YSI ProSample Series User manual

ProSample User Manual
PORTABLE AUTOMATIC SAMPLERS
Item# 630123REF
Revision A
For Sales & Service Contact
2650 E. 40th Ave. • Denver, CO 80205
Phone [phone] • Fax [phone]
1-800-833-7958
www.geotechenv.com

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Table of Contents
Safety Information 5
Precautionary Symbols in the Manual 5
Precautionary Symbols on the Instrument 5
Intended Use and General Overview 6
Keypad Overview 7
LED Functionality 8
Package Contents 8
Unpacking 9
Charging the Battery 10
Initial Charge 10
Battery Installation 11
Subsequent Charges 12
No Mains (AC) Power at Sampling Site 12
Mains (AC) Power Available at Sampling Site 12
Installing at a Sampling Site 14
Suction Hose Connection, Positioning, and Length 14
Instrument Setup and Calibration 16
Installing the Peristaltic Pump Tube 16
Turning the ProSample On 17
Pump Calibration 17
Calibration Containers 17
Calibration Procedure 17
Calibration Check 18
After Calibration and/or Calibration Check 18
Installing Sample Bottle(s) 19
Remove the Top of the Housing 19
Prepare Sample Bottles and Install 19
Reattach the Top of the Housing 20
Installing a Distributor Arm 21
Configure Key Instrument Settings 22

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Wiring and Connecting Analog, Digital I/O, and SDI-12 Devices 24
Flow Signal Cable Wiring 24
Calibrating the Analog Signal 25
SDI-12 Cable Wiring 26
SDI-12 with Flying Leads 26
SDI-12 with EXO3 Connector 26
Connection of the Flow Signal Cable and/or SDI-12 Cable 26
Programming 27
Sampling Scenario List and Descriptions 27
Stand-Alone Sampling 28
Analog Input Trigger 32
Analog Input Volume Modulation 38
Digital Pulse Input Trigger 42
Data Pulse Input Event 45
SDI-12 Parameter-Driven Sampling 48
Maintenance and Cleaning 54
Draining the Bottom Sampler Housing 54
Desiccant Replacement 54
Peristaltic Pump Tube Replacement 55
Cleaning 55
Changing the Fuse(s) 56
Long-Term Instrument Storage 56
Factory Reset 57
YSIConnect PC Software 58
Replacement Parts and Accessories 59
Warranty and Liability 66
Technical Support 66
Service Information 66
Appendix A: ProSample Menu Tree 67

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Reference relevant section of this manual for operation and/or safety
information
Appears on the outside of the peristaltic pump cover. This cover
should not be open while the instrument is on, as the user can be
seriously injured if they are exposed to moving parts in the peristaltic
pump assembly.
Safety Information
Please read this entire manual before unpacking, setting up or operating this equipment. Pay attention to all
precautionary statements. Failure to do so could result in serious injury to the operator or damage to the equipment.
Make sure that the protection provided by this equipment is not impaired. Do not use or install this equipment in any
manner other than that specified in this manual.
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NOTICE: The manufacturer is not responsible for any damages due to misapplication or misuse of this
product including, without limitation, direct, incidental and consequential damages, and disclaims
such damages to the full extent permitted under applicable law. The user is solely responsible to
identify critical application risks and install appropriate mechanisms to protect processes during a
possible equipment malfunction.
NOTICE: Indicates a situation which, if not avoided, may cause damage to the instrument
Precautionary Symbols in the Manual
NOTE: Information that requires special emphasis
WARNING: Indicates a potentially hazardous situation which could result in injury
The information contained in this manual is subject to change without notice.
Effort has been made to make the information in this manual complete, accurate, and current.
The manufacturer shall not be held responsible for errors or omissions in this manual.
Consult YSI.com for the most up-to-date version of this manual.
When viewing this document as an AdobeTM PDF, hovering
your cursor over certain phrases will bring up the finger-point
icon. Clicking elements of the Table of Contents, website URLs,
or references to certain sections will take you automatically
to those locations.
THIS IS AN INTERACTIVE DOCUMENT
Precautionary Symbols on the Instrument

