Agilent Technologies Cary 100 User manual

Cary
100/300/4000/5000/6000i
/7000 Spectrophotometers
User’s Guide

2 Cary 100/300/4000/5000/6000i/7000 Spectrophotometers User’s Guide
Notices
© Agilent Technologies, Inc. 2002,
2010-2013, 2016
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Manual Part Number
8510197200
Edition
Tenth edition, November 2016
Agilent Technologies Australia [M] Pty
Ltd.
679 Springvale Road Mulgrave,
Victoria, Australia. 3170
www.agilent.com
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Safety Notices
A CAUTION notice denotes a hazard.
It calls attention to an operating
procedure, practice, or the like that, if
not correctly performed or adhered to,
could result in damage to the product
or loss of important data. Do not
proceed beyond a CAUTION notice
until the indicated conditions are fully
understood and met.
A WARNING notice denotes a
hazard. It calls attention to an
operating procedure, practice, or the
like that, if not correctly performed or
adhered to, could result in personal
injury or death. Do not proceed
beyond a WARNING notice until the
indicated conditions are fully
understood and met.
WARNING
CAUTION

Contents
Cary 100/300/4000/5000/6000i/7000 Spectrophotometers User’s
Guide 3
Contents
1. Safety Practices and Hazards 5
Verifying Safe State 6
Ultraviolet radiation 6
Electrical hazards 6
Other precautions 8
Information symbols 9
Color coding 10
CE compliance 11
Electromagnetic compatibility 11
EN55011/CISPR11 11
ICES/NMB-001 12
South Korean Class A EMC declaration 12
2. Introduction 13
Installation requirements 13
Documentation 13
Conventions 14
Specifications 14
Electrical Connections 14
3. Installation 17
Starting the software 18
Software Status Bulletin 19
GPIB communications 19

Contents
4 Cary 100/300/4000/5000/6000i/7000 Spectrophotometers User’s Guide
USB-GPIB-HS converter 19
PCI-GPIB 20
Setting up the instrument 23
Cabling 23
Power 23
Turning on the system 24
Instrument performance tests 25
Sample holders 25
Cell holders 26
Solid sample holder (Cary 4000/5000/6000i/7000 only) 29
Installing the base plate 34
Installing other accessories 34
Nitrogen purging 35
Cary 100/300 35
Cary 4000/5000/6000i/7000 35
Removing the sample compartment base 38
Cary 100/300 38
Cary 4000/5000/6000i/7000 38
4. Maintenance 39
Cleaning 40
Source lamps 40
Fuses 41
Spare Parts 42

Safety Practices and Hazards
Cary 100/300/4000/5000/6000i/7000 Spectrophotometers User’s Guide 5
1. Safety Practices and Hazards
Verifying Safe State.....................................................6
Ultraviolet radiation .....................................................6
Electrical hazards.........................................................6
Other precautions ........................................................8
Information symbols....................................................9
Color coding .. .............................................................10
CE compliance ............................................................11
Electromagnetic compatibility .................................11
Your Agilent Cary instrument and accessories have been carefully
designed so that when used properly you have an accurate, fast,
flexible and safe analytical system.
If the equipment is used in a manner not specified by the
manufacturer, the protection provided by the equipment may be
impaired.
Information on safety practices appears throughout the
documentation (both printed and online) provided with your
instrument and accessories. Before using the instrument or
accessories, you must thoroughly read these safety practices.
Observe all relevant safety practices at all times.

Safety Practices and Hazards
6 Cary 100/300/4000/5000/6000i/7000 Spectrophotometers User’s Guide
Verifying Safe State
The following general safety precautions must be observed during all
phases of operation, maintenance and service of this instrument.
To ensure continued safety of the instrument after maintenance or
service procedures verify the instrument is returned to a safe state
for the user. This includes running performance checks to verify the
instruments safety systems are functioning correctly. Check the
general condition of the instrument during operation for wear or
signs of corrosion that are likely to inhibit function or safety.
Failure to comply with these precautions or with specific warnings
elsewhere in this manual violates safety standards of design,
manufacture, and intended use of the instrument. Agilent
Technologies assumes no liability for the customer’s failure to
comply with these requirements.
Ultraviolet radiation
The deuterium (standard in all instruments) and mercury lamps (if
fitted) in the instrument emit hazardous ultraviolet (UV) radiation.
This radiation can cause serious damage to eyes. NEVER look
directly at either lamp and NEVER operate either lamp unless it is
correctly mounted in the lamp turret (Cary 4000/5000/6000i/7000
only) and the turret is correctly mounted in the instrument.
NOTE The mercury lamp is fitted as standard in the Cary 4000/5000/6000i/7000.
Electrical hazards
The Cary 100/300/4000/5000/6000i/7000 instruments and some
accessories contain electrical circuits, devices, and components
operating at dangerous voltages. Contact with these circuits, devices
and components can cause death, serious injury, or painful electrical
shock.

