Analytik Jena SPEKOL 1300 User manual

SPEKOL®1300
UV VIS Spectrophotometer

Analytik Jena AG
Kundendienst
Konrad-Zuse-Str. 1
07745 Jena
Phone: Hotline: + 49 (3641) 77-7407
Fax: + 49 (3641) 77-7449
e-mail: [email protected]
General information about Analytik Jena AG
on the internet: http://www.analytik-jena.de
Copyrights and Trademarks
Microsoft, Windows 2000 / XP / Vista, MS Excel are registered trademarks of Microsoft Corp.
Publication No.: 210:302.23
Edition – July 2008
Technical documentation made by:
Analytik Jena AG
This documentation describes the state of this product at the time of publishing. It
need not necessarily agree with future versions of the product.
Subject to change!
© Copyright 2008 Analytik Jena AG

Contents
Contents
1Introduction..............................................................................3
1.1 Warning and safety labels on SPEKOL 1300...................................................3
1.2 Notes on the User's Manual .............................................................................4
2Safety notes..............................................................................5
3Technical data..........................................................................7
4Design of the SPEKOL 1300 ...................................................9
5Unpacking instruction...........................................................11
5.1 Installation and transport conditions ...............................................................11
5.2 Installation and start-up ..................................................................................11
5.3 Select light source ..........................................................................................12
5.4 Turn the Lamps on/off ....................................................................................12
6Maintenance ...........................................................................13
6.1 Cleaning .........................................................................................................13
6.2 Replacing fuses..............................................................................................13
6.3 Replacing Halogen lamp ................................................................................14
6.4 Replacing Deuterium lamp .............................................................................16
7Accessories............................................................................17
7.1 Manual 4 cell changer ....................................................................................17
7.2 Adjustable cell holder .....................................................................................17
7.3 Round cell holder ...........................................................................................18
7.4 Extension of sample compartment .................................................................18
7.5 Flow-through unit............................................................................................19
7.6 Thermostatted cell holder...............................................................................19
7.7 Thermostatted 3 cell manual changer ............................................................19
7.8 Printer.............................................................................................................20
8Display and Buttons of SPEKOL 1300.................................21
9Software function...................................................................23
9.1 Special function..............................................................................................23
9.2 Select Language Mode ..................................................................................24
9.3 UV / VIS lamp on / off.....................................................................................24
9.4 Absorbance / Transmittance Measurement....................................................24
9.5 Concentration mode .......................................................................................25
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Contents
9.5.1 Determination of the concentration via one point calibration ..........................25
9.5.2 Determination of the concentration via factor .................................................25
9.5.3 Determination of the concentration via calibration curve ................................26
9.6 DNA / Protein Analytics ..................................................................................28
9.7 Time Scan ......................................................................................................29
10 Disposal..................................................................................31
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Introduction
1 Introduction
The SPEKOL®1300 is a single beam spectrophotometer. The instrument is compact,
easy-to-use, and ruggedness. The large sample compartment accept various
accessories and cell from 10mm to 100mm path length. The built-in RS-232 serial
interface enables the instrument to be connected to a printer.
The SPEKOL®1300 is ideal to use in a clinical laboratory, biochemical laboratory, petrol
chemical laboratory, environmental protection and various fields of quality control.
This user′s manual provides the description of the SPEKOL®1300, a list of main
specifications and available accessories.
After carefully unpacking the content, check the materials against the packing list to
ensure that you have received everything in good condition. If some parts are missing,
damaged or have any other defects, please contact you dealer or the sales
representative immediately.
1.1 Warning and safety labels on SPEKOL 1300
The following warning and safety labels are located on the SPEKOL®1300:
Caution! Hazardous area. Do not remove this cover under any circumstances. Trained
service peoples only. No serviceable components inside. Select the right voltage.
Disconnect the power cable before opening the device cover or replacing fuses!
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Introduction
1.2 Notes on the User's Manual
In this manual, the following symbols are used to refer you to warnings and notes:
Warning!
Warning messages alert you to a specific procedure or practice which, if not followed
correctly, could cause personal injury.
Caution!
Caution messages refer you to procedures which, if not observed, could result in
damage to the equipment.
Caution! Dangerous electric voltage!
Caution! Emission of UV radiation!
Disconnect power cable!
Note
This note must be followed to obtain correct measurement results.
For easier navigation within the manual, the manual uses the following system:
• Chapters and illustrations are numbered consecutively.
• Every illustration has its own caption.
• Steps of operation are numbered.
• Cross-references are marked by an arrow (e.g. →Notes on the User's
Manual p.3)
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Safety notes
2 Safety notes
For your personal safety and for a trouble-free operation, carefully read this chapter
before starting up the SPECKOL®1300.
