anitoa Maverick qPCR User manual

The instruction manual must be properly placed in the product box during shipment.
The user is required to keep this manual in a safe place so that it can be consulted when
needed.
All rights reserved. All information contained in this manual is subject to change without
notice.
Ultra-Portable Real-Time Fluorescence
Quantitative PCR Instrument
Maverick qPCR
Instruction Manual
Version（V2.1）
Manufacturer: Anitoa Biotechnology (Hangzhou) Co., Ltd.
Address: China-Australia Biomedical Industry Science and Technology Park, No.656
Binan Road, Binjiang District, Hangzhou, Zhejiang, China
Thank you for choosing our products. Please read this instruction manual carefully before use.

Anitoa warrants that the MQ4 Fluorescence PCR instrument you are using has been
fully tested and meets the requirements of the instructions for use. The
instructions and safety warnings given in this instruction manual must be followed
in order to use the instrument, otherwise the warranty does not apply.
Software Description：
The software is a necessary tool for the operation of the instrument and Anitoa has
the right to modify its functions or design, etc., in order to improve its operational
performance and reliability, without prior or subsequent notification to the customer,
and Anitoa has all the intellectual property rights for the modified version.
Responsibility Statement：
Anitoa is not responsible for direct or indirect incidental damages arising from
non-compliance with the operating instructions or incorrect use of the MQ4 Real Time
Fluorescence PCR instrument. Anitoa's responsibility is limited to the repair of the
machine and the replacement of parts, and is not responsible for the results of the
experiments.
Intellectual Property Statement:
The copyright for this manual and other proprietary information provided is owned
by Anitoa. The information in this instruction manual may only be used for
installation, training and service purposes as originally intended. Anitoa holds
the copyright to the software referred to in this manual and grants the customer
the right to use the software.

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CONTENTS
Chapter 1 Important Notes ......................................................... 1
1.1 Instrument Grounding .......................................................... 1
1.2 Placement of the Instrument ................................................... 1
1.3 Operation Note ................................................................ 2
1.4 After-Sales Service ........................................................... 2
1.5 Packaging, Storage and Transportation Marking ................................. 3
1.6 Instrument Identification Information ......................................... 4
1.7 FCC Statement ................................................................. 5
Chapter 2 Product Overview ........................................................ 6
2.1 Product Usage ................................................................. 6
2.2 Product Features .............................................................. 6
2.3 Specification Model Description .............................................. 7
2.4 Instrument Models ............................................................. 7
2.5 Main Technical Parameters ..................................................... 7
2.6 WIFI and BT ................................................................... 8
Chapter 3 Instrument Installation ................................................ 11
3.1 Environmental Conditions ..................................................... 11
3.2 Unpacking .................................................................... 11
3.3 Comparison of packing list ................................................... 11
3.4 Power Cord Connection ........................................................ 12
3.5 Instrument Usage ............................................................. 12
Chapter 4 Software Operation Guide ............................................... 14
4.1 Launch Software .............................................................. 14
4.2 Experimental Setup ........................................................... 15
4.3 Save Template ................................................................ 17
4.4 Running Experiments .......................................................... 18
4.5 Experimental analysis ........................................................ 18
4.6 Data Export .................................................................. 21
Chapter 5 Instrument Maintenance ................................................. 25
5.1 Instrument Cleaning .......................................................... 25
5.2 Instrument Protection ........................................................ 25
5.3 Waste Disposal ............................................................... 25
5.4 Overheat Protection .......................................................... 25
5.5 Operation Requirements ....................................................... 26
Chapter 6 FAQ .................................................................... 27

