Zeiss Axiolab 5 User manual

Operating Manual
Axiolab 5
Upright Microscope for Routine and Entry-level Research
Microscope for Routine and Entry-Level Research

ZEISS Copyright / Trade Marks Axiolab 5
2 430037-7444-001 05/2019
Knowledge of this manual is essential for the operation of the instrument. Please familiarize yourself with
the contents of this manual and pay special attention to instructions concerning safe operation of the
instrument.
The specifications are subject to change; the manual is not covered by an update service.
© Unless expressly authorized, dissemination and duplication of this document as well as
commercial exploitation and communication of its contents are not permitted. Persons in
contravention of this copyright are liable to pay compensation for damages.
All rights reserved, including rights created by patent grant or registration of a utility model.
All names of companies and products mentioned in this manual may be trademarks or registered
trademarks. Third party products are cited for information purposes only and this does not represent
approval or recommendation of these products.
Carl Zeiss Microscopy GmbH assumes no liability for the performance or use of such products.
Published by Carl Zeiss Microscopy GmbH
Carl-Zeiss-Promenade 10
07745 Jena, Germany
[e-mail]
www.zeiss.com/microscopy
Carl Zeiss Suzhou Co., Ltd.
Modern Industrial Square 3-B, No.333
XingPu Road SIP
215126 Suzhou, China
Order No.: 430037-7444-001
Date of issue: Version 6 – 15 May 2019

Axiolab 5 Contents / List of Illustrations ZEISS
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CONTENTS
Page
1INTRODUCTION ................................................................................................... 8
1.1 Notes on instrument safety .......................................................................................... 8
1.2 Warning labels on the microscopes............................................................................ 12
1.3 Notes on the warranty................................................................................................ 14
2DESCRIPTION OF THE INSTRUMENT................................................................. 15
2.1 Intended use................................................................................................................ 15
2.2 Technical data.............................................................................................................. 17
2.3 Interface diagram........................................................................................................ 20
2.4 Control and functional elements on the microscope................................................. 21
2.4.1 Stand models................................................................................................................. 21
2.4.2 Axiolab 5 stand, Bio-TL................................................................................................... 22
2.4.3 Axiolab 5 stand, Bio-TL/FL............................................................................................... 24
2.4.4 Axiolab 5 stand, Pol-TL ................................................................................................... 26
2.4.5 Axiolab 5 stand, Pol-TL/conoscopy .................................................................................. 28
2.4.6 Axiolab 5 stand, Pol-TL/RL............................................................................................... 30
2.4.7 Axiolab 5 stand, Mat-TL/RL ............................................................................................. 32
2.4.8 Functions of stands keys and display elements................................................................. 34
2.5 Control and functional elements on microscope components.................................. 35
2.5.1 Binocular tubes/photo tubes ........................................................................................... 35
2.5.2Microscope stages.......................................................................................................... 38
2.5.3 Filter mount on luminous-field diaphragm operating ring for filter 32x4 mm.................... 40
2.5.4 Condensers.................................................................................................................... 40
2.5.5 Filter slider for reflected light stand................................................................................. 41
2.5.6 Reflector turret with 4 positions...................................................................................... 41
2.5.7 Low-power system for objectives 2.5x/4x ........................................................................ 41
2.5.8 Polarizer......................................................................................................................... 42
2.5.9 Nosepiece with objectives............................................................................................... 42
2.5.10 Microscope operating modes.......................................................................................... 43
2.5.11 Axiocam 202 mono/208 color controls and connectors ................................................... 49
2.5.12 OSD functionality with Axiocam 202 mono/208 color...................................................... 50
3START-UP........................................................................................................... 51
3.1 Mounting standard components................................................................................ 51
3.1.1 Unpacking and setting up microscope stand ................................................................... 51
3.1.2 Attaching the binocular tube/photo tube ........................................................................ 53
3.1.3 Inserting eyepieces or auxiliary microscope or pinhole diaphragm .................................... 54
3.1.4 Screwing in objectives .................................................................................................... 55
3.1.5 Inserting and removing P&C reflector modules in/from the reflector turret ....................... 56
3.1.6 Mounting a mechanical stage......................................................................................... 57
3.1.7Mounting the Pol rotary stage ........................................................................................ 59

