Zeiss Colibri 7 User manual

Colibri 7
Operating Manual

ZEISS Copyright / Trademark Colibri 7
2 423052-7344-001 02/2017
Knowledge of this manual is required for operation of the device. You should therefore familiarize
yourself with its contents, paying particular attention to instructions concerning safe handling of the
device.
We reserve the right to make changes to the product in the light of technical developments. The manual
is not covered by an update service.
© Unless expressly authorized, dissemination and duplication of this document and commercial
exploitation or communication of its contents are not permitted. Persons in contravention of this
copyright shall be liable for damages.
All rights reserved in the event of granting of patents or registration as a utility model.
All names of companies and products mentioned in this operating manual may be trademarks or
registered trademarks. Third party products are cited for information purposes only. This does not
represent approval or recommendation of these products.
Carl Zeiss Microscopy GmbH accepts no liability for the performance or use of these products.
Editor: Carl Zeiss Microscopy GmbH
Carl-Zeiss-Promenade 10
07745 Jena, Germany
microscop[email protected]m
www.zeiss.com/microscopy
Carl Zeiss Microscopy GmbH
Königsallee 9-21
37081 Göttingen, Germany
Operating manual number: 423052-7344-001
Date of publication: Version 5 – 20.02.2017
authorized dealer:
Pulch + Lorenz microscopy
Am Untergrün 23, -79232 March
tel: [phone]
fax: [phone]
mail: [email protected]
web: pulchlorenz.de

Colibri 7 Contents ZEISS
02/2017 423052-7344-001 3
CONTENTS
Page
1INTRODUCTION................................................................................................................ 5
1.1 Notes on device safety........................................................................................................ 5
1.2 Warning labels ................................................................................................................... 8
1.3 Warranty notes................................................................................................................... 9
2DESCRIPTION OF THE DEVICE........................................................................................ 10
2.1 Name, intended use, and main features ............................................................................ 10
2.2 Components overview...................................................................................................... 11
2.3 Technical data .................................................................................................................. 12
2.4 Operating and function controls ....................................................................................... 13
2.4.1 Connection panel on the lamp module ............................................................................. 13
2.4.2 LED Trigger Box for Colibri 7 (optional).............................................................................. 14
2.4.3 Control panel for Colibri 7 (optional)................................................................................. 15
3START-UP ....................................................................................................................... 17
3.1 Terminal diagrams ............................................................................................................ 17
3.1.1 Terminal diagram for stand-alone operation ...................................................................... 17
3.1.2 Terminal diagram with PC and motorized microscope........................................................ 18
3.2 Unpacking Colibri 7.......................................................................................................... 19
3.3 Attaching Colibri 7 to the microscope and connecting it .................................................... 19
3.4 Configuring Colibri 7 with MicroToolBox (MTB)................................................................. 21
4OPERATION .................................................................................................................... 23
4.1 Starting operation of Colibri 7 for the first time ................................................................. 23
4.2 Controlling the Colibri 7 solid-state light source................................................................. 23
4.2.1 Control via TFT display of the microscope.......................................................................... 24
4.2.2 Control via the control panel for Colibri 7.......................................................................... 25
4.2.3 Operation with PC via the ZEN 2.3 (blue edition) software ................................................. 27
5CARE AND SERVICE........................................................................................................ 32
5.1 Care................................................................................................................................. 32
5.2 Service ............................................................................................................................. 33
6ANNEX............................................................................................................................ 34
6.1 Property rights.................................................................................................................. 34

