Baker SterilGARD II SG400STD User manual

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OPER~~TOR'S MANUAL
Baker SterilGARD ®II
Biological Safety Cabinet
MODELS
SG400STD, SG600STD ,
SG400ULT, SG600ULT
THE BAKER COMPANY
NSF classification: Class II, Type A. (When the unit is vented to the
outside, the classification is Class II, Type B3).
This manu al includes information for installation, op eration, maintenance
and sp are parts. We recommend that it be kept near the cabinet for ready
reference.
No v ember , 1998
Revised: 10-2009

THE BAKER COMPANY
INTRODUCTION AND WELCOME
It is a pleasure to welcome you to the growing number of customers who own and operate
Baker biological safety cabinets. As the inventors of the laminar flow biological safety cabinet
and the leaders in the field, Baker people take special pride in providing a cabinet which is
designed for maximum performance.
Your
new SterilGARD®ll has a number of remarkable features. Among them, the
contaminated positive pressure areas, which include the work area, are surrounded by a
negative pressure plenum. Tilis arrangement provides an extra measure of protection because
any particle in the contaminated zone will invariably be drawn through the blower and
trapped on a filter.
You
will find your SterilGARD®II cabinet suitable for use not only with l.V. drug
preparations and other pharmaceuticals, which could have adverse health effects on operators,
but also for clinical diagnostic work involving tissue culturing of possibly infectious samples
and other techniques requiring a contamination-free ahnosphere.
Please note that all open-front containment cabinets, including this one, are for use with low
to moderate risk agents only. Open-front cabinets do not provide absolute protection for the
user. The adequacy of a cabinet for user safety should be determined on-site by an industrial
hygienist, safety officer or other qualified person. Remember that you, the owner and user, are
ultimately responsible and that you use your cabinet at your own risk.
Built to exceed all microbiological aerosol tests specified by applicable NSF standards, your
SteriJGARD"'l! unit includes many unusual Baker features which are included for superior
performance, simpler maintenance and lower life cycle cost. Your SterilGARD8 II mut .is
designed for both safety and value.
We
recommend this manual, along with the factory test report that is included, be kept near
the cabinet for convenient reference by operators and qualified maintenance personnel. If you
have any questions about the use or care of your new SterilGARD"'ll cabinet, please do not
hesitate to contact our Customer Service Department for assistance.
SiHcerely,
Dennis Eagleson
President
The Baker Company, Inc.
P.O. Drawer E, Sanford, Muine 04073 (207) 324-87731-800-992-2537 FAX [phone]
"Creating immaculate atmo.rpheres"
1

TABLE OF CONTENTS
IN1RODUCTION ........................................................................................................ Page i
I
FUNCTION AT\JD DESCRIPTION OF THE STERILGA.t~D'II CABINET................... 1
Airflow Inside the Biological Safety Cabinet ................................................................. 1
Positive and Negative Pressure Areas ........................................................................... 2
Access to the
Work Area ................................................................................................. 2
Design Details ................................................................................................................... 2
S
"f" . .1
peG 1cahons .................................................................................................................... ~
II PREPARING THE STERILGARD®II CABINET FOR USE ..... ... .. ....... ... .................... 5
Checking the Cabinet on Arrival ..................................................................................... 5
The Uses of a Biological Safety Cabinet ......................................................................... 5
Cautions ............................................................................................................................ 5
Location Within the Laboratory ...................................................................................... 5
Installing the Cabinet ....................................................................................................... 6
Assembling the Footrest .................................................................................................. 7
Connecting the Exhaust ................................................................................................... 9
Final Connections and Tests ............................................................................................ 9
III PROPER CABINET USE ................................................................................................ 11
Operator Controls .......................................................................................................... 11
Air Pressure Monitor /Magnehelic Gauge ................................................................... 12
Ground Fault Interrupter .............................................................................................. 13
Start-up Procedure ......................................................................................................... 13
Working in the Cabinet Work Space ............................................................................ 13
Reacting to Spills ............................................................................................................ 14
Ultraviolet (Germicidal) Light ...................................................................................... 15
Decontamination ............................................................................................................ 15
Using Ancillary Equipment .......................................................................................... 17
About the HEPA Filters ................................................................................................. 17
Operating Procedures .................................................................................................... 18
IV ON-SITE CHECKS AND MAIN"TENAl\JCE ............................................................... 19
The Airflow Balance ...................................................................................................... 19
Filter Media and Seal Leak Tests .................................................................................. 20
Airflow Smoke Pattern Test .......................................................................................... 20
Cabinet Integrity Test .................................................................................................... 20
G , d. r f ··t ~- ·t "1
roun mg ~on rnm y l es . .. . .. ........... .. . .. .... .. ... .. .......... .. . .... .... .. ... .. ........... .. . .. ..... .. .... .. . ~
fv1aintenance ?-.Jotes ......................................................................................................... ::?.1
Replacing the HEP A Fil ten; ........................................................................................... 21
Trc,1Jbleshc)oti11g ............................................................................................................. 23
ll

