Zeiss Standard 25 ICS User manual

Standard 25 ICS
Transmitted Light
Microscope
Operating Manual

Standard 25 ICS
II B 40-029 e 01/98
Knowledge of this manual is required for the operation of the instrument. Would you therefore please
make yourself familiar with the contents of this manual and pay special attention to hints concerning the
safe operation of the instrument.
The specifications are subject to change; the manual is not covered by an update service.
© Unless expressly authorized, forwarding and duplication of this document, and the utilization and
communication of its contents are not permitted. Violations will entail an obligation to pay
compensation.
All rights reserved in the event of granting of patents or registration of a utility model.
Issued by: Carl Zeiss
Mikroskopie
D-07740 Jena
Telephone: (**49) 03641 / 64-1616
Telefax: (**49) 03641 / 64-3144
Internet: [email protected]
http://www.zeiss.de
Number of this manual: B 40-029 e
Date of issue: 01/98

Standard 25 ICS
B 40-029 e 01/98 III
CONTENTS
Page
INTRODUCTION........................................................................................................................I
Title page...................................................................................................................................I
Copyright.................................................................................................................................. II
Contents...................................................................................................................................III
Notes on instrument safety.......................................................................................................IV
Warranty notes ........................................................................................................................VI
Overall view ............................................................................................................................ VII
1 DESCRIPTION........................................................................................................................1-3
1.1 Name and intended application ..............................................................................................1-3
1.2 Instrument description and main features................................................................................1-3
1.3 Microscope configurations and modules .................................................................................1-4
1.4 Objectives ..............................................................................................................................1-6
1.5 Eyepieces ...............................................................................................................................1-7
1.6 Stage micrometers and eyepiece reticles .................................................................................1-8
1.7 Technical Data......................................................................................................................1-10
2 START-UP .............................................................................................................................2-3
2.1 Unpacking the instrument ......................................................................................................2-3
2.2 Attachment of binocular tube 45°/20 ICS or binocular phototube 35°/20 ICS...........................2-3
2.3 Screwing-in of objectives........................................................................................................2-3
2. Insertion of eyepieces .............................................................................................................2-4
2.4.1 Insertion of eyepiece reticle.....................................................................................................2-4
2.4.2 Compensation of ametropia when eyepiece reticles are used ..................................................2-5
2.5 Setting of interpupillary distance and viewing height...............................................................2-5
2.6 Attachment of specimen holder to mechanical stage 75x30 R .................................................2-6
2.7 Attachment of condensers......................................................................................................2-7
2.7.1 Attach condenser 0.9 Z ..........................................................................................................2-7
2.7.2 Attach condenser II Z 0.9 Ph1, 2, 3 and D (0.6/0.9) .................................................................2-7
2.8 Connecting the instrument to the line.....................................................................................2-8

Standard 25 ICS
IV B 40-029 e 01/98
3 OPERATION..........................................................................................................................3-3
3.1 Switch on the instrument.......................................................................................................3-3
3.2 Setting of transmitted-light brightfield for KÖHLER illumination...............................................3-4
3.3 Setting of transmitted-light phase contrast .............................................................................3-6
3.4 Setting of transmitted-light darkfield ......................................................................................3-8
3.5 Setting of transmitted light polarization contrast.....................................................................3-9
3.6 Measurement of lengths ......................................................................................................3-10
3.7 Attachment of photomicrography equipment....................................................................... 3-11
3.7.1 Attachment of SLR camera, e.g. CONTAX 167 MT................................................................ 3-11
3.7.2 Attachment of MC 80 DX microscope camera (35 mm film cassette)..................................... 3-13
3.8 Attachment of adapters for video cameras............................................................................3-14
3.9 Insertion of 8x drawing eyepiece ..........................................................................................3-16
4 CARE, MAINTENANCE AND TROUBLESHOOTING............................................................... 4-3
4.1 Care and maintenance of the instrument................................................................................4-3
4.2 Troubleshooting.....................................................................................................................4-4
4.2.1 Changing the fuses................................................................................................................4-4
4.2.2 Changing the 6 V 20 W halogen lamp....................................................................................4-5
4.2.3 Troubleshooting table ........................................................................................................... 4-6
4.3 Table of spares, wearing parts and tools .............................................................................. 4-10
4.4 Requesting service................................................................................................................ 4-11
Annex...................................................................................................................................A-1
List of abbreviations ...............................................................................................................A-3
Physical and technical units.....................................................................................................A-4
Certification in accordance with DIN EN ISO 9001 / DIN EN 46001...........................................A-5
EC conformity declaration......................................................................................................A-7