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Chapter 1 Important Notes
The following basic safety measures must be observed during all phases of
operation, maintenance and repair of this instrument. Failure to observe these
measures or the warnings and precautions indicated in this manual will likely affect
the basic protection provided by the instrument. It will also undermine the safety
standards for which the instrument was designed and manufactured, as well as the
intended scope of use of the instrument.
1.1 Instrument Grounding
To ensure the personal safety of the operator, please use the manufacturer's power
adapter with a 10A three-prong grounded plug at the input. Use a ground socket matching
the plug to ensure that the input power cable of the instrument is reliably grounded.
1) Use of power supply
Before the instrument adapter is connected to the power cord, you must ensure
that the voltage (100 to 240 VAC, 50/60Hz, 5A) and frequency of the AC power supply
are consistent with those required by the instrument adapter. When making the power
cord connection, you must ensure that the instrument power switch is off. Do not touch
the power switch and power cord with wet hands. It is prohibited to disconnect the
power cord when the instrument is not powered off. It is forbidden to touch the power
cord to the hot surface of the instrument. Do not clean the instrument when it is
not disconnected. Please turn off the power when the instrument is no longer in use.
2) Power cord
The instrument should normally use the power cord supplied with it. If the power
cord is broken, it must be replaced without repair. When replacing the power cord,
it must be replaced with a power cord of the same type and size. When using this
instrument, do not press anything on the power cord and do not place the power cord
in a place where people move around.
3) Power cord plugging and unplugging
Power cord plugging and unplugging must be the correct handheld plug operating
parts, plug insertion should ensure that the plug is completely, tightly inserted
into the socket, pull out the plug do not hard pull, yank the power cord.
1.2 Placement of the Instrument
1) The instrument should not be placed in a location where it is difficult to cut off the power.

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2) The instrument uses semiconductor cooling and fan-assisted heat dissipation, so when placing
the instrument, ensure that there are no obstacles within 15 cm of the instrument.
3) The instrument should be placed in a place with low humidity, less dust and far from water
sources (such as pools, water pipes, etc.), with good ventilation, no corrosive gases or strong
magnetic fields, and avoid direct sunlight and strong light sources. Do not place the instrument
in a humid or dusty place. The bench where the instrument is placed should be horizontal and
stable.
4) High ambient temperature may affect the performance of the instrument or cause malfunction.
Do not use the instrument in direct sunlight and strong light source, so as not to affect the
fluorescence detection of the instrument, and should be far away from the heating, stove and all
other heat sources.
5) Turn off the power when you stop working, and when you don't use the instrument for a long
time, cut off the power, unplug it, and cover it with soft cloth or plastic film to prevent dust and
foreign matter from entering.
1.3 Operation Note
1) During the test operation, avoid liquid dripping on the instrument.
2) The consumables and reagents used in the test should be disposed of according to the relevant
standards, and should not be discarded or dumped at will.
3) If there are hazardous substances in the test, they should be operated only after relevant
training before use.
4) After use, the hazardous substances should be properly handled and stored in strict accordance
with the instructions for their use.
5) The test personnel who operate the instrument need to be trained and have relevant
qualifications
6) When handling toxic, corrosive or infectious substances, safety goggles and gloves must be
worn.
7) When the instrument is running and just after a period of operation, it is strictly prohibited to
touch the metal module to avoid burns.
8) It is strictly forbidden to open the instrument during the operation of the machine, otherwise it
will cause permanent damage to the instrument.
1.4 After-Sales Service
1) After receiving the instrument, please confirm the relevant content on the after-sales warranty
card and contact the shipping unit if you have any questions.
2) After unpacking the instrument, please keep the packing box and packing materials properly
for use in maintenance.
3) Before the instrument is sent to the maintenance department, the instrument must be
disinfected.
4) After the instrument is delivered to the maintenance department and unpacked, the
maintenance personnel must disinfect the instrument immediately.

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1.5 Packaging, Storage and Transportation Marking
Table 1.5 Package storage and transportation identification
Symbol
Title
Description
Position
Place
carefully
and gently
This symbol is used to indicate that
the product is a precision
instrument and should be handled
carefully and gently.
On the packing
carton
Upward
This symbol is used to indicate that
the instrument must be kept upward
during handling, storage and use, and
must not be placed sideways or upside
down to avoid damage to the
instrument.
On the packing
carton
Afraid of
getting wet
This symbol is used to indicate that
the instrument must not be stored in
a humid environment or in a place
where it will be splashed with
liquid.
On the packing
carton
Stacking 5
layers
This symbol is used to indicate the
maximum number of layers of vertical
stacking overlap allowed for a box.
On the packing
carton
Anti-bumpin
g
This symbol is used to indicate that
the instrument should be handled,
stored and used with care to avoid
any impact on the performance of the
instrument.
On the packing
carton

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1.6 Instrument Identification Information
Table 1.6 Instrument identification information
Symbol
Description
The location on the instrument
where the symbol will appear
Watch out for high
temperatures
On the equipment
Production date
On the equipment nameplate
CE mark
On the equipment nameplate
Pay attention to safety
On the equipment nameplate
In vitro diagnostic
medical devices
On the equipment nameplate
E-waste classification
On the equipment nameplate
Product number
On the equipment nameplate
Serial number
On the equipment nameplate
Instruction manual
On the equipment nameplate
Biological hazards
On the equipment nameplate

