Zeiss Axiovert 100 User manual

3D Microscopy for
Axiovert 100/135/135 M
Operating Manual

Copyright 3D Microscopy
2B 40-022-4 e 07/98
Knowledge of this manual is required for the operation of the instrument. Would you therefore
please make yourself familiar with the contents of this manual and pay special attention to hints
concerning the safe operation of the instrument.
The specifications are subject to change.

Unless expressly authorized, forwarding and duplication of this document, and the utilization and
communication of its contents are not permitted. Violations will entail an obligation to pay
compensation.
All rights reserved in the event of granting of patents or registration of a utility model.
Issued by: Carl Zeiss
Mikroskopie
D-07740 Jena
Telephone: [phone]
Telefax: (03641) 64-31 44
Internet: mikro @ zeiss.de
http://www.zeiss.de
Number of this manual: B 40-022-4 e
Date of issue: 07/98

3D Microscopy Contents
B 40-022-4 e 07/98 3
Contents
1 Notes on safety ................................................................................................... 4
1.1 General.............................................................................................................................. 4
1.2 Regulations concerning instrument safety and electromagnetic compatibility (EMC)........... 4
1.3 Notes on unpacking, transport and storage........................................................................ 5
1.4 Hints on use....................................................................................................................... 5
1.5 Notes on maintenance....................................................................................................... 5
2 General................................................................................................................. 6
2.1 Application ....................................................................................................................... VI
2.2 Function............................................................................................................................. 7
2.2.1 Eyepiece version................................................................................................................. 7
2.2.2 Monitor version.................................................................................................................. 8
2.2.3 Combination of eyepiece and monitor version ................................................................ 10
2.3 Maintenance and care ..................................................................................................... 11
2.3.1 General............................................................................................................................ 11
2.3.2 Cleaning of optical components....................................................................................... 11
2.3.3 Change of voltage and fuses............................................................................................ 11
2.3.4 Troubleshooting............................................................................................................... 12
3 Technical Data ................................................................................................... 13
Certification in accordance with DIN EN ISO 9001 and DIN EN 46001
EC Declaration of Conformity
Annex (optional)
3D microscopy for Axiotron 2
3D microscopy for Axiotech 100
3D microscopy for Axiotech 100 vario
3D microscopy for Axiovert 100/135/135 M
3D microscopy for Axiovert 25 CA
3D microscopy for Axioplan 2/Axiophot 2
3D microscopy for Axioskop
3D microscopy for Axioskop 2

Notes on safety 3D Microscopy
4B 40-022-4 e 07/98
1 Notes on safety
1.1 General
Please make yourself familiar with the contents
of these operating instructions before
attaching and starting up the components for
3D microscopy. Additional information is
available from our maintenance service or from
authorized agencies.
For the function of 3D microscopy in
combination with the

Axiotron 2

Axiotech 100

Axiotech 100 vario

Axiovert 100/135/135 M

Axiovert 25 CA

Axioplan 2/Axiophot 2

Axioskop

Axioskop 2
please see the operating instructions of these
microscopes.
When using video components from KAPPA,
please comply with the supplied manual "Color
TV camera for microscopy and 3D images".
Special regard must be paid to the notes on
instrument safety included in this manual.
To guarantee the safe operation and function
of the components for 3D microscopy, it is
necessary under any circumstances to take the
precaution measures and observe the warnings
contained in the operating instructions.
The following symbols are used for the
warnings:
Caution!
Non-observance of the safety notes constitutes
a hazard for the user.
Caution!
Non-observance of the safety notes constitutes
a hazard for the instrument.
Caution!
Disconnect the instrument from the line before
opening it.
☞Note!
Notes which must be observed.
1.2 Regulations concerning
instrument safety and
electromagnetic
compatibility (EMC)
The components for 3D microscopy have been
designed and tested in compliance with EN
61010, Part 1 (VDE 0411). From the safety
viewpoint, they left the factory in a perfect
state.
They meet the requirements of the EC directive
89/336/EC and the EMC legislation of
November 9th 1992 and the legal regulations
for accident prevention.
The components for 3D microscopy meet the
EC requirements and are CE-labelled.

