Agilent Technologies Cary 60 User manual

Agilent Cary 60 Spectrophotometer
User’s Guide

2 Agilent Cary 60 Spectrophotometer User’s Guide
Notices
Manual Part Number
G6860-90001
Edition 5, March 2019
Copyright
© Agilent Technologies, Inc. 2011,
2014-2016, 2019
No part of this manual may be
reproduced in any form or by any
means (including electronic storage
and retrieval or translation into a
foreign language) without prior
agreement and written consent from
Agilent Technologies, Inc. as governed
by United States and international
copyright laws.
Agilent Technologies Australia (M)
Pty Ltd
679 Springvale Road
Mulgrave, Victoria 3170 Australia
www.agilent.com
Instrument Manufacturing
Manufactured by Agilent Technologies
Bayan Lepas Free Industrial Zone,
Penang, PG, 11900, MY
Printed in USA
Warranty
The material contained in this
document is provided “as is,” and is
subject to being changed, without
notice, in future editions. Further, to the
maximum extent permitted by
applicable law, Agilent disclaims all
warranties, either express or implied,
with regard to this manual and any
information contained herein, including
but not limited to the implied
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fitness for a particular purpose. Agilent
shall not be liable for errors or for
incidental or consequential damages in
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Should Agilent and the user have a
separate written agreement with
warranty terms covering the material in
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terms, the warranty terms in the
separate agreement shall control.
Technology Licenses
The hardware and/or software
described in this document are
furnished under a license and may be
used or copied only in accordance with
the terms of such license.
Restricted Rights Legend
If software is for use in the
performance of a U.S. Government
prime contract or subcontract,
Software is delivered and licensed as
“Commercial computer software” as
defined in DFAR 252.227-7014 (June
1995), or as a “commercial item” as
defined in FAR 2.101(a) or as
“Restricted computer software” as
defined in FAR 52.227-19 (June 1987)
or any equivalent agency regulation or
contract clause. Use, duplication or
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commercial license terms, and non-
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U.S. Government will receive no
greater than Restricted Rights as
defined in FAR 52.227-19(c)(1-2) (June
1987). U.S. Government users will
receive no greater than Limited Rights
as defined in FAR 52.227-14 (June
1987) or DFAR 252.227-7015 (b)(2)
(November 1995), as applicable in any
technical data.
Safety Notices
A CAUTION notice denotes a hazard. It
calls attention to an operating
procedure, practice, or the like that, if
not correctly performed or adhered to,
could result in damage to the product
or loss of important data. Do not
proceed beyond a CAUTION notice
until the indicated conditions are fully
understood and met.
A WARNING notice denotes a hazard.
It calls attention to an operating
procedure, practice, or the like that, if
not correctly performed or adhered to,
could result in personal injury or
death. Do not proceed beyond a
WARNING notice until the indicated
conditions are fully understood and
met.
CAUTION
WARNING

Contents
Agilent Cary 60 Spectrophotometer User’s Guide 3
Contents
1General Information 7
User Documentation 7
Conventions 8
Notes and Tips 8
Verifying Safe State 8
Lamp Module 9
Modules, Covers and Panels 9
Other Precautions 9
Warning and Other Symbols 10
CE Compliance 11
Electromagnetic Compatibility 12
ICES/NMB-001 13
South Korean Class A EMC declaration 13
2Specifications 15
Measurement Category 15
Pollution Degree 15
Environmental Conditions 15
Weights and Dimensions 16
Electrical Specifications 17
Mains Supply 17
Computer Requirements 18
3Installation 19
Installation 19

Contents
4 Agilent Cary 60 Spectrophotometer User’s Guide
Unpacking 20
Installing the Cary WinUV Software 21
Installing the Hardware 22
Registering and Validating Your Cary 60 22
Running the Tutorials 23
Cell Holder 24
Installation 24
Alignment 25
Other Sample Holders 26
Installing Accessories 26
4Introduction 27
Instrument Overview 27
Indicator Lamp 27
Connections 28
Using the Software 28
5Troubleshooting 29
Access Denied 29
Problem 29
Solution 29
No Green Light Indicating that the Cary 60 is Powered 30
Problem 30
Solution 30
Start Button Replaced with Connect Button 30
Problem 1 30
Solution 31
Problem 2 31

