Agilent Technologies 8453 User manual

s1
Temperature Control
Accessory for
Agilent 8453 UV-visible
Spectrophotometer
Operator’s Manual

Agilent Technologies Deutschland GmbH
Hewlett-Packard-Strasse 8
76337 Waldbronn
Germany
Copyright Agilent
Technologies 2000
All rights reserved.
Reproduction, adaption,
or translation without
prior written permission
is prohibited, except as
allowed under the
copyright laws.
Part No. 89090-90002
Edition 02/00
Printed in Germany
Warranty
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contained in this
document is subject to
change without notice.
Agilent Technologies
makes no warranty of
any kind with regard to
this material,
including, but not
limited to, the implied
warranties or
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fitness for a particular
purpose.
Agilent Technologies
shall not be liable for
errors contained herein
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consequential damages
in connection with the
furnishing, performance,
or use of this material.
WARNING
For details of safety,
see Safety Information
on page 191.
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In This Book
The apparatus is marked
with this symbol when
the user should refer to
the instruction manual
in order to protect the
apparatus against
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Operator’s Manual
Temperature Control Accessory for Agilent 8453 UV-visible
Spectrophotometer

4
In This Book
This handbook describes operation, installation, maintenance and repair,
using GPIB commands, and service of the temperature control accessory.
The handbook has four parts.
Part 1 “Installing and Maintaining Your Temperature Controller” describes
how you install, and maintain your temperature control unit, cell holder
(option #100), external sensor (option #200) and GPIB commands PROM
(option #305).
Part 2 “Using Your Temperature Controller” describes how you operate your
temperature controller; that is the temperature control unit with the cell
holder (option #100) and external sensor (option #200). There is also a
description of what you do in response to the messages that the control unit
displays.
Part 3 “Using GPIB Commands with the Temperature Controller” is intended
to provide information necessary to interface the temperature control unit to
various computer systems, for option #305. It is written from a software
interfacing point of view and assumes that the reader is knowledgeable in
software interfacing concepts.
Part 4 “Servicing the Temperature Controller” is intended only for use by
Agilent Technologies service personnel or other qualified engineers. It
contains a description of the electronics, and information about the service,
cables and connectors, and replacement parts of your temperature
controller.

Contents
5
Part 1 Installing and Maintaining Your Temperature Controller
1 Description
An overview of the temperature control accessory, including
specifications 17
Temperature Control Unit 19
Temperature-Controlled Cell Holder 20
External Sensor 21
GPIB Commands 22
2 Installing Your Controller
How to install your control unit, cell holder, external sensor
and GPIB commands PROM 23
Choosing a Suitable Place 24
Unpacking Your Controller 25
Unpacking the Controller Options 26
Setting Up Your Control Unit 27
Viewing the Control Unit 28
Selecting Voltage 29
Selecting Fuse 30
Connecting Cables 32

6
Contents
Setting Up the Temperature-Controlled Cell Holder 33
Removing the Multicell Transport Cell Holder 33
Removing other Cell Holders 34
Viewing the Cell Holder 34
Installing the Temperature-Controlled Cell Holder 34
Connecting Cables 35
Connecting Flow Cell and Heat Exchanger 36
Setting Up Your External Sensor 38
Viewing the External Sensor 39
Connecting Cables 39
Installing Option #305 for GPIB Commands 40
Installing GPIB Commands PROM and GPIB Cable 40
3 Simple Maintenance
Maintenance tasks you can do on your control unit and cell
holder 43
Changing Fuse 46
Line (Primary) Fuse 46
Internal (Secondary) Fuse 46
Leaking Fittings on Heat Exchanger 49
Stage 1: Disassembling Heat Exchanger 49
Stage 2: Changing Ferrule on Fittings 49
Stage 3: Changing Fitting 50
Stage 4: Reassembling Heat Exchanger 51
Cleaning Cells 53
Removing Cell 53
Replacing Cell 55
Cleaning Heat-Exchanger Tubing 56

Contents
7
Part 2 Using Your Temperature Controller
4 Operating Your Control Unit
How to prepare your control unit for operation 59
Before you Start 61
Turning on Line Power 62
Checking Operation 63
Turning off Line Power 64
Using Functions 65
Selecting Functions 65
Setting Functions 66
Resetting Functions 66
Setting Temperature 67
Example for Setting Temperature 67
Example for Correcting a Wrong Set Temperature 68
Changing Brightness 69
Changing Units 70
Turning off Peltier Element 72
Selecting Stirring 73
Setting System Functions 74
Setting Remote Mode 74
Changing GPIB Address 75
Starting Self-Test Manually 76
Hints for Operation 77

8
Contents
5 Display Summary
A summary of the different control and read-out functions 79
Normal Mode 80
Edit Mode 80
6 Using External Sensor
How to get more accurate temperature monitoring of your
sample 81
7 Messages
The messages that the control unit displays 85
Responding to Messages 87
OVERLOAD! 87
WARNING 1 87
WARNING 2 89
Display Messages 90
OUT OF RANGE 90
Status Messages 91
Status Lamps 92
Ready 92
Error 92
Stirrer 92

Contents
9
Part 3 Using GPIB Commands with the Temperature Controller
8 Communicating GPIB Commands
Controlling and communicating with the temperature control
unit 95
Communication Interface 96
Remote Mode 97
GPIB Control 98
Service Request 99
Trigger Lines 99
Device Clear 99
9GPIBCommands
The GPIB commands for controlling the temperature control
unit 101
Buffers 102
Instruction Format 103
General Description 103
Detailed Description 104
CSM –Agilent ChemStation Mode 105
ERR –Error 107
EXT –External Temperature 111
IDY –Identify 112
MSK –Service Request Mask 113
PEL –Peltier 115

