Zeiss Axio Imager User manual

Axio Imager Upright Microscope
Operating Manual

Carl Zeiss Copyright / Trademarks Axio Imager
2 430000-7344-001 M70-2-0020 e 06/2009
Knowledge of this manual is required for the operation of the instrument. Would you therefore please
make yourself familiar with the contents of this manual and pay special attention to hints concerning safe
operation of the instrument.
The specifications are subject to change; the manual is not covered by an update service.
© Unless expressly authorized, forwarding and duplication of this document and the software
package, as well as utilization and communication of its contents are not permitted. Violations will
entail an obligation to pay compensation.
All rights reserved in the event of granting of patents or registration of a utility model.
All names of companies and products mentioned in this manual may be trademarks or registered
trademarks. Quoting of product names is for information only and does not represent any trademark
misuse.
Carl Zeiss AG is not liable for the performance or the use of these products.
Issued by: Carl Zeiss MicroImaging GmbH
P.O.B. 4041, 37030 Göttingen, Germany
Phone: [phone]
Fax: [phone]
E-mail: [email protected]
www.zeiss.de
Document number: M70-2-0020 e
Date of issue: Version 2 - 06/12/2009

Axio Imager Contents Carl Zeiss
M70-2-0020 e 06/2009 430000-7344-001 3
CONTENTS
Page
1Introduction........................................................................................................................7
1.1 Notes on instrument safety ...................................................................................................7
1.2 Warning stickers .................................................................................................................10
1.3 Notes on warranty ..............................................................................................................12
1.4 Overall view of Axio Imager - manual ..................................................................................13
1.5 Overall view of Axio Imager - motorized..............................................................................14
2Instrument Decription......................................................................................................15
2.1 Name and intended use ......................................................................................................15
2.2 Instrument description and main features............................................................................15
2.3 Equipment and compatibility table ......................................................................................17
2.4 System overview of Axio Imager..........................................................................................19
2.5 Objectives ...........................................................................................................................26
2.6 Eyepieces ............................................................................................................................27
2.7 Stage micrometers and eyepiece reticles..............................................................................27
2.8 Technical data.....................................................................................................................29
3Start-up .............................................................................................................................32
3.1 Unpacking and installing the microscope.............................................................................32
3.2 Attaching or changing binocular tube or phototube............................................................33
3.3 Mounting the tube lens turret .............................................................................................34
3.4 Inserting eyepieces and auxiliary microscope........................................................................34
3.4.1 Inserting the eyepiece reticle ...............................................................................................35
3.4.2 Compensation of ametropia when eyepiece reticles are used ..............................................35
3.4.3 Inserting the fold-over eyecups............................................................................................35
3.5 Setting the interpupillary distance on the binocular tube .....................................................36
3.6 Setting the viewing height ..................................................................................................36
3.7 Fitting components to the camera port of the binocular phototube.....................................37
3.8 Screwing in objectives .........................................................................................................38
3.9 Attaching or changing the condenser..................................................................................38
3.10 Replacing the DIC prism on the universal condenser............................................................39
3.11 Inserting reflector turret, compensator mount 6x20 or 4-position modulator turret .............40
3.12 Changing the DIC prism of the modulator turret .................................................................40
3.13 Changing the stage carrier ..................................................................................................41
3.14 Setting stops for the adjustment range of the stage carrier dovetail guide ...........................41
3.15 Setting the dovetail guide of the stage carrier......................................................................42
3.16 Attaching the condenser carrier to the transmitted-light/reflected-light stage carrier............43
3.17 Changing the fine-drive knob or the fine-drive disk on the stand.........................................43
3.18 Equipping 2-position filter wheels 2x, discrete .....................................................................44
3.18.1 Filter wheel, manual............................................................................................................44
3.18.2 Filter wheel, 2-position, motorized ......................................................................................45

