AmScope M620 Series User manual

M620
Series
1
User’s Manual

2
M620
Series
T
abl
e
o
f
Contents
Before Use........................................................................................................... 3
Introduction.............................................................................................3
Safety Precautions ..................................................................................4
Parts............................................................................................................... 5
M620.................................................................................................5
Definition of Parts ............................................................................... 6
Getting Started ...........................................................................................7
Assembly.................................................................................................7
Operation ...................................................................................................8
Setting Up.................................................................................................. 8
Focusing ..................................................................................................8
Adjusting The Diaphragm................................................................... 8
Attaching a Camera / Changing Eyepieces...........................................8
Setting the Stage’s Stop-Limit ...............................................................9
Maintenance/Precautions .................................................................... 10
Specifications..............................................................................................11
Specifications....................................................................................... 11
Optional Accessories........................................................................... 12
Objectives..........................................................................................13
Eyepieces ............................................................................................. 13
Technical Parameters ...............................................................................14
Electrical System...............................................................................14
Technical Terms & Concepts ..........................................................15
Troubleshooting.......................................................................................16
Common Issues.................................................................................... 16
Common Issues (Continued).............................................................17

3
M620
Series
Before
Use
Introduction
Congratulations on the purchase of your new AmScope microscope! This manual is designed for the
M620 series microscopes. It comes with a professional monocular head, double layer mechanical
stage, coarse & fine focusing, and LED illumination. There are eight magnification settings: 40X,
64X, 100X, 160X, 400X, 640X, 1000X & 1600X. This microscope is an ideal instrument for bacterial,
biological, and pharmaceutical researches.
Please be sure to take a few minutes to familiarize yourself with the features and functions of your new
AmScope microscope.
If you’d like more information on microscopes, parts, or accessories, please visit our website at:
www.amscope.com
We highly recommend you study this manual thoroughly before operating the microscope and that
you keep it on hand for future reference.
If you have additional questions or need assistance, please send us an email at:
[e-mail]

4
M620
Series
Before
Use
Safety Precautions
1. As the microscope is a precision instrument, always handle it with care, avoiding impact
or abrupt movement during transportation. Do not shake the package.
2. Do not place the microscope in direct sunlight or in high heat. Keep it indoors in a dry
and clean place with temperatures between 32-100 °F (0-40 °C), and in maximum
relative humidity of 85%.
3. Avoid touching the lenses on the objectives and the eyepieces so that oil and dirt from
your fingerprints do not obstruct your view.
4. Before turning the power on, make sure that the power supply voltage is consistent with
the voltage of your microscope.

5
M620
Series
Parts
M620
Eyepiece
Ocular Tube
Rotating Monocular Head
Nosepiece Turret
Objectives
Limit Stop
Stage
StageClips
Condenser
Fine Focusing
Knob
Illuminator Lens Coarse Focusing
Knob

6
M620
Series
Parts
Definition of Parts
BaseIlluminatorLens
Directs the light source towards the slide
Focusing Knob
Used to bring the slide into the sight and focus
Disc Iris Diaphragm
Controls the amount of light that hits the slide from the base illuminator
Dimmer
Controls the amount of light that escapes from the base lens
Limit Stop Knob
Limits the upward movement of the mechanical stage in order to avoid damaging the slide and objective
Nosepiece
Houses the objective lenses
Rotating Monocular Head
Houses the eyepiece and optics of the microscope

7
M620
Series
Getting
Started
Assembly
1. First, take the styrofoam container out of the cardboard carton and lay it on its side, paying attention
to which side is labeled up. Remove the tape and open the container carefully so as to avoid dropping
and damaging the optical items. Check carefully to ensure that all parts and accessories are intact.
2. Check the packing list to ensure that you’re received all items:
- One Monocular Microscope Body & Head
- Four Objectives (4x, 10x, 40x, 100x)
- 10x Widefield Eyepieces
- 16x Widefield Eyepieces (for -A models only)
- 20x Widefield Eyepieces (for -B models only)
- 25x Widefield Eyepieces (for -C models only)
- One Dust Cover
Note: LED models do not have a spare bulb, as the LED bulbs do not need replacing.
3. Remove the microscope body from the box and remove the plastic protective covering. The body of
the micro- scope is composed of the base, the stage, the arm, and the nosepiece.
4. Screw the objectives into the microscope nosepiece from the lowest magnification to the highest,
again avoiding touching the lenses.
5. Plug in the microscope and turn it on. If no light emerges from the light source, adjust the
dimmer knob on the side of the base.