ZEISS List of illustrations Colibri 7
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LIST OF ILLUSTRATIONS
Fig. 1 Warning labels on the lamp module ....................................................................................8
Fig. 2 Warning label on reflected light mount of microscope..........................................................9
Fig. 3 Components overview of the Colibri 7 solid-state light source ............................................11
Fig. 4 Connection panel on the lamp module ..............................................................................13
Fig. 5 LED Trigger Box for Colibri 7 (front and rear side)...............................................................14
Fig. 6 Control panel for Colibri 7 – operating and function controls .............................................15
Fig. 7 Terminal diagram for stand-alone operation.......................................................................17
Fig. 8 Terminal diagram with PC and motorized microscope for fast acquisition............................18
Fig. 9 Attaching the lamp module to the microscope...................................................................19
Fig. 10 Connecting the lamp module and components ..................................................................20
Fig. 11 MTB – Configuring the connection type.............................................................................21
Fig. 12 MTB – Configuring the lamp module type..........................................................................22
Fig. 13 TFT display, page "Colibri LEDs": one LED switched on and activated for brightness
adjustment .......................................................................................................................24
Fig. 14 TFT display, page "Colibri LEDs": three LEDs switched on and activated for brightness
adjustment .......................................................................................................................24
Fig. 15 Control panel for Colibri 7.................................................................................................25
Fig. 16 Colibri 7 control via "Microscope Components" tool..........................................................27
Fig. 17 Control menu for Colibri 7 ................................................................................................27
Fig. 18 "Locate" tab – "Camera" tool ..........................................................................................28
Fig. 19 Colibri 7 control via "Channels" tool .................................................................................29
Fig. 20 "Acquisition" tab – "Acquisition mode" tool .....................................................................30
Fig. 21 Colibri 7 control via "Imaging Setup" tool .........................................................................31

INTRODUCTION
Colibri 7 Notes on device safety ZEISS
02/2017 423052-7344-001 5
1INTRODUCTION
1.1 Notes on device safety
The Colibri 7 solid-sate light source has been designed, produced and tested in compliance with the
European standard DIN EN 61010-1 (IEC 61010-1), "Safety requirements for electrical equipment for
measurement, control and laboratory use", meets the requirements set forth in the RoHS Directive
2011/65/EC and carries the mark.
The Colibri 7 solid-state light source meets the Low Voltage Directive 2014/35/EU and the EMC Directive
2014/30/EC.
The devices must be disposed of in compliance with the WEEE Directive 2012/19/EC.
This operating manual contains information and warnings that must be followed by the operator.
The following warning and information symbols are used in this operating manual:
CAUTION
This symbol indicates a potential hazard to the user.
CAUTION LED radiation!
LED risk group 3 according to DIN EN 62471:2009 when the user looks directly into the
Colibri 7 light output.
UV radiation is emitted.
Any direct looking into the Colibri 7 light output is to be avoided.
Failure to heed this caution may result in eye damage.
Skin and eye irradiation is to be minimized by appropriate shielding.
WARNING of possible laser radiation!
The safety screw for fastening the lamp module to the microscope may on
ly be removed by
ZEISS Service (not a customer interface). Otherwise there is a risk of eye damage upon laser
coupling.
CAUTION: High-energy UV radiation!
Risk of damage to the eyes and skin!
CAUTION
Disconnect the device from the line before any intervention!
CAUTION
Hot surfaces on lamp module!
ATTENTION
This symbol indicates a potential hazard to the instrument or system.
NOTE
This symbol designates information that should be closely followed.

INTRODUCTION
ZEISS Notes on device safety Colibri 7
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Depending on the design of the microscope, the risk for the user must be assessed as being markedly
lower.
When it comes to the intended use of the Axio Observer microscope with Colibri 7, for example, the risk
status of the overall system is downgraded to LED risk group 2 according to DIN EN 62471:2009.
Colibri 7 and its original accessories may only be used for the purposes described in this operating
manual.
Particular attention should be paid to the following:
Use Colibri 7 for ZEISS microscopes only. The manufacturer cannot assume any liability for any
other applications of the instrument, including the use of individual modules or components.
This also applies to any service or repair work that is not carried out by authorized service
personnel. Failure to comply with this shall render all warranty claims invalid.
To ensure safe operation of the Colibri 7 solid-
state light source in connection with the
microscope, pay attention to the operating manual of the microscope.
Colibri 7 is not to be used in explosive environments.
Keep combustible and easily inflammable materials away from the light beam and its
surroundings.
Colibri
7 is not equipped with any special devices for protection from substances that are
corrosive, potentially infectious, toxic, and
radioactive or other substances that may be
hazardous to health. Observe all legal regulations, particularly the relevant national accident
prevention regulations when handling such substances.
Disconnect the power plug of the desktop power supply unit prior to assembly / disassembly of
the lamp module.
Only specially authorized experts or service staff are permitted to open the lamp module.