10
Contents
REM –Remote 116
SET –Set Cell Temperature 117
SEU –Select Unit 118
SPE –Speed 119
STA –Status 120
STR –Stirrer 122
TEM –Cell Temperature 123
TRA –Trigger Acknowledge 124
TRG –Trigger Out 125
TST –Test 126

Contents
11
Part 4 Servicing the Temperature Controller
10 Electronics of the Temperature Control Accessory
Block-diagram-level descriptions of the electronics 131
Electronics Overview 134
Power Supply 136
Line Fuses 136
Internal Fuses 136
Transformer, Regulation 136
Power Fail Detector 136
Power On Detector 137
GND, AGND, PGND, SGND, Shield 137
Microcontroller and Peripherals 139
PROM Card 139
Latch, Remote Control Connector 139
Lines to Power Amplifiers 139
Lines to Sensor Electronic 140
EEPROM 140
Stirrer Driver, Stirrer 140
Reset, NMI 140
Keyboard Display Electronic 142
Keyboard 142
LEDs 142
Control Logic 142
8-Character Display 142
Power Amplifier 144
Switch Controller, Current Sensor 144
Switch 144
H-Bridge, Peltier Element 144

12
Contents
Sensor Electronic 146
Current Source 146
Multiplexer, Demultiplexer 146
Measuring Principle 147
+5 V Regulator, Voltage Divider 148
Analog to Digital Converter (ADC) 148
11 Cables and Connectors
Cables used with the temperature controller. 149
Cables 150
Connectors 153
12 Troubleshooting
Troubleshooting instructions for trained personnel. 159
General Description 161
Diagnostic During Operation and Power-On 161
Starting Self-Test Manually 161
Basic Failures 162
Error Messages 164
13 Identifying and Replacing Parts
How to find parts information. 175
Parts Identification 178

Contents
13
Changing Parts 181
Changing Temperature Controller Board 181
Changing Inner Cabinet Assembly 182
Changing Cell Holder Cable 183
Legal, Safety and Warranty Information
Warranty Statement 188
All Chemical Analysis Products 188
Limitation of Warranty 188
Limitation of Remedies and Liability 189
Responsibilities of the Customer 189
Responsibilities of Agilent Technologies 190
Safety Information 191
General 191
Operation 191
Safety Symbols 192
Radio Interference 195
Manufacturer’s Declaration 195
Herstellerbescheinigung 195
Agilent Technologies on Internet 196

14
Contents

Part 1
Part 1 Installing and Maintaining Your
Temperature Controller
1 Description 17
2 Installing Your Controller 23
3 Simple Maintenance 43

16
Installing and Maintaining
Your Temperature
Controller
This part describes how you install, and maintain your temperature control
unit, cell holder (option #100), external sensor (option #200) and GPIB
commands PROM (option #305). The information for the cell holder, controller
and external sensor is divided into the following chapters.
❏Chapter 1 “Description”
❏Chapter 2 “Installing Your Controller”
❏Chapter 3 “Simple Maintenance”

1
1Description
An overview of the temperature control
accessory, including specifications

18
Description
The temperature control accessory consists of the control unit and
temperature-controlled cell holder for the Agilent 8453 UV-visible
spectrophotometer for sample temperature control. The digitally controlled
unit uses thermoelectric heating and cooling (Peltier principle) over a 60 °
temperature range, 10 °C –70 °C at 20 °C. The temperature control accessory
gives a stable temperature for a wide range of applications, for example,
enzyme kinetic rate determinations and equilibrium studies.
The Peltier temperature control accessory consists of four parts.
❏temperature control unit
❏temperature-controlled cell holder
❏external sensor (optional)
❏GPIB commands (optional)

19
Description
Temperature Control Unit
Temperature Control Unit
The control unit gives sample temperature control, sample stirring, and
status and error indication. It controls the temperature of the cell holder and
is operated manually through the keyboard interface or remotely through
GPIB commands.
Table 1 Control Unit Technical Specifications
Power supply 100–120 VAC, 50–60 Hz
220–240 VAC, 50–60 Hz
Temperature set range -10 °C–120 °C *
Temperature set resolution 0.1
Temperature Display Range -10 °C–120 °C **
Display resolution 0.1
Electromagnetic stirrer speed range 40–1000 rpm
* or equivalent in K or °F
** or equivalent in K or °F

20
Description
Temperature-Controlled Cell Holder
Temperature-Controlled Cell Holder
The cell holder is a temperature-controlled cell holder for 1 cm sample cells
(cuvettes) and flow cells.
Table 2 Cell Holder Technical Specifications
Operating temperature range 10 °C–70 °C at 20 °C ambient temperature
Accessible temperature range 10 °C below ambient temperature
Temperature stability 50 °C above ambient temperature
10 °C–60 °C ±0.1K
>60 °C, ±0.2K
Temperature accuracy 0 °C–<20 °C, ±0.3K
20 °C–40 °C, ±0.2K
>40 °C–60 °C, ±0.3 K
Temperature Reproducibility 0 °C–60 °C, ±0.1 K
>60 °C, ±0.2K
Typical Step Response 5.5 °C/min heating
3.0°C/min cooling