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3.1.8 Mounting the condenser.................................................................................................62
3.1.9 Mounting the darkfield condenser...................................................................................63
3.1.10 Mounting or replacing the 35 W halogen lamp or the 10 W LED illuminator for
transmitted light .............................................................................................................64
3.1.11 Mounting or replacing the 35 W halogen lamp or the 10 W LED illuminator for reflected
light ...............................................................................................................................67
3.1.12 Installing or replacing the LED modules for reflected light fluorescence.............................70
3.1.13 Mounting the Axiocam 202 mono or Axiocam 208 color .................................................72
3.2 Mounting optional components .................................................................................73
3.2.1 Mounting the light intensive co-observer unit ..................................................................73
3.2.2 Mounting polarizer D or filter holder ...............................................................................73
3.2.3 Mounting and centering the low-power system for the objectives 2.5x/4x ........................74
3.2.4 Inserting the modulator disk in the condenser 0.9 BF Pol..................................................75
3.3 Connecting to the power supply.................................................................................75
3.4 Switching the microscope on/off................................................................................76
3.5Using the Light Manager function..............................................................................77
3.6 Default factory settings of the microscope ................................................................78
4OPERATION ........................................................................................................79
4.1 Default setting of the microscope...............................................................................79
4.1.1 Setting the inter-pupillary distance on the binocular tube .................................................79
4.1.2 Setting the viewing height ..............................................................................................79
4.1.3 Adjusting for ametropia (user's visual impairment) when using eyepiece reticles ...............80
4.2 Illumination and contrast methods in transmitted light............................................81
4.2.1 Configuring transmitted light brightfield microscopy using the KÖHLER method...............81
4.2.2 Configuring transmitted light darkfield microscopy using the KÖHLER method .................84
4.2.3 Configuring transmitted light phase contrast microscopy .................................................87
4.2.4 Configuring transmitted light polarization microscopy......................................................89
4.2.5 Configuring transmitted light polarization with the conoscopy stand................................98
4.2.6 Configuring transmitted light polarization for conoscopic observation – determining the
optical character of crystals ...........................................................................................109
4.3 Illumination and contrast methods in reflected light ..............................................112
4.3.1 Configuring reflected light brightfield microscopy using the KÖHLER method.................112
4.3.2 Configuring reflected light darkfield microscopy ............................................................114
4.3.3 Configuring reflected light polarization – Proof of bireflectance and reflexion
pleochroism..................................................................................................................115
4.3.4 Setting reflected light fluorescence................................................................................116
5CARE, FUSE REPLACEMENT AND SERVICE......................................................119
5.1 Instrument care..........................................................................................................119
5.2 Instrument maintenance ...........................................................................................120
5.2.1 Checking the instrument...............................................................................................120
5.2.2 Replacing the fuses in the stand ....................................................................................120
5.3 Troubleshooting.........................................................................................................121