APPENDIX ................................................................................................................... Page 26
Wiring Diagram, SG400 Models ................................................................................... 26
Wiring Diagram, SG600 Models.................................................................................... 27
Dimensional
Drawing, Model SG400STD ................................................................... 28
Dimensional
Drawing, Model SG600STD .............................................;...................... 29
Dimensional Drawing, Model SG400ULT.................................................................... 30
Dimensional Drawing, Model SG600ULT ................................................................... 31
Replacement Parts List, Models SG400STD & SG600STD .......................................... 32
Replacement Parts List, Models SG400ULT & SG600ULT........................................... 33
Warranty .......................................................................................................................... 34
LIST OF FIGURES
Fig. 1 Airflow Inside Cabinet .............................................................................................. 1
Fig. 2
Adjusting the Telescoping Leg Assembly ................................................................ 6
Fig. 3 Installation
Without Lifting Equipment ................................................................... 6
Fig. 4
Assembling the Footrest ............................................................................................ 8
Fig. 5
Operator Controls ..................................................................................................... 11
111

I -FUNCTION AND DESCRIPTION OF THE STERILGARD®II
CABINET
The SterilGARD"'II is a biological safety cabinet
of original design. It features vertical laminar
airflow and a front access opening. The ru1it is
designed to protect not only the environment and
the people using the cabinet, but also the product
within from airborne contaminants.
Airflow Inside the Biological Safety Cabinet
The SterilGARD® II cabinet features The Baker
Company's zoned airflow principle. See Fig. 1.
The stainless steel metal diffuser just below the
supply filter creates a faster airflow at the front of
the work zone than at the center. The protective
faster airflow in front makes an extremely
effective air barrier.
A feature of the unique Baker design is the high
volume return air slots, which maximize the
cabinet's protective capability. It is generally
accepted that maintaining containment and a
particle free work area is most difficult in the area
in whim airflow turbulence is greatest- at the
intersection of the side walls, the front access
opening and the work surface. Turbulence
caused by friction will also be found along a
cabinet's side walls. ln cabinets without high-
velocity return air slots, this turbulence may allow
contaminants to escape from the work area, or it
may make it possible for unfiltered room air to
enter the work area.
The Baker high-velocity retu.m air slots are
located along the side walls of the work area. Air
is dravv:n into the slots at very high speed,
preventing the escape of particulates and
ensuring that no unfiltered air enters the work
area. Additional hjgh-velocity return air slots are
located at the top and sides of the viewscreen to
prevent gases, \'·apors or particulates from con1ing
up behlnd the vie\vscreen and escaping into the
laboratory. In the same \va.L they' prevent room
air .frorn do>A'T1 behJnd tl1e \/ie'i\rscreen
and contarninatLTlg the work area.
1
P!enum
~
~
<}
t
;;J
-
Blower
v v v
v v v
t::J;;J
-
Room air
negative pressure
+ Contaminated air
positive pressure
<> Clean air
.qp. Contamina~ed air
negative pressure
Exhaust
)I> filter
Supply
filter
~
8" access
----
opening
Fig. 1 Airflow Inside Cabinet

