Analytik Jena SPEKOL 1500 User manual

SPEKOL®1500
UV VIS Spectrophotometer

Analytik Jena AG
Kundendienst
Konrad-Zuse-Str. 1
07745 Jena
Phone: Hotline: + 49 (3641) 77-7407
Fax: + 49 (3641) 77-7449
e-mail: [email protected]
General information about Analytik Jena AG
on the internet: http://www.analytik-jena.de
Copyrights and Trademarks
Microsoft, Windows 2000 / XP / Vista, MS Excel are registered trademarks of Microsoft Corp.
Edition – July 2008
Technical documentation made by:
Analytik Jena AG
This documentation describes the state of this product at the time of
publishing. It need not necessarily agree with future versions of the product.
Subject to change!
© Copyright 2008 Analytik Jena AG

Contents
Contents
1Introduction ..............................................................................3
1.1 Warning and safety labels on SPEKOL 1500...................................................3
1.2 Notes on the User's Manual .............................................................................4
2Safety notes..............................................................................5
3Technical data ..........................................................................7
4Unpacking instruction .............................................................9
4.1 Installation and transport conditions .................................................................9
4.2 Unpacking instruction .....................................................................................10
5Design of the SPEKOL 1500 .................................................11
6Maintenance ...........................................................................13
6.1 Cleaning .........................................................................................................13
6.2 Replacing fuses..............................................................................................13
6.3 Replacing Halogen lamp ................................................................................14
6.4 Replacing Deuterium lamp .............................................................................15
7Accessory...............................................................................17
7.1 Manual 4 cell changer ....................................................................................17
7.2 Adjustable cell holder .....................................................................................17
7.3 Round cell holder ...........................................................................................18
7.4 Extension of sample compartment .................................................................18
7.5 Flow-through unit............................................................................................19
7.6 Thermostatted cell holder ...............................................................................19
7.7 Thermostatted 3 cell manual changer ............................................................19
7.8 Printer.............................................................................................................20
8Display and Buttons of SPEKOL 1500 .................................21
9Software function................................................................... 23
9.1 Photometric mode ..........................................................................................23
9.2 Quantitative measurement .............................................................................24
9.3 Wavelength scan............................................................................................26
9.4 Kinetic Analysis ..............................................................................................27
9.5 Multi- Wavelength Measurement....................................................................28
9.6 DNA/ Protein measurement ...........................................................................28
9.7 System setup .................................................................................................29
User’s manual SPEKOL®1500 Edition 07/2008 1
Analytik Jena AG

Contents
9.8 System positioning .........................................................................................30
9.9 Lights and A/D Conversion.............................................................................30
9.10 Connect to PC................................................................................................31
10 Disposal ..................................................................................33
2 Edition 07/2008 User’s manual SPEKOL®1500
Analytik Jena AG

Introduction
1 Introduction
The SPEKOL®1500 is a single beam spectrophotometer. The instrument is compact,
easy-to-use, and rugged. The large sample compartment accepts various accessories
and cell from 10mm to 100mm path length. The built-in RS-232 serial interface enables
the instrument to be connected to a printer.
The SPEKOL®1500 is ideal to use in a clinical laboratory, biochemical laboratory, petrol
chemical laboratory, environmental protection and various fields of quality control.
This users manual provides the description of the SPEKOL®1500, a list of main
specifications and available accessories.
After carefully unpacking the content, check the materials against the packing list to
ensure that you have received everything in good condition. If some parts are missing,
damaged or have any other defects, please contact your dealer or the sales
representative immediately.
1.1 Warning and safety labels on SPEKOL 1500
The following warning and safety labels are located on the SPEKOL®1500:
Caution! Hazardous area. Do not remove this cover under any circumstances. Trained
service people only. No serviceable components inside. Select the right voltage.
Disconnect the power cable before opening the device cover or replacing fuses!
User’s manual SPEKOL®1500 Edition 07/2008 3
Analytik Jena AG

