ATAGO RePo-1 User manual

5010-E09
Portable Refracto-Polarimeter
Instruction Manual
Cat. No. 5010

クイックマニュアル
3 Measurement and Evaluation
② (rpage 6・page 7)
①Wipe the sample stage clean.
②Fill the sample stage with the sample
②
solution up to the fill line.
③Press F.
The measurement value is displayed. The
green/red indicators notify whether the
measurement falls within or outside the
user-programmed tolerance setting. (rpage 7)
N
See page 8 for cleaning tips.
N Seepage 9forhowtochangethesettings.
Quick Manual
1
Powering on
(rpage 4)
①Press I.
2Zero setting (rpage 5)
①Wipe the sample stage clean.
②Fill the sample stage with water up to the
fill
②
line.
③Press G.
③ is displayed.
The cursor indicates the last used
scale.
Sample volume
(約
Fill line
Measurement
evaluation indicator
(Approx. 3mL)

Multi
-
scale: Angle of Rotation; Brix; Purity; International Sugar Scale;
Specific Rotation; Concentration
Main Features
Dual
-
purpose: Refractive Index (Brix) and Angle of Rotation Measurements
旋光度
+
屈折率
(Brix)
Angle of Rotation

%
Brix (%)
% %
Measurement Evaluation Indicator
The measurement values are evaluated against the pre-set tolerance, and the
green/red light will indicate the results.
See page 10 for how to configure upper and lower limits.
Red light = Outside tolerance
Green light = Within tolerance
Lower limit Upper limit
Outside tolerance Within tolerance Outside tolerance
Red light Green light Red light
%
Refractive
Index
(Brix)
Press Hto select the desired
scale – International Sugar Scale;
Purity (factory default);
Specific Rotation; or
Concentration
Angle of
Rotation

Substances that could
not be measured until now:
A multi-component solution.
A + B + C
Main Features
Example: Concentration (Brix) and Angle of Rotation

Purity
Concentration and angle of rotation of sugar cane juice
sucrose content (purity)
Can the purity scale be used to determine the concentration of one solute in a
solution with multiple solutes? How about for my unique application?
For further information, please contact ATAGO!
a
A fusion of index of refraction and angle of optical rotation -Making the invisible, visible-
The One and Only Polarimeter That Doesn’t Use Observation Tubes
The instrument measures angle of rotation equivalent to a 20mm observation tube.
Conversions to other observation tube lengths can be done by calculation.
Instrument’s displayed angle of rotation × (Length of observation tube you wish to
convert ÷20mm) ＝Converted observation tube’s angle of rotation
Example:
When the instrument’s displayed angle of rotation is －2°:
Conversion to 100mm observation tube: －2°×（100÷20）＝－10°
Conversion to 200mm observation tube: －2°×（200÷20）＝－20°
For cases when your objective is, “I want to know
the concentration of B!”
Please try it out. ATAGO appreciates your
Multiple
components
Det
ermine the
concentration of
one component
It may be possible
to measure these
substances.
Multi-component
sucrose solution
Sucrose + other substances
a
a
Measure with RePo-1
Brix (Concentration)
Angle of Rotation
Determine the
amount of
sucrose

Useful Features
Adjust Auto Shut
-
off
Timer
(rpage 12)
Turn on Backlight
Backlight stays on for 1 minute after any button is pressed.
View Scale Information
Change Scale
Is the unit turning itself off too soon?
g
Choose from timer options of 4, 10, or
30 minutes.
During measurement: Press START to display the scale name abbreviation
next to the
battery indicator.
After measurement: The cursor indicates the last used scale.


℃


℃



℃


℃


℃


℃
Angle of
Bri
x (%)
Purity
International
Specific
Concentration
Rotation
Sugar Scale
Rotation
Angle of Rotation Brix (%) User (Purity, International Sugar Scale,
User (Specific Rotation, or Concentration)

% % %
Press the desired scale button.
J: Angle of Rotation
K: Brix (%)
H: Every time H is pressed,theotherscalescyclethroughin thefollowing order:
Purity→International Sugar Scale→Specific Rotation→Concentration
F
%
%
%
%

Then, the measurement in the selected scale is displayed.
After measurement: Press one of the scale buttons to di
splay the following scale name abbreviation next to
the battery indicator.
% % %



