Aquila Biolabs LIS User manual

LIS user guide
A handbook for automated feeding in shake
flask applications

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Table of contents
Introduction and general considerations ................................................... 4
The LIS-system –an overview............................................................... 6
Technical Specifications.........................................................................9
Recommended software requirements (LIS Software) ............................. 10
Recommended operating conditions ..................................................11
Warnings.................................................................................................12
Declarations and certificates................................................................14
CE conformity....................................................................................14
WEEE conformity..............................................................................15
FCC compliance...............................................................................16
Installation, quick start and handling........................................................ 17
Controlling the LIS Drive.......................................................................17
Main Menu Structure.............................................................................18
LIS Drive Menu Structure.....................................................................19
Running an LIS Experiment ............................................................................. 20
1. Auto Offset the Pressure........................................................20
2. Set up an experiment .............................................................20
3. Setting up the LIS Cartridge ..................................................22
4. Leakage Test ...................................................................................... 23
5. Prepare your LIS System.......................................................24
6. Assemble the Feed System...................................................25

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7. Start the Experiment...............................................................26
8. Sampling..................................................................................26
9. Stop the Experiment...............................................................27
Using a needle with big shake flasks...................................................27
LIS Software and Coordinator................................................................... 28
Introduction.............................................................................................28
Installation ..............................................................................................28
Starting and stopping the LIS Software...............................................28
Starting the LIS Software.................................................................28
Exiting the LIS Software...................................................................29
Using the LIS Software.........................................................................29
The user Interface.............................................................................29
Connecting the LIS Coordinator......................................................31
Connecting the LIS Drives...............................................................32
Running an Experiment ...................................................................33
Create a new Feeding Profile..........................................................36
Add new Parameters........................................................................37
Charging the LIS Drive..........................................................................38
Disinfection.............................................................................................38
Updating the LIS Drive Firmware.........................................................39
Troubleshooting Guide .........................................................................40
Contact....................................................................................................43

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Important aspect:
This kind of grey box will be used throughout the complete document
for the indication of important aspects, hints or summaries.
Introduction and general considerations
Welcome to the LIS user guide and congratulations for your decision of
choosing the LIS system to feed your shake flasks. The idea of this user guide is
to give you access to all information required to install, integrate and use the
LIS within your laboratory everyday life. Generally, it is strongly recommended
to read this user guide prior to any installation or operation works with the LIS
system.
Important aspects, hints or summaries are highlighted by grey boxes.
Caveats are indicated by warning signs.
Dangers are indicated by this type od sign
In order to ensure that this user guide provides you all information you need
during your work with the LIS, we as aquila biolabs are reliant on your feedback.
Do not hesitate to contact us to share your ideas regarding errors, missing
information or incomprehensibilities so that we can improve this document and
keep it up to date with your requirements.

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The latest manual is available on our homepage under
www.aquila-biolabs.de/service/downloads.
This user guide is still under development and should be recognized as
preliminary. This user guide, the LIS device and the LIS software may be
subjected to changes and improvements without further notice.
In case of any question that might arise during the work with LIS do not hesitate
tocontact us (Contact details on pp. 44).
LIS user guide revisions:
Revision 0
30.08.2017
- initial document
Revision 1
17.11.2017
- added troubleshooting guide, update
Revision 1.0.1
02.04.2018
- added LIS Software Guide, general
update

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The LIS-system –an overview
figure 1 LIS System
LIS stands for L iquid I njection S ystem. It allows automated feeding for shake
flasks. The LIS system consists of four different components, the LIS Drive, the
LIS Cartridge, the LIS Coordinator (available as add-on) and the LIS software
(available as add-on). Each component serves a different purpose. The LIS
Cartridge is a pre-sterilized, ready-to-use single use liquid container that holds
the liquid.
figure 2 LIS Cartridge

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The LIS Drive is the physical heart of the LIS-system, as it performs the
communication and dispensing tasks. It can be used in a stand-alone mode,
where the Drive is controlled via its display, or in a wireless mode. Here, several
Drives are connected to the LIS Coordinator through a 2.4 Ghz wireless
connection, which organizes all the communication traffic between the LIS
software and the Drives (only with software add-on).
figure 3 LIS Drive (left) and LIS Coordinator (right)
Furthermore, the LIS software is the user’s window to the world of automated
feeding in shake flasks fermentations. The LIS Software does all the visualization,
communication, and controlling works that is required in order to provide all
LIS users an intuitive and comfortable solution to prepare, run and store their
experiments.