Contents
Agilent Cary 60 Spectrophotometer User’s Guide 5
Solution 31
Absorbance is 10 Abs and Fluctuates Wildly During a Scan 32
Problem 32
Solution 32
Instrument Performance Testing 33
Problem 33
Solution 33
Wavelength Calibration 33
Problem 33
Solution 34
Front Panel LED Troubleshooting 34
LED Won’t Light Up 34
Solid Red LED 34
Flashing Red LED 34
6Maintenance and Spare Parts 35
Maintenance 35
Spare Parts 35

Contents
6 Agilent Cary 60 Spectrophotometer User’s Guide
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General Information
Agilent Cary 60 Spectrophotometer User’s Guide 7
1General Information
User Documentation 7
Lamp Module 9
Modules, Covers and Panels 9
Other Precautions 9
Warning and Other Symbols 10
CE Compliance 11
Your Agilent instrument and accessories have been carefully designed so that
when used properly you have an accurate, fast, flexible and safe analytical system.
Information about safety practices appears throughout the documentation (both
hard copy and online) provided with your instrument and accessories to help you
safely operate the instrument and accessories. Before using the instrument or
accessories, you must thoroughly read these safety practices. ALWAYS operate
the instrument and accessories in accordance with these safety practices.
User Documentation
You have been provided with the following documentation to help you set up and
operate your Agilent Cary 60 spectrophotometer:
•Cary 60 Installation Card, with information on unpacking the instrument and
setting up the system.
•This manual, with safety practices and hazards information, instructions for
installing and maintaining the components of the Cary 60, and troubleshooting
information.
•Cary Tutorials which provide step-by-step instructions for installing selected
accessories and programming applications.
•Extensive Help (in English only) provided with the Cary WinUV software
containing context-sensitive Help, step-by-step instructions for frequently
performed analyses and instructions for using any accessories you ordered.

General Information
8 Agilent Cary 60 Spectrophotometer User’s Guide
Conventions
The following conventions have been used throughout the documentation:
•Menus, menu items, buttons and check boxes have been typed in bold. For
example, ‘click OK’ and ‘From the Edit menu, choose Copy’.
•ALL CAPITALS indicate keyboard commands. For example, ‘Press ENTER’ and
‘Press SHIFT+F3’.
NOTE Throughout this manual, UV Dissolution and UV Fiber Optic Dissolution users
should replace “Cary WinUV Software” with “UV Dissolution Software” or “UV Fiber
Optic Dissolution Software” respectively.
Notes and Tips
A Note is used to give advice or information.
A Tip is used to give practical hints to help you achieve the best possible
performance from your instrument.
Verifying Safe State
The following general safety precautions must be observed during all phases of
operation, maintenance and service of this instrument.
To ensure continued safety of the instrument after maintenance or service
procedures verify the instrument is returned to a safe state for the user. This
includes running performance checks to verify the instruments safety systems are
functioning correctly. Check the general condition of the instrument during
operation for wear or signs of corrosion that are likely to inhibit function or safety.
Failure to comply with these precautions or with specific warnings elsewhere in
this manual violates safety standards of design, manufacture, and intended use of
the instrument. Agilent Technologies assumes no liability for the customer’s
failure to comply with these requirements.

