Analytik Jena SPECORD S600 User manual

Operating Manual
SPECORD S 600
UV/Vis Spectrophotometer

Analytik Jena GmbH
Konrad-Zuse-Str.1
07745 Jena Deutschland
Telefon + 49 3641 77 70
Fax + 49 3641 77 92 79
E-Mail [e-mail]
Analytik Jena GmbH
Konrad-Zuse-Str. 1
07745 Jena Deutschland
Telefon + 49 3641 77 7407
Fax + 49 3641 77 7449
E-Mail [e-mail]
http://www.analytik-jena.com
211:502.23
A (08/2021)
Analytik Jena GmbH
© Copyright 2021, Analytik Jena GmbH
Manufacturer
Service
General informationen
Documentation number
Edition
Ausführung der Techni-
schen Dokumentation

Contents
3
Contents
1Introduction................................................................................................................ 5
1.1 Intended use of the SPECORD S600................................................................................. 5
1.2 Notes on the User's Manual.............................................................................................. 5
2Safety notes................................................................................................................ 7
2.1 Warning labels on SPECORD S600................................................................................... 7
2.2 General safety regulations ................................................................................................ 7
3Technical Data of SPECORD S600 ............................................................................ 10
3.1 Physical-optical data ....................................................................................................... 10
3.2 General technical data .................................................................................................... 11
4Device description and operating principle.............................................................. 12
4.1 Mechanical design .......................................................................................................... 12
4.2 Operating principle ......................................................................................................... 13
5Transport and installation requirements................................................................. 15
5.1 Conditions for storage and transport ............................................................................. 15
5.2 Installation conditions..................................................................................................... 15
6Installation and start-up .......................................................................................... 16
6.1 Connectors and display elements ................................................................................... 16
6.2 Connecting the SPECORD S600...................................................................................... 17
6.3 Start-up ........................................................................................................................... 17
6.4 Switching off the SPECORD S600................................................................................... 19
7Accessories ............................................................................................................... 20
7.1 Standard cell holders....................................................................................................... 20
7.1.1 Universal holder .............................................................................................................. 20
7.1.2 Cell holder........................................................................................................................ 21
7.2 Further accessories.......................................................................................................... 22
8Care, maintenance, lamp change............................................................................. 24
8.1 Care and maintenance .................................................................................................... 24
8.2 Cleaning........................................................................................................................... 24
8.3 Changing lamps............................................................................................................... 25
8.3.1 Part numbers of lamps.................................................................................................... 25
8.3.2 Note on the service life of lamps .................................................................................... 25
8.3.3 Opening the flap of the lamp compartment .................................................................. 25
8.3.4 Changing the deuterium lamp........................................................................................ 27
8.3.5 Changing the halogen lamp............................................................................................ 27
8.4 Changing fuses................................................................................................................ 28
9Disposal .................................................................................................................... 30
10 List of illustrations.................................................................................................... 31

Contents
4

Introduction
5
1Introduction
1.1 Intended use of the SPECORD S600
The SPECORD S600 is a powerful, computer-controlled high-performance diode ar-
ray spectrophotometer for the measurement of the transmission, absorbance, reflec-
tion as well as the energy of liquid and solid samples. It is designed for measure-
ments in the spectral range 190 to 1020 nm.
In combination with the powerful WinASPECT software, the SPECORD S600 is par-
ticularly suitable for the use in routine laboratories with a high sample throughput,
but also provides solutions for special applications, if used in combination with the
diverse accessories available.
1.2 Notes on the User's Manual
In this manual, the following symbols are used to refer you to warnings and notes:
Warning!
Warning messages alert you to a specific procedure or practice which, if not followed
correctly,
could cause personal injury.
Danger! Hot surface!
Touching the hot surface may cause burns.
Caution!
Caution messages refer you to procedures which, if not observed, could result in dam-
age to the equipment.
Dangerous electric voltage!
Emission of UV radiation!
Disconnect power cable!
Note
T
his note must be followed to obtain correct measurement results.

