Azure Biosystems Cielo AIQ030 User manual

Azure Cielo™Real-Time PCR Systems
User Manual
Part Numbers AIQ030 and AIQ060

Azure Cielo Real-Time PCR Systems User Manual Page 2
Safety and Regulatory Compliance
Important Safety Information
Please read these instructions before operating the Azure Cielo system.
Electrical Safety Precautions
Be sure to take proper precautions when handling any electrical equipment. NEVER work on any live
circuit, xture, receptacle, or switch. Safety rules you should follow whenever working with any electrical
appliance include:
• Always shut off power at the main disconnect before changing a fuse.
• Always shut off power to the circuit before repairing or replacing a switch, receptacle, or xture.
• Always tape over the main switch, empty fuse socket, or circuit breaker on which you are working.
• Always check that the circuit is dead before beginning work. Using a circuit tester or voltmeter can
help determine this.
• Always unplug any appliance before attempting repair.
Protective ground terminal
The ground terminal is located on the inside of the back panel. It is intended to protect against
electric shock in case of a fault.
NOTE: This equipment has been tested and found to comply with the limits for a Class A digital device,
pursuant to Part 15 of the FCC Rules. These limits are designed to provide reasonable protection against
harmful interference when the equipment is operated in a commercial environment. This equipment
generates, uses, and can radiate radio frequency energy and, if not installed and used in accordance with the
instruction manual, may cause harmful interference to radio communications.
Operation of this equipment in a residential area is likely to cause harmful interference in which case the user
will be required to correct the interference at his own expense.
Changes or modications not expressly approved by the party responsible for compliance could void the
user’s authority to operate the equipment.
Hot surface warning
Under normal conditions, the temperature of plate is below 50°C and safe to touch. It is
possible that the glass surface temperature will exceed 80°C in rare instances. Please
exercise caution when contact with the plate surface is expected.
CAUTION: No user serviceable parts inside!
For Research Use Only
This instrument is suitable for research use only. It must be used, therefore, only by personnel who know the
health risks associated with the reagents that are normally used with this instrument.

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Warranty
The Azure Cielo products are warranted against defects in materials and workmanship for one year unless
otherwise outlined on your sales order or www.azurebiosystems.com/warranty.If any defect occurs in the
instrument during this warranty period, Azure Biosystems, Inc. will repair or replace the defective parts at its
discretion without charge. The following defects, however, are specically excluded:
• Defects caused by improper operation.
• Repair or modication done by anyone other than Azure Biosystems or the company’s
authorized agent.
• Use of spare parts supplied by anyone other than Azure Biosystems.
• Damage caused by accident or misuse.
• Damage caused by disaster.
• Corrosion caused by improper solvents or samples.
Voltage Setting Information
The Azure Cielo system has a power supply that automatically chooses the correct voltage for your country
or region.
CE Conformity
The following Cielo Model Systems, models: Cielo3 and Cielo6 are in conformity with the provisions of the
following EC Directives, including all amendments, and national legislation implementing these directives:
• EN61010-1: 2010
• EN61326-1: 2013
• EN61326-1:2013
• EN301489-1 v2.2.1:2019
• EN301489-17 v3.2.0:2017
• EN300238 v2.1.1:2016
Protection category: IP20 according to IEC 60529

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ENG
This symbol indicates that the waste of electrical and electronic equipment must not
be disposed as unsorted municipal waste and must be collected separately. Please
contact an authorized representative of the manufacturer for information concerning
the decommissioning of your equipment.
FRA
Ce symbole indique que les déchets relatifs à l’équipement électrique et électronique
ne doivent pas être jetés comme les ordures ménagères non-triées et doivent être
collectés séparément . Contactez un représentant agréé du fabricant pour obtenir des
informations sur la mise au rebut de votre équipement.
GER
Dieses Symbol kennzeichnet elektrische und elektronische Geräte, die nicht mit dem
gewöhnlichen, unsortierten Hausmüll entsorgt werden dürfen, sondern separat behandelt
werden müssen. Bitte nehmen Sie Kontakt mit einem autorisierten Beauftragten des
Herstellers auf, um Informationen hinsichtlich der Entsorgung Ihres Gerätes zu erhalten.
ITA
Questo simbolo indica che i riuti derivanti da apparecchiature elettriche ed elettroniche
non devono essere smaltiti come riuti municipali indifferenziati e devono invece essere
raccolti separatamente. Per informazioni relative alle modalità di smantellamento delle
apparecchiature fuori uso, contattare un rappresentante autorizzato del fabbricante.
SPA
Este símbolo indica que el equipo eléctrico y electrónico no debe tirarse con los
desechos domésticos y debe tratarse por separado. Contacte con el representante local
del fabricante para obtener más información sobre la forma de desechar el equipo.
SWE
Denna symbol anger att elektriska och elektroniska utrustningar inte får avyttras
som osorterat hushållsavfall och måste samlas in separat . Var god kontakta
en auktoriserad tillverkarrepresentant för information angående avyttring av
utrustningen.
Waste Electrical and Electronic Equipment (WEEE)
Contact
Azure Biosystems, Inc.
6747 Sierra Court, Suite A-B • Dublin, CA 94568 • USA
[e-mail] • [phone] • Fax [phone]