Carl Zeiss Contents Axio Imager
4 430000-7344-001 M70-2-0020 e 06/2009
3.19 Installing and removing P&C reflector modules....................................................................46
3.19.1 Installing a module..............................................................................................................46
3.19.2 Removing a module ............................................................................................................ 46
3.20 Changing the filter set in the reflector module FL P&C ........................................................46
3.21 Changing the beam splitter in the reflector module FL P&C.................................................48
3.22 Activating/deactivating the high-performance focus............................................................49
3.23 Mounting the TFT display to the motorized stand................................................................50
3.24 Attaching the TFT display to the docking station .................................................................50
3.25 Installing the focus linear sensor..........................................................................................51
3.26 Assembling the multidiscussion equipment .........................................................................52
3.27 Connecting to power..........................................................................................................54
3.27.1 Manual stand......................................................................................................................54
3.27.2 Motorized stand..................................................................................................................54
3.28 HAL 100 halogen illuminator...............................................................................................55
3.28.1 Attaching the HAL 100 halogen illuminator.........................................................................55
3.28.2 Separate power supply of the HAL 100 halogen illuminator in reflected light ......................56
3.28.3 Adjusting the HAL 100 halogen illuminator.........................................................................57
3.28.4 Replacing the HAL 100 halogen lamp..................................................................................58
3.29 Installing the LED illuminator for transmitted light ...............................................................59
3.30 Attachment lamp VIS-LED for transmitted light ...................................................................61
3.30.1 Installing the attachment lamp VIS-LED ...............................................................................61
3.30.2 Adjusting the attachment lamp VIS-LED ..............................................................................62
3.31 HBO 100 illuminator ...........................................................................................................63
3.31.1 Inserting the HBO 103 W/2 mercury vapor short-arc lamp ................................................... 63
3.31.2 Attaching the HBO 100 illuminator .....................................................................................63
3.31.3 Aligning the HBO 100 illuminator........................................................................................64
3.31.4 Installing the switching mirror for two illuminators (manual or motorized)........................... 65
3.32 Electrical connections on the rear side of the microscope ....................................................66
3.32.1 Manual stand......................................................................................................................66
3.32.2 Motorized stand..................................................................................................................66
3.33 Changing the mechanical stage ..........................................................................................67
3.33.1 Fixed mechanical stage 75x50 R..........................................................................................67
3.33.2 Rotary mechanical stage 75x50/240° R................................................................................67
3.33.3 Adjusting the travel range on ergonomic drive ....................................................................68
3.33.4 Removing and attaching additional sleeves.........................................................................69
3.33.5 Adjusting the smoothness (torque) of the two drive knobs of the ergonomic drive ..............69
3.33.6 Changing the specimen holder............................................................................................70
3.34 Rotary stage Pol ..................................................................................................................70
3.34.1 Removing rotary stage Pol...................................................................................................70
3.34.2 Attaching rotary stage Pol...................................................................................................70
3.34.3 Centering rotary stage Pol...................................................................................................71
3.34.4 Centering objectives............................................................................................................72
3.35 Installing Polarizer D, fixed ..................................................................................................73