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Thank you for purchasing a YSI portable automatic sampler. Samplers in the ProSample series are designed to collect
field samples and to provide temporary storage before they are analyzed in a laboratory.
The ProSample portable automatic samplers are designed for use in surface water, stormwater, and wastewater
applications. These samplers can be used in a variety of scenarios, most of which are described in this manual. If
you have a question regarding the use of a sampler in your project, please contact YSI (YSI.com/contact-us) or an
authorized YSI distributor.
There are four samplers in the ProSample series. Content in this user manual applies to all ProSample models unless
otherwise noted. ProSample models include:
• ProSample P: full-size portable sampler
• ProSample P-12: full-size portable sampler with SDI-12 connectivity
• ProSample PM: mini portable sampler
• ProSample PM-12: mini portable sampler with SDI-12 connectivity
Composite sampling can be completed with all ProSample models. In this type of sampling, a single container is used
to collect multiple samples, resulting in a mixture of all samples collected.
Discrete sampling can be completed with the ProSample P and ProSample P-12. In this type of sampling, samples are
placed in different bottles. There are several bottle configurations available for the purpose of discrete sampling.
All ProSample models have the following features:
• Robust PE, double-walled, insulated housing for temperature control.
• Easy tube replacement for minimal downtime.
• Spring-loaded roller bearings in the peristaltic pump providing long tubing life.
• Highly accurate sample volume using two capacitive sensors in the peristaltic pump for volume control.
• Simple programming and calibration.
• Long battery life.
• Non-volatile data memory for up to 5 years, so you never have to worry about losing your data if power is lost.
Intended Use and General Overview
Intended Use and General Overview

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Keypad Overview
ProSample Keypad
Select the desired menu.
Confirmation of entered values and selections. A confirmed
selection is indicated with .
Enter Key
Move within the menu.
Move to the next menu.
Entry/change of values.
Arrow Keys
Selection within the menu.
Scrolling within the data memory or bottle memory.
Entry/change of values.
Arrow Keys
Return to higher menu level.
The activity is canceled.
Back Key
Terminate sleep mode.
Back Key
Press for at least 5
sec
Intended Use and General Overview

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LED Functionality
There is an LED on the front of each ProSample that functions as follows:
• OFF: Device power is off, battery is dead, or no program is running
• Solid Green: Program is running and there are no errors
• Blinking Green: Program is running, but the battery is getting low
• Solid Red: An error has occurred. The display should be viewed for more information regarding the cause
of the error
• Blinking Red: Battery capacity is critically low
Included with each ProSample:
• *5 meter suction hose with screw connection and sinker weight
• 10L PE container (only the ProSample PM and the ProSample PM-12)
• Battery
• USB cable
• Two peristaltic pump tubes
• Printed copy of the ProSample Getting Started Guide
*Up to a 30 meter suction hose can be used with any ProSample model.
Please note the following items are not included with any ProSample. These items must be purchased separately.
• Bottle sets and distributor arms for the ProSample P and ProSample P-12
• SDI-12 cable for the ProSample P-12 and ProSample PM-12
• Flow signal cable
• Battery charger
• Other accessories such as strainers
Please see the Replacement Parts and Accessories section on page 60 for more details and item numbers, including
longer suction hose lengths.
The ProSample User Manual, installation file for YSIConnect, and the ProSample Ordering Guide can be found at
YSI.com/ProSample.
Package Contents
Intended Use and General Overview / Package Contents

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Carefully unpack the unit and accessories, and inspect for shipping damages. Compare received parts with materials
listed in the Package Contents section. Notify YSI immediately of any damage or missing parts. Save all packing
materials until satisfactory operation is confirmed.
Unpacking
Removing the top tray (1) and the ProSample (2) from the packaging
Unpacking

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Charging the Battery
Initial Charge
The battery must be completely charged prior to first use. This should take approximately 14 to 16 hours.
NOTICE: A total discharge of the battery can result in damage to the battery. A protective mechanism built-in
which automatically switches off the device when the voltage is too low (9V or less). However, when
storing the battery long-term, regularly connect the battery to the charger to top off the charge, as this
will ensure the battery does not completely discharge.
NOTE: Please include the battery if the ProSample is returned to YSI for service — see the Technical Support
section on page 66 and the Service Information section on page 66.
To charge, connect a ProSample battery charger to the battery. Once the red light on the power supply turns to green,
the battery is fully charged and can be installed in the ProSample unit.
NOTE: There are three different battery chargers available for the ProSample. Please see the Replacement Parts
and Accessories section on page 60 for a list of available chargers.
Connection of the ProSample battery to the battery charger
Charging the Battery

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Battery Installation
The battery pack is not installed in the ProSample during shipment.
NOTICE: It is recommended to disconnect the battery from the instrument when transporting the ProSample.
To install the battery.
1. Open the ProSample cover.
2. Insert the battery pack into the battery tray.
3. Secure it with the strap.
4. Connect the battery plug to the battery.
Battery installation
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Charging the Battery