Observe all safety notes given in this manual and all messages and notes displayed by
the control software on the screen.
In addition, observe the safety notes for system components of other manufacturers
(e.g. PC, printer) supplied together with the device.
In particular, you should also observe the safety notes given on the labels and the
information on the use, storage and disposal of reagents, reaction cells and the notes on
the package inserts of test kits.
Intended use!
The SPEKOL®1300, including original accessories, may only be used for the
applications described in this manual. The manufacturer cannot assume any liability for
any other application, including that of individual modules or single parts. This also
applies to all service or repair work that is not carried out by authorized service
personnel. All warranty claims shall be forfeited.
Local safety regulations!
Observe the local safety regulations relevant to the operation of the device (e.g. labor-
safety regulations, regulations for the prevention of and protection against accidents).
References to possible risks given in this manual do not replace the relevant labor-safety
regulations that are to be followed.
Personnel!
The SPEKOL®1300 may only be operated by appropriately qualified and trained
personnel.
For that, the knowledge of the information provided by this manual is indispensable and
taken for granted.
Emergency stop!
In the case of emergency, disconnect the SPEKOL®1300 and its components from line
power by removing the power plug from the power outlet.
Caution! This procedure involves the risk of data loss and damage to the operating
system!
Electric shocks!
The SPEKOL®1300 is electrically powered. At several parts inside the system,
extremely hazardous electrical voltages are accessible!
To ensure Protection Class I (protective earth connection) of the device, connect the
power plug of the device only to a power outlet with protective earth conductor. The
protective effect must not be undone by the use of an extension cable without protective
earth conductor.
Before opening the device or removing any device covers, disconnect the power cable!
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Safety notes
Before connecting the SPEKOL®1300 to the power outlet, make sure the line voltage
supplied agrees with the operating voltage specified on the rating plate at the rear panel
of the device. Operation of the device with any other operating voltage than that
specified may result in the destruction of the device.
Only use fuses of the specified type.
Do not operate the device in explosion-risk rooms!
UV radiation!
Never look directly or indirectly, e.g. via a mirror, into the radiation emitted by the lamp to
avoid the risk of getting conjunctivitis!
Therefore, switch off the lamp for lamp replacement!
Lamp damage!
Do not grasp functioning halogen and deuterium lamps on their glass bulbs.
Contamination on the bulb affects the radiation properties of the lamps.
Especially protect the beam-exit window of the deuterium lamp.
Accumulation of heat
Heat accumulation might result in overheating and faults on the device.
Make sure not to cover the ventilation slots of the SPEKOL®1300!
Before you switch on the SPEKOL®1300, remove its dust cover!
Handling liquids
Take care to ensure that no liquids can enter the SPEKOL®1300 to avoid any damage
to the device.
Risk of corrosion
Do not set up the device in the vicinity of aggressive vapors, e.g. strongly corrosive acid
or caustic vapors! The vapors might corrode the connections, mechanical and optical
components of the device.
Avoid exposing the sample compartment to strongly corrosive substances! For the
analysis of such samples, use vapor-tight stoppered cells.
After the measurement, do not leave the samples unnecessarily long in the sample
compartment of the device!
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Technical data
3 Technical data
Model SPEKOL®1300
Optical System Littrow type, single beam, grating system 1200 line/mm
Spectral Slit width 4 nm
Wavelength Range 190 to 1100 nm
Wavelength Accuracy ±2 nm
Repeatability 1 nm
Stray Radiant Energy ≤0.3 %T at 220 nm and 340 nm
Photometric Range
0 %T to 125.0 %T
-0.1 A to 3 A
0 C to 9999 C
Stability ±0.002 A/hr (at 500 nm after 1 hour warm up)
Photometric Accuracy ±0.01 A
Power Requirement adjustable 115 / 230 VAC, ±10%
60/50 Hz
Line fuses 2 x T 4 AH
Temperature working range 15°C – 35°C
Humidity working range 85 %
Size (W x D x H) 465 mm (18.3’) ×365 mm (14.3’) ×175 mm (6.9’)
Weight 11.5 kg
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Technical data
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Design of the SPEKOL 1300
4 Design of the SPEKOL 1300
1 Display and Button display of data’s and control buttons for use of the internal software
function
2 Sample compartment to insert accessories and sample cells
3 Cell selector arm of the Cell changer to change the position of the manual cell changer
Fig. 1 SPEKOL®1300 front side
Note
Pay attention that the cover of the sample compartment must be closed during the
measurements.