Axio Imager Contents Carl Zeiss
M70-2-0020 e 06/2009 430000-7344-001 5
4Operation..........................................................................................................................74
4.1 Coded Axio Imager operation and function controls............................................................74
4.2 Axio Imager operation and function controls (motorized version).........................................82
4.3 Switching microscope and HAL 100 illuminator on/off.........................................................86
4.4 Switching the HBO 100 on/off ............................................................................................87
4.5 Binocular phototube 30°/25 mot. with two camera ports (2TV tube mot.)...........................87
4.6 Mechanical Stage 75x50 mot. CAN.....................................................................................89
4.6.1 Assembling Mechanical Stage 75x50 mot. CAN ..................................................................89
4.6.2 Connecting Mechanical Stage 75x50 mot. CAN .................................................................90
4.6.3 CAN/USB converter .............................................................................................................90
4.6.4 Docking station...................................................................................................................91
4.7 Functions of the Light Manager...........................................................................................92
4.7.1 Light Manager Mode: OFF...................................................................................................93
4.7.2 Light Manager Mode: CLASSIC ...........................................................................................93
4.7.3 Light Manager Mode: SMART .............................................................................................94
4.7.4 Light Manager of the coded microscope .............................................................................95
4.7.5 Light Manager and Dazzle Protection control on manual stands ..........................................95
4.7.6 Light Manager of motorized microscope .............................................................................96
4.8 Operating the motorized microscope via the touchscreen of the TFT display........................97
4.8.1 Screen layout ......................................................................................................................97
4.8.2 Menu structure .................................................................................................................100
4.8.3 Home page .......................................................................................................................101
4.8.4 Favorites page...................................................................................................................104
4.8.5 Microscope page...............................................................................................................104
4.8.6 Settings page ....................................................................................................................119
4.8.7 User page .........................................................................................................................136
4.8.8 Display page .....................................................................................................................144
4.9 Illumination and contrast methods ....................................................................................145
4.9.1 Setting transmitted-light brightfield according to KÖHLER.................................................145
4.9.2 Setting transmitted-light darkfield .....................................................................................148
4.9.3 Setting transmitted-light phase contrast ............................................................................151
4.9.4 Setting transmitted-light differential interference contrast (DIC).........................................153
4.9.5 Setting transmitted-light polarization for orthoscopic observation......................................156
4.9.6 Setting transmitted-light polarization for conoscopic observation – determining the
optical character of crystals ...............................................................................................164
4.9.7 Setting reflected-light brightfield.......................................................................................167
4.9.8 Setting reflected-light darkfield .........................................................................................170
4.9.9 Setting reflected-light DIC and reflected-light C-DIC..........................................................171
4.9.10 Setting reflected-light TIC..................................................................................................173
4.9.11 Setting epi-fluorescence ....................................................................................................176
4.9.12 Setting reflected-light polarization – Detection of bireflection and reflection
pleochroism ......................................................................................................................178

Carl Zeiss Contents Axio Imager
6 430000-7344-001 M70-2-0020 e 06/2009
5Care, Maintenance, Troubleshooting and SERVICE .....................................................180
5.1 Instrument care.................................................................................................................180
5.2 Instrument maintenance ...................................................................................................181
5.2.1 Performing checks.............................................................................................................181
5.2.2 Changing fuses on the manual stand ................................................................................181
5.2.3 Changing fuses on the VP232-2 CAN power supply .........................................................182
5.3 Troubleshooting................................................................................................................183
5.4 Spares, consumables and tools.......................................................................................... 186
5.5 Requesting service.............................................................................................................187
6Appendix ........................................................................................................................188
6.1 List of abbreviations ..........................................................................................................188
6.2 Index.................................................................................................................................190
6.3 Industrial property rights ...................................................................................................194

INTRODUCTION
Axio Imager Notes on instrument safety Carl Zeiss
M70-2-0020 e 06/2009 430000-7344-001 7
1INTRODUCTION
1.1 Notes on instrument safety
The Axio Imager microscopes have been designed, produced and tested in compliance with
DIN EN 61010-1 (IEC 61010-1) and IEC 61010-2-101 safety requirements for electrical measuring, control
and laboratory instruments.
The instruments meet the requirements of EC Directive IVDD 98/79/EC (In Vitro Diagnostic); they are
marked with the mark.
The instruments are to be disposed of in accordance with EU Directive 2002/96/EC (WEEE).
This operating manual includes information and warnings that must be observed by the user.
The following warning and information symbols are used in this manual:
NOTE
This symbol is a warning, which you must observe under all circumstances.
CAUTION
This symbol is a warning, which indicates a hazard to the instrument or instrument system.
CAUTION
This symbol is a warning, which indicates a hazard to the user of the instrument.
CAUTION
Hot surface!
CAUTION
UV radiation is emitted!
CAUTION
LED radiation ranging from 400 nm to 700 nm!
Do not look into the LED illuminator.
CAUTION
Disconnect the instrument from line power before opening it!
CAUTION
Crushing hazard