Positive and Negative Pressure ~<\reas
One of the n1any features that sets the
SterilGARD"':-n
unit apart frorn oL~er cabinets is
the interaction of positive cu1cl negative pressure
areas. The srrtalJ posibve pressure pienwn is
cornpleteJy surrollnded by a negati'i/e pressure
area so that any partide passing through is
invariably drawn thrm1gh the blower and trapped
in a filter.
From the small positive pressure plenum, which
is shown .in Figure 1, about 30 percent of Ll-te total
cabinet airflow is expelled through the exhaust
filter. The remaining 70 percent is recirculated
through the supply filter and re-enters the work
area as particle-free air. Because the cabinet must
take in air to replace the air expelled through the
exhaust filter, the same volume of room air enters
the cabinet through the 8-inch front access
opening. This air, which never enters the work
area, cornp letes the air bar.rier at the front access
opening.
All air is combined under the work surface.
From here, the contaminated air is pulled, under
negative pressure, through a plenurn up the rear
of the cabinet into the motor-blower, which blows
it into a positive pressure plenum. Please note
again that the positive pressure plenum is
completely surrounded by negative pressure
areas. If a leak should occur in a contaminated
negative pressure plenum, or in the side wall or
rear wall, the negative pressure will create suction
and pu !I air in, not allowing it to escape into the
operator's area. If there is a leak from the positive
pressure plenum, the surrounding negative
pressure area will recapture the contamicnated air
and recirculate it through a filter.
Access to the Work Area
For easy entry of apparatus into the work area,
SterilGARD"'ll has a vertical sliding viewscreen .
..
Its \4-inch safety plate glass allows excellent
visibility and may be opened to a height of 18 W'
to permit placement of items in the work area.
TI1e window should be set at a height of 8" while
work is being performed, and an alarm is
provided to remind the operator if the window is
not in the correct position for using th.e cabinet.
(Chapter 3 on "Proper Cabinet Use'' will explain
ho\v to use the ·v-ieTNscreen correctly vvlli]e the
cabinet is in use.)
As with other Baker cabinets, the electricctl
outlets Lnside the \Vork. area have a circuit bre::tker
so that an electrical overload by ancillary
equipment will not affect air handling.
Overloading with electrical equipment should, of
course, be avoid.ed in any case. (See th.e
"A.ncillary
Equipment" instructions in Chapter 3
on "Proper Cabinet Use", and in the "Appendix.")
Design Details
Performance assurance
Meticulous care in manufacturing is followed by
more than 14 separate performance tests prior to
shipment of your cabinet. In addition, a complete
factory test report on the performance of your
unit is included at the back of this manual.
Motor/ blower capacity
A motor/blower's capacity is measured by its
ability
to provide a nearly constant volume of air
as resistance increases becau.se of filter loading.
Verification by a simulated filter loading test has
established that your SteriiCARD"Il cabinet is
capable of auto~atically handling an 80% (in both
4-foot and 6-foot 1mit) increase in pressure drop
across the filter without reducing total air delivery
more than 10~/o. A convenient magnehelic gauge
(or bar graph display on the ULTRA models)
shows the current relative performance of the
blower. With the use of the manual speed control,
a 165');,/180% (4-foot/6-foot unit) increase in the
pressure drop across the filter can be handled.
The resulting long filter life means significant cost
savings.
One-piece interior wall construction
The interior side and rear walls of your
Steri!GARD®Il's work area are constructed from a
single piece
of 16-gauge stainless steel. The easy-
to-clean 7 /16-inch radiused (rounded) comers
prevent buildup of contaminants and resist
corrosion.
Protective screen
Located under the work surface at the bottom
and sides of the rear return-air plenum, a

protective screen is provided to help prevent
wipes and other paper materials from being
drawn into the blower system. This precaution
eliminates costly decontamination and downtime.
The screen affords easy access to waste materials
and should be kept clean at all times.
All-metal plenums
The plenums of your SterilGARD"II unit have
been constructed entirely of metal in order to
provide strength, durability, air-tightness and
resistance to deterioration.
Recessed stainless steel work surface
The work surface is constructed of corrosion-
resistant 16-gauge type 304 stainless steel. Its
satin finish diminishes light reflection. It is
recessed to retain spills, and the 3/16" radiused
con1ers make for easy cleaning.
Cabinet exterior
Extemal construction is of 14-gauge cold-rolled
steel, protected by a smooth white baked enamel
finish.
Tested HEPA!ULPA filters
Supply and exhaust filters in SterilGARDeii are
zero-probed HEPA. They have been found to be
99.99% effective on particles of 0.3 micron size. In
the SterilGARD''II ULTRA 4-foot model only,
supply and exhaust ULPA filters are used, whicll
are 99.999'Yo effective on particulates of 0.12
micron. Eacll filter is scan-tested to assure leak-
free installation.
Viewscreen
The cabinet's %-incll safety plate glass vertical
sliding viewscreen may be opened to 18 Yz" for
placement of large items in the work area, and
may be fully closed for system shutdown.
Drain valve
MeetL"1g NSf requirements for drainage in Class
II biologicaJ safety cabh"'"tets, a stainless stee] baJJ
>.raJve i_n the SterJJGARD~]l unit is installed for
sc:Je effective d.rainage of the drain pan.
Work area lighting
SterilGARD"'II's lighting system produces 100 to
150 foot candles of illumination at the work
surface area. The fluorescent tight is extemall.y
m01mted to minimize heat buildup. The unit uses
two common fluorescent bulbs.
Air balance adjustments
Air balancing can be done by either of the
following methods. It should, however, be done
only by a teclmician with proper training and
equipment. (See Section 4, "On-Site Checks and
Maintenance Procedures.")
• A
speed controller adjusts for voltage
differences and filter loading.
•
An adjustable damper compensates for
differences
of supply and exhaust filters if
they are changed at a later date.
Easy filter access
For convenience
and ease of service, the supply
and exhaust filters are loaded and removed from
the front of the cabinet without entry into
SterilGARD'''Il's work area. This is particularly
helpful if the cabinet is connected to an exhaust
duct. Filters should be replaced by qualified
technicians on!
y.
Stainless steel telescoping leg assembly
TI1e Steri!GARD"II unit has a distinctive
assembly with four telescoping legs, whicll allows
the cabinet to be shipped with a minimum height
of 74 7/8". The assembly also makes it possible to
adjust the cabinet to two heights, providing a
work surface of either 30" or 36" above the floor.
Leg I Knee
Work
Exten- Space Surface
Overall
sion I Height
Height
Height
Shipping &
11~~./-,;
" I < 74
cIs"
--I
fnstallation I I
Setting A i
25'"
) 26''* I
30'''!"
I
891/o" i
'
'
~
/ Settmg B
'I ., ' 0" •• 1
.. ) ""! • ! /~:;, :/ b !
levelerc;