INTRODUCTION
Colibri 7 Notes on device safety ZEISS
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The desktop power supply unit of the Colibri 7 solid-state light source permits mains voltages in
the 100 V to 240 V ±10 %, 50 Hz
to 60 Hz range without the voltage setting on the
instrument having to be changed.
The desktop power supply unit should not be brought into contact with moisture. If the
housing is damaged, the desktop power supply unit should be taken out of use. Colibri 7 must
only be operated with the desktop power supply unit contained in the scope of delivery.
Before connecting the desktop power supply unit to a power outlet check whether it is suitable
for the available line voltage.
The power plug of the desktop power supply unit must be inserted in an outlet featuring a
grounding (earth) contact. The protective capacity must not be rendered ineffective by the use
of extension cables with no grounding conductor.
Do not substitute
detachable power cables with power cables that do not meet the
specifications. Only the specified power cables should be used.
If it is determined that protective measures are no longer effective, the instrument must be
taken out of service and secured
against inadvertent operation. To repair the instrument,
contact the ZEISS service team in Germany (see page 33
) or your local ZEISS overseas
representative.
Colibri 7, if equipped with the corresponding LED modules, emits ultraviolet radiation that may
cause burns to the eyes and skin. Never look directly into the light and avoid direct skin
exposure. When operating the microscope, always use the protective
devices belonging to the
instrument.
Never look into the light beam –
neither with nor without optical instruments, even not if you
simply want to observe the specimen. Failure to follow these instructions may result in eye
injury!
Take appropriate protective measures if the specimen releases noxious gases, dust, and vapors,
secondary radiation or explosive substances as a result of the UV radiation!
Hot surfaces on lamp module! Avoid touching the hot lamp housing.

INTRODUCTION
ZEISS Warning labels Colibri 7
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Placing objects against or covering ventilation slats of the control unit may lead to heat build-up
that may damage the device and, in extreme cases, cause a fire. Always keep the ventilation
slots clear and ensure that no objects enter or fall into the instrument through the ventilation
slats.
Dust and dirt may impair the instrument’s performance. Therefore, the device must largely be
protected from these influences and covered with the dust cover when not being in use.
Always check whether the instrument is switched off before you cover it.
Defective devices must not be disposed of with the household waste. Dispose them of in
compliance with the relevant legal requirements or return them to the manufacturer of the
device.
The manufacturer of the device is under the legal obligation to take defective devices back.
1.2 Warning labels
Standard labeling
Fig. 1 Warning labels on the lamp module

INTRODUCTION
Colibri 7 Warranty notes ZEISS
02/2017 423052-7344-001 9
Labels on the reflected light mount of the microscope for laser application with Colibri 7
Fig. 2 Warning label on reflected light mount of microscope
The safety screw (Fig. 2/1) on the reflected light mount for
fastening the lamp module to the
microscope may only be removed by ZEISS Service (not a customer interface). Otherwise there is
a risk of eye damage upon laser coupling.
1.3 Warranty notes
The manufacturer guarantees that the device is free of material or manufacturing defects upon delivery.
Any defects must be reported immediately and steps taken to minimize damage. If such a defect is
reported, the device manufacturer shall be obliged to correct the default, either by repairing the device or
replacing it with a new one, at the manufacturer’s discretion. No warranty is given for defects caused by
natural wear (particularly of wearing parts) and improper use of the device.
The device manufacturer shall not be liable for damage caused by misuse, negligence or any other
tampering with the device, particularly the removal or replacement of instrument components, or the use
of accessories from other manufacturers. Such actions shall invalidate any warranty claims.
With the exception of the work described in this manual, no maintenance or repair work is to be carried
out on these microscopes. Repairs may only be carried out by ZEISS Service or individuals specially
authorized by ZEISS Service. In the event of a problem occurring with the instrument, please contact the
ZEISS Service in Germany (see page 33) or your local ZEISS overseas representative.