3D Microscopy Notes on unpacking, transport and storage
B 40-022-4 e 07/98 5
1.3 Notes on unpacking,
transport and storage
The components for 3D microscopy must be
transported in their original packaging. It is
recommended to use the original packaging
also for storage.
The transport and storage temperatures must
comply with those listed under "Technical
Data".
When unpacking the components which are
equipped with polarizing foil (3D illumination
shutter, eyepieces with 3D shutter), make sure
to be particularly careful, since the foil can be
easily scratched.
1.4 Hints on use

The components for 3D microscopy can
only be used with halogen lamps up to
max. 100 W.

The power plug must be inserted in a
socket featuring a grounding (earth)
contact.
The grounding effect must not be reduced
by an extension cable which does not have
a protective ground wire.

Prior to connection to the line, check
whether the voltage set on the selector at
the rear of the 3D-CONTROL DAS-3 unit
complies with the line voltage; it may be
necessary to change from 230 V to 115 V.
The change to 115 is only required for the
3D-CONTROL DSA-3 unit.
For further details, see "Care and
Maintenance" on page VIII.

Cable connections may only be made if the
various components are switched off.

For the video components from KAPPA, it is
absolutely necessary to use only the
connectors listed in the manual "Color TV
camera for microscopy and 3D images".

Make sure that the cables are not bent or
laid out in too narrow a radius.

The optical components must be protected
from dirt and mechanical damage;
special care must be taken to prevent the
polarizing foils of the 3D illumination
shutter from being touched.

The components for 3D microscopy must be
protected from impact and humidity.
1.5 Notes on maintenance

To enable 3D microscopy to function
perfectly, it is necessary to use only
components and spares marketed or
admitted by us. When in doubt, please
contact our service staff.

With the exception of the work described in
the "Care and maintenance" chapter, the
user must not perform any repairs or
changes himself. We would like to
emphasize that all other changes and
repairs must be performed by specially
authorized personnel.

The manufacturer is not liable for damage
caused by unauthorized operation. This
forfeits all the claims against warranty.

General
Application 3D Microscopy
6B 40-022-4 e 07/98
2 General
2.1 Application
3D microscopy permits the stereoscopic viewing
of microscope specimens and objects

up to highest magnifications

using all objectives

eyepiece and monitor observation

in reflected and transmitted light

on upright and inverted microscopes

in brightfield and phase contrast

in real color

in real time

with extended depth of focus

with improved resolution

storage via recorder or image memory of the
PC

documentation via video prints or color
photos
The following microscopes can be upgraded
with the components for 3D microscopy:

Axiotron 2

Axiotech 100

Axiotech 100 vario

Axiovert 100/135/135 M

Axiovert 25 CA

Axioplan 2/Axiophot 2
3D microscopy is ideal for the following
applications in biomedicine:

micromanipulation

patch-clamp technique

in-vitro fertilization

examination of endocytosis and xenobiotics

examination of phagocytosis

injection of nucleotides

examination of nerve cells
3D microscopy is ideal for the following
applications in materials microscopy:

defect analysis of semiconductor
components

observation of surface topography in
microtechniques

materials examinations in construction
engineering

rupture analysis

examination of the structure of microfibers

examination of the structure of crystalline
materials

examination of compound materials.

Axioskop

Axioskop 2

3D Microscopy Function

Eypiece version
B 40-022-4 e 07/98 7
2.2 Function
The dynamic technique on which 3D
microscopy is based can be used with any light
microscope with an accessible aperture
diaphragm.
The technique can be used with upright or
inverted reflected-light and transmitted-light
microscopes.
A patent has been applied for the technique.
1 Eyepiece with 3D-Shutter
2 3D-CONTROL DSA-3
3 3D illuminator shutter
4 Objective
5 Object plane
Eyepiece version
in a reflected-light microscope
There are three different versions of 3D
microscopy:

eyepiece version

monitor version

combination of eyepiece and monitor
version
2.2.1 Eyepiece version
An LCD shutter ("3D illumination shutter") is
positioned close to the aperture diaphragm and
is used to open or close one half of the beam
path.
With the eyepiece version, the LCD shutter is
controlled via the "3D-CONTROL DSA-3" unit.
The centroid of the illumination beam path is
switched in such a way that the object is
illuminated alternately from a right and a left
stereo angle.
The eyepiece shutters are controlled at the
same pulse rate, i.e. each eye only sees the
image on its own side.
right eye

right image
left eye

left image
A flicker-free display of the stereo image
composed of the right and left images is made
possible by an appropriately high pulse rate.
When the illuminator - eye allocation is
changed, a pseudostereo effect is created
(height-changed image).
Resolution, contrast and depth can be
optimized with relation to the object via the
aperture diaphragm.

Function

Monitor version 3D Microscopy
8B 40-022-4 e 07/98
1 Eyepiece with 3D-Shutter
2 3D-CONTROL DSA-3
3 Depolarizer
4 3D illumination shutter
5 Condenser
6 Object plane
7 Objective
Eyepiece version
in a transmitted-light microscope
The depolarizer is required for minimization of
disturbances by the polarization equipment and
facilitates work with birefringent objects
2.2.2 Monitor version
An LCD shutter ("3D illumination shutter") is
positioned close to the aperture diaphragm and
is used to open or close half a side of the beam
path.
With the monitor version, the LCD shutter is
controlled via the "3MCU II" unit.
The centroid of the illumination beam path is
switched in such a way that the object is
illuminated alternately from a right and a left
stereo angle.
The camera and the monitor shutters are
controlled at the same pulse rate, i.e. each eye
only sees the image on its own side.
right eye

right image
left eye

left image
In the monitor version, allocation is made via
the display of the half images in two
perpendicular polarization directions which can
be allocated to the eyes by polarizing glasses.
A flicker-free display of the stereo image
composed of the right and left images is made
possible by an appropriately high pulse rate.
When the illuminator - eye allocation is
changed, a pseudostereo effect is created
(height-changed images).
Resolution, contrast and depth can be
optimized with relation to the object via the
aperture diaphragm.

3D Microscopy Function

Monitor version
B 40-022-4 e 07/98 9
1 Monitor shutter
2 Glasses
3 Control unit for monitor shutter
4 MCU II image memory
5 3D illumination shutter
6 Objective
7 Object plane
8 Camera
Monitor version
in a reflected-light microscope
1 Monitor shutter
2 Polarizing glasses
3 Camera
4 Objective
5 Object plane
6 Condenser
7 3D illumination shutter
8 MCU II image memory
9 Control unit for monitor shutter
Monitor version
in a transmitted-light microscope

Function

Combined version 3D Microscopy
10 B 40-022-4 e 07/98
1 Monitor shutter
2 Polarizing glasses
3 Camera
4 Control unit for monitor shutter
5 MCU II image memory
6 3D-CONTROL DSA-3
7 3D illumination shutter
8 Depolarizer
9 Object plane
10 Eyepiece with 3D Shutter
Combined version
in a transmitted-light microscope
2.2.3 Combination
of eyepiece and monitor version
An LCD shutter ("3D illumination shutter") is
positioned close to the aperture diaphragm and
is used to open or close one half of the beam
path.
With the combined version, the LCD shutter is
controlled via the "3D-CONTROL DSA-3" unit.
The centroid of the illumination beam path is
switched in such a way that the object is
illuminated alternately from a right and a left
stereo angle.
The eyepiece shutter and the camera and
monitor shutters are controlled at the same
pulse rate, i.e. each eye only sees the image
on its own side.
right eye

right image
left eye

left image
In the monitor version, allocation is made via
the display of the half images in two
perpendicular polarization directions which can
be allocated to the eyes by polarizing glasses.
A flicker-free display of the stereo image
composed of the right and left images is made
possible by an appropriately high pulse rate.
When the illuminator - eye allocation is
changed, a pseudostereo effect is created
(height-changed image).
Resolution, contrast and depth can be
optimized with relation to the object via the
aperture diaphragm.