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5.3.1 Microscope .................................................................................................................. 121
5.3.2 Axiocam 202/208......................................................................................................... 124
5.4 Maintenance and repair............................................................................................ 126
5.5 Firmware update....................................................................................................... 126
6APPENDIX ........................................................................................................ 127
6.1 List of abbreviations ................................................................................................. 127
6.2 Index .......................................................................................................................... 129
6.3 Industrial property rights.......................................................................................... 132
6.4 System overview....................................................................................................... 132
LIST OF ILLUSTRATIONS
Fig. 1-1 Warning labels on the Axiolab 5 stand for transmitted light and reflected light................... 12
Fig. 1-2 Warning labels on the Axiolab 5 stand for transmitted light ............................................... 13
Fig. 2-1 Interface diagram (Example: Axiolab 5 Mat-TL/RL stand) .................................................... 20
Fig. 2-2 Axiolab 5 stand, Bio-TL ..................................................................................................... 23
Fig. 2-3 Axiolab 5 stand, Bio-TL/FL ................................................................................................. 25
Fig. 2-4 Axiolab 5 stand, Pol-TL ..................................................................................................... 27
Fig. 2-5 Axiolab 5 stand, Pol-TL/Conoscopy.................................................................................... 29
Fig. 2-6 Axiolab 5 stand, Pol-TL/RL................................................................................................. 31
Fig. 2-7 Axiolab 5 stand, Mat-TL/RL ............................................................................................... 33
Fig. 2-8 Binocular photo tube 30°/23 with fixed graduation 50:50.................................................. 35
Fig. 2-9 Binocular photo tube 20°/23 with toggle graduation 100:0/0:100 ..................................... 35
Fig. 2-10 Binocular photo tube 30°/23 with toggle graduation 100:0/0:100 ..................................... 36
Fig. 2-11 Binocular ergo photo tube 20°/23 with vertical adjustment................................................ 37
Fig. 2-12 Mechanical stage 75x50 R with specimen holder ............................................................... 38
Fig. 2-13 Mechanical stage 75x50 R with specimen holder ............................................................... 38
Fig. 2-14 Mechanical stage for reflected light 75x30 R with specimen-holding plate.......................... 39
Fig. 2-15 Pol rotary stage ................................................................................................................ 39
Fig. 2-16 Filter mount on luminous-field diaphragm operating ring for filter d=32x4 mm .................. 40
Fig. 2-17 Condenser 0.9/1.25 BF, DF, Ph1, Ph2, Ph3 with modulator disk ......................................... 40
Fig. 2-18 Condenser 0.9/1.25 BF ..................................................................................................... 40
Fig. 2-19 4-position reflector turret.................................................................................................. 41
Fig. 2-20 Low-power system ........................................................................................................... 41
Fig. 2-21 Polarizers.......................................................................................................................... 42
Fig. 2-22 Nosepiece of the transmitted/ reflected light polarization stand with mount for
compensators................................................................................................................... 42
Fig. 2-23 Camera connection panel (rear side) ................................................................................. 49
Fig. 2-24 Camera operator panel (right side).................................................................................... 49

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Fig. 2-25 OSD menu, Home .............................................................................................................50
Fig. 3-1 Setting up the microscope.................................................................................................51
Fig. 3-2 Placing tools in the storage compartment ..........................................................................52
Fig. 3-3 Attaching the binocular tube .............................................................................................53
Fig. 3-4 Inserting eyepieces ............................................................................................................54
Fig. 3-5 Inserting the eyepiece reticle..............................................................................................54
Fig. 3-6 Screwing in objectives .......................................................................................................55
Fig. 3-7 Replacing the reflector module ..........................................................................................56
Fig. 3-8 Installing a mechanical stage .............................................................................................57
Fig. 3-9 Friction adjustment ...........................................................................................................58
Fig. 3-10 Replacing the snap-in Pol rotary stage, detachable Pol specimen guide and stage clips ........59
Fig. 3-11 Centering the Pol rotary stage ...........................................................................................60
Fig. 3-12 Centering objectives..........................................................................................................61
Fig. 3-13 Attaching the condenser ...................................................................................................62
Fig. 3-14 Mounting the darkfield condenser .....................................................................................63
Fig. 3-15 Removing the cover...........................................................................................................64
Fig. 3-16 Changing the halogen lamp ..............................................................................................64
Fig. 3-17 Removing the LED illuminator............................................................................................65
Fig. 3-18 Changing the LED illuminator in the adapter
......................................................................65
Fig. 3-19 Inserting the LED illuminator..............................................................................................66
Fig. 3-20 Removing the cover...........................................................................................................67
Fig. 3-21 Changing the halogen lamp ..............................................................................................67
Fig. 3-22 Swinging the loops out .....................................................................................................68
Fig. 3-23 Changing the LED illuminator ............................................................................................68
Fig. 3-24 Changing the LED illuminator in the adapter......................................................................69
Fig. 3-25 Removing the module holder.............................................................................................70
Fig. 3-26 Changing the LED modules ...............................................................................................70
Fig. 3-27 Mounting the Axiocam 202 mono or Axiocam 208 color....................................................72
Fig. 3-28 Mounting the polarizer D ..................................................................................................73
Fig. 3-29 Mounting the low-power system .......................................................................................74
Fig. 3-30 Modulator disk in condenser 0.9 H Pol...............................................................................75
Fig. 3-31 Power supply connector on the back of the stand ..............................................................75
Fig. 3-32 Mains switch on left side of microscope.............................................................................76
Fig. 3-33 Intensity/LM knob and illumination modes .........................................................................76
Fig. 4-1 Setting the inter-pupillary distance on the binocular tube ...................................................79
Fig. 4-2 Setting the viewing height on the binocular tube ...............................................................79
Fig. 4-3 Microscope settings in transmitted light brightfield microscopy...........................................82
Fig. 4-4 Setting the height stop on the condenser carrier ................................................................83
Fig. 4-5 Centering the darkfield stop on condenser, achromatic-aplanatic 0.9 H D Ph DIC................84
Fig. 4-6 Centering the annular phase diaphragm (light-colored, in the condenser) and the phase
ring (dark-colored, in the object)........................................................................................88
Fig. 4-7 Components for transmitted light polarization ...................................................................90
Fig. 4-8 Determining the polarization direction nγ'using a synthetic fiber as an example..................91