INTRODUCTION
Carl Zeiss Notes on instrument safety Axio Imager
8 430000-7344-001 M70-2-0020 e 06/2009
The Axio Imager microscopes, including original accessories, may only be used for the microscope
techniques described in this manual.
Particular attention must be paid to the following warnings:
The manufacturer cannot assume any liability for any other applications of the instrument
including those of individual modules or single parts. This also applies to all service or repair
work that is not carried out by authorized service personnel. In case of non-compliance, all
warranty claims shall be forfeited.
The power plug must be inserted in an outlet featuring a grounding (earth) contact. The
grounding effect must not be made ineffective by an extension cable that does not have a
protective ground wire.
If it is determined that protection measures are no longer effective, the instrument must be
switched off and safeguarded against inadvertent operation. Please contact a Zeiss service
agency or the Carl Zeiss Microscopy Service to repair the instrument.
On stands with motorized focusing drive, there is the risk of getting one's fingers jammed
between stage carrier and the base of the stand, when the stage is moved down. Therefore, do
not reach with your hands under the stage carrier.
The manual microscopes (Axio Imager.A2, .A2 LED, .A2m, .D2 and .D2m) are equipped with a
power supply integrated in the stand allowing line voltages to be used in the ranges 100 to
127 V and 200 to 240 V AC ±10 %, 50/60 Hz, without the voltage setting on the instrument
having to be changed. The motorized models (Axio Imager.M2, .M2m, .Z2 and .Z2m) are
powered through the separate power supply VP232-2 belonging to the stand. On this power
supply, too, the voltage setting need not be changed in the line voltage range
100 to 240 V ±10 %, 50/60 Hz.
The HBO 100 (ebq 100 dc) and XBO 75 (ebx 75 isolated) power supply units are designed for a
line voltage range from 100 to 240 V AC, 50/60 Hz, and automatically adapt to the applied line
voltage.
Before switching on the instrument, check whether it is suitable for the available line voltage.
Always disconnect the instrument from the power outlet before opening the instrument and
before changing the fuses. See Section 5.2.2.
Take care to ensure you only use fuses according to the information given in the Technical
Data. Use of makeshift fuses and short-circuiting of the fuse holders are not permitted.
The Axio Imager microscopes are not equipped with any special devices for protection from
substances that are corrosive, potentially infectious, toxic, radioactive, or other substances that
could be hazardous to health. Make sure to observe all legal regulations, particularly the
relevant national accident prevention regulations when handling such substances.
Do not put defective instruments in household waste. They have to be disposed of in
accordance with applicable legal provisions. Also, specimens need to be properly disposed of in
conformity with applicable legal provisions and in-house work instructions.

INTRODUCTION
Axio Imager Notes on instrument safety Carl Zeiss
M70-2-0020 e 06/2009 430000-7344-001 9
Gas-discharge lamps, e.g. HBO 50; HBO 100 or XBO 75, emit ultraviolet radiation, which can
cause burns to the eyes and skin. Therefore, never look directly into the light of these lamps
and avoid direct, unprotected incidence of their light on your skin. When using the microscope,
always use the protective devices belonging to the instrument (e.g. special attenuation filters or
the fluorescence shield). When they are hot, gas-discharge lamps are under high internal
pressure. Therefore, change them only when they have cooled down, and make sure to wear
protective gloves and a face guard.
When fluorescence filters are used, the heat protection filter for heat emitted by the
microscope illuminator must not be removed, since fluorescence filters are sensitive to heat,
and their performance could be impaired.
Avoid touching the hot lamp housing. Always pull the power plug before changing the lamps
and allow the instrument to cool down for some 15 minutes.
Dust and dirt may impair the instrument’s performance. Therefore, the instrument must be
protected from these influences as far as possible and covered with the dust cover when not in
use. Always check whether the instrument is switched off before you cover it.
Clogged or covered ventilation slats may lead to heat build-up that will damage the instrument
and, in extreme cases, cause a fire. Always keep the ventilation slats clear and ensure that no
objects enter the instrument through the ventilation slats.
The instruments may only be operated by trained personnel who must be aware of the possible
dangers involved with microscopy and the particular application concerned. The Axio Imager
microscopes are high-precision instruments that can be impaired in their performance or
destroyed when handled improperly.
The LED illuminator is a Laser Class 2M device. Do not look directly into the LED light.
Do not put defective instruments in household waste. They have to be disposed of in
accordance with applicable legal provisions.
Also, specimens need to be properly disposed of in conformity with applicable legal provisions
and in-house work instructions.
For stands equipped with motorized focusing drives, there is a risk of fingers getting crushed
in the working area when the microscope stage is being lowered.
−Do not reach into the area between the microscope stage / stage carrier / condenser
carrier and the stand base while the stage is being lowered.
−The automatic movement can be stopped by pressing the STOP button on the TFT
display. (See Fig. 1-2).

