anitoa MQ6 Series User manual

Multi-Bay Real-Time Fluorescence
Quantitative PCR System
Maverick qPCR
（MQ6 / MY6 / MQ8 / MY8 Series）
Instruction Manual
Version（V1.0）
Manufacturer: Anitoa Biotechnology (Hangzhou) Co., Ltd.
Address: China-Australia Biomedical Industry Science and Technology Park,
No.656 Binan Road, Binjiang District, Hangzhou, Zhejiang, China
Thank you for choosing our products. Please read this instruction manual carefully before
use.
The instruction manual must be properly placed in the product box during shipment.
The user is required to keep this manual in a safe place so that it can be consulted
when needed.
All rights reserved. All information contained in this manual is subject to change
without notice.

Anitoa Biotechnology (Hangzhou) Co., Ltd.(hereinafter referredto as Anitoa) guarantees
that the Multi-Bay Real-Time Fluorescent Quantitative PCR System you are using has
been fully tested and meets the requirements of the instructions for use. The instructions
and safety warnings given in this instruction manual must be followed in order to use the
instrument, otherwise the warranty does not apply.
Software description
The software is a necessary tool for the operation of the instrument. For the purpose of
improving its performance and reliability, Anitoa has the right to modify its functions or
design, etc., in advance or subsequently without informing the clients, and Anitoa has all the
intellectual property rights of the modified version.
Responsibility statement
Anitoa is not responsible for direct or indirect incidental damages arising from non-
compliance with the operating procedures given in the instructions or incorrect use of the
Multi-Bay Real-Time Fluorescent Quantitative PCR System. Only Anitoa 's technicians or its
authorized agents may inspect or provide parts for the instrument in question, and we are
not responsible for direct or indirect collateral damage resulting from the user's disassembly
and replacement of parts. The responsibility of Anitoa is limited to the repair of the machine
and the replacement of the parts, but not for the results of the experiments.
Intellectual property statement
Anitoa owns the copyright for this manual and other proprietary information provided.
The information in this instruction manual may only be used for installation, training, and
service as intended. Any copying, reproduction, or translation of this information, in whole
or in part, into other languages, or other processes not mentioned herein, whether by
electronic or written means, is prohibited without the prior written consent of Anitoa.
Anitoa holds the copyright to the software mentioned in this instruction manual and has
the right to grant the customer the right to use the software.

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CONTENTS
Chapter 1 Important Notes........................................................ 1
1.1 Instrument grounding ...................................................... 1
1.2 Placement of the Instrument ................................................ 1
1.3 Precautions .............................................................. 2
1.4 After-sales service......................................................... 2
1.5 Packaging, storage and transportation marking................................ 3
1.6 Instrument Identification Information ......................................... 4
Chapter 2 Product Overview ...................................................... 5
2.1 Product usage ............................................................ 5
2.2 Product Features.......................................................... 5
2.3 Specification model description ............................................. 6
2.4 Instrument models......................................................... 6
2.5 Main technical parameters.................................................. 7
Chapter 3 Instrument Installation.................................................. 9
3.1 Environmental conditions................................................... 9
3.2 Unpacking ............................................................... 9
3.3 Check the packing list...................................................... 9
3.4 Power cord connection.................................................... 10
3.5 How to use the instrument................................................. 10
Chapter 4 Software Operation Guide ............................................. 12
4.1 System introduction ...................................................... 13
4.2 Home page.............................................................. 16
4.3 Amplification programs.................................................... 23
4.4 Test results.............................................................. 25
Chapter 5 Instrument Maintenance ............................................... 30
5.1 Instrument cleaning....................................................... 30
5.2 Instrument protection ..................................................... 30
5.3 Waste disposal .......................................................... 30
5.4 Overheat protection ...................................................... 30
5.5 Operation requirements ................................................... 31
Chapter 6 FAQ ................................................................. 32