8
M620
Series
Operation
Setting Up
1. Place the specimen to be studied on a glass slide (or use a prepared slide). Place it on the stage,
holding it snugly in place with the metal slide holders (clips) of the mechanical stage.
2. Center the specimen over the stage opening, lining it up with the light and the objective lens.
3. To adjust the illumination, slowly turn the dimmer on the right side of the base until the desired
intensity of light is achieved.
Focusing
1. Turn the nosepiece to choose an objective. It is easiest to use the lowest magnification first (4x
objective) to lo- cate and focus on the specimen. As you move up in magnification you may need to
refocus the image a little each time.
2. Being focusing by first looking with one eye through the eyepiece without the diopter. Close your
other eye. Use the coarse focusing knob to adjust the height of the stage until the sample comes into
clear focus.
Note: You may loosen the limit-stop knob (located on the read of the stage) in order to give yourself the
full range of motion for fine tuning the focus.
3. Once the image is clear in your field of view, you will want to use the fine focusing knob to tune it for
best results.
Note: Please be careful when moving the mechanical stage if you need to re-center the sample, or if
moving the stage very close to the objectives. The limit stop is designed to prevent impact between
objective and slide, so when it is off you will be able to damage the microscope.
Adjusting the Diaphragm
1. By changing the aperture (hole size) of the iris diaphragm, you can adjust the background brightness.
Adjust the aperture of the iris diaphragm by rotating the disc underneath the stage to the desired
aperture.
Attaching a Camera / Changing Eyepieces
1. To remove the 10x eyepiece included on the microscope, you will need to remove the small metal
screw on the ocular tube just under where the eyepiece connects to the tube. To do so, you will need a
1mm flathead precision screwdriver (much like what you would use to adjust eye glasses).

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M620
Series
Operation
Setting the Stage’s Stop-Limit
1. To adjust the stop limit on the stage, unlock it by unscrewing the lower nut. The stop limit is located at the rear
of the stage.
2. Once tension is released on the nut by rotating it counter clockwise, you may adjust the upper thumb screw to
move the limit up or down as desired.
3. Lock the limit stop nut back into place after the desired stage height is achieved.

M620
Series
Operation
10
Maintenance/Precautions
- All glass surfaces must be kept clean. Fine dust on the optical surface should be blown off using a can of com-
pressed air or gently wiped off with a soft lens paper tissue/nonabrasive lint free cloth.
- Carefully wipe off oil or fingerprints on the lens surfaces using tissue moistened with a small amount of lens
cleaner (we recommend Sparkle brand optical cleaner).
- Do not use Sparkle to clean other elements of the microscope. Use a neutral detergent on any plastic or painted
surfaces.
- Do not assemble or disassemble the microscope’s electrical components yourself without advisement from one of
our technicians. Doing so will void your warranty unless by advisement of one of our technicians to do so.
- After use, cover the microscope with the provided dust cover.
- Keep your AmScope microscope in a dry, clean location in order to prevent rust or other damage.
- To change batteries with this unit, use the included allen wrench (hexagonal shaped L-tool) to undo the door hex
screw on the base. Use 3 AAA batteries with the unit.
- The unit can be used as a recharger for AAA batteries specified as rechargeable only. Please do not use standard
batteries in the unit with the charger plugged in to avoid damage.