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Fig. 4-9 Schematic diagram of the color charts developed by Michel-Lévy ....................................... 93
Fig. 4-10 Components for circular polarization contrast ................................................................... 96
Fig. 4-11 Axiolab 5 for transmitted light conoscopy.......................................................................... 98
Fig. 4-12 Determining optical character ......................................................................................... 100
Fig. 4-13 Components for transmitted light polarization on the conoscopy stand............................ 102
Fig. 4-14 Determining the polarization direction nγ'using a synthetic fiber as an example ............... 103
Fig. 4-15 Schematic diagram of the color charts according to Michel-Lévy ...................................... 105
Fig. 4-16 Components for circular polarization contrast on conoscopy stand................................... 108
Fig. 4-17 Axiolab 5 for transmitted light conoscopy........................................................................ 110
Fig. 4-18 Determining the optical character ................................................................................... 111
Fig. 4-19 Microscope settings in reflected light brightfield microscopy ............................................ 113
Fig. 4-20 Components for reflected light fluorescence ................................................................... 118
Fig. 5-1 Replacing the fuses in the stand...................................................................................... 120

INTRODUCTION
ZEISS Notes on instrument safety Axiolab 5
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1INTRODUCTION
1.1 Notes on instrument safety
The Axiolab 5 microscopes were engineered, manufactured and tested in accordance with the
DIN EN 61010-1 (IEC 61010-1) and IEC 61010-2-101 safety standards for electrical measuring, control
and laboratory equipment.
The microscopes fulfill the requirements as stated in Directive 98/79/EC on in vitro diagnostic medical
devices (IVDD) and bear the marking.
The present manual contains information and safety warnings with which the operator must comply.
The following warning, instructional and informational symbols are used in this manual:
CAUTION
Warns the operator of a possible danger.
CAUTION
Warns the operator of hot surfaces.
CAUTION
Warns the operator of the emission of UV radiation.
CAUTION
Warns the operator of the emission of LED radiation.
CAUTION
Warns the operator to disconnect the instrument from the power supply before opening or
doing any work on the instrument.
ATTENTION
This symbol indicates the risk of damage to the instrument or the system.
NOTE
This symbol indicates information or instructions which must be followed especially carefully.

INTRODUCTION
Axiolab 5 Notes on instrument safety ZEISS
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Axiolab 5 microscopes and their original accessories may be used only for the microscopy procedures
described in this manual.
Compliance with the following instructions is mandatory:
CAUTION
The microscope may only be plugged into an electrical outlet equipped with a safety contact.
The safety feature must not be disabled by using an extension cord which does not have a
protective ground conductor.
CAUTION
Whenever it becomes apparent that any of the safety mechanisms are
out of order, the
microscope must be switched off and secured against any inadvertent use.
Please contact the ZEISS Service Department or the Carl Zeiss Microscopy Service before
switching the microscope on again.
CAUTION
The microscopes are equipped with integrated power supply units, which adapt to line
voltages ranging from 100 V to 240 V and frequencies ranging from 50 to 60 Hz. No voltage
adjustment is required on the microscopes themselves.
CAUTION
Always disconnect the microscope from the power supply before you open it
or change a
fuse!
M
ake sure that the fuses are suitable for the applied nominal current. Never use any
makeshift fuses and do not short-circuit the fuse holders.
CAUTION
The microscopes do not have any special safety devices to protect users from acid or from
potentially infectious, toxic, radioactive or other samples that may be
hazardous to your
health. Compliance with all statutory requirements, especially national accident prevention
regulations, is required when handling such samples.
CAUTION
Only authorized personnel are permitted to operate the instruments. The personnel must have
been instructed about and be aware of the risks involved in using the microscope. The Axiolab
is a precision instrument whose functionality
can easily be damaged or even destroyed when
handled improperly.