Introduction
1.2 Notes on the User's Manual
In this manual, the following symbols are used to refer you to warnings and notes:
Warning!
Warning messages alert you to a specific procedure or practice which, if not followed
correctly, could cause personal injury.
Caution!
Caution messages refer you to procedures which, if not observed, could result in
damage to the equipment.
Caution! Dangerous electric voltage!
Caution! Emission of UV radiation!
Disconnect power cable!
Note
This note must be followed to obtain correct measurement results.
The following symbols are used:
Cross-reference: Refers to another section/illustration of the manual.
For easier navigation within the manual, the manual uses the following system:
 Chapters and illustrations are numbered consecutively.
 Every illustration has its own caption.
 Steps of operation are numbered.
 Cross-references are marked by an arrow (e.g. Notes on the User's
Manual p.3)
4 Edition 07/2008 User’s manual SPEKOL®1500
Analytik Jena AG

Safety notes
2 Safety notes
For your personal safety and for a trouble-free operation, carefully read this chapter
before starting the SPEKOL®1500.
Observe all safety notes given in this manual and all messages and notes displayed by
the control software on the screen.
In addition, observe the safety notes for system components of other manufacturers
(e.g. PC, printer) supplied together with the device.
In particular, you should also observe the safety notes given on the labels and the
information on the use, storage and disposal of reagents, reaction cells and the notes on
the package inserts of test kits.
Intended use!
The SPEKOL®1500, including original accessories, may only be used for the
applications described in this manual. The manufacturer cannot assume any liability for
any other application, including that of individual modules or single parts. This also
applies to all service or repair work that is not carried out by authorized service
personnel. All warranty claims shall be forfeited.
Local safety regulations!
Observe the local safety regulations relevant to the operation of the device (e.g. labor-
safety regulations, regulations for the prevention of and protection against accidents).
References to possible risks given in this manual do not replace the relevant labor-safety
regulations that are to be followed.
Personnel!
The SPEKOL®1500 may only be operated by appropriately qualified and trained
personnel.
For that, the knowledge of the information provided by this manual is indispensable and
taken for granted.
Emergency stop!
In the case of emergency, disconnect the SPEKOL®1500 and its components from line
power by removing the power plug from the power outlet.
Caution! This procedure involves the risk of data loss and damage to the operating
system!
Electric shocks!
The SPEKOL®1500 is electrically powered. At several parts inside the system,
extremely hazardous electrical voltages are accessible!
To ensure Protection Class I (protective earth connection) of the device, connect the
power plug of the device only to a power outlet with protective earth conductor. The
protective effect must not be undone by the use of an extension cable without protective
earth conductor.
Before opening the device or removing any device covers, disconnect the power cable!
User’s manual SPEKOL®1500 Edition 07/2008 5
Analytik Jena AG

Safety notes
Before connecting the SPEKOL®1500 to the power outlet, make sure the line voltage
supplied agrees with the operating voltage specified on the rating plate at the rear panel
of the device. Operation of the device with any other operating voltage than that
specified may result in the destruction of the device.
Only use fuses of the specified type.
Do not operate the device in explosion-risk rooms!
UV radiation!
Never look directly or indirectly, e.g. via a mirror, into the radiation emitted by the lamp to
avoid the risk of getting conjunctivitis!
Therefore, switch off the lamp for lamp replacement!
Lamp damage!
Do not grasp functioning halogen and deuterium lamps on their glass bulbs.
Contamination on the bulb affects the radiation properties of the lamps.
Especially protect the beam-exit window of the deuterium lamp.
Risk of burns
The lamp housing will heat up when lamps are operating. Contact with hot surfaces may
cause burns. Before exchanging a lamp, please switch of the SPEKOL®1500, unplug the
divice and wait until the lamps cooled down.
Accumulation of heat
Heat accumulation might result in overheating and faults on the device.
Make sure not to cover the ventilation slots of the SPEKOL®1500!
Before you switch on the SPEKOL®1500, remove its dust cover!
Handling liquids
Take care to ensure that no liquids can enter the SPEKOL®1500 to avoid any damage
to the device.
Risk of corrosion
Do not set up the device in the vicinity of aggressive vapors, e.g. strongly corrosive acid
or caustic vapors! The vapors might corrode the connections, mechanical and optical
components of the device.
Avoid exposing the sample compartment to strongly corrosive substances! For the
analysis of such samples, use vapor-tight stoppered cells.
After the measurement, do not leave the samples unnecessarily long in the sample
compartment of the device!
6 Edition 07/2008 User’s manual SPEKOL®1500
Analytik Jena AG

