Zeiss LSM 510 Inverted User manual

I
LSM 510
Laser Scanning Microscope
Operating Manual

INTRODUCTION LSM 510
II B 40-051 e 07/98
Knowledge of this manual is required for the operation of the instrument. Would you therefore please
make yourself familiar with the contents of this manual and pay special attention to hints concerning the
safe operation of the instrument.
The specifications are subject to change; the manual is not covered by an update service.
© Unless expressly authorized, forwarding and duplication of this document, and the utilization and
communication of its contents are not permitted. Violations will entail an obligation to pay
compensation.
All rights reserved in the event of granting of patents or registration of a utility model.
Issued by: Carl Zeiss
Mikroskopie
D-07740 Jena
Telephone: (**49) 03641 / 64-1616
Telefax: (**49) 03641 / 64-3144
Internet: [email protected]
http://www.zeiss.de
Number of this manual: B 40-051 e
Date of issue: 07/98

INTRODUCTION
LSM 510
B 40-051 e 07/98 III
Developed in Collaboration with the European Molecular Biology Laboratory (EMBL)
PF 102209
Meyerhofstr. 1
D-69012 Heidelberg
Phon: ++49-(0)-62221-387-0
Fax: ++49-(0)-62221-387-306

INTRODUCTION LSM 510
IV B 40-051 e 07/98

INTRODUCTION
LSM 510
B 40-051 e 07/98 V
How to make best use of the LSM 510 operating instructions
A few symbols in these operating instructions will help you to recognize the nature and purpose of
information immediately:
The WARNING symbol warns against hazards for the user that might arise when operating the
laser.
This WARNING symbol warns against hazards from dangerously high voltages.
The CAUTION symbol warns against faults and hazards that might arise during operation and
which might cause damage to the unit.
☞The NOTE symbol will help you to optimally solve your work problem. It represents a practical
tip which will help you to find out which settings and methods are capable of improving or
accelerating a procedure.
The HOT SURFACE symbol warns against hazards for the user that might arise when touching
the lamp housing during operation.
The MAINS PLUG symbol remembers service personal to pull the mains plug before opening
the device housing.
Depending on the problem, these operating instructions will supply you with various possibilities:
 If you want to know where to find certain general areas of information, refer to the following outline
of sections to get a general overview.
 You will find a detailed table of contents at the start of every chapter. There you will see at a glance
what topics are dealt with in detail.
Always remember:The time you invest in getting acquainted with the product will pay
for itself many times over in your application task.

INTRODUCTION LSM 510
VI B 40-051 e 07/98
Scope of Equipment Supplied
Country: ..............................................
Order number: ..............................................
Serial number: ..............................................
Delivery date: ..............................................
Custom configuration: ..............................................
Axioplan 2 MOT 000000-1028-778 
Axiovert 100 M SP 000000-1028-779 
Axiovert 100 M BP 000000-1028-780 
Objectives: ..............................................
..............................................
..............................................
Confocal Laser Scanning Module LSM 510
Configuration 1 000000-1027-076 
Configuration 2 000000-1027-077 
Configuration 3 000000-1027-078 
Configuration 4 000000-1027-079 
Configuration 5 000000-1027-080 
Configuration 6 000000-1027-081 
Configuration 7 000000-1027-082 
Configuration 8 000000-1027-083 
Configuration 9 000000-1027-084 
Configuration 10 000000-1027-085 
Configuration 11 000000-1027-086 
Configuration 12 000000-1027-087 
Configuration 13 000000-1027-088 
Configuration 14 000000-1031-725 
Configuration 15 000000-1031-726 
Configuration 16 000000-1031-727 
Control computer with 21“ monitor 000000-1032-142 
Control computer with two 21“ monitors 000000-1032-143 
The license to the LSM control software is included in each configuration 1...16.
Optional software;
LSM 510 basis software release 2.0 000000-1027-554 
LSM 510 evaluation software physiology 000000-1027-556 
LSM 510 software 3D for LSM 000000-1024-966 

INTRODUCTION
LSM 510
B 40-051 e 07/98 VII
Kodak XLS 8650 PS printer 417735 9003-000 
Transparency exposure device (D) 412410 9011-000 
Large system table 453031-0000-000 
Small sytem table 453032-0000-000 
System baseplate 453030-0101-000 
UV laser 412410 9015-000 
High resolution z-stage HRZ 200 for Axiovert 000000-1013-186 
High resolution z-stage HRZ 200 for Axioplan 2 000000-1013-187 
XY scanning stage for Axiovert 100 M BP 000000-1017-917 
XY scanning stage for Axioplan 2 MOT 000000-1027-823 
Filter changer 18 mm 453070-0000-000 
Set of INDO filters 447960-0000-000 
Set of SNARF filters 447961-0000-000 
The LSM 510 in the configuration as checked above
was installed and handed to the customer in functional condition
on ................................................
by ................................................
Phone: ................................................
Fax: ................................................
The customer has been instructed on how to operate and
maintain the equipment.
(Place)......................................................, (date) .....................................
...............................................................
Carl Zeiss Jena GmbH
Microscopy Division
...............................................................
Customer
One copy to be kept by customer
One copy to be kept by Carl Zeiss

INTRODUCTION LSM 510
VIII B 40-051 e 07/98
1This section contains general notes on device safety, safe operation, and possible hazards
caused by failure to observe the instructions.
2The Setup Requirements section outlines the installation and supply requirements of the
LSM 510 Microscope System, together with the relevant specifications.
3Here you will find an introduction to Laser Scanning Microscopy, with an explanation of the
principles of confocal imaging. The section also outlines the ways to present LSM image
series in three dimensions, and introduces you to the performance features of your
LSM 510.
4In the Operation section you will find the most important steps and procedures of the LSM
menu structure.The step-by-step description how to get an image will be shown by typical
application examples including the WINDOWS NT 4.0 graphic user environment.
5This section contains a description of the LSM 3D software package (basic program and
add-ons. At the same time, all functions and settings are presented in a systematic form
and in order in which they can be reached from the basic menu via sub-menus and dialog
boxes
.

INTRODUCTION
LSM 510
B 40-051 e 07/98 IX
1Notes on Device Safety
2LSM 510 Setup Requirements
3Introduction to Laser Scanning Microscopy
4Operation
5Software 3D Description

INTRODUCTION LSM 510
XB 40-051 e 07/98