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Chapter 1 Important Notes
The following safety measures must be observed during all phases of operation,
maintenance, and servicing of this instrument. Failure to observe these measures or the
warnings and precautions indicated in this manual will likely affect the basic protection
provided by the instrument. Also, this can undermine the safety standards for which the
instrument was designedandmanufacturedandtheintendedscopeofuseoftheinstrument.
1.1 Instrument grounding
To ensure the personal safety of the operator, please use three-prong grounding plug
provided by the manufacturer. When using the adapter, please use a grounding socket that
matches the plug to ensure that the input power line of the instrument is reliably grounded.
1) Use of power supply
Before the instrument adapter is connected to the power cord, it must be ensured that
the ACpower supplyvoltage (100to 240VAC) andfrequency (50/60Hz) are consistent with
those required by the instrument adapter. When making the power cord connection, make
sure that the instrument power switch is off. Do not touch the power switch and power cord
with wet hands. It is prohibited to disconnect the power cord when the instrument is not
powered off. It is forbidden to touch the power cord to the hot surface of the instrument. Do
not clean the instrument when it is not disconnected. Please turn off the power when the
instrument is no longer in use.
2) Power cord
The instrument should normally use the power cord supplied with it. If the power cord
is broken, it must be replaced without repair. When replacing the power cord, it must be
replaced with the same type of power cord of the same specifications. When this instrument
is in use, do not place anything on the power cord, and do not place the power cord in a
place where people move around.
3) Power cord plugging and unplugging
Power cord plugging and unplugging must be the correct handheld plug operating parts,
plug insertion should ensure that the plug is completely, tightly inserted into the socket, do
not pull hard when pulling out the plug, or yank the power cord.
1.2 Placement of the Instrument
1) The instrument should not be placed in a location where it is difficult to disconnect the
power supply.
2) The instrument uses semiconductor cooling and fan-assisted heat dissipation, so when
placing the instrument, ensure that there is no obstacle within 15cm around the
instrument, and when multiple instruments are used at the same time, the distance
between each instrument should be not less than 30 cm.

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3) The instrument should be placed in a place with low humidity, less dust, and far from
water sources (such as pools, water pipes, etc.), with good ventilation, no corrosive gas
or strong magnetic field interference, and avoiding direct sunlight and strong light
sources. The table where the instrument is placed should be horizontal and stable.
4) High ambient temperature will affect the testing performance of the instrument or cause
malfunction. Do not use this instrument in the place of direct sunlight and strong light
source to avoid affecting the fluorescence detection of the instrument, and should be far
away from heating, stove and all other heat sources.
5) Turn off the power when stop working. When the instrument is not used for a long time,
cut off the power, unplug it, and cover it with soft cloth or plastic film to prevent dust and
foreign objects from entering.
1.3 Precautions
1) During the test operation, avoid liquid dripping on the instrument.
2) The consumables and reagents used in the test should be disposed of according to the
relevant standards, and should not be discarded or dumped at will.
3) If there are hazardous substances in the test, related training must be conducted before
using them.
4) After use, the hazardous substances should be handled and stored properly in strict
accordance with the relevant regulations.
5) The test personnel who operate the instrument need to be trained and have relevant
qualifications.
6) When handling toxic, corrosive or infectious substances, safety goggles and gloves
must be worn.
7) It is strictly forbidden to touch the metal module when the instrument is running and for
a period of time just after the operation to avoid burns.
8) It is strictly forbidden to open the instrument during the operation of the instrument,
otherwise it will cause abnormal experimental results.
1.4 After-sales service
1) After receiving the instrument, please confirm the relevant content on the after-sales
warranty card and contact the shipping unit if you have any questions.
2) After unpacking the instrument, please keep the packing box and packing materials
properly for use when returning to the factory for after-sales service.
3) Before sending the instrument to the maintenance department, the instrument must be
disinfected.
4) After the instrument is delivered to the maintenance department and unpacked, the
maintenance personnel must disinfect the instrument immediately.

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1.5 Packaging, storage and transportation marking
Table 1-5-1 Package storage and transportation identification
Symbol
Title
Description
Position
Place
carefully
and gently
This symbol is used to indicate that
the product is a precision instrument
and should be handled carefully and
gently.
On the packing
carton
Upward
This symbol is used to indicate that the
instrument must be kept upward during
handling, storage and use, and must
not be placed sideways or upside
down to avoid damage to the
instrument.
On the packing
carton
Afraid of
getting wet
This symbol is used to indicate that
the instrument must not be stored in a
humid environment or in a place
where it can be splashed with liquid.
On the packing
carton
Anti-
bumping
This symbol is used to indicate that
the instrument should be handled,
stored, and used with care to avoid
any impact on the performance of the
instrument.
On the packing
carton

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1.6 Instrument Identification Information
Table 1-6-1 Instrument identification information
Symbol
Description
The location on the instrument
where the symbol will appear
Watch out for high
temperatures
On the equipment
Production date
On the equipment nameplate
Pay attention to safety
On the equipment nameplate
Serial number
On the equipment nameplate
Instruction manual
On the equipment nameplate
Biological hazards
On the equipment nameplate