General Information
Agilent Cary 60 Spectrophotometer User’s Guide 9
Lamp Module
The lamp is enclosed in a self-contained module. This module contains
components operating at high voltages. To avoid electric shock, NEVER
disassemble the module.
When operating, the lamp module emits high intensity light that can cause serious
damage to eyes. To avoid eye damage, never operate the lamp outside the
instrument.
Modules Covers and Panels
The Cary 60 spectrophotometer module that operators and other personnel will
routinely access is the sample compartment module.
The only other cover and panel that is customer accessible is the main cover and
lamp module and is to be removed ONLY when changing the lamp module and
aligning it. Consult the Cary WinUV Help for instructions and safety information.
Any other panels or covers that are retained by screws on the spectrophotometer
and accessories may be opened ONLY by Agilent service engineers.
Other Precautions
Do not block any ventilation grills present on the computer. Consult the manuals
supplied with your PC, monitor and printer/plotter for their specific ventilation
requirements.
Allow at least 50 mm (2 in.) of space on the sides, and 150 mm (6 in) at the rear of
the system to permit free air circulation.
Use of the Cary 60 system and accessories may involve materials, solvents and
solutions that are flammable, corrosive, toxic or otherwise hazardous.
Careless, improper, or unskilled use of such materials, solvents and solutions can
create explosion hazards, fire hazards, toxicity and other hazards which can result
in death, serious personal injury, and damage to equipment and property.

General Information
10 Agilent Cary 60 Spectrophotometer User’s Guide
ALWAYS ensure that laboratory safety practices governing the use, handling and
disposal of such materials are strictly observed. These safety practices should
include the wearing of appropriate safety clothing and safety glasses.
Warning and Other Symbols
The following is a list of symbols that appear in conjunction with warnings on the
spectrophotometer. The hazard they describe is also shown.
A triangular symbol indicates a warning. The meanings of the symbols that may
appear alongside warnings in the documentation or on the instrument itself are as
follows.
Electrical shock Eye hazard
Heavy weight
(danger to feet)
Heavy weight
(danger to hands)
The following symbol may be used on warning labels attached to the instrument.
When you see this symbol, refer to the relevant operation or service manual for the
correct procedure referred to by that warning label.

General Information
Agilent Cary 60 Spectrophotometer User’s Guide 11
The following symbols appear on the instrument for your information:
Indicates high voltage Xenon flash lamp operating under this
cover. To avoid eye damage NEVER look at the light either
directly or via a reflecting surface. ONLY Agilent trained and
authorized personnel may remove this cover.
Indique qu’une lampe Xénon flash haute tension fonctionne
sous ce couvercle. Pour éviter toutes lésions oculaires NE
JAMAIS regarder la lumière que ce soit directement ou par
l'intermédiaire d'une surface réfléchissante. SEUL le personnel
Agilent formé et autorisé peut retirer ce capot.
Indicates viewing hole to check the operation of the Xenon
flash lamp.
CE Compliance
Your Agilent Cary 60 spectrophotometer has been designed to comply with the
requirements of the Electromagnetic Compatibility (EMC) Directive and the Low
Voltage (electrical safety) Directive (commonly referred to as the LVD) of the
European Union. Agilent has confirmed that each product complies with the
relevant Directives by testing a prototype against the prescribed EN (European
Norm) standards.
Proof that a product complies with these directives is indicated by:
•the CE Marking appearing on the rear of the product, and
•the documentation package that accompanies the product containing a copy
of the Declaration of Conformity. The Declaration of Conformity is the legal
declaration by Agilent that the product complies with the directives listed
above and shows the EN standards to which the product was tested to
demonstrate compliance.

General Information
12 Agilent Cary 60 Spectrophotometer User’s Guide
Electromagnetic Compatibility
EN55011/CISPR11
Group 1 ISM equipment: group 1 contains all Industrial, Scientific and Medical
(ISM) equipment in which there is intentionally generated and/or used
conductively coupled radio- frequency energy which is necessary for the internal
functioning of the equipment itself.
Class A equipment is equipment suitable for use in all establishments other than
domestic and those directly connected to a low voltage power supply network
which supplies buildings used for domestic purposes.
This device complies with the requirements of CISPR11, Group 1, Class A as
radiation professional equipment. Therefore, there may be potential difficulties in
ensuring electromagnetic compatibility in other environments, due to conducted
as well as radiated disturbances.
Operation is subject to the following two conditions:
1This device may not cause harmful interference.
2This device must accept any interference received, including interference that
may cause undesired operation.
If this equipment does cause harmful interference to radio or television reception,
which can be determined by turning the equipment off and on, the user is
encouraged to try one or more of the following measures:
1Relocate the radio or antenna.
2Move the device away from the radio or television.
3Plug the device into a different electrical outlet, so that the device and the
radio or television are on separate electrical circuits.
4Make sure that all peripheral devices are also certified.
5Make sure that appropriate cables are used to connect the device to peripheral
equipment.
6Consult your equipment dealer, Agilent Technologies, or an experienced
technician for assistance.
Changes or modifications not expressly approved by Agilent Technologies could
void the user’s authority to operate the equipment.