Introduction
6
For easier navigation within the manual, the manual uses the following system:
Chapters and illustrations are numbered consecutively.
Every illustration has its own caption.
Steps of operation are numbered.
Cross-references are marked by an arrow (e.g. →Intended use of the SPECORD
S600 p.5)

Safety notes
7
2Safety notes
2.1 Warning labels on SPECORD S600
Observe the warning labels on the SPECORD S600!
The following warning labels have been affixed to the lamp housings of the
SPECORD S600.
Danger! UV radiation!
Do not look directly or indirectly via a mirror into the radiation emitted by the UV
lamp. The lamp radiation may cause conjunctivitis!
Danger! Hot surface!
When
lamps are switched on, their covers heat up strongly. If you intend to change
the lamps or open the lamp covers, switch the lamps off and allow for a sufficient
cool
-down time.
Extremely dangerous electrical voltage!
Several parts inside the lamp housing carry electrical voltage. Therefore, before open-
ing the lamp housing, switch off the
SPECORD S600 at the power switch and discon-
nect the power cable from the power outlet!
2.2 General safety regulations
For your personal safety and for a trouble-free operation, carefully read this chapter
before starting up the SPECORD S600.
Observe all safety notes given in this manual and all messages and notes displayed
by the control software on the screen.
In addition, observe the safety notes for system components of other manufacturers
(e.g. PC, printer, autosampler) supplied together with the device.
Intended use!
The SPECORD
S600, including original accessories, may only be used for the applica-
tions described in this manual. The manufacturer cannot assume any liability
for any
other application, including that of individual modules or single parts. This also ap-
plies to all service or repair work that is not carried out by authorized service person-
nel. All warranty claims shall be forfeited.

Safety notes
8
Local safety regulations!
Observe the local safety regulations relevant to the operation of the device (e.g. labor
-
safety regulations, regulations for the prevention of and protection against accidents).
References to possible risks given in this manual do not replace
the relevant labor-
safety regulations that are to be followed.
Personnel!
The
SPECORD S600 may only be operated by appropriately qualified and trained per-
sonnel.
For that, the knowledge of the information provided by this manual is indis-
pensable and taken for granted.
Emergency shutdown!
In the case of emergency, disconnect the
SPECORD S600 and its components from
line power by removing the power plug from the power outlet.
Electric shocks!
The
SPECORD S600 is electrically powered. At several parts inside the system, extre-
mely hazardous electrical
voltages are accessible!
To ensure Protection Class I (protective earth connection) of the device, connect the
power plug of the device only to a power outlet with protective earth conductor
. The
protective effect must not be undone by the use of an extension cable without pro-
tective earth conductor.
Before opening the device or removing any device covers, disconnect the power ca-
ble!
Before connecting the
SPECORD S600 to the power outlet, make su
re the line voltage
supplied agrees with the operating voltage specified on the rating plate at the rear
panel of the device. Operation of the device with any other operating voltage than
that specified may result in the destruction of the device.
Only use
fuses of the specified type.
Do not operate the device in explosion-risk rooms!

Safety notes
9
UV radiation!
On the
SPECORD S600
, a deuterium lamp delivers the radiation for the UV measuring
range. While measurements are running, UV radiation transmits the sample compart-
ment in the sample beam. In measurement breaks, no UV radiation is accessible
there, as the sample beam is closed by a shutter. Besides, UV radiation appears in the
lamp compartment. Before any lamp replacement, switch off the
SPECORD S600
and
disconnect the power cable!
Never look directly or indirectly, e.g. via a mirror, into the radiation emitted by the
lam
p to avoid the risk of getting conjunctivitis!
Therefore, switch off the lamp for lamp replacement!
Longer exposure of the skin to UV radiation may cause skin damage!
Lamp damage!
Do not grasp functioning halogen and deuterium lamps on their glass bulb
s. Contam-
ination on the bulb affects the radiation properties of the lamps.
Especially protect the beam
-exit window of the deuterium lamp.
Make sure to remove the protective cap from the halogen bulb after having inserted
it to avoid any risk of fire!
Non
-observance will result in damage of the lamp.
Accumulation of heat
Heat accumulation might result in overheating and faults on the device.
Make sure not to cover the ventilation slots of the
SPECORD S600!
Before you switch on the
SPECORD S600, remove its dust cover!
Handling liquids
Take care to ensure that no liquids can enter the
SPECORD S600 to avoid any dam-
age to the device.
Risk of corrosion
Do not set up the device in the vicinity of aggressive vapors, e.g. strongly corrosive
acid or caustic vapors! The vapors might corrode the connections, mechanical and op-
tical components of the device.
The SPECORD S600 was produced and tested in compliance with the following
standards and regulations:
DIN EN 61010-1 (IEC 1010-1)
73/23/EC
89/336/EC.
The SPECORD S600 meets the requirements as per Protection Type IP 20.