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Chapter 2 Product Overview
This chapter mainly describes the usage, characteristics, specifications and
performance parameters of multi-bay real-time fluorescence quantitative PCR system.
2.1 Product usage
Multi-bay real-time fluorescence qPCR system is a series of qPCR instruments
consisting of multiple single chambers (16 wells) that can be freely combined according to
demand. Each chamber can be operated independently without interference, so that
samples can be tested as they arrive, greatly improving the flexibility of the test, and up to
96 wells can be tested simultaneously to achieve high throughput testing needs. The
instrument comes with a 10-inch touch screen for integrated operations such as testing and
data analysis.
The product is developed based on our multi-channel fluorescence imaging optical
system with CMOS bio-image sensor. The product can be widely used in universities and
research institutes, CDC, Entry-Exit Inspection and Quarantine Bureau, Public Security
Criminal Evidence Identification Center, veterinary stations, food companies and
pharmaceutical companies.
2.2 Product Features
1) Efficient and fast: with ultra-fast temperature rise and fall system and unique chip
technology, fast detection can be achieved.
2) Touch operation: 10-inch LCD touch screen, integrated touch operation, no external
computer, simple and convenient.
3) Testing flexibility: the machine can have up to 6 independent chambers, which can
be operated jointly or independently to achieve the "on-the-go" inspection of
samples.
4) Stable and reliable: the whole machine has no moving parts, and the structure is
sturdy and durable without regular calibration even after a long time of use.
5) Intelligent management: optional 4G module allows remote management or cloud
management of experimental data as needed.
6) Multiple options: Support 2 or 4 fluorescence channels (more channels can be
customized), suitable for most of current dyes, no cross interference between
channels, no need for regular calibration maintenance.
7) Stable light source: independent LED light source for each fluorescence channel,
stable and non-decaying LED light source, no need for regular replacement.
8) High sensitivity chip: the unique "Ultra-Low-Light CMOS Image Sensor (CIS) Chips",

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millisecond-level extremely fast shooting without row-by-row scanning, stable and
reliable data.
9) Modularity: realize the modularity of single chamber, which can be integrated with
other systems and has wider application scenarios. The physical picture of the
single chamber is shown in Figure 2-2-1.
Figure 2-2-1 Single chamber module
2.3 Specification model description
Example:
2.4 Instrument models Table 2-4-1 Instrument models
Model
Channel
Sample throughput
Applicable consumable
MQ6962
2 channels
96 wells
0.2ml standard PCR tubes
MQ6964
4 channels
96 wells
0.2ml standard PCR tubes
MY6962
2 channels
96 wells
Y-type PCR tubes
MY6964
4 channels
96 wells
Y-type PCR tubes
Number of instrument channels
Sample throughputs
Appearance number
Series number
XX X XX X

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MQ8482
2 channels
48 wells
0.2ml standard PCR tubes
MQ8484
4 channels
48 wells
0.2ml standard PCR tubes
MY8482
2 channels
48 wells
Y-type PCR tubes
MY8484
4 channels
48 wells
Y-type PCR tubes
2.5 Main technical parameters
Table 2-5-1 MQ6 / MY6 main technical parameters
Basic Performance
Dimension
483*541*252 mm
Bare weight
22 kg
Power
100~240VAC，50/60Hz
Noise level
≤ 60dB
PCR System Performance
Sample volume
10～60ul
Sample throughput
96-well (6×16-well, 6-bay module)
Applicable consumables
Transparent 0.2ml single tube, 8*0.2ml row
tube, Y-type tube
Experimental operation
mode
Independent operation or free combination of
six 16-well experiments
Maximum heating rate
≥ 8.0℃/s
Maximum cooling rate
≥ 5.5℃/s
Temperature accuracy
≤ 0.5℃
Detection repeatability
Ct CV≤ 2%
Fluorescence Detection System Performance
Light source
High brightness LED
Detector
Ultra-low-light CMOS Bio-imaging chip
Excitation wavelength
F1：470nm F2：523nm
F3：571nm F4：624nm
Detection wavelength
F1：527nm F2：564nm
F3：612nm F4：694nm

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Table 2-5-2 MQ8 / MY8 main technical parameters
Basic Performance
Dimension
483*286*252 mm
Bare weight
11 kg
Power
100~240VAC，50/60Hz
Noise level
≤ 60dB
PCR System Performance
Sample volume
10～60ul
Sample throughput
48-well (3×16-well, 3-bay module)
Applicable consumables
Transparent 0.2ml single tube, 8*0.2ml row
tube, Y-type tube
Experimental operation
mode
Independent operation or free combination of
three 16-well experiments
Maximum heating rate
≥ 8.0℃/s
Maximum cooling rate
≥ 5.5℃/s
Temperature accuracy
≤ 0.5℃
Detection repeatability
Ct CV≤ 2%
Fluorescence Detection System Performance
Light source
High brightness LED
Detector
Ultra-low-light CMOS Bio-imaging chip
Excitation wavelength
F1：470nm F2：523nm
F3：571nm F4：624nm
Detection wavelength
F1：527nm F2：564nm
F3：612nm F4：694nm