INTRODUCTION
Carl Zeiss Warning stickers Axio Imager
10 430000-7344-001 M70-2-0020 e 06/2009
Bei Verwendung eines Scanningtisches (ca. 4 kg Gewicht) muss zum Schutz des Fokussiertriebs
der Hochleistungsfokus aktiviert werden (siehe Seite 49). Probengewichte bis maximal 5 kg
können bei aktiviertem Hochleistungsfokus auf den Tisch aufgelegt werden.
Make sure to read the safety notes provided with Immersol 518 F®immersion oil.
Immersol 518 F®immersion oil irritates the skin. Avoid any contact with skin, eyes and clothing.
After inadvertent skin contact, wash the oil off with plenty of water and soap.
After inadvertent eye contact, immediately rinse the eye with plenty of water for at least five
minutes. If the irritation persists, consult a specialist.
Proper disposal of Immersol 518 F®immersion oil: Take care to ensure that the immersion oil
does not enter surface water or the sewage system.
1.2 Warning stickers
Fig. 1-1 "Crushing Hazard" warning sticker on
the base of motorized stands
Fig. 1-2 STOP button
The STOP button on the TFT display serves to switch off the movement of the focusing drive
instantaneously, so as to prevent a collision with the stage or the specimen placed on it.
Actuation of the STOP button causes the movement of the focusing drive to be halted immediately.

INTRODUCTION
Axio Imager Warning stickers Carl Zeiss
M70-2-0020 e 06/2009 430000-7344-001 11
Fig. 1-3 Warning stickers on the underside of the LED illuminator
Fig. 1-4 Warning stickers on the attachment lamp VIS-LED

INTRODUCTION
Carl Zeiss Notes on warranty Axio Imager
12 430000-7344-001 M70-2-0020 e 06/2009
1.3 Notes on warranty
The manufacturer guarantees that the instrument has no material or production defects when delivered.
You must inform us of any defects immediately and do everything to minimize any damage. If the
manufacturer is informed of such a defect, he is obligated to rectify it; it is his decision whether he does
this by repairing the instrument or by delivering an instrument free of any defect. No guarantee is
provided for defects caused by natural wear (wearing parts and consumables in particular) and improper
use.
The instrument manufacturer is not liable for damage caused by faulty operation, negligence or any other
tampering with the instrument, particularly the removal or replacement of instrument components, or
the use of accessories from other manufacturers. This forfeits all warranty claims.
With the exception of the work specified in this manual, no maintenance or repair of the Axio Imager
may be undertaken. Repairs may only be performed by Zeiss service staff or specially authorized
personnel. Should any defect occur with the instrument, please get in touch with your local Zeiss
representative.