Contents
Precautions for use ················································································································· 1
1. Overview·································································································································· 2
1. Contents ········································································································································································· 2
2. Components and Functions ································································································································· 2
3. Battery Replacement ··············································································································································· 4
4. Power ON/OFF ·························································································································································· 4
2. Basic Operations ················································································································· 5
1. Zero-setting ································································································································································· 5
2. Measurements ····························································································································································· 6
3. Cleaning ··································································································································· 8
4. Settings ···································································································································· 9
How to Access Settings·············································································································································· 9
1. Tolerance Setting ···················································································································································· 10
2. Concentration/Specific Rotation Setting ··································································································· 11
3. Auto Shutdown Timer Setting ·························································································································· 12
5. Scales ····································································································································· 13
6. Error Messages ·················································································································· 14
7. Inspection······························································································································ 15
8. Storage and Maintenance ····························································································· 15
9. Optional Accessories and Consumable Items ····················································· 16
10. Warranty and Repair ······································································································ 17
1. Warranty ······································································································································································· 17
2. Replacement Parts ················································································································································· 17
11. Specifications ··················································································································· 18

-1-
Precautions for use
Introduction
Thank you for purchasing the instrument. Carefully read and follow all instructions. Keep this
manual for future reference.
Safety Instructions
Read and follow all safety instructions before operating the instrument.
Failure to comply with the following instructions may result in personal injury or property damage.
 WARNING
Ensure safety when handling hazardous materials.
Observe precautionary measures and use protective
equipment, Be aware of the hazards of such
chemicals
and emergency response guidelines.
Do not drop the instrument or subject it to strong
physical shock.
ATAGO may not be held liable for any injury or
damage
arising in connection with handling of hazardous
materials during the use of the instrument.
Do not attempt to repair, modify, or disassemble the
instrument.
 CAUTION
Carefully read this manual to have basic knowledge
of
the function of each component.
Some acids may corrode the glass prism, optical
path window, and/or (metal) sample stage, which
may cause
erroneous measurements.
Do not use metal tools, such as a spoon, as they
may
scratch the prism or the optical path window,
resulting in erroneous measurements.
Only use the specified battery type. Observe proper
polarities, properly aligning the anodes and
cathodes.
Store the instrument away from direct sunlight/heat
sources and excessive amounts of dust/debris.
Store the instrument in a dry environment.
Do not expose the instrument to a rapid change in
ambient temperature.
Do not subject the instrument to strong vibration.
Do not subject the instrument to extreme cold
temperature.
Do not place the instrument under anything heavy.
Have the batteries and battery compartment cover
removed during air transport.
〈Chemical Resistance of Body Case〉
The body case is made of ABS resin. Do not expose it to water vapor or solvents.
Aqua regia / chromic acid / chlorosulfuric acid / hydrobromic acid / nitric acid / hydrofluoric acid / sulfuric acid / phosphoric acid / ethyl
acrylate / butyl acrylate / ethylacetoacetate / acetophenone / benzyl benzoate / ethylbenzene / ethylene oxide / ethylenediamine /
ethylene chlorohydrin / epichlorohydrin / ethyl chloride / benzyl chloride / methyl chloride / chlorinated solvents / xylene (xylol) / cresol
/ chloroacetone / chlorotoluene / chloroform / acetic acid / amyl acetate / isopropyl acetate / ethyl acetate / butyl acetate / propyl
acetate / methyl acetate / diphenyl oxide / diisopropyl ketone / carbon tetrachloride / dioxane / cyclohexanone (anone) /
dichlorobenzene / dibutyl phthalate (DBP) / dimethylformamide (DMF) / dimethylaniline / phenol / thioalcohol (mercaptan) /
tetrahydrofuran (THF) / trichloroethylene / toluene (toluol) / ethylene dichloride / dichloromethane / nitroethane / nitropropane /
nitrobenzene / nitromethane / perchlorethylene / fluorobenzene / methyl methacrylate / methyl isobutyl ketone / methyl ethyl ketone /
monochloroacetic acid / monochlorobenzene / liquid chlorine / thionyl chloride / sodium peroxide / bromine / benzene / lacquer
m Solvents that are harmful to the plastic body case include but are not limited to the above substances.