4 RS 232 Interface connection of printer
5 Analog output
Fig. 2 SPEKOL®1300 right side
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Design of the SPEKOL 1300
6 Power cable socket
7 Printer power connection power cable socket from the printer
8 Fuse holder
9 Voltage selection to select the voltage between 220 and 110 V
10 Power switch
Fig. 3 SPEKOL®1300 back side
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Unpacking instruction
Installation and transport conditions
5 Unpacking instruction
5.1 Installation and transport conditions
Installation conditions
Considering the dimensions of the SPEKOL ®and a clearance of approximately 100 mm
to the sides and the front, the device needs a bench space of at least 665 x 565 mm.
Caution!
Do not cover the ventilation slots of the device!
Do not place any objects on the device!
Do not operate the device in explosion-risk rooms!
Keep aggressive vapors, e.g. strongly corrosive acid or caustic vapors away from the
vicinity of the device.
The installation site of the SPECORD®must meet the following requirements:
• The place should be free of draft, dust, corrosive vapors as well as
vibrations.
• Do not set up the SPECORD®near electromagnetic fields (e.g. of motors).
• Avoid dripping or splashing water near the instrument.
• Do not expose the device to direct sunlight or the radiation of heaters.
• Temperature range: + 15 ... + 35 °C
• Humidity: up to 85 % (at + 30 °C)
Conditions for storage and transport
For transport and storage, the following ambient conditions must be complied with:
• Temperature range in transport: -40...+70 °C
• Relative humidity in transport: up to 95 %
5.2 Installation and start-up
Observe correct position of device!
Move the SPECORD®only in upright position (observe label on transport case).
Do not turn the device upside down.
Electric shock
Before connecting the SPECORD®and the PC to line power, make sure the available
line voltage agrees with the operating voltage specified on the type labels. Operation
of the devices on line voltages other than the specified operating voltage may result in
their destruction.
1. Unpack the contents carefully and check the materials.
2. Place the instruments in suitable location.
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Unpacking instruction
Select light source
3. Remove any obstructions or materials that could hinder the flow of air under and
around the instrument vibration.
4. Select the right voltage.
5. Plug the power cord into an outlet. Turn on the instrument and allow it to warm up
for 20 minutes before taking any readings. A self test starts automatically.
Your spectrophotometer is now ready for operation.
5.3 Select light source
You may use the [FUNC] key to selecting an available lamp for measurements
depending on your samples. The light sources can be exchanged between 325nm to
355nm.
1. Press the [FUNC] button until display shows “LAMP EXCHANGE?”
2. Press up [ ∧]or [∨]button to set the wavelength point of lamp exchanging
(proposal at 325 nm).
3. Press [ENT] to store the parameter to be entered.
5.4 Turn the Lamps on/off
Depending on the analytical method you can switch the halogen lamp and the deuterium
lamp on or off.
1. Press [FUNC] until display shows “VIS lamp on?” (“ VIS lamp off?” ), then press
the [ENT] button to turn on or off the lamp.
2. Press [FUNC] until display shows “UV lamp on?” ( “UV lamp off?” ), then press
the [ENT] button to turn on or off the lamps.
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Maintenance
Cleaning
6 Maintenance
This SPEKOL®1300 spectrophotometer has been designed to be durable and reliable.
Therefore, the routine maintenance of the instrument is minimal.
However, you will need to replace the lamp or you may want check the wavelength
calibration and photometric linearity to verify the instruments′s performance.
The manufacturer recommends only authorized service representatives to perform
procedures requiring removal of the instrument′s cover and replacement of electrical
component′s. To protect both of yourself and your instrument, be sure to contact the
authorized service representative to perform any service procedure, when you do not
feel comfortable performing.
6.1 Cleaning
If sample substance was spilled in the sample compartment or accessory, instantly wipe
it up using tissue paper.
For cleaning the device cover from dust or spillings, please use a soft cloth and a
commercial neutral cleanser.
Do not expose SPEKOL®1300 to corrosive atmosphere.
6.2 Replacing fuses
If the line fuses are defective, you can change them yourself.
Risk of electric shock!
Before changing the fuses, make sure to switch the SPEKOL®1300 off with the
power switch and disconnect the power cable from the power input connector of the
device.
1. Remove the power cable from the SPEKOL®1300.
2. Open the bayonet connections of the fuse holders (→Fig. 3 p.10) by turning to the
left using a screwdriver. The fuseholder jumps up a little.
3. Replace the defective line fuses.
Make sure to use only the following fuse types:
• T 4 AH.
4. Re-insert fuse holder and lock the bayonet connection.
5. Reconnect the power cable to the power input connector of the SPEKOL®1300
and the power outlet.
6. Switch the SPEKOL®1300 on again.
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Maintenance
Replacing Halogen lamp
6.3 Replacing Halogen lamp
Electric shock!
