping of operation are automatic）
It is effective for long-term operation of the unit to set the cycle defrost that performs defrosting at
the preset time cycle, or time defrosting that performs defrosting at the specified time every day.
Once you have set the defrosting operation and the time to stop, you can repeat star and stop
defrosting operation automatically at the set intervals. In ordinary cases, you can remove frost by
performing defrosting operation for about five minutes a day. However, select an appropriate
setting based on the actual status of frosting, which will defer depending on the actual operating
conditions.
→See page 16 for operating procedures.
Caution
●Although it depends on the operating conditions, take care for possible influences on
specimen because the temperature in the unit will rise by about 3℃during a five-minute
defrosting.And the temperature on the display may rise by 10℃or more in this case.

9
2. Before operating the unit
About defrosting of the refrigerator
3 Setting the refrigerator operation mode of the refrigerator (continuous & cycle)
The refrigerator operation mode is a function that enables continuous operation, cycle operation
or stopping the refrigerator.
Continuous operation is used when temperature control precision is important. Note that frost
tends to form the more the lower the temperature is at the set temperature range of 0～30℃.
Cycle operation controls by repeating the sequence: operation of the refrigerator (14 minutes)→
pause (six minutes) and has less temperature control precision compared with the continuous
operation but is able to reduce frosting. Note that the refrigerator enters the continuous
operation mode automatically because of the freezing capacity reasons when the set
temperature is 10℃or lower. (When the refrigerator is set at Stop, it will not operate irrespective
of the set temperature.）
Drying of specimen can be reduced by performing the cycle operation. Set the refrigerator to
Stop if its operation is unnecessary. (The refrigerator will automatically set to stop irrespective of
the select operation mode when the set temperature is 40.1℃or more.）
→See page 15 for operating procedures.
Caution
●Even when the cycle operation is set, forming of frost may gradually increase during a long
time of operation depending on the operating conditions. When a lot of frost is formed,
perform defrosting operation or cycle defrosting operation to remove frost. Be sure to put a
drain pan to accept water of melted frost at the drain hose.

10
3. Names and functions of each part
Main unit
Inner
door
Door
Magnet packing
Standalone overheat
preventive device
Controller
Output terminal
RS485 Com. terminal
Temp. output terminal
Other optional terminals
ELB
Φ50mm cable port
With a silicone cap
Serial # and ratings
sticker
Filter
Door lock
Shelf rest/board
Five sets included as
standard
Shelf rest pillar
Holds up to 23
boards
Caster wheel
Power cord
With a plug
Drain hose
Adjuster
Observation window
(for checking frosting on
the evaporator)

11
3. Names and functions of each part
Structure of the main unit
Air inside the unit will be suctioned at the rectifier board at the lower part in the unit with the internal fan
and sent to the air conditioning assembly at the back.
The air conditioning assembly at the back has a refrigerator (evaporator of the refrigerator) and a heater,
and air temperature will be controlled while the air flows through the air conditioning assembly and blow
out at the upper rectifier board and circulates inside the unit.
The temperature controller controls the chiller and the heater in the air conditioning assembly based on
the signals from the temperature sensor installed at the blowing-out part of the internal fan to adjust the
temperature of the air circulating inside the unit.
The refrigerator (chiller) is controlled with an inverter and it implements energy-saving operation by
automatically selecting an optimal cooling capacity according to the set temperature.
※The unit produces operating sounds different from our conventional products because of the
introduction of an inverter. Operating sounds of the refrigerator will also change depending on
the set temperature, which does not indicate any abnormalities.
Internal fan
Observation window
（for checking frosting
on the evaporator）
Wire heater
Evaporator
Temp. sensor
Control: Pt100Ω
Overheat prevention:
K-thermocouple
Shelf boards
Five boards included
Shelf rest
Five sets included
Door
Inner door
reinforcing glass
Shelf rest pillar
Holds up to 23 boards

12
33. Names and functions of each part
Operation panel
No
Name
Description
1
Main display
Displays the internal temperature and error numbers.
2
Sub display
Displays the set temperature and various pieces of information.
3
Setting guide display
Displays guides for various settings.
4
Setting guide display
Displays guides for various settings.
5
REMOTE lamp
Comes on during communication setting.
6
ERROR lamp
Comes on when an error occurs.
7
OPERATE lamp
Comes on during oepration and flashes during operation standby mode.
8
HEATER lamp
Comes on while power is supplied to the heater.
9
REFRIGERATOR lamp
Comes on while power is supplied to the refrigerator and flashes while it is in the
operation standby mode.
10
FIXED TEMP lamp
Comes on during fixed temperature operation.
11
PROGRAM lamp
Comes on during program operation and flashes while an operation mode is
selected.
12
AUTO START lamp
Comes on during auto start and flashes while an operation mode is selected.
13
AUTO STOP lamp
Comes on during auto stop and flashes while an operation mode is selected.
14
MODE key
Used for switching operation modes among ⑩～⑬.
15
POWER key
Used to turning power On/Off by keeping this key pressed longer.
16
DISP key
Displays power consumption, discharged amount of CO2by keeping this key
pressed longer.
17
START/STOP key
Used for starting or stopping the unit.
18
MENU key
Used for setting programs, the buzzer sound, refrigerator modes, the automatic
defrost or the temperature output terminal.
19
Esc key
Used when aborting editing or quitting a menu without entering settings or
setting items.
20
▲(Up) key
Used for changing settings.
21
▼(Down) key
Used for changing settings.
22
/DEF key
Used for moving the cursor to the left during setting or as the manual defrost
key.
23
ENTER key
Used to enter settings or setting items.
24
Standalone overheat
preventive device
Used for setting the standalone overheat preventive device.
⑮
⑭
①
②
③
④
⑦
⑧
⑨
⑤
⑥
⑩
⑪
⑫
⑬
⑰
⑯
⑲
⑱
21
20
23
22
24
▼
Standalone
overheat prevention