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Subsequent Charges
NOTE: When charging a new battery, it is recommended to use the steps in the Initial Charge section
on page 10.
The battery must always be installed in the ProSample when the sampler is in use, as the battery is the only way to
power the sampler. The ProSample will shut off when the battery drops below 9V.
There are two options for charging the battery that depend on access to mains (AC) power.
No Mains (AC) Power at Sampling Site
The battery can be periodically charged by removing it from the instrument and charging it with a ProSample battery
charger whenever a recharge is needed (see the Initial Charge section). This is most the common method of powering
the instrument when there is no mains (AC) power source located near the ProSample unit.
Mains (AC) Power Available at Sampling Site
It is also possible to keep the battery continuously connected to mains (AC) power. In this scenario, the battery is kept
installed in the instrument and an optional Y-cable (item # 0069810) is used to simultaneously connect the battery to
the battery charger and the instrument. This is the most common method of powering the instrument where there is
a mains (AC) power source located near the ProSample unit. Since the battery charger will typically be outdoors and/
or exposed to water, an IP-67 battery charger is recommended (see the Replacement Parts and Accessories section on
page 60).
NOTE: The battery must be completely charged prior to first use. It is recommended to initially charge the battery
by connecting the battery directly to the battery charger (i.e. the ‘Y’ cable is not used) – see the Initial
Charge section on page 10. The Y-cable can be used once the battery is initially charged.
For continuous connection of the battery to AC power via the Y-cable:
1. Open the instrument cover.
2. Disconnect the battery from the instrument.
3. Connect the Y-cable to the instrument.
4. Connect the Y-cable to the battery.
5. Connect the Y-cable to the battery charger.
Charging the Battery

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Using the Y-cable to keep the battery continuously connected to AC power
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Charging the Battery

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The ProSample unit must be placed on a steady, level surface.
YSI recommends carefully planning out the installation location before drilling any holes, cutting any tubing, etc.
The ProSample should be installed in a location where the sample temperature is between 0 °C and 40 °C. The
sampler should not be exposed to explosive substances or an environment with an explosive atmosphere.
WARNING: If the unit is to be continuously connected to AC power, ensure the electrical supply is sufficiently
protected against short circuits.
Suction Hose Connection, Positioning, and Length
The suction hose is the hose that will transport water from the sampling site to the sampler. The other key consideration
when installing a ProSample unit is the correct positioning of the suction hose. One end of the suction hose connects
to the intermediate tube, while the other end of the suction hose is placed in the environment where samples are
collected.
Installing at a Sampling Site
Suction hose connection to the intermediate tube
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NOTICE: The connectors on the ProSample intermediate tube and suction hose are ¾ inch-14BSPP. If not using
a YSI suction hose (5, 10, and 20 meter available), ensure the suction hose being used has a ¾ inch-
14BSPP connection. A ¾ inch-14BSPP female connection (item # 630127) is available if the suction
hose being used does not have a ¾ inch-14BSPP connection.
There is a small gasket zip-tied to the suction hose upon delivery. Place this gasket in the suction hose connector
before connecting the suction hose to the intermediate tube. This gasket prevents air from getting in the line, thus
ensuring accurate dispensing of solution.
INTERMEDIATE TUBE
SUCTION HOSE
SUCTION HOSE CONNECTION
TO INTERMEDIATE TUBE
Installing at a Sampling Site

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Positioning of the ProSample unit and suction hose
The suction hose cannot be longer than 30 meters. In addition, it should be positioned so it is descending in
elevation from the sampler to the sampling point. Locations marked with a red ‘X’ on the figure below are examples
where this requirement is not satisfied. Placing the hose in a PVC pipe can help ensure this requirement is met.
The hose should not be coiled, as this can allow water to collect in the excess hose and impede the travel of water
through the hose. If the suction hose is longer than needed, remove the sinker weight at the end of the suction hose,
cut the suction hose to the desired length, then reinstall the sinker weight.
Installing at a Sampling Site

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Installing the Peristaltic Pump Tube
The peristaltic pump tube does not come pre-installed in the ProSample. There are two peristaltic tubes included with
each ProSample — one can be found under the lid of the ProSample, while the other is in the top tray of the ProSample
packaging. To install the peristaltic pump tube:
1. Turn the ProSample OFF and disconnect the battery.
WARNING: Do NOT attempt to install a peristaltic pump tube or service the pump while the ProSample is ON.
The moving components of the pump can cause serious injury.
2. Use a flathead screw driver to loosen the bolt on the outside of the peristaltic pump cover.
3. There are two tube connectors the peristaltic pump tube will connect to — one connector is on the intermediate
tube while the other is on the composite sampling tube. Slide the connectors out of the side of the pump
housing.
4. Connect the peristaltic pump tube to each tube connector and slide the connectors back into the side of the
pump housing.
5. Place the peristaltic pump tube on the outside of the rollers of the peristaltic pump. You may need to turn the
roller assembly in order to get the pump tube to properly seat in the pump housing. Refer to the image below.
6. Close the battery cover to the peristaltic pump and tighten the bolt using a flathead screwdriver. Reconnect the
battery.
Instrument Setup and Calibration
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Installed pump tube
Instrument Setup and Calibration