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1.7 FCC Statement
This device complies with part 15 of the FCC rules. Operation is subject to the following two
conditions: (1) this device may not cause harmful interference, and (2) this device must accept
any interference received, including interference that may cause undesired
operation.
Changes or modifications not expressly approved by the party responsible for compliance could
void the user’s authority to operate the equipment.
NOTE: This equipment has been tested and found to comply with the limits for a Class B digital
device, pursuant to part 15 of the FCC Rules. These limits are designed to provide reasonable
protection against harmful interference in a residential installation. This equipment generates
uses and can radiate radio frequency energy and, if not installed and used in accordance with
the instructions, may cause harmful interference to radio communications. However, there is no
guarantee that interference will not occur in a particular installation. If this equipment does
cause harmful interference to radio or television reception, which can be determined by turning
the equipment off and on, the user is encouraged to try to correct the interference by one or
more of the following measures:
•Reorient or relocate the receiving antenna.
•Increase the separation between the equipment and receiver.
•Connect the equipment into an outlet on a circuit different from that to which the receiver is
connected.
•Consult the dealer or an experienced radio/TV technician for help.
Important Note:
Radiation Exposure Statement
This equipment complies with FCC radiation exposure limits set forth for an uncontrolled
environment. This equipment should be installed and operated with minimum distance 20cm
between the radiator and your body.
This transmitter must not be co-located or operating in conjunction with any other antenna or
transmitter.

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Chapter 2 Product Overview
This chapter mainly describes the usage, characteristics, specifications and performance
parameters of MQ4 fluorescence quantitative PCR instrument.
2.1 Product Usage
The MQ4 model is an ultra-portable qPCR instrument with a large 10" touch screen for integrated
operation. The product is developed based on our multi-channel fluorescence imaging optical
system with CMOS bio-image sensor. The product can be widely used in universities and research
institutes, CDC, Entry-Exit Inspection and Quarantine Bureau, Public Security Criminal Evidence
Identification Center, veterinary stations, food companies and pharmaceutical companies.
2.2 Product Features
1) Efficient and fast: the use of ultra-fast temperature rise and fall system and unique chip
technology, no specific supplies to achieve 30 min fast test.
2) Touch operation: large 10-inch LCD touch screen, integrated touch operation, no external
computer, simple and convenient.
3) Lightweight and portable: the machine is small and portable (247 * 188 * 133 mm), weighing
only 2.6 kg, easy to move, especially for the laboratory testing space limited places.
4) Stable and reliable: The whole machine has no moving parts, and the structure is sturdy
and durable, so there is no need for regular calibration even after long time use.
5) Intelligent management: optional 4G module allows remote management or cloud management
of experimental data as needed.
6) Multiple options: Support 2/4 fluorescence channels (more channels can be customized),
suitable for most of current dyes, no cross interference between channels, no need for
regular calibration maintenance.
7) Stable light source: independent LED light source for each fluorescence channel, stable
and non-decaying LED light source, no need for regular replacement.
8) High sensitivity chip: unique "Ultra-Low-Light CMOS Image Sensor (CIS) Chips", millisecond
speed shooting without row-by-row scanning, stable and reliable data. Highly integrated
IC chip technology makes the instrument structure simple, stable and reliable, all digital
circuitry anti-interference.

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2.3 Specification Model Description
2.4 Instrument Models
Table 2.4 Instrument models
Model
Channel
Sample throughput
MQ4044
4 channels
4 wells
MQ4162
2 channels
16 wells
MQ4164
4 channels
16 wells
2.5 Main Technical Parameters
Table 2.5 Main technical parameters
Basic Performance
Dimension
247*188*133mm
Bare Weight
2.6 kg
Power
DC 15V 9.6A
Noise Level
≤50dB
Communication Interface
TypeA
PCR System Performance
Sample Volume
10～50ul
Applicable Consumables
Transparent 0.2ml single tube, 8*0.2ml row tube
Temperature Accuracy
≤0.5℃
Detection Repeatability
Ct CV≤2%
4：Represents the number of channels of
the instrument
16：Represents sample throughputs
4： Represents appearance number
MQ：Represents series number
MQ 4 16 4