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Chapter 3 Instrument Installation
Thischapterdescribes theuseandstorage conditions ofthemulti-bayreal-timefluorescence
quantitativePCRinstrument,itsstructuralcomponents,removalofthefixture,installation/
uninstallationofthesoftware,andpreparationforpower-up.ThischaptertakesMQ6964asan
example.
3.1 Environmental conditions
1) Transportation and storage conditions of the instrument
a. Environmental temperature: －5℃～40℃；
b. Relative humidity: ≤75％
2) Working conditions requirements
a. Environmental temperature: 15℃~35℃
b. Environmental humidity: 35%~75％
c. Input voltage: 100～240VAC, 50/60Hz
3.2 Unpacking
1) The outer packaging of the product is a cardboard box, filled with shock-absorbing foam
inside, after unpacking, first check whether the items you receive are missing or
damaged.
2) If the outer packaging of the product is obviously damaged during transportation, please
do not use it and contact the manufacturer and authorized distributor in time.
3) Check the completeness of the provided accessories against the packing list (Table 3-3-
1).
4) If the instrument or accessories have been damaged or lost in transit, please inform the
shipping company personnel and our customer service personnel.
3.3 Check the packing list
After opening the box, please check the contents of the box according to the
packing list, if the items are found to be damaged or missing, please contact the
manufacturer and the authorized distributor immediately.
Table 3-3-1 Packing list
Accessory
Quantity
Multi-Bay Fluorescence
Quantitative PCR System
1

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Power Cord
1
Instruction Manual
1
Factory Inspection Report
1
Warranty Card
1
Certificate of Conformity
1
USB Flash Drive
1
3.4 Power cord connection
Use the power cord supplied with the instrument. When connecting, the instrument
power switch should be in the "off" state, and then turn on the instrument switch after
connecting.
3.5 How to use the instrument
Figure 3-5-1 Front of the instrument
Sample Well Lid
Sample Well
Location
Touch Screen
Power Indicator
Light

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Figure 3-5-2 Back of the instrument
1) Insert the DC connector into the power jack of the instrument.
2) Turn on the instrument power switch, the power switch lights up red and the
instrument power indicator (orange) lights up.
3) Click the "Open the Chambers" button on the touch screen to open the lids of the
sample well locations and put the collected tubes into the sample wells.
4) Close the sample well lids, operate the touch screen panel and start the experiment.
Power Switch
Power Jack

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Chapter 4 Software Operation Guide
This chapter introduces the use of the multi-bay real-time fluorescence
quantitative PCR instrument software and the operation related to experimental data.
The contents of this chapter take MQ6964 as an example.
Anitoa qPCR software can be used to set up experiments, run experiments, and
collect, analyze and manage experimental data. The software contains five main
functional modules, namely "Home Page" "Amplification Programs" "Test Results"
"System Settings" "User".
1) "System Settings" module: mainly contains WLAN, language, virtual keyboard, date
and time, display, application information, and customer service.
2) "Home Page" module: mainly includes opening the lids, selecting the chambers,
selecting the amplification program, setting the parameters of the amplification
program, starting the experiment, and viewing and analyzing the experiment results.
3) "Amplification Programs" module: mainly includes creating new amplification program,
saving amplification program template, setting common amplification programs, and
finding and filtering amplification programs.
4) "Experimental Results" module: mainly contains experimental data analysis, upload,
copy and export, batch analysis, find and filter experimental data, and export reports.

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4.1 System introduction
1) System settings mainly include user manage, customer service, and system application.
Figure 4-1-1 System settings interface

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2) Login: After clicking User Login, you can directly log in using the preset admin account.
Figure 4-1-2 Login interface

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3) Customer service: You can scan the corresponding QR code to get the corresponding
after-sales service.
Figure 4-1-3 Customer service

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4.2 Home page
1) Homepage: click on the software to enter the home page, in the home page you can see
6 positions to choose from, each with 16 wells, multiple bays can be run simultaneously
(Figure 4-2-1).
Figure 4-2-1 Home page interface

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2) Start-up tip: the first time to enter the program will prompt to synchronize the network
and time. (Figure 4-2-2)
Figure 4-2-2 Start-up tip