INTRODUCTION
ZEISS Notes on instrument safety Axiolab 5
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CAUTION
Operating the instrument in an area with a potentially explosive atmosphere is prohibited.
It may be operated only on stable, non-flammable surfaces.
Specimens must be disposed of appropriately in accordance with applicable statutory
regulations and internal work instructions.
CAUTION
The immersion fluid Immersol 518 Ncan cause skin irritations. Avoid any contact with skin,
eyes and clothes. Read the safety data sheets on Immersol 518 N, Immersol 518 Fand
Immersol Wcarefully. If skin contact occurs, wash off immediately using lots of water and
soap.
If eye
contact occurs, flush with water immediately for at least 5 minutes. Seek medical
assistance if irritation continues.
CAUTION
Dispose of immersion fluid Immersol 518 Nappropriately: Do not allow it to contaminate
surface water or enter drains or the sewage system.
CAUTION
Do not bring any flammable or easily combustible materials into the light beam.
CAUTION
This device belongs to LED Risk Group 2 as specified in IEC 62471 and emits LED radiation.
Never look into the LED beam of the illuminating device –
either with or without optical
instruments. Failure to comply with this warning may result in eye injuries!
ATTENTION
The manufacturer assumes no liability for any use of the microscope, its
assemblies or
individual parts if these are used in in a manner or for any purpose other than those described
in this operating manual. This also applies to any maintenance or repair work not performed
by authorized maintenance and repair personnel.
Moreover, the warranty and any claims
thereunder will be voided.
ATTENTION
Dirt and dust may affect the microscope’s performance. Protect it by using a dust cover when
it is not in use. Always make sure that the instrument is switched off before covering it up.

INTRODUCTION
Axiolab 5 Notes on instrument safety ZEISS
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ATTENTION
Always position equipment in a manner that permits easy disconnection from the power supply.
ATTENTION
Do not use a power supply cable with an inadequate rating.
ATTENTION
Closing or covering the ventilation slits may result in heat accumulation which could damage
the instrument and even start a fire. Ensure that the ventilation slits
are always kept open,
uncovered and clear. Do not insert or drop anything into them or let anything fall into them.
ATTENTION
Do not dispose of defective microscopes in the household waste. Comply with the applicable
statutory regulations for their disposal.
Specimens must also be disposed of appropriately
in accordance with applicable statutory
regulations and internal work instructions.

INTRODUCTION
ZEISS Warning labels on the microscopes Axiolab 5
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1.2 Warning labels on the microscopes
NOTE
Warning label: Hot surface!
Affixed to all stands with transmitted light halogen illumination.
Fig. 1-1 Warning labels on the Axiolab 5 stand for transmitted light and reflected light

INTRODUCTION
Axiolab 5 Warning labels on the microscopes ZEISS
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Fig. 1-2 Warning labels on the Axiolab 5 stand for transmitted light

INTRODUCTION
ZEISS Notes on the warranty Axiolab 5
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1.3 Notes on the warranty
The manufacturer guarantees that the instrument is free of any material and workmanship defects upon
delivery. If you become aware of any deficiencies, please contact us immediately and take all necessary
precautions in order to avoid further damage. Upon notice of deficiencies, the manufacturer may choose
to correct the deficiencies or to deliver a defect-free instrument at his discretion. Defects due to ordinary
wear and tear (especially on wearing parts) and to improper handling are not covered by our warranty.
The manufacturer is not liable for damage to the instrument due to incorrect operation, negligence or
any other manipulation of the instrument, in particular due to the removal or replacement of instrument
parts or due to the use of accessories from other manufacturers. This will immediately void the warranty.
No maintenance or repair work, except for the instances mentioned in the manual, may be performed on
the microscopes. Only ZEISS service personnel or personnel authorized especially by ZEISS may perform
repair work on the microscopes. Should your instrument malfunction, please contact the ZEISS
Microscopy Service Department (see page 126) or the ZEISS agency assigned to your country.