M620
Series
Specifications
11
620 Series Specifications
Parts Specifications M620 M620A M620B M620C
WF Eyepiece WF10X/18mm
WF10X/18mm w/ Pointer xxxx
WF10X/18mm w/ Reticle
WF16X/18mm x
WF20X/18mm x
WF25X18mm x
Plan Eyepiece P5X
P10X
P16X
DIN Achromatic Objectives 4X/0.10 xxxx
10X/0.25 xxxx
40X(spring)/0.65 xxxx
60X(spring)/0.85
100X(spring,oil)/1.25
Plan Objectives 4X
10X
40X(spring)
100X(spring, oil)
45 Degree Viewing Head Binocular Sliding, 360 Degree Swiveling
Trinocular Sliding, 360 Degree Swiveling
Monocular, 360 Degree Swiveling xxxx
Diaphragm Iris Diaphragm Disc xxxx
Illumination Halogen Light w/ Dimmer
LED Light w/ Dimmer xxxx
Lamp 6V/20W
6V/30W
LED xxxx
Filter Blue/Yellow/Green

M620
Series
Specifications
12
Optional Accessories
Parts Description Model # Purpose
Eyepiece 5X EP5X23 Obtaining 20x, 50x, 200x, and 500x magnification
powers
20x EP20X23 Obtaining 80x, 200x, 800x, and 2000x magnifica-
tion powers
25x EP25X23 For obtaining 250x and 2500x magnification pow-
ers
10x w/ Pointer EP10X23P For easier identifying of objects
10x w/ Reticle EP10X23R For measuring objects
Objective 2X A2X For obtaining 20x and 32x magnification powers
5X A5X For obtaining 50X and 80X magnification powers
20X A20x For obtaining 200x and 320x magnification powers
60X A60X For obtaining 600x and 960x magnification powers
Plan 4X PA4X For obtaining higher clarify in images
Plan 10X PA10X For obtaining higher clarify in images
Plan 40X PA40X For obtaining higher clarify in images
Plan100X PA100X For obtaining higher clarify in images
Camera CMOSDigital MU035 (350k)
MU130 (1.3mp)
MU300 (3mp)
MU500 (5mp)
MU800 (8mp)
MU900 (9mp)
MU1000 (10mp)
To capture images, video, or view live display on a
computer (PC/Mac OS X)
Calibration Micrometer MR400 To calibrate the camera software for on screen
measurements
CCD TV/Video (Trinocular
Only)
CCD-NP To view live display on a television (RCA)
Case Aluminum Case AC-B100 For carrying microscope around safely
Darkfield Condenser Dry Darkfield Condenser DK-DRY100 For low magnification darkfield work
Oil Darkfield Condenser DK-OIL100 For high magnification darkfield work

M620
Series
Specifications
13
Objectives
Type Magnification Numerical Aperture
(N.A.)
Medium Parfocal Distance (mm) Magnification Marks
(Color Ring)
DIN Achromatic
Objective (195mm)
4X A2X Air 45 Red
10X A5X Air 45 Yellow
40X A20x Air 45 LightBlue
60X A60X Air 45 Deep Blue
100X A100X Cedar Oil 45 White
Plan Objective
(195mm)
Plan 4X PA4X Air 45 Red
Plan 10X PA10X Air 45 Yellow
Plan 40X PA40X Air 45 LightBlue
Plan 100X PA100X Cedar Oil 45 White
Eyepieces
Type Widefield Eyepiece
Medium
Plan Eyepiece
Magnification 10X 15X 20X 5X 10X 16X
Field of View
Φ 18 Φ 13 Φ 11 Φ 18 Φ 18 Φ 15

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M620
Series
T
echnica
l
Parameters
Electrical System
There are two options for electrical systems for this series of microscope. The light source is an LED system.
1. 220V~240V power supply: 220V~240V ±10%, 50Hz
This electrical system is CE and GS certified
2. 100V~120V power supply: 100V~120V ±10%, 60Hz
This electrical system is UL certified.
All units come standard as 110V units unless an upgrade to a 220V system is requested. Upgrade fee is dependent
on which unit is purchased.
This unit is also capable of using batteries and charging rechargeable batteries. It uses 3 AAA batteries.
When plugged in with batteries in the unit, the batteries will be charged.
Note: Please do not use the recharging feature with standard batteries, as damage to your unit can occur.