Technical data
3 Technical data
Model SPEKOL®1500
Optical System Littrow type, single beam, grating system 1200 Line/mm
Spectral Slit width 2 nm
Wavelength Range 190 to 1100 nm
Wavelength Accuracy 2 nm
Repeatability 1 nm
Stray Radiant Energy ≤0.3 %T at 220 nm and 340nm
Photometric Range
0 %T to 125.0 %T
-0,1 A to 3 A
0 C to 9999 C
Stability 0.002 A/hr (at 500 nm after 1 hour warm up)
Photometric Accuracy 0.01 A
Power Requirement 115 V / 230 V (adjustable)
Frequency 50 to 60Hz
Line fuses 2x T 4 AH
Temperature working range +15°C to +35°C
Humidity working range < 85%
Size (W x D x H) 465 mm (18.3’) 365 mm (14.3’) 175 mm (6.9’)
Weight 11.5 kg
User’s manual SPEKOL®1500 Edition 07/2008 7
Analytik Jena AG

Technical data
8 Edition 07/2008 User’s manual SPEKOL®1500
Analytik Jena AG

Unpacking instruction
Installation and transport conditions
4 Unpacking instruction
4.1 Installation and transport conditions
Installation conditions
Considering the dimensions of the SPEKOL ®1500 and a clearance of approximately
100 mm to the sides and the front, the device needs a bench space of at least 665 x 565
mm.
Caution!
Do not cover the ventilation slots of the device!
Do not place any objects on the device!
Do not operate the device in explosion-risk rooms!
Keep aggressive vapors, e.g. strongly corrosive acid or caustic vapors away from the
vicinity of the device.
The installation site of the SPEKOL®1500 must meet the following requirements:
 The place should be free of draft, dust, corrosive vapors as well as
vibrations.
 Do not set up the SPEKOL®1500 near electromagnetic fields (e.g. of
motors).
 Avoid dripping or splashing water near the instrument.
 Do not expose the device to direct sunlight or the radiation of heaters.
 Temperature range: + 15 ... + 35 °C
 Humidity: up to 85 % (at + 30 °C)
Conditions for storage and transport
For transport and storage, the following ambient conditions must be complied with:
 Temperature range in transport: -40...+70 °C
 Relative humidity in transport: up to 95 %
User’s manual SPEKOL®1500 Edition 07/2008 9
Analytik Jena AG

Unpacking instruction
Unpacking instruction
4.2 Unpacking instruction
Observe correct position of device!
Move the SPEKOL®1500 only in upright position (observe label on transport case).
Do not turn the device upside down.
Electric shock
Before connecting the SPEKOL®1500 and the PC to line power, make sure the
available line voltage agrees with the operating voltage specified on the type labels.
Operation of the devices on line voltages other than the specified operating voltage may
result in their destruction.
1. Unpack the contents carefully and check the materials.
2. Remove any obstructions or materials that could hinder the flow of air under and
around the instrument vibration.
3. Select the right voltage.
4. Plug the power cord into an outlet.
5. Make sure that the first position of the cell changer is set. Remove anything in the
sample compartment interfering with the light beam.
6. Turn on the instrument. A self test starts automatically.
Caution
Please allow the instrument to warm up for 20 minutes before taking any readings.
Your SPEKOL®1500 is now ready for operation.
10 Edition 07/2008 User’s manual SPEKOL®1500
Analytik Jena AG