DESCRIPTION OF THE DEVICE
ZEISS Name, intended use, and main features Colibri 7
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2DESCRIPTION OF THE DEVICE
2.1 Name, intended use, and main features
Manufacturer’s designation: Colibri 7
Colibri 7 is designed to be used as a light source for reflected light fluorescence applications.
It can be used in connection with the following upright microscopes:
−Axio Imager
−Axio Examiner
−Axioplan 2
−Axioskop 2
−Axioskop 2 FS
−Axioskop 40
−Axio Scope.A1
and with the following inverted microscope:
−Axio Observer
−Axio Vert.A1
−Axiovert 200
The device has the following essential features:
−High-end LED light source for fluorescence applications with visible and UV light for direct
connection to the microscope, no adjustment necessary
−Four different variations (fixed LEDs), equipped with four to seven excitation lines with different
emission wavelengths and fixed excitation filters
−LED emission is individually adjustable for each LED in increments of 1 % (1 – 100 %)
−Standby mode to reduce power consumption
−Real-time stabilization of brightness for low-noise image captures
−Long-time stabilization and performance optimization to improve the comparability of image
captures
−Integrated and motorized switchable excitation filters
−Calibrated and monitored lamp intensity to improve quantification of image results
−Very quick switching on, over and off of the LED modules, no shutter required
−Control via the TFT display of Axio Observer 7 / Z1 stands via CAN connector
−Optional control via software ZEN 2.3 (blue edition), manual control panel or Trigger Box
−Integrated interface for triggered image capture from ZEN 2.3 (blue edition)
−Specific filter sets (as P&C filter modules without excitation filters) for the LED variations deliverable
−Minimum heat development
−Guaranteed lifetime 15,000 h for each bulb per LED line
−Easy integration in 3rd party software packages via LED Trigger Box

DESCRIPTION OF THE DEVICE
Colibri 7 Components overview ZEISS
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2.2 Components overview
Fig. 3 Components overview of the Colibri 7 solid-state light source

DESCRIPTION OF THE DEVICE
ZEISS Technical data Colibri 7
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2.3 Technical data
Dimensions
Lamp module (length ×width ×height) ..................................................... 167 mm x 183 mm x 103 mm
LED Trigger Box (length ×width ×height) .................................................. 220 mm x 245 mm x 105 mm
Control panel (length ×width ×height) ....................................................... 180 mm x 110 mm x 70 mm
Weight
Lamp module ....................................................................................................................up to 1,900 g
LED Trigger Box ..............................................................................................................approx. 2,100 g
Control panel.................................................................................................................... approx. 590 g
Excitation wavelengths
Solid-state light source Colibri 7, type RGB-UV ..................................................... 630, 555, 475, 385 nm
Solid-state light source Colibri 7, type RYB-UV ..................................................... 630, 590, 475, 385 nm
Solid-state light source Colibri 7, type R[G/Y]B-UV .........................................630, 590, 555, 475, 385 nm
Solid-state light source Colibri 7, type R[G/Y]CBV-UV ....................630, 590, 555, 511, 475, 430, 385 nm
Ambient conditions
Transport (in packaging):
Permissible ambient temperature...............................................................................-40 °C to +70 °C
Storage:
Permissible ambient temperature..............................................................................+10 °C to +40 °C
Permissible relative air humidity (no condensation)............................................... max. 75 % at 35 °C
Operation:
Permissible ambient temperature..............................................................................+10 °C to +40 °C
Permissible relative air humidity (no condensation)............................................... max. 75 % at 35 °C
Altitude of operating site ................................................................................................max. 2000 m
Atmospheric pressure .........................................................................................800 hPa to 1060 hPa
Degree of pollution...........................................................................................................................2

