Agilent Technologies Cary 8454 User manual


Agilent Cary 8454
UV-VisibleSpectroscopy
System
Operator’s Manual

Agilent Cary 8454 UV-Visible Spectroscopy System Operator’s Manual
Notices
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2003-2008, 2011, 2013, 2014, 2016
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Manual Part Number
G1115-90002
Edition
09/16
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Safety Notices
CAUTION
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minor revisions of the software that do not
affect the technical accuracy of this hand-
book.

Agilent Cary 8454 UV-Visible Spectroscopy System Operator’s Manual 3
In This Guide…
This book is an introduction to your Agilent Cary 8454 UV-Visible
spectroscopy system. It includes step-wise procedures and examples for basic
operations and tasks.
For more detailed information see the following manuals:
• Agilent Cary 8454 UV-Visible Spectroscopy System Installation Guide
• Understanding Your UV-Visible Spectroscopy System
• Agilent Cary 8454 Service Manual
• UV-Visible ChemStation Help
1 Safety Information
Introduces your UV-Visible spectrophotometer and the Agilent ChemStation
software.
2 Introduction to Your System
Introduces your UV-Visible spectrophotometer and the Agilent ChemStation
software.
3 Installation and Start Up
Summarizes the system installation process, and explains how to start a
measurement session.
4 Good Measurement Practices
Provides guidance for good measurement practices.
5 Using your UV-Visible Spectroscopy System
Provides examples of basic measurements and related tasks.

4 Agilent Cary 8454 UV-Visible Spectroscopy System Operator’s Manual

Agilent Cary 8454 UV-Visible Spectroscopy System Operator’s Manual 5
Agilent Cary 8454 Contents
1 Safety Information 9
Safety Information 10
Verifying Safe State 10
Warning Symbols 10
Precautions 12
Solvent Information 13
Flow Cells 13
Solvents 13
Other Precautions 15
Protective Earth 15
Disconnecting Device 15
Allowing Hot Instrument to Cool 16
Environmental Conditions and Power Requirements 17
Pollution level 18
Regulatory Information 18
Electromagnetic Compatibility 18
2 Introduction to Your System 21
Agilent Cary 8454 UV-Visible Spectrophotometer — Overview 22
Optical System Overview 22
Spectrophotometer Description 26
General Purpose Agilent ChemStation Software for UV-Visible Spectroscopy —
Overview 30
User Interface Elements 31
Software Structure 35
Standard Mode Tasks 37

6 Agilent Cary 8454 UV-Visible Spectroscopy System Operator’s Manual
Contents
Standard Mode Data Processing 40
3 Installation and Start Up 47
Installation Summary for Your General Purpose UV-Visible System 48
General 48
Spectrophotometer 48
PC 49
Starting a Measurement Session 50
4 Good Measurement Practices 51
General Considerations 52
Spectrophotometer Design 52
Making Measurements 52
Sample Cell Material 53
Optical Specifications of Cells 54
Apertured Cells 55
Flow Cells 56
Handling and Maintaining Cells 57
Solvents 59
Sample Preparation 60
Photosensitive Samples 61
Stirring and Temperature Control 62
Checklist for Best Results 62
Inserting a Cell 65
5 Using your UV-Visible Spectroscopy System 67
Starting Your First Measurement Session 68
Starting Your UV-Visible Software 70
Measuring Caffeine Absorbance at 273 nm 71
Saving Your Parameters as a Method 74
Retrieving and Printing a Method 76

Contents
Agilent Cary 8454 UV-Visible Spectroscopy System Operator’s Manual 7
Saving and Retrieving Data 79
Saving your Samples 79
Saving a Selected Spectrum 81
Retrieving Spectra 83
Deleting Current Spectra 84
Print Preview of Reports 85
Finding the Caffeine Absorbance Maximum 88
Entering your Cell’s Path Length 92
Controlling your Sipper System 93
Using your Multicell Transport 95
Quantitative Analysis using a Calibration with Standards 98
Setup 99
Calibration 101
Analysis 103
How Can I Be Sure That My Spectrophotometer Works Properly? 105
Spectrophotometer Self test 105
What Maintenance Does My Spectrophotometer Require? 107
Cleaning 107
Spare Parts 107
How Can I Get a Deeper Understanding of UV-Visible Spectroscopy? 108
When Do I Have to Measure a Blank? 110
Index 111

8 Agilent Cary 8454 UV-Visible Spectroscopy System Operator’s Manual
Contents

9
Agilent Cary 8454 UV-Visible Spectroscopy System
Operator’s Manual
Agilent Technologies
1
Safety Information
Safety Information 10
Warning Symbols 10
Precautions 12
Solvent Information 13
Other Precautions 15
Environmental Conditions and Power Requirements 17
Regulatory Information 18

10 Agilent Cary 8454 UV-Visible Spectroscopy System Operator’s Manual
1 Safety Information
Safety Information
Safety Information
Agilent products must only be used in the manner described in the Agilent
product user guides. Any other use may result in damage to the product or
personal injury. Agilent is not responsible for any damages caused, in whole
or in part, by improper use of the products, unauthorized alterations,
adjustments or modifications to the products, failure to comply with
procedures in Agilent product user guides, or use of the products in violation
of applicable laws, rules or regulations.
Verifying Safe State
The following general safety precautions must be observed during all phases of
operation, maintenance and service of this instrument. To ensure continued
safety of the instrument after maintenance or service procedures, verify the
instrument is returned to a safe state for the customer and service personnel
user. This includes running performance checks to verify the instruments
safety systems are functioning correctly. Check the general condition of the
instrument during operation, and for wear or signs of corrosion that are likely
to inhibit function or safety.
Warning Symbols
To avoid personal injury, death, or damage to the instrument, the operator
must refer to an explanation in the manual. This symbol is placed on the
product where it is necessary for you to refer to the manual in order to
understand a hazard.