INTRODUCTION
Axio Imager Overall view of Axio Imager - manual Carl Zeiss
M70-2-0020 e 06/2009 430000-7344-001 13
1.4 Overall view of Axio Imager - manual
1Eyepieces
2Binocular tube
3Reflected-light illuminator (HBO 100)
4Microscope stand, manual
5Transmitted-light illuminator (HAL 100)
6Mechanical stage
7Nosepiece
8Condenser
9Reflector turret

INTRODUCTION
Carl Zeiss Overall view of Axio Imager - motorized Axio Imager
14 430000-7344-001 M70-2-0020 e 06/2009
1.5 Overall view of Axio Imager - motorized
1Eyepieces
2Binocular phototube
3Reflected-light illuminator (HBO 100)
4Microscope stand, motorized
5Transmitted-light illuminator (HAL 100)
6TFT display
7Mechanical stage
8Nosepiece
9Condenser
10 Reflector turret

INSTRUMENT DECRIPTION
Axio Imager Name and intended use Carl Zeiss
M70-2-0020 e 06/2009 430000-7344-001 15
2INSTRUMENT DECRIPTION
2.1 Name and intended use
Manufacturer's designation: Axio Imager: Axio Imager MAT: Axio Imager LED:
Axio Imager.A2 Axio Imager.A2m Axio Imager.A2 LED
Axio Imager.D2 Axio Imager.D2m
Axio Imager.M2 Axio Imager.M2m
Axio Imager.M2p Axio Imager.Z2m
Axio Imager.Z2
The Axio Imager / Axio Imager MAT microscopes have been designed as universal microscopes for
applications in biology and medicine for the examination of blood and/or tissue specimens from the
human body, as well as for materials examinations.
They may also be used as true reflected-light microscopes or, if equipped with a transmitted-light
equipment, as combined reflected-light/transmitted-light microscopes.
Typical applications of the Axio Imager microscopes include, for instance:
−Medical examinations in laboratories (research), clinics and medical practices
−Science and research (colleges, universities) in the fields of medicine and biology
−Industrial applications (pharmacology, food technology)
Typical applications of the Axio Imager MAT microscopes include, for instance:
−Metallographic laboratories
−Vehicle industry
−Microsystems engineering
2.2 Instrument description and main features
With the advanced pyramid and modular design, the Axio Imager microscope incorporates time-tested
principles in microscope construction, thus ideally combining modern requirements made on design,
ergonomics, operating convenience and function with technical performance.
Depending on the instrument configuration, the following microscopy and contrasting techniques are
possible:
Transmitted light: Reflected light:
- Brightfield (H)
- Darkfield (D)
- Phase contrast (Ph)
- Differential Interference Contrast (DIC)
- Polarization contrast (Pol)
- Circular polarization
- Brightfield (H)
- Darkfield (D)
- Differential Interference Contrast (DIC)
- Differential Interference Contrast in circularly
polarized light (C-DIC)
- Polarization contrast (Pol)
- Fluorescence

INSTRUMENT DECRIPTION
Carl Zeiss Instrument description and main features Axio Imager
16 430000-7344-001 M70-2-0020 e 06/2009
The Axio Imager Microscope is available in ten stand versions. However, the scope of the equipment of
these stands is variable and can be customized to the user's requirements within the range of optionally
available microscope components.
Coded models:
Axio Imager.A2, A2m and A2 LED
Partially motorized models:
Axio Imager.D2, D2m
Motorized models:
Axio Imager.M2, M2m, M2p
Axio Imager.Z2 and Z2m
The binocular phototubes and suitable adapters permit one microscope camera, one reflex camera or one
digital / video camera to be attached for documentation purposes.