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Fluorescence Detection System Performance
Light Source
High Brightness LED
Detector
Ultra-Low-Light CMOS Image Sensor (CIS) Chips
Excitation Wavelength
F1：470nm F2：523nm
F3：571nm F4：624nm
Detection Wavelength
F1：527nm F2：564nm
F3：612nm F4：694nm
2.6 WIFI and BT
The XY8788 module provides a shared antenna interface ANT_WIFI/BT for WIFI and BT with an
impedance of 50Ω . Customers can connect external PCB antenna, suction cup antenna or ceramic
antenna through this interface to realize WIFI and BT functions.
2.6.1 WIFI overview
XY8788 module supports 2.4G/5G dual-band WLAN wireless communication, supports 802.11a,
802.11b, 802.11g, 802.11n and 802.11ac and other standards, the highest rate can reach 150Mbps.
Its characteristics are as follows:
Support Wake-on-WLAN (WoWLAN)
Support ad hoc mode
Support WAPI SMS4 hardware encryption
Support AP (HotSpot 2.0 ) mode
Support Wi-Fi Direct
Support HT20 MCS7 andVHT80 MCS9

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2.6.2 WIFI performance indicators
The following table lists the transmit and receive performance of XY8788 WIFI
Table 24: Transmission performance of WIFI
standard rate
802.11b 1Mbps
802.11
Output Power
17±2dBm
b 11Mbps
802.11g 6Mbps
2.4G
H
16±2dBm
z802.11g 54Mbps
802.11n HT2
15±2dBm
15±2dBm
0 MCS0
802.11n HT2
14±2dBm
0 MCS7
802.11n HT4
13±2dBm
0 MCS0
802.11n HT4
10±2dBm
0 MCS7
802.11
10±2dBm
a 6Mbps
802.11
10±2dBm
a 54Mbps
802.11n HT20 MCS0
5GH
10±2dBm
z802.11n HT20 MCS7
802.11n HT4
10±2dBm
10±2dBm
0 MCS0
802.11n HT4
10±2dBm
0 MCS7 10±2dBm
Table 25: Receive performance of WIFI
standard rate Sensitivity
802.11b 1Mbps -87dBm
802.11b 11Mbps -87dBm
802.11g 6Mbps -91dBm
2.4G
Hz
802.11g 54Mbps -76dBm
802.11n HT20 MCS0 -90dBm
802.11n HT20 MCS7 -73dBm
802.11n HT40 MCS0 -87dBm
802.11n HT40 MCS7 -68dBm
802.11a 6Mbps -90dbm
802.11a 54Mbps -74dbm
802.11n HT20 MCS0 -88dbm
5GH
z802.11n HT20 MCS7 -69dbm
802.11n HT40 MCS0 -86dbm
802.11n HT40 MCS7 -66dbm

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Reference Specification
serial
numb
er
document number
1
IEEE 802.11n WLAN MAC and PHY, October 2009 + IEEE 802.11-2007 WLAN MAC and PHY
PHY, June 2007
2
IEEE Std 802.11b, IEEE Std 802.11d, IEEE Std 802.11e, IEEE Std
802.11g, IEEE Std 802.11i: IEEE 802.11-2007 WLAN MAC and PHY, June 2007
2.6.3 BT overview
XY8788 module supports BT v2.1+EDR, 3.0+HS, v4.1+HS, V4.2 . The modulation mode
supports GFSK, 8PSK, π/4QPSK.
Supports up to 7 wireless connections.
Up to 3.5 PICONET piconets are supported simultaneously.
Support 1 -way SCO oreSCO connection(Synchronous Connection Oriented).
The first channel starts at 2402 MHz with one channel every 1 MHz to 2480 MHz.
BT 4.0 channel width is 2 MHz spacing,
Holds 40 channels
Table 26: BT rate and version information
Version
data rate
Maximum
application
throughput
Remark
1.2
1Mbit/s
>80 Kbit/s
2.0+EDR
3Mbit/s
>80 Kbit/s
3.0+HS
24 Mbit/s
Please refer to
3.0+HS
4.0
24 Mbit/s
Please refer to 4.0
LE
Reference Specification
serial
numb
er
document number
1
Bluetooth Radio Frequency TSS and TP Specification 1.2/2.0/2.0 +
EDR/2.1/2.1+EDR/3.0/3.0 + HS, August 6, 2009
2
Bluetooth Low 能源 RFPHY Test Specification,RF-PHY.TS/4.0.0, December 15, 2009