Before changing the halogen lamp, make sure to switch the device off with the power
switch.
Caution: Risk of burns!
Allow the halogen lamp to cool down to avoid the risk of burns!
Caution: Eye injury!
Never look into the lighting lamp as the radiation may cause eye injury!
Caution!
Do not touch the glass bulb of the lamp to avoid any contamination affecting its
performance!
1. Turn the power off and unplug the instrument.
2. Put all samples and not fixed elements out of the sample compartment.
3. Place the SPEKOL 1300 carefully on a soft underlay on the top side of the device
so that you can access the underside.
Fig. 4 Lamp house cover on the under side of SPEKOL ®
4. Remove the two screws (11) and the lamp house cover (12) on the underside of the
instrument.
Fig. 5 Lamp compartment of SPEKOL®, opened
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Maintenance
Replacing Halogen lamp
5. Unscrew the holder of the halogen lamp (14) and take the holder with the halogen
lamp out of the device.
Fig. 6 SPEKOL®1300holder of the halogen lamp
6. Remover the lamp (16) from lamp holder
7. Replace the new lamp carefully in the holder.
Caution
Do not handle Halogen lamp with bare fingers. Use a piece of tissue or cloth when handling the
lamp. The oil from your fingers can cause the lamp to burn out prematurely!
8. Replace and fix the holder with the new halogen lamp in the device
9. Close the cover of the lamp house using the 2 screws.
10. Replace the device with the top side on top.
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Maintenance
Replacing Deuterium lamp
6.4 Replacing Deuterium lamp
Risk of electric shock!
Before changing the deuterium lamp, make sure to switch the device off with the power
switch.
Caution: Risk of burns!
Allow the deuterium lamp to cool down to avoid the risk of burns!
Emitted UV radiation!
Never look directly or indirectly, e.g. via a mirror, into the radiation emitted by the lamp
to avoid the risk of getting conjunctivitis!
Dirt on beam exit window!
Do not touch the glass bulb of the new deuterium lamp. Particularly avoid contamination
on the quartz glass beam exit window! If you touched the glass bulb with your fingers,
wipe the fingerprints off with a clean, lintfree cloth and pure alcohol. The performance of
the lamp will deteriorate by contamination.
1. Turn off the power and unplug the instrument
2. Put all samples and not fixed elements out of the sample compartment
3. Place the SPEKOL 1300 carefully on a soft underlay on the top side of the device
so that you can access the underside
4. Remove the two screws (11) and the lamp house cover (12) on the underside of the
instrument
5. Unscrew the 2 screws of the deuterium lamp (13), disconnect the electronic plug
connection (15) and take the deuterium lamp out of the device
Do not handle Deuterium lamp with bare fingers. Use a piece of tissue or cloth when
handling the lamp. The oil from your fingers can cause the lamp to burn out prematurely!
6. Replace the new deuterium lamp and fix it with the screw
7. Close the cover of the lamp house using the 2 screws.
8. Replace the device with the top side on top.
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Accessories
7 Accessories
For the installation of accessory use the two screws on the bottom of the sample
compartment.
7.1 Manual 4 cell changer
The standard configuration is a 4 cell changer for 1 cm cells. There are also 4 cell
changer for 5 and 10 cm cell available. As an option there are also 4 cell changer for
cells with 50 and 100 mm path length available.
Fig. 7 Manual 4 cell changer
Use the cell selector arm (→Fig. 1, p.9) of the manual cell changer to set the position of
the manual cell changer.
Position 5
Position 4
Position 3
Position 2
Position 1
0%T
Sample 1
Sample 2
Sample 3
Reference
Fig. 8 Positions of the 4 cell changer
7.2 Adjustable cell holder
Micro cells and ultra micro cells have the standard outside width of 12.5 mm, but a
reduced inside width or filling aperture of 1 to 4 mm. This requires accurate alignment of
the cell in the light beam.
This adjustable cell holder can be used for cells with an aperture high of 8,5 mm.
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Accessories
Fig. 9 SPEKOL®1300 adjustable cell holder
1. Insert the adjustable cell holder in the sample compartment with screw 1 to the
back site.
2. Use the screw 1 to adjust the cell horizontal to the light beam and screw 2 to adjust
the cell vertical to the light beam.
The cell has the optimal position if the highest transmittance is reached.
7.3 Round cell holder
This special cell holder can be used for round cell of standard test kits. Cells with a
diameter up to 16 mm and a high up to 11 cm can be used.
7.4 Extension of sample compartment
This extension is an attachment for tube openings that allows the use of thermostatted
cell holders. It can easily be adapted to the SPEKOL®1500 sample compartment.
Fig. 10 SPEKOL®1500 extension of sample compartment
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