Azure Biosystems chemiSOLO User manual

chemiSOLO
User Manual
Part Number CS1000

chemiSOLO User Manual Page 2
Safety and Regulatory Compliance
Important Safety Information
Please read these instructions before operating the chemiSOLO.
Electrical Safety Precautions
Be sure to take proper precautions when handling any electrical equipment. NEVER work on any live
circuit, xture, receptacle, or switch. Safety rules you should follow whenever working with any electrical
appliance include:
• Always shut off power at the main disconnect before changing a fuse.
• Always shut off power to the circuit before repairing or replacing a switch, receptacle, or xture.
• Always tape over the main switch, empty fuse socket, or circuit breaker you are working on.
• Always check that the circuit is dead before beginning work on it. Using a circuit tester or voltmeter
can help you determine this.
• Always unplug any appliance before repairing it.
Protective ground terminal
The ground terminal, intended for connection to an external protective conductor for protection
against electric shock in case of a fault, is located on the inside of the back panel.
For Research Use Only
This instrument is suitable for research use only. It must be used, therefore, only by personnel who know the
health risks associated with the reagents that are normally used with this instrument.
Warranty
The chemiSOLO is warranted against defects in materials and workmanship for one year unless otherwise
outlined on your sales order or www.azurebiosystems.com/warranty. If any defect occurs in the instrument
during this warranty period, Azure Biosystems, Inc. will repair or replace the defective parts at its discretion
without charge. The following defects, however, are specically excluded:
• Defects caused by improper operation.
• Repair or modication done by anyone other than Azure Biosystems or the company’s
authorized agent.
• Use of spare parts supplied by anyone other than Azure Biosystems.
• Damage caused by accident or misuse.
• Damage caused by disaster.
• Corrosion caused by improper solvents or samples.
• Unauthorized movement of the chemiSOLO.

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Voltage Setting Information
The chemiSOLO has a power supply that automatically chooses the correct voltage for your country or region.
CE Conformity
The chemiSOLO is in conformity with the provisions of the following EC Directives and the following
harmonized standards have been applied:
• EN61010-1: 2012
• EN IEC 62311:2020
• EN 301489-1 V 2.2.3:2019
• EN301489-17 V 3.2.4:2020
• EN IEC 61000-3-2:2019+A1
• EN 61000-3-3:2013+A1
• EN IEC 61326-1:2021
• EN 300328 V 2.2.2:2019
FCC Part 15, Subpart B:2020
Contact
Azure Biosystems, Inc.
6747 Sierra Court, Suite A-B • Dublin, CA 94568 • USA
[e-mail] • [phone] • Fax [phone]

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Table of Contents
1. Introduction 5
1.1 Table of Specications 5
1.2 Contacting Technical Support 5
2. Installation and Setup 6
2.1 System Placement 6
2.2 Connecting to Power 6
2.3 Turning the System On/Off 6
2.4 Software Access 7
2.5 External Wi-Fi Setup (optional) 9
3. Image Capture Overview 11
3.1 Home Screen Overview 11
3.2 Chemiluminescent Imaging 12
3.3 Visible Imaging 12
3.4 Custom Imaging 13
3.5 Auto-Save 14
4. Image Gallery Overview 15
4.1 Gallery 15
4.2 Image Adjustment 16
5. Settings Overview 18
5.1 General 18
5.2 Software 19
5.3 Create Darkmasters, Create Flats 20

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1. Introduction
The chemiSOLO offers high performance chemiluminescent and visible imaging in a compact design with
unique external control methods. Each chemiSOLO instrument includes the following key components:
• Body – The body is a light-tight imaging station, containing the camera and lens, mirrors, electrical
components, Ethernet, Wi-Fi emitter, and USB ports for data collection and external computer control.
• Stage – The imaging stage is a user-accessible, transparent platform for sample placement just
beneath the inner lid, which contains the LED light sources.
• Camera – High resolution 6.29MPx camera, preinstalled in the system.
• LEDs – The LED module allows for visible imaging, utilized for chemi marker images and visible
stained gel images in both color or grayscale.
• USB Ports – There are two USB ports for data transfer or for the external Wi-Fi dongle [CS1006], both
located at the back of the instrument.
• Ethernet Port – There is one ethernet port on the back of the instrument, which enables direct
computer connection with the use of an ethernet cord [CS1007].
• USB Memory Stick – The Azure Biosystems memory stick contains calibration les relevant to your
chemiSOLO system.
1.1—Table of Specications
Image Resolution 6.29M pixels
Maximum Field of View 10.0 x 15.0cm (4.0 x 5.9in)
Image Output 16-bit tiff, 8-bit jpg
Working Environment Ambient temperature: 10–30 °C
Humidity: up to 80%
Power Requirement 100–240VAC, 1A
Dimensions (W x H x D) 29.2 x 22.2 x 43.2cm (11.5 x 8.75 x 17.0in)
Weight 9.1kg (20.0lbs)
1.2—Contacting Technical Support
For questions regarding installation, setup, or general use of the chemiSOLO, please contact
[e-mail] or call [phone], 9am–4pm PST (GMT-8).