Technical Data of SPECORD S600
10
3Technical Data of SPECORD S600
3.1 Physical-optical data
Optical principle High-performance diode array for UV NIR
range
Optics Polychromator with concave holographic
grating and photodiode array (1024 pix-
els)
Wavelength range 190-1100 nm ±2 nm
Photometric measuring range -3 to 3 A
Photometric display range -4 to 4 A
Wavelength accuracy
(with holmium oxide filter)*
±0.5 nm
Wavelength accuracy
(D2E-peaks at 486,0 nm and 656,1 nm)
±0.3 nm
Wavelength reproducibility
(with holmium oxide filter)*
±0.02 nm
Photometric accuracy
VIS at 546nm with Hellma F4 neutral-density
filter
±0.005 A
Photometric accuracy
UV with potassium dichromate acc. to Ph.Eur.
±0.012 A
Stray light 198 nm
( KCl Merck 1.08164.0001)
≤1.0 %T
Stray light 220 nm (NaI) ≤0.05 %T
Stray light 340 nm (NaNO2) ≤0.05 %T
Long-term stability at 500 nm ≤±0.002 A/h
UV resolution (Toluene hexane) ≥1.6
Minimum measuring time 12 ms for one spectrum
* allowing for tolerances of used standard

Technical Data of SPECORD S600
11
3.2 General technical data
Weight 11 kg
Footprint (W x D) approx. 900x750 mm2with PC
Dimensions (W x H x D) 680 x 190 x 250 mm3
Line voltage 230 VAC ±10 % or
115 VAC ±10 % (switchable by service)
Frequency 47 ... 63 Hz
Power consumption 140 VA
Fuses:
Line fuses
230 VAC: T 3.15 AH/250V, Type 19181 Wick-
mann
115 VAC: T 3.15 AH/250V, Type 19181 Wick-
mann
Radio noise suppression EN 61326 Class B
Noise immunity EN 61326
Protection Type IP 20
Data interfaces 2 x RS-232 for accessories
1 x USB port for the connection of an external
PC
1 x RS–232 for the connection of an external PC
PC requirements for software WinAS-
PECT
Pentium processor 133 MHz, 16 MB RAM,
Windows 2000 or Windows XP

Device description and operating principle
12
4Device description and operating principle
4.1 Mechanical design
The appearance of the SPECORD S600 is marked by the lamp compartment on the
right side, the central sample compartment that is open to three sides, and the poly-
chromator section on the left side.
1
Lamp compartment
2
Sample compartment with carrier rails for holding accessories
3
Polychromator
Fig. 1 Mechanical design of SPECORD S600
Unlike scanning spectrophotometers with monochromator, the optical design of the
SPECORD S600 with its polychromator requires a few moving components only and
is mechanically very stable.
Fig. 2 Optical path of SPECORD S600