DESCRIPTION OF THE DEVICE
Colibri 7 Operating and function controls ZEISS
02/2017 423052-7344-001 13
Operating data
Operating area ...............................................................................................................Enclosed rooms
Protection class ......................................................................................................................................I
Ingress protection rating ..................................................................................................................IP 20
Electrical safety ...........................................................in compliance with DIN EN 61010-1 (IEC 61010-1)
............................................................................................. and conforming to CSA and UL regulations
Overvoltage category.............................................................................................................................II
Radio interference suppression........................................................ in compliance with EN 55011 Class B
Noise immunity ............................................................................... in accordance with DIN EN 61326-1
Line voltage of controller module ..................................................................... 100 to 240 VAC (±10 %)
Line frequency...................................................................................................................... 50 to 60 Hz
Power consumption of controller module .......................................................................................70 VA
Colibri 7 overall LED classification ...........................LED risk group 3 according to DIN EN 62471:2009
Solid-state light source Colibri 7, type RGB-UV .............LED risk group 3 according to DIN EN 62471:2009
Solid-state light source Colibri 7, type RYB-UV..............LED risk group 3 according to DIN EN 62471:2009
Solid-state light source Colibri 7, type R[G/Y]B-UV ........LED risk group 3 according to DIN EN 62471:2009
Solid-state light source Colibri 7, type R[G/Y]CBV-UV....LED risk group 3 according to DIN EN 62471:2009
2.4 Operating and function controls
2.4.1 Connection panel on the lamp
module
The following connectors are located on the lamp
module (Fig. 4):
1Increment connection socket
2 USB connection socket
3 Remote connection socket
4 Breakout connection socket
5Power connection socket
6 CAN connection socket
Fig. 4 Connection panel on the lamp
module

DESCRIPTION OF THE DEVICE
ZEISS Operating and function controls Colibri 7
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2.4.2 LED Trigger Box for Colibri 7
(optional)
The LED Trigger Box is only needed if Colibri 7 is to
be controlled via a third-party software.
The front of the LED Trigger Box contains the
following elements (Fig. 5):
1Power indicator light
2Digital off / on selection button
36x trigger sockets (Digital In) for the LEDs to
be switched: UV, V, B, C, G/Y, R
4Filter socket
5Reserve socket
66x trigger sockets (Analog In) for the LEDs
to be switched: UV, V, B, C, G/Y, R
7Analog off / on selection button
The rear of the LED Trigger Box contains the
following elements (Fig. 5):
8Output of connection cable to Colibri 7
(to Breakout connection socket)
Function and description of the selection buttons and trigger sockets
Selection buttons
Digital: on The LED can only be switched on via the trigger sockets (Fig. 5/3).
Digital: off The LED can only be switched on via the control panel or the CAN interface.
Analog: on The LED intensity can only be adjusted via the trigger sockets (Fig. 5/6).
Analog: off The LED intensity can only be adjusted via the control panel or the CAN interface.
Trigger sockets
Digital In TTL level
Analog In 0 to 10 V
Fig. 5 LED Trigger Box for Colibri 7 (front
and rear side)

DESCRIPTION OF THE DEVICE
Colibri 7 Operating and function controls ZEISS
02/2017 423052-7344-001 15
2.4.3 Control panel for Colibri 7
(optional)
The control panel has the following operating and
function controls (Fig. 6):
1 Control dial for brightness with pushbutton
to select the LED to be controlled
2Settings button
3 Standby button
4Shutter button
5 Brightness control of all activated LEDs
switched on button
6 Display
UV LED button for wavelength 385 nm
V LED button for wavelength 430 nm
B LED button for wavelength 475 nm
C LED button for wavelength 511 nm
G LED button for wavelength 555 nm
Y LED button for wavelength 590 nm
R LED button for wavelength 630 nm
Functions of the control panel
Display / control element Function Description
Display Status display of LEDs or of
the device
−On / off state of LEDs
−Current brightness value of the switched
on LEDs in %
−Highlighting of controllable LEDs
−Error messages
LED buttons Switching one or several LEDs
on or off
LED on: LED button lights up
LED off: LED does not light up
Using the 7 LED buttons, up to 6 LEDs can be
switched on simultaneously.
Control dial Rotating function: Brightness
control
Control from dark to bright in steps of 1 % to
100 %
Push function: Selecting the
LEDs to be controlled
−Transit switching between switched-on
LEDs to activate for brightness control.
−A maximum of 6 LEDs can be switched on
simultaneously so that up to 6 LEDs can be
switched through.
−The LED which is currently controlled
flashes.
Fig. 6 Control panel for Colibri 7 –
operating and function controls

