anitoa Maverick qPCR User manual

Anitoa Maverick qPCR Quick Guide
For Windows® - based Software
Version 1.66

Maverick qPCR Quick Guide
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Contents
1. Product overview.............................................................................................................................4
Features..............................................................................................................................................5
Key parameters...................................................................................................................................5
Applications.........................................................................................................................................6
Instrument external dimension............................................................................................................7
2. Hardware set-up guide....................................................................................................................8
Power up the instrument.....................................................................................................................8
Connect to a PC via USB....................................................................................................................8
Light indicator states...........................................................................................................................9
qPCR tube/plasticware requirement .................................................................................................10
Some examples of the tubes that are proven to work in Maverick instruments:...........................10
Instrument cleaning requirement ......................................................................................................12
3. Step by Step Software Guide........................................................................................................13
Install the software............................................................................................................................13
Launching the software.....................................................................................................................15
The home button ...........................................................................................................................15
Resizing the window......................................................................................................................15
The setup page –Name setup..........................................................................................................16
Samples and fluorescence channels set up..................................................................................16
Open existing experiment for template file....................................................................................17
The setup page –Cycler setup.........................................................................................................18
Melting curve analysis period........................................................................................................19
Hot lid temperature and reaction volume ......................................................................................19
Saving template.............................................................................................................................19
Auto Integration Time....................................................................................................................19
Running the experiment and monitor status .....................................................................................20
Running amplification program......................................................................................................20
Running melt analysis ...................................................................................................................21
Analysis.............................................................................................................................................22

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Amplification analysis....................................................................................................................22
Positive vs Negative results..............................................................................................................24
Standard Curve Analysis...................................................................................................................25
Steps to perform standard curve analysis:....................................................................................26
Save experiment and Print Report....................................................................................................27
4. Troubleshooting guide...................................................................................................................28
Issue with connecting the power.......................................................................................................28
The test tube would not fit, or the lid would not pressure on test tube when closed ........................29
USB connection between PC and instrument cannot be established ..............................................29
How do I know if the instrument is functioning properly when everything is plugged in?.................30
Touchscreen blank on power up.......................................................................................................31
Temperature Profile Abnormal..........................................................................................................31
Instrument is running as expected but no data were collected.........................................................31
5. PCR data processing –Positive/negative determination and Ct calculation. ...............................32
Additional data processing method –base slope correction ............................................................33
Disable data processing to view raw data for debugging purposes..................................................34
False positive detection.....................................................................................................................35
How to find the Relative strength, Amplification efficiency, and Signal to noise ratio of the reaction
data ...................................................................................................................................................38
6. Channel Crosstalk Suppression Techniques ................................................................................39
Method to adjust cross talk parameters............................................................................................41

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1. Product overview
Anitoa Maverick is a portable, high performance real time quantitative PCR system. Maverick is
equipped with a 4-channel fluorescence optical system, powered by Anitoa's ultra-low-light CMOS
bio-imaging sensor. Maverick is optimally suited for applications where portability, minimal space, fast
time-to-result is required. Applications of Maverick are point-of-care molecular diagnostics test
(POCT)
1
, food safety and environment testing, agriculture, or research lab use where bench space is
limited.
Figure 1.1 Maverick compact qPCR system
1
Clinical clearance maybe required

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Features
-Compact and rugged design. No internal moving parts, and no need for calibration.
-Multi-wavelengths 4-channels fluorescence sensing capability
2
.
-Equipped with ultra-low-light sensitive CMOS-based fluorescent imager.
-Cross platform software for Windows® and Android® OS, with cloud-ready connectivity.
-Low power. External supply with ~90W active power.
-Battery-backup option for outdoor use and power loss protection
Key parameters
Capacity (# of wells)
4, 8, 16
Channels (# of Fluorophores per well)
4 (1. FAM/SYBR Green; 2. JOE/HEX/VIC/TET;
3. ROX/Texas Red; 4. CY5/LIZ/Cy5.5)
Multiplex capability
Up to 4 targets per well
Minimum detection threshold
4 copies
Dynamic range
>1.0E9
Signal Interface
USB 2.0, Bluetooth® 2.0
Excitation source
High endurance LED
Detector
Ultra-low-light CMOS Bio-imaging chip
Thermal system
Solid-state, Peltier-based
Tube/plate formats
0.2mL, 4 or 8-tube strip
Reaction volume
10uL –60uL
Filters:
4 sets exchangeable*, **
Excitation Range:
460nm –670nm
Emission Range
510nm –720nm
DNA probes supported:
DNA binding dyes (e.g. SybrGreen), hydrolysis
probes (e.g. TaqMan probe) and hybridization
probes (e.g. FRET probes).
Temperature Uniformity
< +-0.2°C
2
Support Intercalating dyes, hydroxyls probes and FRET probes

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Temperature control resolution
+-0.1 °C
Temperature Ramp Rate
5.5°C /s heating; 4°C /s cooling
Size and weight
247mm (L) x 188mm (W) x 133mm (H).
Weight
1350g
Power supply
DC 16V, 90W (Adapter provided that accepts
110V/240V AC)
*: Wavelength characteristics can be modified by changing filter modules.
**: We support 1 –4 channels in different configurations.
Applications
•Point-of-care molecular diagnostics
3
•Food safety test
•Environmental microbial-threat monitoring
•Agriculture DNA testing
•Forensic testing
•Research and educational lab use
•Drug quality assurance testing
3
Clinical clearance required.

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Instrument external dimension
Figure 1.2 shows the external dimension of the Maverick qPCR instrument.
Figure 1.2 Maverick compact qPCR system product dimension

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2. Hardware set-up guide
Power up the instrument
The instrument is powered through an external AC/DC power adapter. The power adapter accepts
AC input ranging 110V to 240V and produces DC output of 16V
4
.
The DC power input connector port is located at the back of the instrument. Once the power is
provided, the green power indicator light will turn on.
Figure 2.1 Maverick qPCR USB and power connections
Connect to a PC via USB
The instrument is equipped with an USB port at the back, we can connect the instrument to a
Windows® based PC via the supplied USB cable.
4
10.5 V for selected custom models

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For instruments that are equipped with a touch screen, there is no need to connect the instrument to
a host PC or Tablet.
Light indicator states
There are two LED indicator lights. The green light is used to indicate power supply. As long as the
instrument is powered, the green LED indicator will stay on.

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qPCR tube/plasticware requirement
Maverick qPCR requires the use of full transparent qPCR tubes with flat caps. See figure below for
examples.
Below are drawings and dimension of the tubes we recommend both in English and Metric units
Some examples of the tubes that are proven to work in Maverick instruments:
UltraFlux® i PCR Tubes with Caps - 3247-00 from SSIBio
(https://www.ssibio.com/pcr/snapstrip-ultraflux-i-ultraflux-z-pcr-tubes/ultraflux-i-pcr-tubes-with-
caps/3247-00-detail)
EasyStrip™ Plus Tube Strip with Attached Ultra Clear Caps AB2005 from Thermo Fisher