Agilent Technologies 1290 Infinity II User manual

Agilent Technologies
Agilent 1290 Infinity II
Evaporative Light
Scattering Detector
User Manual

Agilent 1290 Infinity II ELSD User Manual
Notices
© Agilent Technologies, Inc. 2014
No part of this manual may be reproduced
in any form or by any means (including elec-
tronic storage and retrieval or translation
into a foreign language) without prior agree-
ment and written consent from Agilent
Technologies, Inc. as governed by United
States and international copyright laws.
Manual Part Number
G7102-90000
Edition
08/2014
Printed in Germany
Agilent Technologies
Hewlett-Packard-Strasse 8
76337 Waldbronn
This product may be used as a com-
ponent of an in vitro diagnostic sys-
tem if the system is registered with
the appropriate authorities and com-
plies with the relevant regulations.
Otherwise, it is intended only for gen-
eral laboratory use.
Warranty
The material contained in this docu-
ment is provided “as is,” and is sub-
ject to being changed, without notice,
in future editions. Further, to the max-
imum extent permitted by applicable
law, Agilent disclaims all warranties,
either express or implied, with regard
to this manual and any information
contained herein, including but not
limited to the implied warranties of
merchantability and fitness for a par-
ticular purpose. Agilent shall not be
liable for errors or for incidental or
consequential damages in connection
with the furnishing, use, or perfor-
mance of this document or of any
information contained herein. Should
Agilent and the user have a separate
written agreement with warranty
terms covering the material in this
document that conflict with these
terms, the warranty terms in the sep-
arate agreement shall control.
Technology Licenses
The hardware and/or software described in
this document are furnished under a license
and may be used or copied only in accor-
dance with the terms of such license.
Restricted Rights Legend
If software is for use in the performance of a
U.S. Government prime contract or subcon-
tract, Software is delivered and licensed as
“Commercial computer software” as
defined in DFAR 252.227-7014 (June 1995),
or as a “commercial item” as defined in FAR
2.101(a) or as “Restricted computer soft-
ware” as defined in FAR 52.227-19 (June
1987) or any equivalent agency regulation
or contract clause. Use, duplication or dis-
closure of Software is subject to Agilent
Technologies’ standard commercial license
terms, and non-DOD Departments and
Agencies of the U.S. Government will
receive no greater than Restricted Rights as
defined in FAR 52.227-19(c)(1-2) (June
1987). U.S. Government users will receive
no greater than Limited Rights as defined in
FAR 52.227-14 (June 1987) or DFAR
252.227-7015 (b)(2) (November 1995), as
applicable in any technical data.
Safety Notices
CAUTION
A CAUTION notice denotes a
hazard. It calls attention to an
operating procedure, practice, or
the like that, if not correctly per-
formed or adhered to, could
result in damage to the product
or loss of important data. Do not
proceed beyond a CAUTION
notice until the indicated condi-
tions are fully understood and
met.
WARNING
A WARNING notice denotes a
hazard. It calls attention to an
operating procedure, practice,
or the like that, if not correctly
performed or adhered to, could
result in personal injury or
death. Do not proceed beyond a
WARNING notice until the indi-
cated conditions are fully under-
stood and met.

Agilent 1290 Infinity II ELSD User Manual 3
In This Book
In This Book
This manual contains information on:
•Agilent 1290 Infinity II ELSD (G7102A)
1 Introduction to the Detector
This chapter gives an introduction to the module, instrument overview and
internal connectors.
2 Site Requirements and Specifications
This chapter provides information on environmental requirements, physical
and performance specifications.
3 Installing the Module
This chapter gives information about the installation of your ELSD.
4 LAN Configuration
This chapter provides information on connecting the module to the control
software.
5 Using the Module
This chapter explains the operational parameters of the ELSD.
6 Optimizing Performance
This chapter gives hints on how to optimize the performance or use
additional devices.
7 Troubleshooting and Diagnostics
This chapter gives an overview about the troubleshooting and diagnostic
features.

4 Agilent 1290 Infinity II ELSD User Manual
In This Book
8 Error Information
This chapter describes the meaning of error messages, and provides
information on probable causes and suggested actions on how to recover
from error conditions.
9 Maintenance
This chapter describes the maintenance of the ELSD.
10 Parts and Materials for Maintenance
This chapter provides information on parts for maintenance.
11 Appendix
This chapter provides addition information on safety, legal and web.