General Information
Agilent Cary 60 Spectrophotometer User’s Guide 13
ICES/NMB-001
This ISM device complies with Canadian ICES-001.
Cet appareil ISM est conforme à la norme NMB-001 du Canada.
South Korean Class A EMC declaration
A 급 기기 ( 업무용 방송통신기자재 )
This equipment is Class A suitable for professional use and is for use in
electromagnetic environments outside of the home.
이 기기는 업무용 (A 급 ) 전자파적합기기로서 판 매자 또는 사용자는 이 점을 주
의하시기 바라 며 , 가정외의 지역에서 사용하는 것을 목적으 로 합니다 .

General Information
14 Agilent Cary 60 Spectrophotometer User’s Guide
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Specifications
Agilent Cary 60 Spectrophotometer User’s Guide 15
2Specifications
Measurement Category 15
Pollution Degree 15
Environmental Conditions 15
Weights and Dimensions 16
Electrical Specifications 17
Computer Requirements 18
Measurement Category
The Measurement category is IEC61010:I. Do not to use this equipment for
measurements within measurement categories II, III and IV.
Pollution Degree
The pollution degree is IEC61010:2. Pollution degree ‘2’ applies to a normal indoor
atmosphere.
Environmental Conditions
No draughts
No corrosive fumes
No vibration

Specifications
16 Agilent Cary 60 Spectrophotometer User’s Guide
NOTE For
optimum analytical performance
, it is recommended that the ambient
temperature of the laboratory be between
20 and 25 °C
and be held constant to
within ±2 °C throughout the entire working day.
The area should have a dust-free, low humidity atmosphere. Air conditioning is
recommended. The room should be temperature-controlled if your analyses are
particularly sensitive.
Condition
Altitude (m, ft)
Temp. (°C, °F)
Humidity (%RH)
non-condensing
Non-operating (transport)
0–4600, 0–15000
-40 to 70, -40 to158
<90
Operating within performance
specifications
0–3100, 0–10000
5–40, 41–104
15–80
Weights and Dimensions
Weight
Packed 23 kg (51 lb)
Unpacked 18 kg (40 lb)
Dimensions (width x depth x height)
Packed 595 x 710 x 350 mm (24 x 28 x 14 in)
Unpacked 477 x 567 x 196 mm (19 x 23 x 8 in)
The Cary 60 has been designed to withstand 10 kg, the approximate weight of a
17 in. LCD monitor.
The workbench should be about 90 cm (36 in) high. Remember to provide space
for the computer, monitor and printer.
To avoid damage through spillage of solutions and samples being analyzed, the
worktops should be covered with a material that is corrosion resistant and
impervious to liquids.
Allow at least two inches of space on both sides, and six inches at the rear of the
system to permit free air circulation. Power cord and all other connections are
located at the rear of the instrument. The Power switch is located on the front
panel.