Standard 25 ICS
B 40-029 e 01/98 V
Notes on instrument safety
The Standard 25 ICS microscope was designed, produced and tested in compliance with DIN 61010-1
(IEC 1010-1), Safety requirements for electrical measuring, control and laboratory instruments, and
meets the requirements of appendix I of directive 73/23/EC and the relevant CSA and UL directives. The
microscope meets the requirements of the EC directive 89/336/EC and the EMC legislation of November
9, 1992. This operation manual includes information and warnings which must be observed by the user.
The following warning and information symbols are used in this manual:
NOTE
This symbol (hand) is a warning which you must observe under all circumstances.
CAUTION
This symbol (warning triangle) is a warning which indicates a hazard to the operation of the
instrument.
CAUTION
Disconnect the instrument from the line!

Standard 25 ICS
VI B 40-029 e 01/98
The Standard 25 ICS microscope, including its original accessories, may only be used for the microscope
techniques described in this manual.
Particular attention must be paid to the following warning notes:
The Standard 25 ICS microscope is categorized as Protection Class I and has been allocated the
protection degree IP 20. The power plug must be inserted in a properly installed socket
featuring a grounding contact. The grounding effect must not be made ineffective by an
extension cable which does not have a protective ground wire.
Before switching on the instrument, check whether it is suitable for the line voltage present.
Admissible voltage: 100...240 V AC, 50...60 Hz (depending on the model).
Always disconnect the instrument from the line before opening the instrument, before
changing the voltage and before changing the fuses.
When changing the instrument fuses, make sure to use only those of the rated power required
and the type indicated. The use of makeshift fuses and the short-circuiting of the fuse holders
are not permitted.
If it is established that the protection measures are no longer effective, the instrument must be
switched off and safeguarded against inadvertent operation.
Dust and dirt can impair the performance of the instrument. Therefore, protect the instrument
against these influences as far as possible. If the microscope will not be used for longer
periods of time, it must be protected using the dust cover.
The instrument must be operated by trained personnel only who must be aware of the
possible danger involved with microscopy and the relevant application.

Standard 25 ICS
B 40-029 e 01/98 VII
The Standard 25 ICS microscope is an optical precision instrument which can be impaired in its
performance or damaged when handled improperly.
Notes on warranty:
The manufacturer guarantees that the instrument has no material and production defects
when delivered. You must inform us of any defects immediately and we must do anything to
minimize the damage. If the manufacturer is informed of such a defect, he is obliged to
remove it; it is his decision whether he does this by repairing the instrument or by delivering an
instrument free of any defect. No guarantee is provided for defects caused by natural wear
(wearing parts in particular).
The instrument manufacturer is not liable for damage caused by faulty operation, negligence
or any other meddling with the instrument, or the use of accessories from other
manufacturers. This forfeits all the claims against warranty.
With the exception of the work specified in this manual, no maintenance or repair of the
Standard 25 ICS may be performed. Repairs may only be performed by Zeiss service staff or
specially authorized personnel. Should any defect occur with the instrument, please get in
touch with your local Zeiss agency first.

Standard 25 ICS
VIII B 40-029 e 01/98
Overall view of the Standard 25 ICS microscope with polarization equipment

Standard 25 ICS
B 40-029 e 01/98 1-1
DESCRIPTION
Contents
1 DESCRIPTION.................................................................................................................1-3
1.1 Name and intended application........................................................................................1-3
1.2 Instrument description and main features.........................................................................1-3
1.3 Microscope configurations and modules...........................................................................1-4
1.4 Objectives........................................................................................................................1-6
1.5 Eyepieces.........................................................................................................................1-8
1.6 Stage micrometers and eyepiece reticles...........................................................................1-8
1.7 Technical Data...............................................................................................................1-10
List of illustrations
Fig. 1-1 Microscope modules........................................................................................................1-2
Fig. 1-2 Standard 25 ICS microscope configurations.......................................................................1-4

Standard 25 ICS
1-2 B 40-029 e 01/98
Fig. 1-1 Microscope modules
1 Eyepieces 5 Condenser carrier with condenser
2 Binocular tube 6 Mechanical stage with specimen holder
3 Stand 7 Objective
4 Coaxial coarse and fine drive 8 Objective nosepiece