-2-
1. Overview
1. Contents
・ Main unit··································· 1 ・ AAAbatteries·····························4
・ Instruction Manual (this book) ······· 1 ・Disposable plastic pipettes············ 3
2. Components and Functions
START button (F)
Take a measurement.
Select a setting item.
Measurement modes =
Settings =
POWER button (I)
Turn on in the measurement mode.
LCD
Displays the measurement values,
temperature/scale, and battery indicator.
Sample stage
Apply the sample to be measured.
Prism
Measures the Brix (%).
Fill line
Indicates a sufficient
amount of liquid.
Optical path window
Polarized light is
emitted and received.
Measurement Evaluation Indicator
Either the green or red light comes on
when the measurement value is within or
outside the tolerance range respectively.
Scale button
ZERO button ( G )
ZERO set.
Confirm the selection.
A.R. button (
J)
Select the angle of rotation scale.
Move to the next place value.
Brix button (K)
Select the Brix (%) scale.
Increase the value.
User button (H)
Select from : Purity; International Sugar Scale;
Specific Rotation; or Concentration
Decrease the value.

-3-
LCD
Bottom view
Battery compartment
Place and remove batteries
from here.(rpage 4)
Temperature/Scale
The scale, and then, the temperature is displayed
for each measurement.
％
Cursor
The cursor indicates the last used scale.
Scales
A.R.
°: Angle of Rotation
Brix : Brix (%)
User : User (Purity,
International Sugar Scale,
Specific Rotation,
or Concentration)
Measurement value
Battery indicator



-4-
3. Battery Replacement
 CAUTION
●
When the battery icon indicates the low power level ( ), replace all four
batteries with a brand new set of AAAalkaline batteries (1.5V).
●
Check the expiration dates on batteries before purchase.
●
Remove the battery from the unit when the unit is not to be used for a month
or so.
①Turn the screw counterclockwise
with a flat-head screwdriver or a
coin to open the battery
compartment.
②Insert the batteries, observing
the correct polarity.
③Close the battery compartment
and turn the screw clockwise.
4. Power ON/OFF
Powering on
①Press I.
Turn the instrument on in the measurement mode.
The backlight stays lit for 1 minute.
The cursor indicates the last used scale.
N The backlight stays on for 1 minute after any
button is pressed.
N To access the settings, while the power is turned
on, press and hold “user”, then 1 second later,
press “Power” (
r
page 9).
Powering off
①Hold down I.
The screen will turn off.
N The instrument will turn itself off after 4 minutes of
inactivity by default.
The auto shutdown timer can be extended to 10
minutes or 30 minutes (
r
page 12).
g
g
Open Close

-5-
2. Basic Operations
 CAUTION
●
Operate away from direct sunlight at a stable temperature with as little
fluctuation as possible.
●
Acclimate both the instrument and sample solution to room temperature to
ensure accurate results. Measurements may fluctuate with hot or cold samples.
Wait to press
F
after placing the sample on the prism.Alternatively, press
F
multiple times.
●
Operate on a flat work surface.
●
The displayed temperature is that of the prism and may not necessarily match
the temperature of the sample.
1. Zero-setting
This is the operation to set the calibration point (zero) for optical rotation and Brix.
m Zero-set the instrument at the beginning of each day before use.
m Regular zero-setting throughout the day is recommended to ensure accurate results.
①Press I to turn it on.
m Optical rotations and Brix can be
zero-set in any scale, however, when
the optical rotation scale is selected,
even when Brix zero-set is an error, no
error message will display.
② Wipe the sample stage clean.
③ Fill the sample stage with tap water up
to the fill line.
N Use room temperature tap water. The
instrument should also be acclimated
to room temperature prior to
operations.
④ Press G.
 flashes and stops.
N
To perform zero-setting on the optical
rotation scale and check whether Brix
zero-setting was done correctly, press
Kand check that 000 is displayed.
Fill line
(Approx. 3mL)

-6-
2. Measurements
①Wipe the sample stage clean.
②Fill the sample stage with the sample
solution to the fill line.
③Press F.
---flashes, and then the scale name is
displayed next to the battery indicator.
Then, the measurement value and
temperature will be displayed.
N Measure room temperature (distilled)
samples. The instrument should also
be acclimated to room temperature
prior to operations.
N Try stirring the sample on the sensor
during measurement to improve the
repeatability of oily/fatty samples.
m The measurement evaluation indicator
will light up if a tolerance is set for the
scale used (
r
page 10).
m The indicator will not activate for
erroneous results (including
temperature errors).
m
Set the upper and lower limits to 00.0 to
turn off the measurement evaluation
indicator.
Fill line
(Approx. 3mL)

-7-
90.0
Sample A Sample B Sample C
90.0% 100% 108%
g
ggg
Red light
Green light
Red light
Sample: Sucrose solution (26.0g/100mL)
+95.0% to +105%
Outside tolerance Within tolerance Outside tolerance
Red light Green light Red light
Outside tolerance
90.0%
Sample A
Within tolerance
100%
Sample B
Outside the tolerance
108%
Sample C
Red/Green Light Indication
〈Measurement Evaluation Indicator〉
Example: When the Purity scale is configured with a lower limit of 95.0% and an upper limit of 105%.
①Select the Purity scale.
②Press F to take a measurement.
--- will appear.