Specifications
Agilent Cary 60 Spectrophotometer User’s Guide 17
Electrical Specifications
Mains Supply
A standard 3.2 A/12 V plug pack is provided. Power cords are provided based on
the user’s country requirements. Only the supplied power supply is to be used with
this equipment.
Table 1. System electrical specifications
System unit
Required supply voltage
Spectrophotometer
90-265 V AC
Frequency 47-63 Hz
The maximum power consumption is 60 VA, 38 W, 130 BTU/hr.
The installation of electrical power supplies must comply with the rules and/or
regulations imposed by local authorities responsible for the supply of electrical
energy to the workplace.
WARNING
Shock Hazard
Danger of electrocution. Good electrical grounding is essential to avoid
potentially serious shock hazards. A 3-wire outlet with ground connection
must be provided for the Cary 60. Make certain that power outlets are earth-
grounded at the grounding pin.
All power supplies for the Cary 60 must be single-phase, AC voltage, three-wire
system (active, neutral, earth) and should be terminated at an appropriate power
outlet receptacle that is within reach of the power cord. For safety reasons, a
separate power outlet receptacle should be provided for each unit in the system.
The use of extension cords or outlet adaptors is not recommended.
If necessary, replace the power cord only with a cord equivalent to the one
specified above.

Specifications
18 Agilent Cary 60 Spectrophotometer User’s Guide
Computer Requirements
The Cary 60 was evaluated under Agilent’s ISO9001 procedures using Cary WinUV
software and a desktop computer. We strongly recommend a computer
configuration that matches the system that was used during evaluations.
Processor
Intel Core i5 8500 (3.0 GHz, 9MB cache)
Memory
8GB (1x8GB) DDR4-2666 nECC RAM
Storage
500GB 7200 RPM SATA 6G Hard Drive
Graphics
Intel UHD Graphics 630
Communications
Integrated Intel I218LM PCIe GbE Controller; 2nd HP Serial Port Adapter
Optical Drive
9.5mm Slim DVD Writer
Audio
Integrated Conexant CX20632 5.1 HDA codec
USB
5 USB 3.0 ports, 3 USB 2.0
Locate the PC keyboard and mouse for ergonomically correct access.

Installation
Agilent Cary 60 Spectrophotometer User’s Guide 19
3Installation
Installation 19
Cell Holder 24
Other Sample Holders 26
Installing Accessories 26
Use the following checklist to make sure you have the work area ready to install
the Cary 60.
Preparation requirement
Complete
All equipment is on site and has been checked for damage.

The work area meets the environmental requirements (see Page 15).

A suitable workbench is available (see Page 16).

Suitable electrical power supplies are available (see Page 17).

A computer that meets requirements is available (see Page 18).

A working Microsoft Windows operating system is installed on the computer. For
instructions on installing this, refer to the documentation supplied with the operating
system.

The Cary 60 has been unpacked (see Page
20).

Installation
The Agilent Cary 60 spectrophotometer is designed to be completely customer-
installable. Instructions for setting up the system are included in the Cary 60
Installation Card supplied with the instrument and in the following sections.

Installation
20 Agilent Cary 60 Spectrophotometer User’s Guide
If you are installing the Agilent Cary WinUV Pharma software, follow the software
installation instructions provided with the software and then go to ‘Installing the
Hardware’ on Page 22.
Installation includes:
•Unpacking.
•Installing the Cary WinUV software.
•Installing hardware.
•Validating and registering your Cary 60 and Cary WinUV software.
•Running the tutorials.
Unpacking
WARNING
Heavy Weight
The Cary 60 weighs 18 kg. To avoid injury to personnel or damage to
equipment, always use two or more people when lifting or carrying the
instrument. NEVER attempt to lift the instrument alone.
After accepting delivery, take the equipment to the installation site. Agilent
instruments are inherently robust, and the packaging is designed to prevent
internal damage. However, the contents form part of a precision measuring
system and all packages should be handled with care. In transit, sharp jolts must
be avoided and the packages should not be inverted or tilted unnecessarily.
Markings on the shipping cartons generally indicate which side of the package
should be kept on top.
Unpacking the equipment is your responsibility. As the packages are opened,
ensure you received everything you ordered. If there are any discrepancies, notify
the supplier. If any items are found to be damaged, immediately notify the carrier
and supplier.
You should have received:
•1x Cary 60 spectrophotometer
•1x power supply and power cable
•1x USB cable
•1x Cary WinUV software installation UFD
•This manual