Specifications
Weight
The weight of the SterilGARD'"II 4-foot cabinet
is
630 pounds with a shippirtg weight of 875 lbs.
A 6-foot cabinet weighs
840 pounds with a
shipping weight of 1075 pounds.
Electrical specifications
115V AC, 1 Phase, 60 Hz
The SterilGARD0 11 incorporates a solid-state
controller for
the blower motor. This compensates
for variations
in incoming line voltages. The
usable voltage range is 95 to 130V AC
The unit is provided with two duplex G.FJ.
receptacles
at a total of 5.0 amps, controlled by an
internal self-resetting circuit breaker. The unit is
furnished with one 12' power cord with 20-amp
plug, type NEMA 5-20P.
Blower motor (1 ,625 RPM)
Fluorescent Light (ballast)
Duplex outlets (G.F.!.)
Total
running load
SG400/SG600
6.6 amps/9.9 amps
0.7 amps/0.7 amps
5.0 amps/5.0 amps
12.3 arnps/15.6 amps
4

II - PREPARING THE STERILGARD®II CABINET FOR USE
Checking the Cabinet On Arrival
Upon receipt of your new SteriiGARDoll
cabinet, first inspect the exterior of the crate and
skid. If there is any broken glass or other visible
damage, that fact should be noted on the
receiving slip and immediately reported to the
delivering carrier.
Now remove the crate and inspect the tmit
itself. The top cover of the crate should be taken
off first, then the boards from front and back.
Bend both ends of the crate outward away from
the sides of the unit and remove front or rear
blocking from the skid. Remove the cabinet from
the skid with a fork lift or other available
equipment. If any concealed damage is found it
should be reported to the delivering carrier, who
will want m1 opportunity to inspect the damage.
A claim for restihttion shollld be filed within 15
days.
. Because of the dwger of mishandling by
trucking companies, we have removed certain
parts of the cabinet and have packed them
separatelv. These items are listed on the packing
slip which accompanies the unit. Please check the
packing slip carefully to be sure that all items
have been located.
The Uses of a Biological Safety Cabinet
The SterilGARD"II cabinet has been designed to
provide a work area which protects the
experiment from the environment, and the
enviroruncnt frorn the experi-ment. The larninnr
flow biological safety cabinet is designed for work
with Biosafety Levels 1, 2 and 3 (low to moderate
risk) agents as listed in The Center for Disease
Control's ~~Biosafety in lviicTobiological and
Biomedical Laboratories", U.S. Department of
Health and Hllman Services, Public I-Iealth
Service, Centers for Disease Control and National
Institutes of Health, L.S. Govenunent Printing
Office, Washington, D.C. 20402. HHS publication
nurnber (CDC! 34.-3395.
Biosafety level 4 or extremely f'Ligh risk agents
sl1ould neve·r be used in ul-lls cabinet. Please
5
consult your safety professional for a proper risk
assessment.
C4.UTIONS
* T1w use of any hazardous material in the
cabinet requires
that it be monitored by an
industrial hygienist, safety officer or other
qualified i>u/ividual.
* Explosive or flammable su/Jstances should
never be used in the cabinet unless a quali-
fied
safety professional has evaluated the
risk.
* lf hazardous biological work is to be per-
formed, apply the app1·opriate biohazard de-
cal which is enclosed. This is in accord
with
OSHA regulations, volume 39, 11umber 125,
PadiL
* If chentical, 1·adialogical or other n.on.micro-
bialogical hazards are present, be sure to
employ appropriate protective measures in
addition to formaldehyde decontamination.
Call upol! a suitably trailred individual to
monitor the operation.
Location Within the Laboratory
The ideal location for any laminar flow
biological safety cabinet is
in a dead-end comer of
the laboratory away from personnel traffic, vents,
doors, windows and any other sources of
dismptive air currents. Published research from
The Baker Companv (Rake, B.W. "Influence of
Crossdrafts" on the Perfonnance of a Biological
Safety Cabinet. Appl. Microbiology 36:278-283,
1978.) and w1published tests performed at the
National Cancer Institute show that i.f a draft or
other disruptive air current were to exceed the
intake velocity of the cabinet, then contarrlination
can e-nter the vvork area or escape frorn it. Proper
placement within the is essential.
If the cabinet exhausts Lts air into the Iaborrltonr
ins:tead of venting to the OL.ltsidef it is imoortant
that there be adequate space between th~ top of
t._he cabinet and the ceiling. A soJjd ceiling located