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Chapter 3 Instrument Installation
This chapter describes the use and storage conditions of the MQ4 ultra-portable
quantitative fluorescence PCR instrument, its construction, removal of the fixture,
installation/uninstallation of the software, and preparation for power-up.
3.1 Environmental Conditions
1) Transportation and storage conditions of the instruments
a. Environmental temperature：－20℃～55℃ ；
b. Relative humidity：≤85％
2) Working conditions requirements
a. Environmental temperature：15℃~35℃
b. Environmental humidity：≤85％
c. Input voltage： DC 15V 9.6A
3.2 Unpacking
1) The outer packaging of the product is a cardboard box, filled with shock-absorbing
foam inside, after unpacking, first check whether the items you receive are missing
and damaged.
2) If the outer packaging of the product is obviously damaged during transportation,
please do not use it and contact the seller in time.
3) Check the supplied accessories against the packing list (in the instruction manual)
to see if they are complete.
4) If the instrument or accessories have been damaged or lost in transit, please inform
the shipping company personnel and our customer service personnel.
3.3 Comparison of packing list
After opening the box, please check and accept the items in the box according
to the packing list items, if you find that the items are damaged or missing,
please contact the supplier immediately.
Table 3.4 Packing list
Annexes
Quantity
Fluorescence
Quantitative PCR
Instrument
1
Power Cord
1

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Flip Cover Control Switch
Power Jack
Boat Switch
Power Adapter
1
USB Cable
1
Instruction Manual
1
Factory Inspection
Report
1
Warranty Card
1
Certificate of
Conformity
1
3.4 Power Cord Connection
1) Connection of adapter: the adapter supplied with the instrument should be used
to connect the adapter to the instrument.
2) Connection of power cord: the power cord supplied with the instrument should be
used. When connecting, the instrument power switch should be in the off state,
and then turn on the instrument switch after connecting.
3.5 Instrument Usage
Figure 3.6-1 Back of the instrument

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SampleTable
Location
Instrument Running
Indicator Light
Figure 3.6-2 Front of the instrument
1) Insert the DC connector into the instrument interface, and the "click" sound means that the
connector is in place
2) Open the main switch of the instrument (boat switch), and the red indicator of the instrument
will light up
3) Open the cover of the instrument, and place the test tube with collected samples on the sample
table in the picture
4) Close the flip cover and open the screen version software for use. The green indicator will light
up after the instrument is started normally.

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Chapter 4 Software Operation Guide
Anitoa qPCR software can be used to set up experiments, run experiments, collect
data, analyze and manage experiments. The software contains four main functional
modules, namely "User", "Test", "Results", and "System".
1) "User" module: mainly contains upload configuration, user management, image, and
manufacturer settings.
2) "Test" module: mainly contains the new experiments, experimental parameters settings,
running experiments, viewing and analyzing experimental results.
3) "Results" module: mainly contains experimental data analysis, upload, export, standard curve.
4) "System" module: mainly contains WLAN, language, virtual keyboard, date and time, display,
and application language.
4.1 Launch Software
1) Launch the screen software "anitoa" to enter the login screen, as shown in Figure 4.1-1, and click
the "Login" button.
Figure 4.1-1 Login Interface

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2) When asked if the USB connection is allowed, click < OK>, as shown in Figure 4.1-2
Figure 4.1-2 USB Access interface
4.2 Experimental Setup
1) In the test interface, click "New Experiment" (Figure 4.2-1) to enter the experiment
settings 1
Figure 4.2-1 New experiment interface

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2) In Experiment Setup 1 (Figure 4.2-2), enter the experiment name, select the
corresponding channel, select the well position, and enter the sample information,
etc., and click "Next" to enter Experiment Setup 2.
Figure 4.2-2 Experimental setup 1
3) In Experimental Setup 2 (Figure 4.2-3), set the reaction program (parameters such
as reaction temperature, reaction time, number of cycles, photo stage,
etc.)
Figure 4.2-3 Experimental setup2

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4.3 Save Template
1) Click the "Save Template" icon in the upper right corner to save the current
template.
Figure 4.3-1 Program setting interface
2) The saved templates will appear in the "Test" screen for selection. Click "Import
Template" in the upper right corner to import other experiment templates.
Figure 4.3-2 Template Page