Device description and operating principle
13
Light sources
The light sources used are a halogen lamp for the VIS and in-
frared region and a deuterium lamp for the UV region.
The lamp-selector slider serves to couple the light of either the
halogen or the deuterium lamp, or the light of both lamps sim-
ultaneously into the optical path.
In measurement breaks, lamp radiation is shut off by shutters.
That way, it is possible to perform dark-current measurements
and protect the sample from unnecessary radiation exposure.
Sample compart-
ment
The optical setup with polychromator allows an open sample
compartment. The bundle of rays is restricted by optical slits to
the lamp compartment and the polychromator side each. The
accessory units are clamped to the carrier rails of the sample
compartment.
Polychromator
The polychromator used is the patented MCS module (Multi
Channel System) from Carl Zeiss. The entrance slit, the imag-
ing holographic grating and the detector are embedded in a
ceramic housing.
4.2 Operating principle
The undispersed light of the halogen and/or the deuterium lamp transmits the sam-
ple and is partly absorbed by the sample. The thus altered light is spectrally dispersed
in the polychromator by the holographic grating. The produced spectrum is imaged
onto the diode array. The measured values of the individual photodiodes thus repre-
sent the value of intensity Iover a small spectral region. As this is done in a very fast
process, this type of measurement is called simultaneous.
However, reading the individual photodiodes takes a certain time (integration time).
This integration time can be varied within predefined limits. The shortest integration
time possible depends, inter alia, on the number of photodiodes per line. A compro-
mise has to be made in selecting the most favorable integration time. A smaller inte-
gration time means, that there is less time for the photodiode to capture the light to
be measured. The level of the measured signal will be lower and thus the signal-to-
noise ratio deteriorate. On the other hand, a too high integration time may result in
an intensity that exceeds the level the photodiode can receive (degree of saturation).
The corresponding sections of the diode array are overdriven and the found trans-
mission or absorbance values are wrong. However, a high integration time may be
quite desirable if measurements shall be taken in low-energy regions of the spec-
trum (e.g. 900 – 1020 nm) and the overdriven areas are deliberately eliminated for
the analysis. The system provides software-supported automatic search of integra-
tion times suitable for the analytical measuring range.

Device description and operating principle
14
In absorption measurements, the incident intensity IR (reference value) and the in-
tensity ISattenuated by the sample is measured at every wavelength of interest.
Measurement is in arbitrary units, as only the ratio of both values is of importance.
Quantitatively, the absorption behavior is expressed in transmission T[%] or absorp-
tion A:
However, also the dark current must be considered for result generation. The dark
current is the intensity IDof the ambient light that may enter the polychromator
through the open sample compartment including the currents on the diode array
caused by electronics. The intensity of the dark current must be subtracted from the
intensities of sample and reference. The dark current can be measured automatically
or – in very time-critical measurements – started manually. The corresponding op-
tions are to be selected along with other measurement parameters in WinASPECT®
software.
Transmission and absorption are calculated according to the following formulas:
DR
DS
II
II
D−
−
=
D Transmission
IRUnattenuated intensity (reference value)
ISIntensity after attenuation by the sample
ID Dark current
T[%] Percent transmission
A Absorption
100DT[%] ∗=
Dlg
A−=

Transport and installation requirements
15
5Transport and installation requirements
5.1 Conditions for storage and transport
For transport and storage, the following ambient conditions must be complied with:
Temperature range in transport: -40...+70 °C
Relative humidity in transport: up to 95 %
5.2 Installation conditions
Caution!
Do not cover the ventilation slots of the device!
Do not place any objects on the device!
Do not operate the device in explosion
-risk rooms!
Keep aggressive vapors, e.g. strongly corrosive acid or caustic vapors away from
the
vicinity of the device.
The installation site of the SPECORD S600 must meet the following requirements:
The place should be free of draft, dust, corrosive vapors as well as vibrations.
Do not set up the SPECORD S600 near electromagnetic fields (e.g. of motors).
Avoid dripping or splashing water near the instrument.
Do not expose the device to direct sunlight or the radiation of heaters.
Temperature range: + 15 ... + 35 °C
Humidity: up to 90 % (at + 30 °C)
Considering the dimensions of the SPECORD S600 and a clearance of approximately
100 mm to the sides and the front, the device needs a bench space of 880 x 450
mm2.