Design of the SPEKOL 1500
5 Design of the SPEKOL 1500
1
2
3
1
2
3
1 Display and Button display of data’s and control buttons for use of the internal software
function
2 Sample compartment to insert accessories and sample cells
3 Cell selector arm of the Cell changer to change the position of the manual cell changer
Fig. 1 SPEKOL1500 front side
Note
Pay attention that the cover of the sample compartment must be closed during the
measurements.
4 Printer connection
5 RS 232 Interface
Fig. 2 SPEKOL1500 right side
User’s manual SPEKOL®1500 Edition 07/2008 11
Analytik Jena AG

Design of the SPEKOL 1500
6 Power cable socket
7 Printer power connection power cable socket from the printer
8 Fuse holder
9 Voltage selection to select the voltage between 220 and 110 V
10 Power switch
Fig. 3 SPEKOL1500 back side
12 Edition 07/2008 User’s manual SPEKOL®1500
Analytik Jena AG

Maintenance
Cleaning
6 Maintenance
This SPEKOL®1500 spectrophotometer has been designed to be durable and reliable.
Therefore, the routine maintenance of the instrument is minimal.
However, you will need to clean spillings or to replace the lamp or you may want check
the wavelength calibration and photometric linearity to verify the instruments
performance.
The manufacturer recommends only authorized service representatives to perform
procedures requiring removal of the instruments cover and replacement of electrical
components. To protect both of yourself and your instrument, be sure to contact the
authorized service representative to perform any service procedure, when you do not
feel comfortable performing.
6.1 Cleaning
If sample substance was spilled in the sample compartment or accessory, instantly wipe
it up using tissue paper.
For cleaning the device cover from dust or spillings, please use a soft cloth and a
commercial neutral cleanser.
Do not expose SPEKOL®1500 to corrosive atmosphere.
6.2 Replacing fuses
If the line fuses are defective, you can change them yourself.
Risk of electric shock!
Before changing the fuses, make sure to switch the SPEKOL®1500 off with the power
switch and disconnect the power cable from the power input connector of the device.
1. Remove the power cable from the SPEKOL®1500.
2. Open the bayonet connections of the fuse holders (Fig. 3 p.12) by turning to the
left using a screwdriver. The fuseholder jumps up a little.
3. Replace the defective line fuses.
Make sure to use only the following fuse types:
 T 4 AH.
4. Re-insert fuse holder and lock the bayonet connection.
5. Reconnect the power cable to the power input connector of the SPEKOL®1500
and the power outlet.
6. Switch the SPEKOL®1500 on again.
User’s manual SPEKOL®1500 Edition 07/2008 13
Analytik Jena AG

Maintenance
Replacing Halogen lamp
6.3 Replacing Halogen lamp
Electric shock!
Before changing the halogen lamp, make sure to switch the device off with the power
switch.
Caution: Risk of burns!
Allow the halogen lamp to cool down to avoid the risk of burns!
Caution: Eye injury!
Never look into the lighting lamp as the radiation may cause eye injury!
Caution!
Do not touch the glass bulb of the lamp to avoid any contamination affecting its
performance!
7. Turn the power off and unplug the instrument.
8. Put all samples and not fixed elements out of the sample compartment.
9. Place the SPEKOL 1500 carefully on a soft underlay on the top side of the device
so that you can access the underside.
Fig. 4 Lamp house cover on the under side of SPEKOL ®
10. Remove the two screws (11) and the lamp house cover (12) on the underside of the
instrument.
Fig. 5 Lamp compartment of SPEKOL®, opened
14 Edition 07/2008 User’s manual SPEKOL®1500
Analytik Jena AG