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2.1—System Placement
As with all electrical instruments, the chemiSOLO should be located away from water, solvents, or corrosive
materials on a at and stable surface with adequate clearance on all sides. The system must remain
stationary during operation.
The system should be placed away from interfering electrical signals and magnetic elds. If possible, a
dedicated electrical outlet should be used to eliminate electrical interference from other instrumentation in
your laboratory.
The chemiSOLO should be installed at no more than 3,000 meters above sea level. The unit should be placed
out of direct, bright sunlight or illumination sources to guard against light leaks.
2.2—Connecting to Power
The power entry module is located in the lower right on the back panel of the system. Connect the power cord
to a secure power outlet.
It is important to connect the system to a well-grounded power source. Azure Biosystems recommends that
you employ a surge-protecting power strip to protect against unexpected power surges that may cause
damage to the electrical components.
2. Installation and Setup
2.3—Turning the System On/Off
The main power switch is located on the back panel, just above the power entry module.
1. First, ip the power switch to On (I).
2. The light on the front of the chemiSOLO will turn blue and white while loading, then green once the
instrument is ready for use.
To turn off the system, shut down the chemiSOLO by:
1. Disconnecting from the chemiSOLO software on the controlling device.
2. Then, shut off power to the system by ipping the power switch on the back of the instrument to
Off (O).

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2.4—Software Access
The chemiSOLO Capture Software uses a unique, web-based browser that connects through either a direct
ethernet connection or through a Wi-Fi signal to an external device.
Ethernet Connection
To establish a connection to a chemiSOLO unit through ethernet, connect an ethernet cord to the ethernet
plug located in the middle-bottom of the back of the instrument while the unit is powered off. Then, connect
the other side of the ethernet cord to an external computer. Power on the chemiSOLO and wait until the
light on the front turns green. From there there, open a web browser on the computer (Google Chrome
recommended) and input the chemiSOLO’s ethernet IP address “192.168.40.1” to access the chemiSOLO
control software.
Wi-Fi Connection
Alternatively, each chemiSOLO can be controlled by connecting to its unique Wi-Fi signal and accessing the
chemiSOLO control software through a web browser. As such, it can be controlled by computers, tablets,
or mobile devices. For directions on connecting your particular device via Wi-Fi, please nd the appropriate
section below.
Connecting a PC to the chemiSOLO
• PC System Requirements:
• 2GB RAM or up
• Windows 10 or up
• Ethernet-compatible or Wi-Fi-enabled
• Must have a web browser installed (Google Chrome recommended)
1. Select the Network icon on the taskbar. If you do not see the Network icon or Disconnected icon,
select the Up arrow to nd the icon.
2. (Windows 11) Access the Manage Wi-Fi connections menu using the right arrow (>).
3. Select the Wi-Fi network specic to the desired chemiSOLO unit and select Connect. If a custom
password was set previously, input the password and select Connect.
Note: The default settings for the system’s unique Wi-Fi system will be “chemiSOLO_[system serial
number]” with no password. For reference, each unit’s serial number can be located on the back of
the instrument.
4. Once the PC is properly connected to the chemiSOLO’s Wi-Fi, open a web browser and input the
chemiSOLO’s Wi-Fi IP address “192.168.12.1” to access the control software.
Connecting a Mac to the chemiSOLO
• Mac System Requirements:
• 2GB RAM or up
• macOS 10.12 “Sierra” or up
• Ethernet-compatible or Wi-Fi-enabled
• Must have a web browser installed (Safari recommended)
1. Select the Wi-Fi icon in the menu bar.
2. Select the Wi-Fi network specic to the desired chemiSOLO unit and click Join. If a custom password
was set previously, input the password and select Join.
Note: The default settings for the system’s unique Wi-Fi system will be “chemiSOLO_[system serial
number]” with no password. For reference, each unit’s serial number can be located on the back of
the instrument.