DESCRIPTION OF THE INSTRUMENT
Axiolab 5 Intended use ZEISS
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2DESCRIPTION OF THE INSTRUMENT
2.1 Intended use
Axiolab 5 microscopes were designed as all-purpose microscopes for biological and medical applications
as well as materials analyses.
Depending on the microscope stand selected, they may also be used as true transmitted or reflected light
microscopes or as combined transmitted/reflected light fluorescence microscopes.
Typical biomedical applications of Axiolab 5 microscopes include:
−medical analysis in laboratories, clinics and medical practices
−science and research (colleges, universities) in the fields of medicine and biology
−industrial applications (pharmacology, food technology)
−analysis of human blood and tissue samples.
Axiolab 5 microscopes are typically used for materials analysis in the following areas, among others:
−metallographic laboratories
−automotive industry
−microsystems engineering
−geoscientific institutes
−mineral exploration industry
Depending on the instrument configuration, the following microscopy and contrasting techniques can be
used:
Transmitted light
Reflected light
−
Brightfield (BF)
−
Brightfield (BF)
−
Darkfield (DF)
−
Darkfield (DR)
−
Phase contrast (Ph)
−
Polarization (Pol)
−
Polarization (Pol)
−
Fluorescence (FL)
−
Polarization (Conoscopy)
−
Differential Interference Contrast (DIC)
−
Polarization (C-Pol)
−
Circular differential interference
contrast/total interference contrast
(C-DIC/TIC)
The binocular photo tubes and suitable adapters permit one microscope camera, one reflex camera or
one digital/video camera to be attached for documentation purposes.
The Axiolab 5 was specifically designed and developed for ergonomic use in lengthy routine applications,
e.g. hematological, histological and cytological laboratory analyses.
Using a ZEISS Axiocam 202 mono or Axiocam 208 color camera, the microscope can be connected to an
external HD monitor via an HDMI connection or to a PC/laptop via a USB connection to control camera
functionality. Advanced analysis functions for recorded images are available if the ZEN software is
installed on the PC. Furthermore, the camera functions can be controlled via WiFi connection from a
tablet, PC or smartphone using the Labscope/Matscope app.

DESCRIPTION OF THE INSTRUMENT
ZEISS Intended use Axiolab 5
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The ergonomic design elements of the microscopes include:
−vertically adjustable, swivel-type and swivel/vertically adjustable ergo tubes
−Latex free, skin-friendly surfaces on the binocular section of the tubes, control elements and stand
−stage drives whose height and friction can be adjusted
−optional use of fine focusing knobs in standard form or as a jog/shuttle knob
−special ergonomic arrangement of the four main control elements: focusing drive, stage drive, light
manager control, and snap image button for comfortable operation

DESCRIPTION OF THE INSTRUMENT
Axiolab 5 Technical data ZEISS
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2.2 Technical data
Dimensions (length x width x height)
Axiolab 5 basic microscope stand
without tube (430037-9011-000) ...............................................approx. 304 mm x 210 mm x 357.5 mm
The other stand models differ slightly in depth and significantly in height, depending on the tube used.
An overview of viewing heights (eyepoint heights) of the various tubes can be found on page 19.
An estimate of the height of the stand with the respective tube can be obtained by adding:
−10 mm to the viewing height in the lower position of the binocular section for tubes with a fixed
viewing angle
−10 mm to the viewing height of the upper limit for ergo tubes
Weight
Axiolab 5 microscope stand (depending on version and accessories)............................. approx. 8 to 20 kg
Ambient conditions
Shipping (in packaging):
Permissible ambient temperature................................................................................... -40 to +70 °C
Permissible humidity (without condensation)..........................................................max. 75% at 35 °C
Storage:
Permissible ambient temperature.................................................................................. +10 to +40 °C
Permissible humidity (without condensation)..........................................................max. 75% at 35 °C
Operation:
Permissible ambient temperature.................................................................................. +10 to +40 °C
Permissible relative humidity (without condensation) .............................................. max. 75% at 35 °C
Highest permitted altitude of use.................................................................................... max. 2000 m
Air pressure ........................................................................................................800 hPa to 1060 hPa
Degree of pollution .......................................................................................................................... 2
Operational specifications
Operational area.................................................................................................................Closed rooms
Protective class .......................................................................................................................................I
Protection type ................................................................................................................................IP 20
Electrical safety............................................................in accordance with DIN EN 61010-1 (IEC 61010-1)
............................................................................................. in compliance with CSA and UL regulations
Overvoltage category.............................................................................................................................II
RFI suppression....................................................................................... complies with EN 55011 Class B
Noise immunity ......................................................................................complies with DIN EN 61326/A1
Mains voltage for the Axiolab 5........................................................................................... 100 to 240 V
Mains frequency................................................................................................................... 50 to 60 Hz
Power consumption of the Axiolab 5............................................................................................100 VA
Fuses in compliance with IEC 127
Axiolab 5 microscope stand ............................................................................. 2 x T 3.15 A/H, 5x20 mm