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Figure 4 Profile generator of the LIS software

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Technical Specifications
LIS Drive
input voltage
input current
height (with mounting ring)
additional height on shake flask
5 VDC ± 5%
min. 200 mA
approx. 88 mm
approx. 70 mm
Temperature
operating temperature
charging temperature
Battery (built-in)
Type
Capacity
Runtime when fully charged
1
Charing time
Charging Source
15 –45 °C
15 –40°C
Li-Ion
300 mAh
about 1 week
approx. 2 h
USB 2.0 Port
Radio
Frequency
Range2
2.4 Ghz
upto 30 m
Pump
Flow rate
max. 1 ml/min
1
dependent on feeding profile
2
dependent on environment

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Cartridge
Sterilization Procedure Ethylene Oxide (EtO)
Fitting Flask Type straight neck (no brim)
Fitting Flask Outside Diameter 38 mm (±0.3 mm)
Fitting Flask Minimum Inside Diameter 33 mm
Recommended software requirements (LIS Software)
CPU current dual core @ 2 GHz/core or better
RAM / HDD
≥ 4 GB / ≥ 10 GB
Operating system Microsoft Windows 7 or newer with JRE 8
Screen resolution 1920 x 1080 pixels
Web browser Tested with Firefox V 54.0.1
It is strongly recommended to disable automated updates in the Windows
operation system settings, to prevent undesired system restarts upon Windows
Update events.
On Windows 10, a restart blocking service should be installed to prevent
system restarts in the night due toWindows Update.
It is recommended to use the “High performance” power plan in the Windows
power options.
You can also contact us to verify your chosen system settings.

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Recommended operating conditions
Temperature 10 –45 °C
Humidity (relative) 0 –80% (non-condensing)
Filling volume max. 25 ml
3
Tested Shaking speeds tested with fresh Infors clamps
shaking diameter ≤ 2.5 cm
100-125 ml shake flask max. 250 rpm
250-300 ml shake flasks max. 300 rpm
500-2000 ml shake flasks max. 250 rpm
shaking diameter ≤ 5.0 cm
100-300 ml shake flask max. 250 rpm
500-1000 ml shake flasks max. 225 rpm
2000 ml shake flasks max. 200 rpm
Note: values might be different when sticky
stuff is used or other addons are used (e.g.
the Cell Growth Quantifier by aquila
biolabs). Please carefully determine
acceptable shaking speeds with your setup.
3
Depending on shaking speed and diameter.

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Warnings
Do not use the LIS system or any of its components in
water bath shakers! This might result in electric shocks,
which could damage your health, the LIS and any other
electric device around.
Be careful when mounting the LIS cartridge onto the shake
flasks. Some shake flasks show a bigger neck diameter. Do
not try to force the cartridge onto such flasks as they could
break. Instead, useflasks that easily fit.
Be careful when handling the mounting rings. They might
have sharp edges that might cause injuries such as cuts.
Do not spill liquids over the LIS drive.
In general, do not use inorganic or organic acids and bases,
organic solvents or detergents to clean the LIS drive! Some
organic solvents or detergents might be allowed for
cleaning, but you should only use those being mentioned
in the user guide.
Always stop the shaker before doing anything with the LIS
components or the shake flasks. Especially for mounting
and dismounting purposes, the shaking movement should
be always turned off to avoid damage to your health and
tothe devices and flasks on the shaker.

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Any kind of opening, manipulating or copying LIS devices
as well as decompiling, reverse-engineering, copying or
distributing LIS software or the LIS firmware components
is strictly prohibited in accordance with German and
international law and may lead to compensation claims.
The LIS Drive contains a lithium ion battery. Take special
care considering following aspects:
- Do not dispose the battery / the LIS Drive into
normal waste.
- Do not heat or dispose the battery into fire, water
or other liquids.
- Do not put the Drive into a microwave, washing
machine or a drying machine.
- Never use a damaged battery.
- Never disassemble a LIS Drive.
- Should a battery unintentionally be crushed, thus
releasing its contents, rubber gloves must be used to
handle all battery components. Avoid inhalation of
any vapors that may be emitted.
- Take care when shipping the LIS Drive. Inform
yourself about shipping regulations of lithium ion
battery containing devices.
- Do not charge / discharge the battery outside the
operating temperaturerange (0-45°C)
- For longer storage of the LIS Drive, the battery
should be in a 50% charged state.

