Agilent Technologies 2100 Bioanalyzer System Operating instructions

Agilent Technologies
Agilent 2100 Bioanalyzer
System
Maintenance and Troubleshooting
Guide

Maintenance and Troubleshooting
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Manual Part Number
G2946-90003 Rev. B
Edition
02/2017
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Maintenance and Troubleshooting 3
In this Book
In this Book
This manual provides maintenance and troubleshooting information for the
Agilent 2100 Bioanalyzer system. It includes essential measurement practices,
troubleshooting hints for hardware, software and applications, maintenance
procedures and a list of spare parts and accessories.
This manual is based on the 2100 Expert Software revision B.02.08. Other
software revisions may have an impact on results.

4 Maintenance and Troubleshooting
Contents
Contents
1 Essential Measurement Practices 7
Overview 8
Tools and Handling 9
Chip Priming Station 10
Reagents and Reagent Mixes 11
Samples 12
Chips 13
Agilent 2100 Bioanalyzer System 14
2 Troubleshooting the Instrument Communication 15
Verify the Instrument Communication 16
Troubleshooting Communication Issues 18
Changing COM Port Settings 20
USB to Serial Adapter 21
3 Troubleshooting the 2100 Expert Software 25
Run Installation Qualification Test 26
4 Hardware Diagnostics 29
Overview 30
Diagnostic Test Procedure 32
5 Troubleshooting the DNA Application 35
Overview 36
Symptoms (DNA) 37
Symptoms (High Sensitivity DNA) 62
6 Troubleshooting the RNA Application 67
Overview 68
Symptoms (RNA) 69

Maintenance and Troubleshooting 5
Contents
7 Troubleshooting the Protein Application 87
Overview 88
Symptoms (Protein) 89
Symptoms (High Sensitivity Protein) 112
8 Maintenance of the Electrode Cartridge 115
Overview 116
DNA and Protein Assays 117
RNA Nano Assay 119
RNA Pico or Small RNA Assay 123
How to Clean the Pin Set of the Electrode Cartridge 126
9 Maintenance of the Chip Priming Station 131
Overview 132
Replacing the Syringe 133
Cleaning the Syringe Adapter 134
Replacing the Syringe Adapter 136
Replacing the Gasket 137
Checking the Chip Priming Station for Proper Performance - Seal Test 139
10 Maintenance of the Agilent 2100 Bioanalyzer instrument 141
Overview 142
Cleaning the Lens 143
Changing the Fuses 144
11 Maintenance of the Vortexer 149
Changing the Adapter 150
12 Spare Parts and Accessories 151
Overview 152

6 Maintenance and Troubleshooting
Contents

7
Maintenance and Troubleshooting
Agilent Technologies
1
Essential Measurement Practices
Overview 8
Tools and Handling 9
Chip Priming Station 10
Reagents and Reagent Mixes 11
Gel and Gel-Dye Mix 11
Samples 12
Chips 13
Agilent 2100 Bioanalyzer System 14

8 Maintenance and Troubleshooting
1 Essential Measurement Practices
Overview
Overview
This section lists all user relevant hints on handling tools, chips, reagents and
the Agilent 2100 Bioanalyzer system. For the latest information on
assay-related hints, go to the Agilent web site at:
www.agilent.com/genomics/bioanalyzer

Maintenance and Troubleshooting 9
Essential Measurement Practices
1
Tools and Handling
Tools and Handling
•Always follow the GLP-rules established in the laboratory.
•Always wear gloves when handling chips to prevent contamination.
•When pipetting sample, use non-filter pipette tips that are of adequate size.
Pipette tips that are too large will lead to poor quantitation accuracy.
•Change pipette tips between steps to avoid cross-contamination.
•Always insert the pipette tip to the bottom of the well when dispensing
liquid. Placing the tip at the edge of the well leads to bubbles and poor
results. Holding the pipette at a slight angle will ensure proper dispensing
of the liquid.
•Use a new syringe and electrode cleaner with each new kit.

10 Maintenance and Troubleshooting
1 Essential Measurement Practices
Chip Priming Station
Chip Priming Station
•Refer to the appropriate Kit Guide for the correct position of the syringe
clip and base plate.
•Replace the syringe with each new kit.
•Check the performance of the chip priming station by applying the seal test
on a monthly basis. For details see “Maintenance of the Chip Priming
Station” on page 131. If necessary, replace the gasket and/or adapter.