too close to the exllaust filter vvill restrJct the air
and limit the intake velocity.. t., mi_ni.mum of 12"
of clearance on all sides and top of the cabinet is
necessary to achieve ideal conditions for testing.
If a free space of 12" above the exhaust filter is not
available, inaccuracies in exhaust flow
measurements mav result. Consult with our
Customer Service Department for the implications
of this, and for alternatives.
Installing the Cabinet
Installation of this cabinet should be carried out
in accordance with appropriate OSHA regulations
and those of other regulatory agencies having
jurisdiction.
1. First, move the cabinet from the unloading
area to its intended location on dollies. The
overall dimensions in the shipping position
for the ·!-foot unit are: 34 :I 12" deep x 53 3I 16"
wide x 74 7 Is" high. For the 6-foot unit, the
overall shipping dimensions are: 34 1 /32"
deep x 77 '/1s" wide x 74 7 Is" high.
Glass window
) / (tron1 of oabine1)
Work surface opening
lower edge
Fig. 2 Adjusting the Telescoping Leg Assembly
6
~
0 J.l
\
CJ
0
vlegbolt
\\
-'-
,,
8" lift
•
Fig. 3 Installation Without Lifting Equipment
2. For convenience in moving the cabinet, its
depth may be reduced to 31 7Is" by removing
the light canopy assembly. First, with the
unit disconnected electrically, remove the two
nuts holding the canopy clips at the top of the
canopy. Then let the canopy pivot down
until the end restraints stop it. Disconnect
the electrical plugs inside on each end.
Disconnect the restraints and lift the canopy
up and off. 111en move the canopy to a new
location.
3. The unit can be assembled with the work
surface either 30" or 36" from the floor
according to the needs of the owner. The unit
is shipped with the legs extending 6" below
t.lte cabirtet. If lifting eqvipm-~nt js available_.
lift the cabinet vertically to a height of
approximately 40." All four legs may then be
extended to the desired height by removing
the four shipping bolts, setting the height of
the legs and then securing the legs in position
with the four bolts which are supplied with
the unit e Is" X 2 3I 4" -see Figure 2). use a
9
/16" wrench. TI1e cabinet must be held safely
at the •±0" height duri.rtg the entire operation,
and set back onto the floor only after all four
legs have been secured. Final leveling may