INSTRUMENT DECRIPTION
Axio Imager Equipment and compatibility table Carl Zeiss
M70-2-0020 e 06/2009 430000-7344-001 17
2.3 Equipment and compatibility table
Type of Stand
Component Option A2
LED A2 M2p M2 D2 Z2 A2 m
M2
m
D2
m Z2m
Manual + + - - + - + - + -
Stand Motorized - - + + O* + - + O* +
Coding PC readable + + + + + + + + + +
Coded O O O O O O O O O O
Tube lens turret Motorized - - O O - O - O - O
6 positions, coded O O O O O O O - O O
6 positions, motorized - - O O O O - + O O
6 positions, motorized ACR - - - - - O - - - O
Reflector turret
10 positions, motorized
ACR** - - - - O O - - O O
6 positions, coded POL O O - O O O O O O O
6 positions, coded HD DIC O O - O O O O O O O
6 positions, motorized HD
DIC - - - O - O - O - O
6 positions, motorized HD
DIC ACR -- - O - O - O - O
7 positions, coded HD O O + O O O O O O O
Objective nosepiece
7 positions, motorized HD - - - O - O - O - O
Manual O O O O O O O O O OModulator turret for
C-DIC/TIC Motorized***** - - - O - O - O - O
Modulator turret for
transmitted-light DIC Motorized***** - - - - - O - - - O
Attachable stage carrier with
condenser carrier
0 mm – 25 mm + + + + + O O O O O
Attachable stage carrier for
detachable condenser carrier
0 mm – 45 mm
O O O O O O O O O O
Attachable reflected-light
stage carrier 0 mm – 63 mm O O O O O O O O O O
Manual - + - - + - O O O O
Transmitted-light illumination Motorized - - - + - + - - - O
Transmitted-light LED - + O + O O O O O O O
Manual - + - O O O O O O O
Two-position transmitted-light
filter wheel Motorized - - - O - O - - - O
Manual*** O O O O O O + - + -
Reflected-light illumination Motorized*** - - - - - O - + - +
Manual O O O O O O + O + O
Reflected-light luminous-field
diaphragm Motorized - - - - - O - O - O

INSTRUMENT DECRIPTION
Carl Zeiss Equipment and compatibility table Axio Imager
18 430000-7344-001 M70-2-0020 e 06/2009
Type of Stand
Component Option A2
LED A2 M2p M2 D2 Z2 A2 m
M2
m
D2
m Z2m
Manual O O O O O O O O O O
Reflected-light aperture
diaphragm Motorized - - - - - O - O - O
Manual O O O O O O O O O O
Reflected-light two-position
filter wheel Motorized - - - O - O - O - O
Manual O O O O O O O O O O
FL attenuator Motorized - - - - - O - O - O
Manual + + - - + - + - + -
Reflected light / transmitted
light selection Software - - + + - + - + - +
Manual + + - - + - + - + -Mixed light
with extra power supply Software - - + + - + - + - +
Manual + + - - + - + - + -
Motorized, 25 nm - - + + - - - + - -
Focus (z-axis)
High-performance focus
(motorized, 10 nm) - - - - - + - - - +
TFT display - - - O + - + - + - +
Apotome - O O O O O O O O O O
External - - + + - + - + - +
Power supply Internal + + - - + - + - + -
Mechanical CAN stages Motorized**** O O O O O O O O O O
Piezo O O O O O O O O O O
Scanning stages DC / stepper motors O O O O O O O O O O
With manual stage O O O O O O O O O O
Fast z-piezo operation With scanning stage O O O O O O O O O O
Motorized 2TV tube - - - O O - O - O - O
Manual O O O O O O O O O O
Condensers Motorized - - O O - O - O - O
+ = Included in stand
O = Optionally available
O* = Included only, if equipped with Light Control Mot. 430000-9304-000
- = Not possible
* = Motorized (6-position and 10-position) reflector turret may be used.
** = ACR function not available with Axio Imager D2 and D2m.
*** = All reflected-light illumination systems come with a motorized shutter.
For fluorescence applications, it may be optionally replaced by a high-speed shutter.
**** = USB/CAN converter 432909 is required for use on Axio Imager A2 LED, A2, A2m, D2 and D2m.
***** = Motorized only if used with an objective nosepiece.

INSTRUMENT DECRIPTION
Axio Imager System overview of Axio Imager Carl Zeiss
M70-2-0020 e 06/2009 430000-7344-001 19
2.4 System overview of Axio Imager

INSTRUMENT DECRIPTION
Carl Zeiss System overview of Axio Imager Axio Imager
20 430000-7344-001 M70-2-0020 e 06/2009