ASI three 60 Operating and maintenance manual

INSTRUCTIONAL MANUAL
ASI
Life Sciences
Three60 Valve Instructions
Pinch and Cut Disconnect Instructions
Single-Use Aseptic Sampling System

THREE60 SAMPLING SYSTEM VALVE INSTRUCTIONS
PLEASE READ THESE INSTRUCTIONS CAREFULLY BEFORE USE
• Remove sample system from sterile
package.
• Apply a gasket, Three 60 unit and clamp
to angeinterfaceofyourvessel(1½”
sanitary connector or a NA port).
• Position valve with white depressible
tab pointing upward and the turn arrow
pointing clockwise for easy push and
turn operations.
• Conductyour“steaminplace”or“clean
in place”process.
Step 1: Installation of Unit
With the three60 system, there are no parts to be assembled,
disassembled or cleaned and unlike other single-use sampling systems,
no additional tools are needed. The entire set can be easily applied
toyouruidholdingvesselortransferlineandfoursamplescanbe
removed in only a few minutes.
three60iscompatibletoyourvesselwitheitheraNAportora1½”
sanitary connector; no expensive hardware is needed. Each three60
package contains a valve and four assemblies with Pinch and Cut
Disconnections.

THREE60 SAMPLING SYSTEM INSTRUCTIONS | 3
• When you’re ready to take a sample, slightly
depress tab feature and begin to turn main
body clockwise.
CAUTION: Excessive pressure on the tab may
cause damage and compromise the locking
action.
• Once the turn is initiated, release the tab;
the tab will lock when the open position is
reached. Immediately release the tab, as it will
lockwhentheopenowpositionisreached.
CAUTION: Holding tab as you turn may cause
turning past the ow opening.
• Fill product to the volume desired. For 250 ml
or heavier samples, support the bag with your
hand.
CAUTION: Observe the speed of lling to
avoid overlling.
Step 1: Installation of Unit
Step 2: Start Sampling


• Whensamplelliscomplete,pressthe
depressible tab and again grip and turn the
body clockwise.
• Again, release the tab immediately when
the turn is initiated; valve will lock in to place
when the next closed position is reached.
Step 3: Close Sampling

PINCH AND CUT DISCONNECTION
The Pinch and Cut Disconnection is the answer to the industry’s need
for a simple method of sealing and disconnecting a tube. With only the
operator’s hands, the device will pinch the tube closed, seal the tube
endsand“cut”themapart;thusenablingaquickdisconnectionofthe
sample bag.
PRE-USE DIRECTIONS
Check the clamps to ensure they are not pinching the tubing and
cuttingofftheowpathbygentlypullingeachclampupward,butnot
disconnectingthem.ThePinchandCutisnowreadyforuidtransfer.
PINCH AND CUT DIRECTIONS
1) First,aftertheuidtransferiscomplete,usingbothhands,gripthe
cutting clamp closest to the sample bag and press down until the audible
click is heard and the top rests on the lip.
NOTE: It is CRUCIAL that the clamp closest to the sample bag is
engaged rst.
2) Repeat the same action as step one with the clamp closest to the valve.
3) When you are sure the lock has engaged, grip the clamps with both
hands and the “V” facing you.
4) Press thumbs towards each other. This action will break the clamps to
allow separation. Continue the motion until the tube separates. The clamp
is permanently closed and integrally sealed.

THREE60 SAMPLING SYSTEM INSTRUCTIONS | 5
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4.

THREE 60 SINGLE-USE ASEPTIC SAMPLING SYSTEM
TAKING A SAMPLE MADE TO BE SIMPLE
The simple design behind the three60 Sampling System allows you to take a
representative sample of your product with minimal effort.
For a completely sterile transfer, use a CIP or SIP process to prepare your tank.
Thepre-irradiatedbagsandassemblieswillensureasterileuidpathwhile
thequick-turnvalveandPinchandCutDisconnectionsmaintainsteriletransfer
and removal.
SINGLE-USE SOLUTIONS
Single-use systems are providing improved sterility and reduced cleaning
requirementsovertraditionalsamplingsystemsacrossallapplicationsinthe
Bio-Pharmaceutical Industry.
The three60 Single-Use System brings the same advantages to sampling
resultinginaquickersolutionandasaferprocess.
EFFICIENT
With the three60 system, there are no parts to be assembled, disassembled
or cleaned and unlike other single-use sampling systems, no additional tools
areneeded.Theentiresetcanbeeasilyappliedtoyouruidholdingvesselor
transfer line and four samples can be removed in only a few minutes.
three60iscompatibletoyourvesselwitheitheraNAportora1½”sanitary
connector; no expensive hardware is needed. Each three60 package contains a
valve and four assemblies with Pinch and Cut Disconnections.
ASI |163ResearchLane,Millersburg,PA|(717)692-2104|(800)724-4158|www.asisus.com
ASIisanFDARegistered,ISO13485:2003CertiedManufacturer
RevC01-17-2013PK-01195
ASI
Life Sciences