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3. Once the Mac is properly connected to the chemiSOLO’s Wi-Fi, open a web browser and input the
chemiSOLO’s Wi-Fi IP address “192.168.12.1” to access the control software.
Connecting an Android Phone to the chemiSOLO
• Android Phone System Requirements:
• Android 8.0 “Oreo” or up
• Ethernet-compatible or Wi-Fi-enabled
• Must have a web browser installed (Google Chrome recommended)
1. Open the Settings app.
2. Navigate to Wi-Fi menu.
3. Select the Wi-Fi network specic to the desired chemiSOLO unit and select Connect. If a custom
password was set previously, input the password and select Connect.
Note: The default settings for the system’s unique Wi-Fi system will be “chemiSOLO_[system serial
number]” with no password. For reference, each unit’s serial number can be located on the back of
the instrument.
4. Once the Android phone is connected to the chemiSOLO’s Wi-Fi, open a web browser and input the
chemiSOLO’s Wi-Fi IP address “192.168.12.1” to access the control software or alternatively, scan the
QR code on the inner lid of the unit to quickly access the same address.
Connecting an iPhone to the chemiSOLO
• iPhone System Requirements:
• iPhone 8 or up
• iOS 12 or up
• Ethernet-compatible or Wi-Fi-enabled
• Must have a web browser installed (Safari recommended)
1. From the Home screen, open the Settings app.
2. Navigate to the Wi-Fi menu.
3. Select the Wi-Fi network specic to the desired chemiSOLO unit. If a custom password was set
previously, input the password and select Join.
Note: The default settings for the system’s unique Wi-Fi system will be “chemiSOLO_[system serial
number]” with no password. For reference, each unit’s serial number can be located on the back of
the instrument.
4. Once the iPhone is connected to the chemiSOLO’s Wi-Fi, open a web browser and input the
chemiSOLO’s Wi-Fi IP address “192.168.12.1” to access the control software or alternatively, scan the
QR code on the inner lid of the unit to quickly access the same address.
Connecting an Android Tablet to the chemiSOLO
• Android Tablet System Requirements:
• Android 8.0 “Oreo” or up
• Ethernet-compatible or Wi-Fi-enabled
• Must have a web browser installed (Google Chrome recommended)
1. Open the Settings app.
2. Navigate to the Wi-Fi menu.

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3. Select the Wi-Fi network specic to the desired chemiSOLO unit and select Connect. If a custom
password was set previously, input the password and select Connect.
Note: The default settings for the system’s unique Wi-Fi system will be “chemiSOLO_[system serial
number]” with no password. For reference, each unit’s serial number can be located on the back of
the instrument.
4. Once the Android tablet is connected to the chemiSOLO’s Wi-Fi, open a web browser and input the
chemiSOLO’s Wi-Fi IP address “192.168.12.1” to access the control software or alternatively, scan the
QR code on the inner lid of the unit to quickly access the same address.
Connecting an iPad to the chemiSOLO
• iPad System Requirements:
• iPad (5th generation) or up
• iPadOS 13 or up
• Ethernet-compatible or Wi-Fi-enabled
• Must have a web browser installed (Safari recommended)
1. From the Home screen, open the Settings app.
2. Navigate to the Wi-Fi menu.
3. Select the Wi-Fi network specic to the desired chemiSOLO unit. If a custom password was set
previously, input the password and select Join.
Note: The default settings for the system’s unique Wi-Fi system will be “chemiSOLO_[system serial
number]” with no password. For reference, each unit’s serial number can be located on the back of
the instrument.
4. Once the iPad is connected to the chemiSOLO’s Wi-Fi, open a web browser and input the
chemiSOLO’s Wi-Fi IP “192.168.12.1” to access the control software or alternatively, scan the QR
code on the inner lid of the unit to quickly access the same address.
2.5—External Wi-Fi Setup (optional)
Wi-Fi Dongle [CS1006] Setup
With the chemiSOLO Wi-Fi dongle accessory [CS1006] attached, the chemiSOLO can connect to an external
Wi-Fi network. This will allow devices connected to the chemiSOLO to simultaneously access both the
chemiSOLO control software and the internet via the connected network. Crucially, please keep in mind that
this setup will only work for the chemiSOLO Wi-Fi dongle accessory [CS1006], specically, and not for other
Wi-Fi dongles. To set up the external network, follow the steps below:
1. First, power on and connect to the chemiSOLO on an external device device (see section 2.4;
computer recommended).
2. In the chemiSOLO’s Settings menu, navigate to the Software tab.
3. Under External Network Connection, ll in the Network Name and Network Password of the external
network to connect the chemiSOLO’s Wi-Fi to.
4. Select Save, then restart the chemiSOLO with the Wi-Fi dongle accessory [CS1006] attached to
one of the USB ports on the back of the unit by turning the power switch off and back on. This will
implement the change and allow for internet access while connected to the chemiSOLO’s Wi-Fi.

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Direct Ethernet Setup
Enabling internet access while connected to the chemiSOLO’s Wi-Fi can also be accomplished through
direct ethernet connection with an active network modem. To set up the chemiSOLO this way, follow the
steps below:
1. Connect the chemiSOLO Ethernet Connector [CS1007] to the chemiSOLO Ethernet to USB Adapter
[CS1008].
2. Connect the ethernet side of the cord to the desired external internet modem.
3. Turn the chemiSOLO off, then connect the USB side of the cord to one of the two USB ports on the
back of the chemiSOLO unit.
4. Turn the chemiSOLO back on and the connection will be complete. With this connection setup, users
will have internet access while connected to the chemiSOLO’s Wi-Fi.