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Table of Contents
1. Introduction 6
1.1 Azure Cielo Specications 6
1.2 Plates and Tube Compatibility 7
1.3 Contacting Technical Support 9
2. Installation and Setup 10
2.1 System Placement 10
2.2 System Overview 10
2.3 Connect Azure Cielo Real-Time PCR system to Internet Network 11
2.2 Connect Azure Cielo Real-Time PCR system to PC via Azure Cielo
Manager software 11
3. Device Software Overview 14
3.1 The Home Screen 14
3.2 Experiment Setup 14
3.2.1 Protocol Manager 15
3.2.2 Change Protocol Properties 17
3.2.3 Well Selection Screen 19
3.2.4 Email Feature in Protocol Setting 19
3.2.5 Run Setup 20
3.2.6 Run Protocol 28
3.3 Data Manager 30
3.4 Device Settings 34
3.4.1 General 34
3.4.2 Network 35
3.4.3 Email Server Setup 35
3.4.4 Technical Support 37
4. Frequently Asked Questions 38

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1. Introduction
The Azure Cielo Real-Time PCR instrument is a high-performance tool compatible with a wide variety of
reagents for quantitative PCR. The family of instruments includes the Azure Cielo 3 and the Azure Cielo 6.
1.1—Azure Cielo Specications
The Azure Cielo instruments have the following specications.
Specications Azure Cielo 3
Real Time PCR System
Azure Cielo 6
Real Time PCR System
Part number AIQ030 AIQ060
Product description 96-well Real-Time PCR instrument
with 10.2” touchscreen interface,
3 dye channel lters
96-well Real-Time PCR instrument
with 10.2” touchscreen interface,
6 dye channel lters
Sample capacity (wells) 96 96
Reaction volume 5–150µL (10–50 µL recommended) 5–150µL (10–50 µL recommended)
Excitation source LED LED
Detection channels 3 6
Multiplexing Up to 3 targets Up to 6 targets
Thermal element Peltier Peltier
Max. block ramp rate 6°C/sec 6°C/sec
Avg. sample ramp rate 4°C/sec 4°C/sec
Temperature uniformity ±0.2°C ±0.2°C
Temperature accuracy ±0.1°C ±0.1°C
Dye compatibility SYBR Green, EvaGreen, FAM,
VIC, JOE, HEX, CAL Fluor 540,
CAL Fluor Orange 560, Cy5, LIZ,
Mustang Purple
SYBR Green, EvaGreen, FAM,
VIC, JOE, HEX, CAL Fluor 540,
CAL Fluor Orange 560, ROX,
TAMRA, TEX615, Quasar 670,
CAL Fluor Red 610, Cy5, LIZ, Mustang
Purple, Cy5.5, Quasar 705
Custom dye/chemistry üüüü
Chemistry capability Fast/Standard Fast/Standard
Detection sensitivity 1 copy 1 copy
Sensitivity Detect differences as small as 1.5-fold in
target quantities in single plex reactions
Detect differences as small as 1.5-fold in
target quantities in single plex reactions
Connectivity USB, Wi-Fi, Ethernet USB, Wi-Fi, Ethernet