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Turning the ProSample On
Press the ON/OFF button once to turn the ProSample on. Press and
release the button again to turn the unit off.
Pump Calibration
The peristaltic pump must be calibrated at each sampling site, as the length and gradient of the suction hose impact
the calibration result.
NOTE: Calibration in a lab setting can be completed to verify the instrument is functioning properly, but it is still
highly recommended to recalibrate the instrument once at the sampling site.
Calibration Containers
The ProSample is calibrated to an upper and lower volume, resulting in a 2-point calibration. The upper calibration
will dispense a large volume of solution, typically over 1000 mL, whereas the lower calibration will dispense a small
volume, around 250 mL.
The volume dispensed during calibration will need to be accurately measured. It is recommended to obtain a large,
plastic graduated cylinder and a small, plastic graduated cylinder when measuring the amount of solution dispensed
during calibration.
Calibration Procedure
The following steps describe how to calibrate the ProSample peristaltic pump:
Turning on the ProSample
Calibration with large graduated cylinder
1. Turn the ProSample OFF.
2. Remove the top of the ProSample housing and flip it over.
3. Remove the plastic ‘elbow’ piece from the end of the composite
sampling tube. There is a plastic bracket that keeps the
composite sampling tube in place during sampling – remove
the tube from this bracket.
4. Reinstall the top of the ProSample housing.
5. Pull the composite sampling tube out of the ProSample
housing. This will allow solution to be poured directly into a
graduated cylinder.
6. Place a graduated cylinder with a volume of at least 2000 mL
under the composite sampling tube.
7. Turn the ProSample ON.
8. From the Main Menu, navigate to Setup g System Settings
g Calibration Vol g Volume Calibration g Start Upper
Volume. After pressing the Enter key, the ProSample will purge
the line and then dispense a large amount of solution into the
graduated cylinder three separate times.
Instrument Setup and Calibration

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Calibration Check
The ProSample can dispense a user-specified amount of solution, allowing the pump calibration to be verified. To
complete a calibration check:
After Calibration and/or Calibration Check
1. Turn OFF the ProSample.
2. Insert the composite sampling tube into the hole in the
ProSample housing.
3. Remove the top of the ProSample housing and flip it over.
4. Reinstall the plastic ‘elbow’ piece on the end of the composite
sampling tube. There is a plastic bracket that keeps the
composite sampling tube in place during sampling — place the
tube in this bracket.
5. Reinstall the top of the ProSample housing.
1. From the Main Menu, navigate to Manual Sample g In Present
Bottle.
2. Place a suitable graduated cylinder underneath the sampling
tube.
3. Enter the amount of Sample Volume to be dispensed. It is
recommended to enter the amount of solution that will typically
be dispensed when sampling. Press Enter.
4. Observe the amount of solution dispensed and recalibrate if
needed.
9. Do NOT remove the graduate cylinder from underneath the
sampling tube until Enter Actual Value is displayed on the
screen. Enter the amount of solution that was dispensed and
press the Enter key.
10. Start Lower Volume should appear on the screen. Place a
graduated cylinder with a volume of at least 250 mL under
the composite sampling tube. After pressing the Enter key,
the ProSample will purge the line and then dispense a small
amount of solution into the graduated cylinder three separate
times.
11. Do NOT remove the graduated cylinder from underneath the
sampling tube until Enter Actual Value is displayed on the
screen. Enter the amount of solution that was dispensed and
press the Enter key.
Instrument Setup and Calibration

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Installing Sample Bottle(s)
There are several bottle configurations available for the ProSample P and ProSample P-12. Plastic (PE) and glass bottle
sets are available. Please see the Replacement Parts and Accessories section on page 60 for a complete listing of
available bottle sets.
If multiple bottles will be installed in a ProSample P or ProSample P-12, a distributor arm must be installed. Please see
Installing a Distributor Arm on page 21.
A single 10 liter PE bottle can be placed in the ProSample PM and ProSample PM-12, but no other bottles can be used,
as a distributor arm cannot be installed in the mini samplers.
Remove the Top of the Housing
Disconnect the clips that hold the top part of the housing to the rest of the instrument. Lift the top part of the housing
off the instrument.
Prepare Sample Bottles and Install
Ensure the sample bottles are clean and suitable for use before installing in the bottom ProSample housing.
NOTE: A fixing plate is included with some bottle sets. If a fixing plate is included with your bottle set, it should
also be installed in the bottom ProSample housing.
Removal of the top part of the ProSample housing
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Instrument Setup and Calibration

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Reattach the Top of the Housing
After installing the sample bottle(s), reattach the top part of the housing by lining it up with the bottom part of the
housing. Use the clips on the side of the instrument to secure the top part of the housing to the bottom.
Installation of plastic (PE) bottles
Installation of glass bottles
Bottle #1 position and filling direction on the ProSample P and ProSample P-12 housing
Instrument Setup and Calibration