DESCRIPTION OF THE INSTRUMENT
ZEISS Technical data Axiolab 5
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Light sources
LED illumination with transmitted light/reflected light
Power consumption........................................................................................................... max. 10 W
Adjustment of light source.............................................................. continuous approx. 10 to 800 mA
Halogen lighting with transmitted light/reflected light
Power consumption........................................................................................................... max. 35 W
Adjustment of light source..................................................................continuous, approx. 0.5 to 12 V
LED lighting with reflected light fluorescence with replaceable LED modules
Wavelengths optional ..................................................................................... 385, 470, 565, 625 nm
LED classification ......................................................................................LED Risk Group 2 to IEC 62471
Axiolab 5
Stand with manual stage focusing
Coarse focusing............................................................................................ approx. 4 mm/revolution
Fine focusing ................................................................approx. 0.4 mm/revolution; 4 µm scale interval
Lifting range..........................................................................depending on the stand, 15 mm/30 mm
Height stop ..................................................................................................................factory pre-set
Condenser 0.9/1.25 BF with optional
modulator disk ........................................................................................ for brightfield, darkfield and
.........................................................................................................................phase contrast 1, 2, 3
Manual objective change........................ depending on the nosepiece, 5x BF Pol or 5x BF DF, M27, coded
Manual reflector module change ...................................................... for 4-position reflector turret, coded

DESCRIPTION OF THE INSTRUMENT
Axiolab 5 Technical data ZEISS
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Viewing height and tube angle
Order No. Binocular tube Viewing
angle
Adjustment Viewing
height* in mm
425520-9000-000 Binocular tube 30°/23 30° - None - 449 / 485
425520-9010-000 Binocular photo tube 30°/23 (50:50) 30° - None - 449 / 485
425520-9020-000 Binocular photo tube 30°/23 (100:100)
Bio
30° - None - 449 / 485
425520-9030-000 Binocular photo tube 20°/23 (100:100) 20° - None - 442 / 481
425520-9050-000 Binocular ergo tube 15°/23 (50/50),
telescopic, height, upright image
15° Height,
telescopic
410 - 509
425520-9090-000 Binocular tube 20°/23 20° 442 / 481
425520-9100-000 Binocular photo tube 20°/23 Pol
(100:100)
20° 442 / 481
425512-0000-000 Binocular ergo tube 20°/23 (100/100),
reverse image, 44 mm height
20° Height 457 - 574
* Viewing heights:
Tubes with fixed viewing angle without ergo function:
Binocular part, lower/upper e.g. 442 / 481 →442 to 481 mm
Angle- and vertically adjustable ergo tubes:
Binocular part, lower/upper e.g. 457 / 574 →457 to 574 mm
All specifications are for an inter-pupillary distance of 65 mm.

DESCRIPTION OF THE INSTRUMENT
ZEISS Interface diagram Axiolab 5
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2.3 Interface diagram
The following figure shows a diagram of the interfaces of the microscope stand. The Axiolab 5 Mat-TL/RL
stand is used here as an example.
Fig. 2-1 Interface diagram (Example: Axiolab 5 Mat-TL/RL stand)