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M620
Series
T
echnica
l
Parameters
Technical Terms & Concepts
TotalMagnification
Total magnification of a microscope is calculated by the magnification of the objective multiplied by the
magnification of the eyepieces.
-Ex: (10x Eyepieces) x (4x Objective) = 40x Total Magnification
Field of View
Linear field of view of the eyepiece divided by the magnification of the objective
Numerical Aperture (N.A)
Calculated by n Sin α (max), the Numerical Aperture (N.A) is an important parameter that marks the
features of the objective and condenser’s image quality and resolution. The “n” is a refractive index of the
medium (air or immersion cedar oil) between the objective lens and the specimen. The “ α ” is 1/2 of the angle
between the aperture on the objective and path of light. The larger the N.A, the higher the resolution of the
objective (and better quality of the image) is.
Object to Primary Image Distance
The distance between the object plane and the primary image plane. The conjugate distance is fixed.
Mechanical Tube Length
The distance between the objective shoulder and the ocular shoulder

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M620
Series
T
roubleshooting
Common Issues
Symptom Cause Remedy
OPTICAL ISSUES
One side of the field
of view is darker
The nosepiece if misaligned Turn the nosepiece until it clicks into place
Stains or dust has accumulated on the objective,
eyepieces, or base lens
Clean all lenses with lens cleaner or a lint free non-
abrasive cloth
Obstructions are
observed in the field
of view
Stains, dust, or dirt has accumulated on the speci-
men
Clean the slide or use a new specimen if sample is
destroyed
Stains, dust, or dirt have accumulated on the lens Clean the lens
UnclearImage There is no cover slip on the slide Add a cover slip. The objectives are designed for use
with a 0.17mm cover slip, so it is a requirement to
use one for proper images.
The cover slip is not standard sized Replace the cover slip with the appropriate 0.17mm
thickness slip
The aperture is not open to an appropriate diam-
eter
Adjust the aperture to have the light just larger than
the size of the condenser
Stain or dust has accumulated on the lens in the
inlet of the head
Clean the lens with lens cleaner or a nonabrasive
lint free cloth, as well as spray with compressed air
One side of the field
of view is dark or the
image moves while
focusing
The specimen slide is not fixed Secure the slide to the stage with clips
The nosepiece is not in the right position Turn the nosepiece until it clicks into place
The field of view is not
bright enough
The iris disc diaphragm is not big enough Rotate the iris disc diaphragm to allow more light
to travel
Stains, dust, or dirt has accumulated on the con-
denser, objective, eyepieces, or base lens
Thoroughly clean tall lenses with lens cleaner or a
lint free nonabrasive cloth

17
M620
Series
T
roubleshooting
Common Issues (Continued)
Symptom Cause Remedy
OPTICAL ISSUES
The color of the image is
notaccurate
The brightness adjustment knob is not in the right position Adjust the brightness knob to a higher or lower setting for color
clarity
No filter is used or filter is in use Remove color filter if natural light is desired, or insert desired
filter
MECHANICALISSUES
The objective touches the
cover slip
The cover slip is not standard sized Replace the cover slip with the appropriate 0.17mm thickness slip
The limit-stop is set too high or not engaged Be careful to avoid contact between objective and the slide when
the limit stop is not engaged. To reengage, focus the sample, then
lock the limit stop into place to set max height at a safe but us-
able distance.
Unable to move the slide
smoothly
The slide is not secured correctly Adjust the slide to use the stage clips and secure the sample
The mechanical stage is not properly secured Tighten the mechanical stage screws to better secure the stage
Focus knob does not turn The tension knob is too tight Loosen it by adjusting the tension ring inside the coarse focus
knob counterclockwise (close to the arm of the microscope on
the left of the microscope)
Stage declines by itself The tension knob is too lose Tighten it by adjusting the tension ring inside the coarse focus
knob clockwise (close to the arm of the microscope on the left
of the microscope)
The focusing knob won’t
raise the stage
Limit-stop is engaged Disengage the limit stop on the rear of the stage of the micro-
scope
ELECTRICAL ISSUES
The bulb/lightsource
flickers
The bulb is close to burning out Please contact us in regards to this issue. LED lights do not burn
out, so there may be another electrical issue
The microscope does not
light up
The microscope is unplugged Insert the plug into the wall socket to achieve electrical illumina-
tion