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1.2—Plates and Tube Compatibility
Azure Cielo Real-Time PCR system is compatible with:
A. Low-prole 96-well Plates
• Low-prole plates have wells of approximate height 15.5 mm (depending on manufacturer).
These modern designs reduce condensate formation and allow for accurate light detection in
uorescence assays, low-volume reactions, and fast PCR.
• Non-skirted Plate: This plate lacks a surrounding panel. This format ts blocks of most thermal
cyclers and qPCR systems, but may not be suitable for robotic applications without stabilization.
• Semi-skirted Plate: This plate has a short panel around the edge of the plate, providing adequate
support during pipetting and mechanical strength for robotic handling.
*Azure Cielo Real-Time PCR Systems cannot accommodate a fully skirted 96-well plate.
Azure Cielo Systems are compatible with only a non-skirted or semi-skirted 96-well plate*
• Plate color can be White, Frosted or Clear. For assays requiring higher uorescent sensitivity and
accuracy, white or frosted plates would be recommended.
• Plates should be sealed with Optical Grade Seal Film. Detection of this system is from above and
through the top of each well in the plate. Signicant signal loss should be expected if not using
optically clear caps or seals.
• Plate rim (lip): The “rim” of a 96-well plate is a peripheral upper elevation around the plate.
Rimmed plates are NOT compatible with the Cielo system.
• Plate Notch: The notch is a modied corner of a 96-well plate. The Azure Cielo Systems are
versatile and can accommodate notching at any corner position. (A1,H1, A12 and H12)
*The Azure Cielo Systems are not compatible with any 384-well plate*

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B. Low-prole Tubes
• Tubes are an ideal choice for qPCR experiments of low to medium throughputs. Individual and
tube strips are two most common formats available. Tube strips are available in 8 or 12 well type.
Cielo systems are compatible with 0.1mL and 0.2mL tubes.
• Color of the tube(s) can be White, Frosted or Clear. For assays requiring higher uorescent
sensitivity and accuracy, white or frosted tubes would be recommended.
• The tubes should be sealed with Optical Grade Flat Caps or seals. Detection of this system is from
above and through the top of each tube. Signicant signal loss should be expected if not using
optically clear caps or seals.
• Recommended tube cap features should include a tight t in addition to optical clarity. Cap strips
are the most common way to seal tubes and plates. Azure Cielo Real-Time PCR systems are
compatible with at caps, that are optical clear or ultra-clear.
*The Azure Cielo Systems are not compatible with domed caps for tubes or plates*
C. Only a few samples
While the Cielo Real-time PCR system is designed for 96-well plates, many times researchers do not
need to run ninety-six samples. It is common to run only a few samples at a time, especially when
new assays or tests are being evaluated and optimized for best performance.
The lid and detection manifold are very well designed for even heating and detection over the 96-well
landscape. When only running a small number of samples, it is possible uneven pressure over these
few tubes due to the uneven settling of the lid manifold, might damage the strip or single tube
samples during the run.
The easy remedy to this is to evenly distribute samples or strip tubes to different sections of the
96-well plate area. Empty tubes can be used as placeholders at the edges of the 96-well plate area to
allow even pressure from the lid as the run progresses.
Figure 1. Orange circles represent samples to be run. Blue
circles represent empty tubes used as placeholders to ensure the
lid manifold applies even pressure over the entire 96-well area.

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1.3—Contacting Technical Support
For questions about installation, setup, or general use of the system, please contact
[e-mail] or call [phone], 9am–4pm Pacic time.
Figure 2. Filled orange circles show locations of samples across the 96-well format. There are a total of six
samples in tubes. Panel A shows an uneven distribution which may result in deformed or altered tubes at the
completion of the run. Panel B shows a more even and recommended distribution of the same six samples using
two blanks (blue lled circles).

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2.1—System Placement
WARNING: Excessive Weight Hazard –Please use two or more people to lift the system. Failure to do so
can result in system damage and personal injury.
As with all electrical instruments, the Azure Cielo should be located away from water, solvents, or corrosive
materials, on a at and stable surface with adequate clearance on all sides. The system must remain
stationary during operation.
The system should be placed away from interfering electrical signals and magnetic elds. If possible, a
dedicated electrical outlet should be used to eliminate electrical interference from other instrumentation in
your laboratory.
The Azure Cielo should be installed at no more than 3000 meters above sea level.
2.2—System Overview
2. Installation and Setup
Figure 4. Side view of the Azure Cielo Real-Time PCR systems.
Figure 3. Front view of the Azure Cielo Real-Time PCR systems.
Touch screen panel
USB Type A port
Door
Start button with indicator LED
Open button
Figure 5. Back view of the Azure Cielo Real-Time PCR systems.
Power Switch
Power Input USB Type A port
Ethernet port
USB Type B port