DESCRIPTION OF THE DEVICE
ZEISS Operating and function controls Colibri 7
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Display / control element Function Description
Settings button Setting of brightness for
buttons and display
Menu navigation as follows:
−Enable feature:
Press Settings button
−Adjusting brightness:
Turn the control dial
−Exit feature:
Press Settings button
Standby button Colibri 7 standby Activate standby:
−Press Standby button
In standby mode, the backlighting of all
buttons will be switched off, only the standby
button will light up.
All LEDs will be switched off.
Exit standby:
−Press Standby button
Shutter button Switching all LEDs on and off All LEDs (up to 6) will be switched on or off
simultaneously.
Brightness control of all activated
LEDs button
Brightness control of all
activated LEDs switched on
Activating all switched-on LEDs for brightness
control
−Control takes place in an adjusted ratio
relative to the wavelengths of the LEDs
−Control:
Rotate the control dial
- Default values of individual
LEDs
20 % after initialization and activation of the
LEDs
- Intensity value memory In Standby mode:
−Set brightness will be stored after switching
off the device, i.e. when the device is
switched on again, the previously selected
value will be restored.
After deenergizing the device:
−Default values are set.

START-UP
Colibri 7 Terminal diagrams ZEISS
02/2017 423052-7344-001 17
3START-UP
The Colibri 7 solid-state light source consists of the following components:
−Colibri 7 lamp module (available in 4 variations, incl. SPU65-102 desktop power supply unit)
−Control panel for Colibri 7 (optional)
−LED Trigger Box for Colibri 7 (optional)
The device can be commissioned by the customer.
The lamp module of the Colibri 7 solid-state light source is delivered completely assembled in accordance
with the equipment ordered (e.g. RGB-UV variation with 4 excitation lines).
3.1 Terminal diagrams
3.1.1 Terminal diagram for stand-alone operation
Fig. 7 Terminal diagram for stand-alone operation

START-UP
ZEISS Terminal diagrams Colibri 7
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3.1.2 Terminal diagram with PC and motorized microscope
SVB 1 signal distribution box optional, only for fast acquisition
CAN / USB optional:
CAN for control via ZEN 2.3 (blue edition) ZEISS software
USB for control via third-party software
Fig. 8 Terminal diagram with PC and motorized microscope for fast acquisition

START-UP
Colibri 7 Unpacking Colibri 7 ZEISS
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3.2 Unpacking Colibri 7
The lamp module, the desktop power supply unit and other optional accessories are supplied separately
in customary packaging.
•Remove all parts from the packaging.
•Check them for completeness according to the delivery note.
3.3 Attaching Colibri 7 to the microscope and connecting it
The lamp module is operated via the reflected light mount of the microscope.
In case of microscope systems with laser application, the clamping screw (Fig. 9/4
) on the
reflected light mount for fastening the lamp module to the microscope is a safety screw and
may only be removed by ZEISS Service (not a customer interface). Otherwise there is a ris
k of
eye damage upon laser coupling.
In case of microscope systems without laser
applications proceed as follows:
•Attach dovetail (Fig. 9/1) of the lamp module
(Fig. 9/2) to the reflected light mount (Fig. 9/3).
•Tighten clamping screw (Fig. 9/4).
The lamp module does not require any adjustment.
Depending on equipment variation, the Colibri 7
can be controlled via the TFT display of the
microscope, the control panel, the LED Trigger Box
or via PC.
Fig. 9 Attaching the lamp module to the
microscope

START-UP
ZEISS Attaching Colibri 7 to the microscope and connecting it Colibri 7
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Depending in the existing microscope equipment, ensure the correct connection type as follows:
•Connect the connection cable of the desktop power supply unit (Fig. 10/7) to the Power connection
socket (Fig. 10/5) of the lamp module (Fig. 10/1).
•If used, connect the control panel (Fig. 10/8) with connecting cable to the Remote connection socket
(Fig. 10/2) of the lamp module.
•If used, connect the LED Trigger Box (Fig. 10/9) with connecting cable to the Breakout connection
socket (Fig. 10/3) of the lamp module.
•If the microscope has a TFT display, connect the CAN connection socket (Fig. 10/4) of the lamp
module via the CAN cable to the microscope stand.
•If Colibri 7 is to be controlled via a third-party software on the PC, connect the USB connection socket
(Fig. 10/6) of the lamp module via USB cable to the PC.
Fig. 10 Connecting the lamp module and components