be accomplished with the adjusting leveling
pads, located at the bottom of each leg.
4. If lifting equipment is not available, the legs
rna y be extended to the desired height in the
following manner. It is recommended that at
least four people be used for this operation.
.
WA.RNING!
The 4-foot SterilGARD@II weighs 630 lbs.
(6-foot unit weighs 840 lbs.). ilt least four
people should be used to perf01~1t the
following pl'ocedure. .
Lift either the right or left end of the cabinet
approximately 8" to 9." See Fig. 3. Remove
the shipping screws and lower the free legs to
the next hole position in tl1e leg. Then
replace the shipping screws to the lock-in
position.
Repeat the step above by lifting from the
opposite end. This will bring the cabinet to a
level position. Extreme care should be taken
in this step to make sure the cabinet does not
slip on the floor.
Now repeat the steps above once to obtain
the sitting position height and twice to obtain
the standing position height.
:o. After the unit has been adjusted to the
desired position, insert the solid dot plugs
(shipped in a small bag on the work surface),
into the unused holes in the legs to prevent
dust and dirt from entering the legs through
the open holes.
6. The drain pan is located under the work
surface. During shipment, this work surface
is held in place by hold-down clips. Lift the
vvork surface and rernuve the hold-do\vn
clips.
7. Level the work surface by adjusting the
glides on ea.ch of the four con.1ers of the ieg
risers. Be sure that all four are finnly on the
tloor .so that the ce1binet does not teeter. ~..\.n
adjustrnent range of 1 1/ .{" i.s provided.
Adjust to obtain a work surface height of 30"
to 36."
j
8. Check to be sure that the liquid drain valve is
in the closed position. (The handle on the
valve should be parallel to the floor). If
something is spilled in the work area, it will
remain in the drain·system and wo11't reach
the laboratory floor. Precautions may be
required for the safe disposal of the effluent
from the drain .
9. Also check to see that service petcocks, if
present, are in the closed position. (The
handle on eilch valve should be
perpendicular to the rear wall of the unit if
installed
in the sidewalls).
10. Remove the hardboard (masonite) shipping
cover from the top of the ex.l-taust filter.
11. Remove the viewscreen shipping clips and
supports and carefully check the viewscreen
for smooth vertical operation. If the
viewscreen binds, adjust the track by
loosening the scre\1\'S, arranging the track in
the proper position, and then re-tightening to
hold the track in the correct position. In
order to be effective, the wiper gasket must
exert a modest amotmt of "drag" on the glass.
If the view screen is loosened, make sure that
the wiper still makes contact. Also remove
the shipping screws and clips from the front
and rear perforated grills.
Assembling the Footrest
1. Slide the right-hand and left-hand footrest
tube brackets over the front adjustable legs as
shown in Fig. 4.
2. Using the supplied hardware, secure each
h1be bracket to the nlounting hole. Each hole
is located 1H
3
Is" from the floor. Insert the
bolt from the rear, through the slot, and
through the hole of the adjusting leg. Be sure
that the slot on the underside of the bolt fits
into the slot on the bracket. This will prevent
the bolt fron1 turning as the assernb.ly is:
tightened. Slide the bracket 1.1p or dovvn so
the bolt is centered Lrt the \/erticZll slot. finJ::.h
securing each bracket using a 3 Is" washer, a
'• lockvvasher! and a iocking ha_ndle_
The handles may be adjusted to manv
positions. Pulling out on the handle

disengages it while the threaded portion
remains stationary. Releasing the handle re-
engages it for tightening and loosening. The
handles for the tube brackets should be set in
tlce vertical positions.
3. Find a helper to assist you. Slide the footrest
between the legs of the unit. On each side,
place the upper part (3 /s" dia. hole) over the
upper stud. The curving slot should fit over
the lower slot. On each side, secure the stud
at the top with the supplied hardware (3/s"
washer on inside, 3/s" lockwasher, and nut
on outside).
L. H. footrest
tube bracket
Footrest
bolt
Fig. 4 Assembling the Footrest
8
Set the footrest so the lower stud is centered
in the radiused slot. Secure the lower stud
with the supplied hardware e /s" washer on
inside, 3/
s" lockwasher, and nut on outside).
4. The height of the footrest may be adjusted
over the range allowed by the slots on the
tube brackets. The angle of the footrest may
be adjusted over a range of 20°.
Upper stud:
3/s" lockwasher
3/s" washer
3
/e'' nut
Lower stud:
3/s" washer
3fa" lockwasher
locking handle
R. H. footrest
tube
bracket
·_./r~f
~ ~'>,..
/1:- :::-mw- i
/ .:.-::....., 'i1
1
_.... '-!.- aj
/ '
~;.--
./
"
Footrest bolt
3fs" flat washer
3/a"
lockwasher
locking handle