-8-
3. Cleaning
 CAUTION
●
Ensure that the sample stage is clean before measurement.
●
Clean the sample stage immediately after each measurement.
●
Thoroughly dry the instrument before storing.
●
Do not use benzene (paint thinner) on the instrument.
●
Store away from direct sunlight at a stable temperature with as little
fluctuation as possible.
①Collect the sample
solution with a pipette
and wipe it off.
③Dry the sample stage
completely with dry
tissues.
Ethyl
alcohol
Mild soap
For oily samples
Clean oily residues with
ethyl alcohol or mild soap,
and then, rinse with water.
②Apply some water and
wipe it off.
a
a
N Clean the prism
and the optical path
window particularly
well.
Prism
Optical path window
Can be washed under running
water.
Thoroughly dry the instrument
before storing.
N
Moisture may accumulate in
the grooves of the buttons, but
this will not affect the
instrument.

-9-
4. Settings
How to Access Settings
This section will explain how to change settings after accessing the menu.
G
F
G
F
G
F
G
F




































Exit the settings. Return to the measurement mode.
②Press F to
move to the next
setting item.
③Press G to select the setting.
1. Tolerance Setting (
r
page 10)
2. Concentration/Specific Rotation
Setting (rpage 11)
3. Auto Shutdown Timer Setting
(rpage 12)
Return to   .
①While the power is
turned on, press
and hold “user”,
then 1 second
later, press
“Power”.
H
↓
after 1 second
H＋I

-10-
1. Tolerance Setting
Set upper and lower limits fora scale,and the indicator will light up to notify if a measurement falls within or outside thetolerance.
Range: -999 to +999
Resolution
(regardless of positive or negative value) :
>100 : 1
10.0 to 99.9 : 0.1
<9.99 : 0.01
[Factory default: lower limit = -5.00，upper limit = +5.00 in Angle of Rotation]
G
Confirm the scale.
G
Confirm the upper limit.
Howto access the setting (rpage 9)








G
F






































F
F
Angle of Rotation
Brix (%)
Concentration Specific Rotation
F
F
F




















Purity
International Sugar Scale
①J→While ⑴is flashing
aSelect the sign by Kor H.
②J→While ⑵is flashing
aIncrease the value by K .
aDecrease the value by H .
③J→While ⑶is flashing aChoose to insert a decimalpoint by
Kor H .
④J→While ⑷is flashing aSame as ②above.
⑤J→While ⑸is flashing aSame as ③above.
⑥J→While ⑹is flashing aSame as ②above.
⑵Value









Enter the upper limit.
⑹Value
⑸
Decimal
⑸
point
⑷Value
⑶
Decimal
⑸
point









Enter the lower limit.









G
Return to the measurement mode.
G
Confirm the lower limit. “” will flash on the display.
F








Exit the setting.
N Press Gto continue to edlit
the setting.
m A tolerance can be set for no
more than 1 scale at a time.
N The tolerance range is different
from the display range.
m Set the upper and lower limits to
0.00 to turn off the measurement
evaluation indicator.
N The last used scale will be
selected upon returning to the
measurement mode.
⑴ Sign
N
When cancelling settings, press
F
.
Proceed
to lower limit value input.
N When cancelling settings,
press F .

-11-
2. Concentration/Specific Rotation Setting
Range: Concentration 0.00 to 999
Range: Specific Rotation -999 to +999
Resolution
(regardless of positive or negative value) :
>100 : 1
10.0 to 99.9 : 0.1
<9.99 : 0.01










G
Confirm the scale.
Howto access the setting (rpage 9)









G
①J→While ⑴is flashing
aSelect the sign by Kor H .
②J→While ⑵is flashing aIncrease the value by K .
aDecrease the value by H .
③J→While ⑶is flashing aChoose to insert a decimalpoint by
K or H .
④J→While ⑷is flashing aSame as ②above.
⑤J→While ⑸is flashing
aSame as ③above.
⑥J→While ⑹is flashing aSame as ②above.
⑵Value
Enter the value.
⑹Value
⑸
Decimal
⑸
point
⑷Value
⑶
Decimal
⑸
point