Agilent 1290 Infinity II ELSD User Manual 5
Contents
Contents
1 Introduction to the Detector 7
Introduction to the ELSD 8
System Overview 9
2 Site Requirements and Specifications 17
Pre-installation Requirements 18
Site Requirements 19
Physical Specifications 22
Performance Specifications 23
3 Installing the Module 25
Unpacking the Module 26
Installing the Module 28
4 LAN Configuration 33
What You Have to Do First 34
TCP/IP Parameter Configuration 35
PC and Agilent OpenLAB ChemStation and EZChrom Setup 43
5 Using the Module 49
Before Using the Detector 50
Instrument Controls 51
Operational Parameters 55
Controlling the ELSD During an Injection 59
Controlling the ELSD within ChemStation or EZChrom 62
General Considerations 63

6 Agilent 1290 Infinity II ELSD User Manual
Contents
6 Optimizing Performance 67
Do’s and Don’ts of ELS Detection 68
Location of the Detector Module 69
Pumping systems 70
Mobile phase priming 71
7 Troubleshooting and Diagnostics 73
Troubleshooting 74
Troubleshooting an HPLC System 75
General Problems 76
8 Error Information 83
What Are Error Messages 84
Module Specific Error Messages 85
9 Maintenance 95
Introduction to Maintenance 96
Cautions and Warnings 97
Cleaning the Module 98
Inspection of Cables 99
Drying the Diffuser 100
Cleaning the Nebuliser 101
Cleaning Evaporator Tube 102
Putting the Instrument into Storage 103
Updating Detector Firmware 104
10 Parts and Materials for Maintenance 109
Identifying Parts and Materials 110
11 Appendix 111
General Safety Information 112
Waste Electrical and Electronic Equipment Directive 115
Radio Interference 116
Agilent Technologies on Internet 117

7
Agilent 1290 Infinity II ELSD User Manual
Agilent Technologies
1
Introduction to the Detector
Introduction to the ELSD 8
System Overview 9
Basic Principles of Operation 9
Operational Parameters 12
Overview of ELS Detector 13
This chapter gives an introduction to the module, instrument overview and
internal connectors.

8 Agilent 1290 Infinity II ELSD User Manual
1Introduction to the Detector
Introduction to the ELSD
Introduction to the ELSD
The Evaporative Light Scattering Detector is a unique and highly sensitive
detector for semi- volatile and non- volatile solutes in a liquid stream. It
is mainly used as a concentration detector for High Performance Liquid
Chromatography (HPLC). The solvent stream containing the solute material
is nebulized and carried by a gas flow through an evaporation chamber.
The solvent is volatilized, leaving a mist of solute particles that scatter
light to a photosensitive device. The signal is amplified and a voltage
output provides the concentration of the solute particles passing through
the light.
The Agilent 1290 Infinity II ELSD may be used alone, or as one of several
detectors in a GPC or HPLC system. As the solvent or eluent is
evaporated in the course of the analysis, the Agilent 1290 Infinity II ELSD
must be the last in series if used in conjunction with other detectors. If
the Agilent 1290 Infinity II ELSD is being used as the last detector in a
series, care must be taken not to exceed the recommended backpressure
in detector cells in other units.
This manual instructs the user in the installation and operation of the
Agilent 1290 Infinity II ELSD for stand- alone use and control using
Agilent OpenLAB (ChemStation and EZChrom editions) and Agilent
ChemStation.

Agilent 1290 Infinity II ELSD User Manual 9
Introduction to the Detector 1
System Overview
System Overview
Basic Principles of Operation
Nebulization
The eluent inlet is connected to the nebuliser via a short length of
stainless steel capillary tube. The incoming eluent stream passes through
the heated nebuliser and is mixed with the incoming nebuliser gas stream.
The mixed gas and eluent stream form an aerosol plume containing a
uniform dispersion of droplets that then passes as a continuous flow into
the evaporator section. Any larger droplets or the inefficiently nebulized
droplets collect in the nebuliser chamber waste trap and then drain off via
the waste outlet into a collection bottle.
Evaporation
After nebulization the atomized spray is propelled through the evaporation
tube assisted by the carrier gas. In the evaporator section the solvent is
removed leaving a stream of dry particles of the analyte. A diffuser
located in the evaporator assists in the drying of the particles, acting as
an efficient heat exchanger, prevents ballistic particles reaching the
scattering chamber and randomizes the particle plume. The ELS Detector
uses patented gas flow technology in the evaporation zone to aid
evaporation at low temperatures. By adding a stream of dry nitrogen
(evaporation gas) at the entrance of the evaporator tube less volatile
solvents (for example, water) are easily evaporated. This evaporation gas
is controlled by the user and facilitates sub- ambient operation.

10 Agilent 1290 Infinity II ELSD User Manual
1Introduction to the Detector
System Overview
Detection
Light in the optical chamber is passed through the instrument at right
angles to the direction of particle flow. A light trap is located opposite the
source of light to capture the transmitted incident beam eliminating
internal reflections within the instrument body. When pure solvent is
being evaporated, only its vapor passes through the light path and the
amount of light scattered to the photomultiplier is small and gives a
constant baseline response. When a non- volatile solute is present a
particle cloud passes through the light path, causing light to be scattered.
This scattered light enters the optical aperture of the detection system and
generates a signal response from the photodiode in real time. The quantity
of light detected is dependent on the solute concentration and solute
particle size distribution.
Figure 1 Principles of Operation
&#CZWja^oVi^dc '#:kVedgVi^dc (#9ZiZXi^dc