Connecting the Exhaust
The SterilGARD@II cabinet can operate
with filtered exhaust air enteri11g directly
i11to the room, or with filtered exhaust
duded to the outdoors. Details of these
alternatives are as follows:
Exhaust into the room -
If the cabinet exhausts its air directly into the
laboratory instead of ducting it to the outside,
it is important that there be adequate space
between the top of the cabinet and the ceiling.
If the solid ceiling is located less than 4" from
the exhaust filter, it may restrict the air and
limit the cabinet's intake velocity.
Although 4'" of top clearance may be
sufficient for day-to-day operation of the
cabinet, it will be difficult to obtain airflow
testing information. At least 12" of clearance
on the top of the cabinet is recommended for
reliable rneasure.rnent of ai.rflovv.
Never use ttle top of the cabinet or the work
area for storage purposes, and never use
flac'Timable, explosive or toxic vapors or
gases, or substances which generate them,
unless a qualified safety professional has
evaluated the risk The filter removes only
particulates and not gases, thus causing the
recirculation of these within the cabinet.
Attach the long-legged exhaust filter guard,
located in the rear, to prevent inadvertent
blocking of the filter area.
Remove the two nuts from the back of the
exhaust filter guard, and then remove nvo
nuts from the front and two from the rear of
the exhaust filter housirtg on top of the unit.
Install the legs on the studs at the rear of the
exhaust filter housing. Tighten them dovvn
with the previously-removed nuts.
Install the exhaust filter guard, setting the
front clips on the front studs of the exhaust
filter housing. Tighten then1 down vvith
previously' rernoved nllts.
I'-Jov~_.· secure the filter g-uard to the ='is-inch
NPT nipples in the top.
9
Exhaust to the outside -
Whenever possible, the filtered exhaust
should be cormected to its own separate
exhaust svstem. If it m11st be channeled into a
multi-du~t system, make sure that the'system
is not a recirculating one. You will, of course,
also want to make sure that the system can
handle the volume of air required to pass
through it, and that there is sufficient static
pressure for proper cabinet function.
The exhaust requirements of the 4-foot
SterilGARD'fl are 268 CFM at .02"-.04" water
column suction directly above the exhaust
filter before any reductions, elbows or other
restrictions. If using Baker's hard exhaust
transition (ET400), add 0.10" water column.
If a Baker thimble exhaust transition is being
used, 322 CFM minimum at 0.10" water
column.
The exhaust reguirernents of the 6·-foot
SteriiGARD-!1 are 408 CHv! at .02"-.04" water
column suction directly above the exhaust
filter before any reductions, elbows or other
restrictions. If using Baker's hard exhaust
transition (ET600), add 0.10" water column.
If a Baker thimble exhaust transition is being
used, 462 CFM minimum at ()_22" water
column.
You may want to install an indicator light or
some other safety device to give warning if
the exhaust system fails. A properly
designed duct system includes an airtight
damper to balance the air and also to shut off
the duct for purposes of cabinet
decontamination. The Baker Cornpanv offers
a transition
duct piece for connecting the unit
to the exhaLLSt system. Call for details.
For
further infonnation, refer to the National
Sanitation Foundation's Standard J"Jo. 49 and
other guidelines regarding ·ventilation.
Final Connections and Tests
1. The pl'untbi_ng connection to the service
petcocks. rrrust be made with great care
because the effluent from a safety cabinet
rr1ay be biologically hazardous. VVhen
present, petcocks are piped ·vvithin the

cabinet. The external connection uses 3/8-
inch NPT nipples at the base of the cab~cet.
Connection to plant utilities should be made
with proper materials and technique. No
flammable gas should be used.• However, ii
the risk is professionally evaluated and a
decision is made to install a flammable gas
petcock, then an emergency shut-off valve
should be situated in an accessible location
outside the cabinet.
2. A 20-amp power cord with a NEMA 5-20P
plug is furnished with the SterilGARD"'II. It
should be plugged into an appropriate 115
Volt, 60 Hz, 20 amp dedicated utility outlet.
3. Press the blower switch. The yellow indicator
above the switch should light.
4. Before using the cabinet, tum on the
fluorescent light cmd make sure the bulb is
lighted. These bulbs are locked into place
with stop-lock fittings.
5. Allow the cabinet to run for about half an
hour so the dirty air in the work area will be
purged.
Leave the blower running and wash the
entire cabinet, inside and out, with a
detergent-disinfectcmt to remove surface
dust. Once started, we recommend that all
cabinets be left rurming continuously.
For additional start-up and use procedures,
please turn to Section 3, "Proper Cabinet
Use."
6. The SterilGARD®ll cabinet has been subjected
to a comprehensive series of physical tests
before shipment from the factory. A physical
test report is filed by serial number as a
permanent record at Baker headquarters, and
a copy of the report accompanies each
shipment. Your copy is at the back of this
manual.
10
7. Although all units are carefully tested at the
factory, it is advisable that certain other
checks be made on-site by a qualified
technician after installation. These include
testing the filters for leaks and checking the
air balance of the unit, especially ii it is
connected to an exhaust system. A
description of these tests can be found in
Section 4, "On-site Checks and Maintenance."
8. It is also recommended that all personnel
who will be using the cabinet study this
Operator's Manual to learn how to make the
most effective use of the unit. For additional
and start up and use procedures, please turn
to Section 3, "Proper Cabinet Use."
FOR IviORE INFORMATION
For a complete listing of articles, papers and
reports related to containment, clean ail·
products
and safety, contact The Baker
Company
for our complete Bibliography or
visit our website at www.bakerco.com.