Safety Practices and Hazards
Cary 100/300/4000/5000/6000i/7000 Spectrophotometers User’s Guide 7
Panels or covers which are retained by screws on the
spectrophotometer and accessories (with the exception of the lamp
access cover) may be opened ONLY by Agilent-trained, Agilent-
qualified, or Agilent-approved service engineers. Consult the manuals
or product labels supplied with your computer, monitor and printer
to determine which parts are operator-accessible.
Operators and other unauthorized personnel are permitted access
ONLY to the lamp compartment and the sample compartment of the
Cary. ALWAYS switch off the spectrophotometer before changing a
lamp in the lamp compartment.
Application of the wrong supply voltage can create a fire hazard and
a potentially serious shock hazard, and could seriously damage the
Cary system, accessories and any attached ancillary equipment. The
Cary 4000/5000/6000i/7000 has a Universal Power Supply that
adapts to the supply voltage. However, care must be taken to ensure
that the correct voltage is used.
Do not connect the spectrophotometer or accessories to the mains
power supply until you have made sure that the slide switch(es)
(Cary 100/300 only) at the rear of these are correctly set for the
mains power supply in the specific outlet in your laboratory to which
the equipment will be connected. Consult the manuals supplied with
your computer, monitor and printer for their specific voltage
requirements.
Replace blown fuses with fuses of the size and rating as stipulated in
the text adjacent to the fuse holder or in the manuals where listed.
Do NOT use power cords with faulty or frayed insulation.
When replacing the mains power cord, only use a mains power cord
with a rating equivalent to the one supplied with the instrument. See
the Site Preparation Guide for the instrument for detailed mains
power cord rating information.
Do not position the equipment so that it is difficult to operate the
disconnecting device.

Safety Practices and Hazards
8 Cary 100/300/4000/5000/6000i/7000 Spectrophotometers User’s Guide
Other precautions
Both the deuterium and the visible lamps operate at high
temperatures, and touching either of these lamps may result in
burns. Before replacing a lamp that has been lit, switch off the
spectrophotometer and either ensure that the lamp has cooled, or
protect your fingers from burns.
Do not block the ventilation grilles on the spectrophotometer and
accessories. Consult the manuals supplied with your computer,
monitor and printer for their specific ventilation requirements.
Use of the Cary system and accessories may involve materials,
solvents and solutions which are flammable, corrosive, toxic or
otherwise hazardous.
Careless, improper, or unskilled use of such materials, solvents and
solutions can create explosion hazards, fire hazards, toxicity and
other hazards which can result in death, serious personal injury, and
damage to equipment and property.
ALWAYS ensure that laboratory safety practices governing the use,
handling and disposal of such materials are strictly observed. These
safety practices should include the wearing of appropriate safety
clothing and safety glasses.

Safety Practices and Hazards
Cary 100/300/4000/5000/6000i/7000 Spectrophotometers User’s Guide 9
Information symbols
The following triangular symbols appear in conjunction with
warnings on the spectrometer and associated documentation. The
hazard they depict is shown below each symbol:
Broken glass Corrosive liquid
Ejecting parts
Electrical shock Eye hazard Fire hazard
Heavy weight
(danger to feet)
Heavy weight
(danger to hands)
Hot surface
Moving parts
Noxious gas
The following symbol may be used on warning labels attached to the
instrument. When you see this symbol, refer to the relevant operation
or service manual for the correct procedure referred to by that
warning label.

Safety Practices and Hazards
10 Cary 100/300/4000/5000/6000i/7000 Spectrophotometers User’s Guide
The following symbols also appear on the instrument or in the
documentation:
IMains power on
0Mains power off
Fuse
Single phase alternating current
Focus
Vertical adjustment
Horizontal adjustment
Caution, disconnect all supplies, risk of electric shock
High leakage current – ensure proper grounding
Color coding
The various indicator lights appearing on Agilent instruments and
associated accessories are color-coded to represent the status of the
instrument or accessory.
 A green light indicates the instrument is in normal/standby
condition.
 An orange light indicates that a potential hazard is present.
 A blue light indicates that operator intervention is required.
 A red light warns of danger or an emergency.