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Declarations and certificates
CE conformity
The aquila biolabs GmbH, Arnold-Sommerfeld-Ring 2, 52499 Baesweiler,
Germany, herewith declares under its sole responsibility that all devices and
equipment being part of the LIS system and being manufactured by the aquila
biolabs GmbH are in conformity with the Council Directives regarding
- Low Voltage Equipment Safety (2014/35/EU)
 evaluated on the basis of EN 61326-1:2013
- Electromagnetic Compatibility (2014/30/EU)
 evaluated on the basis of EN 61010-1:2010
- Restriction of certain Hazardous Substances (2011/65/EU)
 evaluated on the basis of EN 50581:2012.
This declaration applies to all products with the following identifiers:
LIS Drive
- LIS-DR-BV / LIS-DRIVE Revisions: 1.00
Technical documentation is maintained at the aquila biolabs GmbH headquarter
in Arnold-Sommerfeld-Ring 2, 52499 Baesweiler, Germany.
Date of declaration: 20.07.2017
Name, position of the undersigned: David Frank, Managing Director
Production

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WEEE conformity
WEEE-Registration-No.: 61144888
The aquila biolabs GmbH, Arnold-Sommerfeld-Ring 2, 52499 Baesweiler,
Germany, herewith declares compliance of all electronic components of the LIS
system with the Council Directive 2012/19/EU.
Electronic components may contain various hazardous substances that could
possibly exhibit negative impacts on your health and the environment. In order
to avoid those effects aquila biolabs encourages you to make use of the
appropriate local take-back and recycling systems for disposing electrical and
electronic equipment. By doing this you are furthermore significantly reducing
the pressure on natural resources and thus preserve our planet for subsequent
generations.
Components that are affected by this declaration carry the following pictogram:
Date of declaration: 20.07.2017
Name, position of the undersigned: David Frank, Managing Director
Production

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FCC compliance
FCC ID: VNR-ATSAMR210UA-0
This is a Class A product.
This device complies with Part 15 of the FCC Rules. Operation is subject to the
following two conditions:
(1) this device may not cause harmful interference, and
(2) this device must accept any interference received, including interference that
may cause undesired operation.
This equipment has been tested and found to comply with the limits for a Class
A digital device, pursuant to part 15 of the FCC Rules. These limits are designed
to provide reasonable protection against harmful interference when the
equipment is operated in a commercial environment. This equipment generates,
uses, and can radiate radio frequency energy and, if not installed and used in
accordance with the instruction manual, may cause harmful interference to radio
communications. Operation of this equipment in a residential area is likely to
cause harmful interference in which case the user will be required to correct
the interference at his own expense.

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Installation, quick start and handling
Controlling the LIS Drive
figure 5 controlling the Drive
• Make sure the transport security lock (piece of plastic below the
knob) is removed, otherwise the knob cannot be pushed
• Pressing and holding the knob for approx. 1 second turns on the
LIS Drive
• Turning the Knob left or right allows you to move in the menu
and/or increase and decrease numerical values
• A Single press on the knob selects an entry or confirms an input
• Pressing and holding the knob for more than 5 seconds triggers
a forced shutdown of the LIS Drive (only use this function if
problems occur)

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Main Menu Structure
• Status: Shows the experiment status if an experiment is running
• Control: Start or stop an experiment, shutdown the LIS Drive
o Leakage Test : test the assembly for leakage
o Estimate : estimate volume in cartridge (beta-function)
o Prepare: start the pressure regulation
o Start: start your experiment
o Stop: stop your experiment
o Manual Dispense : manually pump with the Drive
• Experiment: Setup of the dispensing protocol
• Settings: Change settings of the LIS Drive
• Shutdown: power off your LIS Drive

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Status
Status
Elapsed
Time
Remaining
Time
Dispensed
Volume
Remaining
Volume
Control
Leakage Test
Estimate
Prepare
Start
Stop
Sample
Manual
Dispense
Experiment
Profile
(Constant /
Multi /
Single /
Remote)
Repetitions
(only for
profile
Multi)
Dispense
Volume
Filling
Volume
Intital Delay
Total
Runtime
(not for
profile
Single)
Settings
Pressure
Press.(Pa)
Press.(Raw)
Equilib (Pa)
AutoOffset
Parameter
Pulse/10ul
P1 - P6
Step-size
Dwell-time
Wireless
Status
Zigbee Pan
ID
Reconnect
Details
Developer
Acceleration
Enter Boot
Do Crash
Test-Mode
RecoveryCo
unt
Battery (mV)
Battery (mA)
About
Shutdown
Cancel
Shutdown
LIS Drive Menu Structure
figure 6 Menu structure of the LIS Drive

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Running an LIS Experiment
1. Auto Offset the Pressure
Before using the LIS Drive the pressure sensor needs to be blanked. The Drive
has a function called “ AutoOffset ”to set the actual blank.
 Make sure no filter or cartridge is connected to the Drive
 Go to „Settings ”then „Pressure” and check if the value of the entry
„Pressure” is around 0 (+/- 5 counts)
 If the value differs, go to „AutoOffset” and press the knob
 Now the Pressure is blanked and the Drive is ready to use
2. Set up an experiment