Installation and start-up
16
6Installation and start-up
6.1 Connectors and display elements
The connectors and display elements of the SPECORD S600 are arranged on its front
and back wall for easy access.
The LED indicators on the left front side of the SPECORD S600 serve to indicate line
power and lamp operation.
1
Halogen lamp ON LED (red)
2
D
2
E lamp ON LED (yellow)
3
Line power ON LED (green)
Fig. 3 Display elements on front panel
The LED's light when the SPECORD S600 has been switched on and the lamps are
on.
The power-input connector with fuse holder, the power switch, and the connectors
for PC and accessories are arranged on the back panel of the device.
1
Connector for accessories
5
Type label
2
Connector for accessories
6
Power switch
3
RS232 port for PC
7
Fuse holder for line fuse
4
USB port for PC
8
Power input connector
Fig. 4 Connectors on back panel
12345678

Installation and start-up
17
6.2 Connecting the SPECORD S600
Observe correct position of device!
Move the
SPECORD S600 only in upright position (observe label on transport case).
Do not turn the device upside down.
Electric shock
Bef
ore connecting the SPECORD S600 and the PC to line power, make sure the avail-
able line voltage agrees with the operating voltage specified on the type labels. Oper-
ation of the devices on line voltages other than the specified operating voltage may
result in th
eir destruction.
1. Remove the device from its transport packaging and remove the plastic cover.
Wait until the device has adjusted to the ambient temperature.
2. Install the software on the PC (→Refer to WinASPECT®User's Manual).
3. Connect the SPECORD S600 (→see Connectors and display elements p.16) to
the PC (free COM port or USB port) using the supplied connecting cable.
Note
When you
use the autosampler, you must connect the PC to the USB port and the au-
tosampler to the serial port!
4. Connect one power cable each to the SPECORD S600 and the PC. Connect the
power cables of the SPECORD S600 and the PC to power outlets that are con-
nected to the same phase of the line power supply (multiple socket outlet, if pos-
sible).
6.3 Start-up
Do not cover ventilation slots!
Take care to ensure that the ventilation slots on the top and on the right and left bot-
tom of the
SPECORD S600 are not covered. Do not place any objects in front of the
openings. The suction intakes for the two fans on the bottom of the device must not
be covered by light objects placed on the tabletop (e.g. a loose sheet of paper.
Connecting SPECORD
S600 and PC

Installation and start-up
18
1. Start WinASPECT®software by a click on the program icon on the Windows desk-
top or by clicking the Start button on the taskbar:
Start / Programs / WinASPECT / WinASPECT
Fig. 5 WinASPECT®program icon
2. Switch on the SPECORD S600 at the power switch on the back panel of the de-
vice (→Fig. 4 p. 16).
The green "Power ON" LED on the front panel of the device starts lighting (→see
Fig. 3, Display elements on front panel, p.16).
3. In WinASPECT®software, select the SPECORD S600 – PC port: used.
Activate the Measurement / Set Parameters menu function to open the window
of the SPECORD S600 device driver.
Fig. 6 Dialog box of SPECORD S600 device driver
Click on the General tab.
Choose the port of the SPECORD S600, to which the PC has been connected.
Close the device driver window by a click on the [OK] button.
4. Initialize the device to establish the communication between PC and SPECORD
S600. For that, activate the Measurement / Initialize Device menu command.
The SPECORD S600 is ready now for taking measurements.
Note

Installation and start-up
19
Consider that the SPECORD S600 needs a warm-up time of one hour to reach ther-
mal stability. Therefore,
do not start high-precision measurements unless the warm-
up period is over.
6.4 Switching off the SPECORD S600
1. Exit the WinASPECT®session using the File / Exit menu command.
2. The shut down the computer.
3. Finally switch off the SPECORD S600 at the power switch.
Note
If you have finished the measurements on the
SPECORD S600, and you want to ana-
lyze the results on the PC, you may also switch off the
SPECORD S600 without shut-
ting down the computer before
.

Accessories
20
7Accessories
7.1 Standard cell holders
The standard scope of supply of the SPECORD S600 includes a universal holder and a
cell holder for cells of up to 50 mm pathlength.
7.1.1 Universal holder
1
Mount for cell holders or solid sample holders
2
Clamping lever
Fig. 7 Universal holder
Fig. 8 Installing and clamping the universal holder
1. Put the universal holder with its hook onto the rear carrier rail and push it close
to the right sample compartment wall.
2. Throw the clamping lever to the right.
Note
Inserting the universal
holder in the sample com-
partment