Maintenance
Replacing Deuterium lamp
11. Unscrew the holder of the halogen lamp (14) and take the holder with the halogen
lamp out of the device.
Fig. 6 SPEKOL1500 holder of the halogen lamp
12. Remove the lamp (16) from lamp holder
13. Replace the new lamp carefully in the holder.
Caution
Do not handle Halogen lamp with bare fingers. Use a piece of tissue or cloth when handling the lamp.
The oil from your fingers can cause the lamp to burn out prematurely!
14. Replace and fix the holder with the new halogen lamp in the device
15. Close the cover of the lamp house using the 2 screws.
16. Replace the device with the top side on top.
6.4 Replacing Deuterium lamp
Risk of electric shock!
Before changing the deuterium lamp, make sure to switch the device off with the power
switch.
Caution: Risk of burns!
Allow the deuterium lamp to cool down to avoid the risk of burns!
Emitted UV radiation!
Never look directly or indirectly, e.g. via a mirror, into the radiation emitted by the lamp to
avoid the risk of getting conjunctivitis!
Dirt on beam exit window!
Do not touch the glass bulb of the new deuterium lamp. Particularly avoid contamination
on the quartz glass beam exit window! If you touched the glass bulb with your fingers,
wipe the fingerprints off with a clean, lintfree cloth and pure alcohol. The performance of
the lamp will deteriorate by contamination.
1. Turn off the power and unplug the instrument
2. Put all samples and not fixed elements out of the sample compartment
User’s manual SPEKOL®1500 Edition 07/2008 15
Analytik Jena AG

Maintenance
Replacing Deuterium lamp
3. Place the SPEKOL 1500 carefully on a soft underlay on the top side of the device
so that you can access the underside
4. Remove the two screws (11) and the lamp house cover (12) on the underside of the
instrument
5. Unscrew the 2 screws of the deuterium lamp (13), disconnect the electronic plug
connection (15) and take the deuterium lamp out of the device
Do not handle Deuterium lamp with bare fingers. Use a piece of tissue or cloth when
handling the lamp. The oil from your fingers can cause the lamp to burn out prematurely!
6. Replace the new deuterium lamp and fix it with the screw
7. Close the cover of the lamp house using the 2 screws.
8. Replace the device with the top side on top.
16 Edition 07/2008 User’s manual SPEKOL®1500
Analytik Jena AG

Accessory
7 Accessory
For the installation of accessory use the two screws on the bottom of the sample
compartment.
7.1 Manual 4 cell changer
The standard configuration is a 4 cell changer for 1 cm cells. As an option there are also
4 cell changers for cells with 50 and 100 mm path length available.
Fig. 7 Manual 4 cell changer
Use the cell selector arm (Fig. 1, p.11) of the manual cell changer to set the position
of the manual cell changer.
Position 5
Position 4
Position 3
Position 2
Position 1
0%T
Sample 1
Sample 2
Sample 3
Reference
Fig. 8 Positiond of the 4 cell changer
7.2 Adjustable cell holder
Micro cells and ultra micro cells have the standard outside width of 12.5 mm, but a
reduced inside width or filling aperture of 1 to 4 mm. This requires accurate alignment of
the cell in the light beam. The adjustable cell holder can be used for cells with an
aperture height of 8,5 mm.
User’s manual SPEKOL®1500 Edition 07/2008 17
Analytik Jena AG

Accessory
Fig. 9 SPEKOL1500 adjustable cell holder
1. Insert the adjustable cell holder in the sample compartment with screw 1 to the
back site.
2. Use the screw 1 to adjust the cell horizontal to the light beam and screw 2 to adjust
the cell vertical to the light beam. The cell has the optimal position if the highest
transmittance is reached.
Together with the extension of sample compartment (8.4) and an external pump, also
flow cells can be used with this cell holder.
7.3 Round cell holder
This special cell holder can be used for round cell of standard test kits. Cells with a
diameter up to 16 mm and a height up to 110 mm can be used.
7.4 Extension of sample compartment
This extension is an attachment for tube openings that allows the use of thermostatted
cell holders. It can easily be adapted to the SPEKOL®1500 sample compartment.
Fig. 10 SPEKOL1500 extension of sample compartment
18 Edition 07/2008 User’s manual SPEKOL®1500
Analytik Jena AG