13
4. Operating procedures
Initial setting to clock setting
The backup battery contained in the controller is a consumable part. Its rough service life is about
five years but we recommend replacing it with a new one earlier.
※Contact our service department for replacement of the batteries. Make a backup copy
beforehand if a program is being used. See “Backup setting on Page 33.”.
After replacing the batteries, set the clock correctly again to your watch or the time tone.
1
Turning power on
Turn the ELB at the upper part on the right side “ON”.
The Sub display on the controller indicates the current
time.
Keep the controller key pressed longer to turn the
power on.
Main display indicates the internal temperature and the
Sub display indicates the set temperature.
2
Display the screens for setting the
year/month/day and time using the
MENU key
①Press the key.
②Press the key to move the item forward and press
at the item FUNC.
③When the item [bUZZ] appears, press the key to
display the screen for setting the year/month/day.
key; to move the item forward
key; to move the item backward
TIME
TIME

14
4. Operating procedures
Setting the clock
3
Setting the year and month/day
Follow the procedures below to set the date and the clock.
①The TIME lamp flashes and the screen for the year
and month/date appears.
②Press the key.
③Set the western calendar year using the ▲▼ keys
and then press the key.
④Set the month/day using the ▲▼ keys and then
press the key.
※You can move the setting position to the left using
the key.
4
Setting the clock (24-hour format)
①Press the key.
②Press the ENTER key, set the time using the ▲▼
keys and then decide on it using the key.
Enter the time in the 24-hour format.
※You can move the setting position to the left using
the key.
③After completing the setting, press the Esc key twice
to return to the initial screen.
Setting a buzzer sound pattern
1
Setting a buzzer sound pattern
①As in the clock setting, use the and keys to
display Sub display [bUZZ] in the Sub display and then
press the key.
②Select from three buzzer sound patters using the ▲▼
keys and decide on it with the key.
on: All operation sounds will be enabled (initial
setting).
CLK: Click sound only is disabled.
(Sound of the POWER key or other determining
operation is enabled)
oFF: Both click sound and determining operation
sounds are disabled.
※Buzzer sounds when an error occurs even if you
set [bUZZ] to OFF.
③After completing setting, press the Esc key twice to
return to the initial screen.
▼
▼
TIME
TIME

15
4. Operating procedures
Setting the refrigerator mode
1
Setting the refrigerator mode
①Press the key several times until [REFCL]
appears in the Sub display and then press
the key.
②When [REF] appears in the Sub display, press
the key, select a refrigerator mode from three
options using the ▲▼ keys and then decide on it
with the key.
Cnt: Continuous operation (setting: 0～40℃; it is
turned OFF at 40.1℃or higher)
non: The refrigerator will be OFF at all setting
temperatures.
CyC: Cycle operation (setting: 10.1 ℃～40 ℃;
continuous operation will be active at 10℃or
lower; the operation is turned OFF at 40.1℃
or higher）
・Also check the settings below when you have
selected[CyC]:
REF.o: Refrigerator ON time (initial value: 14 min.)
REF.F: Refrigerator OFF time(initial value:6 min.）
※Avoid changing the initial settings because these are
recommended values.
③After completing setting, press the Esc key twice to
return to the initial screen.

16
4. Operating procedures
Setting auto defrost
1
Setting auto defrost
※You cannot change the current auto defrost setting while
the unit is in operation. First, stop operation before setting.
①Press the key several times until [dEFCL] appears in
the Sub display and then press the key.
②When [A-dEF] appears in the Sub display, press
the key and select an auto defrost mode from three
patterns.
non : auto defrost OFF
tIME: cycle defrost (Requiredsettings-dEF:Cy, dEF:ｔM)
CLK : Clock defrost（Required settings-dFS.t、dEF:ｔM）
dFS.t : Defrost time(initial value: 00：00)[TIME]
dEF:Cy: Ordinary run time (initial value:24hrs）[TIME]
Counted at the start of operation
dEF:ｔM: Defrost time(initial value:5min）[MIN]※
Example 1 Cycle defrost
A-dEF :t|ME Every 12 hours after start of operation.
dEF:Cy:12 ⇒Defrosting is performed for five minutes.
dEF:tM:5
Example 2 Clock defrost
A-dEF :CLK Defrosting is performed for five minutes
once every day at 17：00.
dFS:t :17:00 ⇒
dEF:tM:5
※When setting to a larger value, select a value taking
possible increase in internal temperature into
consideration. The margin of increase is about3℃for
five-minute defrosting, which differs depending on the
internal set temperature or other conditions.
③After completing setting, press the Esc key twice to return
to the initial screen.