G
Return to the measurement mode.
G
Confirm the value. “” will flash on the display.
F









Exit the setting.
N Press G to continue to edit
the setting.
To measure Concentration a
enter Specific Rotation
To measure Specific Rotation a
enter Concentration
N Both variables can be configured.
N The last used scale will be
selected upon returning to the
measurement mode.
⑴ Sign
[Factorydefault: Concentration of 26.0g/
100mL，Specific Rotation of 66.6°]
Concentration Specific Rotation
F


















N When cancelling settings,
press F .

-12-
3. Auto Shutdown Timer Setting
Choose from the following 3 timer options.
F
G
Howto access the setting (rpage 9)









[Factory default: 4 minutes]
G
Confirm the selection. “” will flash on the display.


















Turn off after 4 minutes of inactivity
G
F


























F
Turn off after 10
minutes of inactivity
F
Exit the setting.
N The last used scale will be
selected upon returning to the
measurement mode.
Turn off after 30
minutes of inactivity
N Press Gto continue to edit
the setting.
Return to the measurement mode.

-13-
5. Scales
ANGLE OF ROTATION - Denoted by "α" and expressed in degrees ("°")
The rotation of polarized light is observed when polarized light that is oscillating unidirectionally rotates as it
passes through a liquid. The measurement of the angle of such rotation is called the "Angle of Rotation."
The angle of rotation is the basic numeric value measured by polarimeters and are dependent on the
concentration of the liquid, the length of the observation tube, temperature, and the measurement wavelength.
The instrument measures angle of rotation equivalent to a 20mm observation tube.
Brix (%)
Brix (%) represents the weight of sucrose in 100 grams of sucrose solution as percentage by weight. When other
dissolved solids are present in the solution, Brix (%) conversion may be applied.
Brix (%) is a measure of the total dissolved solids in a solution and indicates the combined concentration of all
soluble substances, such as sugar, salt, protein, and acids.
The readings are corrected, based on the temperature of the prism, within the automatic temperature
compensation range.
PURITY - Expressed in %
The Purityis the proportion of sucrose comprised in a soluble solid content (a sample).
Sucrose content is symbolized by "POL," and is determined by the following formula:
POL = (26.016/Mass (density) of 100mL) × International Sugar Scale
Themass (density) of 100mL is determinedbytheBrix(%)valuethathasbeen input into theinstrument beforehand.
The purityis determined by the sucrose content and the Brix (%) value, using the following formula:
Purity = (POL/Brix) × 100
The instrument displays temperature-compensated purity, based on the angle of rotation and Brix measurements.
Purity readings are always positive, but negative values are also displayed when the angle of rotation is negative.
INTERNATIONAL SUGAR SCALE (ISS) - Expressed in °Z
The International Sugar Scale is determined by the following formula:
International Sugar Scale = Angle of Rotation × 2.888
The measurement value of 26.000g/100cm
3
of sucrose solution in a 200mm observation tube is 100°Z.
This measurement value is commonly used in the sugar manufacturing industry and is usually
temperature-compensated to the reference temperature of 20˚Cor 68˚F.
The instrument multiplies the angle of rotation readings with the 20mm observation tube by 10 (to calculate the
equivalent readings with a 200mm observation tube) to compute the °Z values.
SPECIFIC ROTATION - Denoted by "[α]" and expressed in degrees ("°")
The Specific Rotation is a numeric value unique to each substance. By definition, the specific rotation value is
equivalent to the angle of rotation obtained by a measurement using a solution of 100% concentration in a
100mm observation tube (although such a measurement is actually not practicable). The specific rotation is
determined by the following formula:
Specific Rotation = (10,000 ×Angle of Rotation)/(length of observation tube [mm] × Concentration [g/100mL]
*1
)
*1
m To measure the specific rotation, input the concentration value of the
※1
m sample to be measured into the instrument before taking a measurement. (
r
page 11)
CONCENTRATION - Expressed in g/100mL
The Concentration is the density of an optically active substance dissolved in water or alcohol. The concentration
is determined bythe following formula:
Concentration = (10,000 × Angle of Rotation)/(length of observation tube [mm] ×
Specific Rotation
*2
)
*2
m To measure the concentration, input the specific rotation value of the
※2
m sample to be measured into the instrument before taking a measurement. (
r
page 11)