m- PROPER CABINET USE
A biological safety cabinet is a valuable
supplement to good sterile technique, but is not a
replacement for it. If the cabinet is not
understood and operated correctly, it will not
provide an adequate protective barrier.
All activities that are to be performed in your
cabinet should first be approved by a competent
professional, such as an industrial hygienist or
safety officer, to make sure that the cabinet is
appropriate for the work it wiJl be required to do.
This person should monitor the cabinet and its
operating personnel at regular intervals to see that
it is being used correctly.
ln
order to keep the interior workspace clean
and free of particulates, all Baker laminar flow
cabinets are designed for continuous operation. If
the blower is turned off, the unit becomes
contaminated with room air. Therefore it is
recommended that the blower be left on.
2 · Slower On/Off
(blower on indicator· yellow)
Fig. 5 Operator Controls
1 - Alarm ReseV
Sash
Level Alarm
Air pressure monitor '\.
bar graph (mag gauge} , '\.
11
Operator Controls
The operator controls and indicators are
arranged on the front panel of the unit. See Fig. 5.
A number of switches are arranged in a single
membrane switch assembly.
1
-Alarm Reset Switch/ Sash Level Alarm -
For normal operation, the viewscreen must be
placed to allow an opening of exactly 8." This
setting is important to allow the correct airflow
into the work area. The window may also be
closed completely. If the window is left in any
other position, an alarm will sound. An indicator
above
the alarm reset switch will flash.
To
mute the audible alarm, press the alarm reset
button. The indicator will continue to flash. After
five minutes, the alarm will sound again to
remind you to lower the window. You may press
the alarm reset switch again to mute the alarm for
another five minutes. The alarm will sound again
if the view screen is not in the p1·oper 8" position.
3 -Outlets On/Off
(oUllets on indicator- yellow)
4- Light On/Off
(light
on indicator- yellow)
S- UV Light On/Ott
(UV light on indicator -yellow)
~ Light canopy

When the unit is operating, the viewscreen
must be open exactly 8", or must be completely
closed. At any other setting, the sash level a lam1
indicator will flash.
2- Blower On/Off Switch-
This switch controls the power to the blower for
the unit.
When the switch is turned on, the
yellow indicator will light. The lights and outlets
will not operate unless the blower switch is
turned on first.
The indicator light above the switch shows that
the switch has been turned on.
3- Outlet On/Off-
This switch controls the two outlets on each side
of the work area. When the outlets are turned on,
the yellow indicator above the switch will light.
The indicator above the switch will light when
the outlets are turned on.
4- Light On/Off Switch-
This switch controls the fluorescent light inside
the work area. The blower must be turned on
first. The fluorescent light will not operate if the
UV light is already htrr ed on.
The indicator above the switch will light when
the fluorescent light is turned on.
5- Ultraviolet (Germicidal) Light On/Off Switch
A bulb which produces light in the ultraviolet
range (UV) may be used to help disinfect the
work area. This switch controls the UV light
inside the work area. Before the UV light may be
turrted on, the blower n1ust be turned on, the
viewscreen must be fully closed, and the
t1uorescent light must be turned off. The UV light
will shut off if the view screen is opened.
The indicator above the switch will light when
the UV light is turned on.
Air Pressure Monitor
The air pressure monitor is mounted on the left
side
of the control panel (comes standard on SGII
ULTRA model and as an option on the SGII
3tandard model). See Fig. 5. The bar graph
12
shows the relative cabinet perfonnance, measured
in terms of the total volume of air being handled
bv the unit.
The top
of the lighted area indicates the relative
airflow. When the airtlow is normal, the LEUs in
the center of the bar graph will glow with a steady
green light.
If just one or two LED's at the bottom of the
graph glow with a red light, the milt is handling a
low volume of air. An alarm will also sound.
This indication mea11s that the unit is not
receiving enough intake air.
.··
vV..4RNING!
If the unit continues to operate in this
condition, it is possible that contamination
may spread outside of the work area. The
product inside the work area may also be
cmttil1n.ina.ted. If you see the- lo1.V airflow
indicator, take these steps:
• Lea<?e the unit running. The. blower will
continue to move air through the cabinet
and filters.
• Close the window completely.
• Call a supervisor and! or a servtce
· ·. techttician.
lf the two LED's at the top blink with a green
light, this indicates a high air flow condition. If
the sash is lowered and the UV light is on, this is a
safe condition.
However, ii you are working in
the cabinet or the sash is at the operating height
(8"), check the intake grilles for blockage. If this
condition persists, call a supervisor or main-
tenance technician. Product protection may be
compromised.
Magnehelic Gauge
Your SteriJCARD"'JI mav be equipped with a
magnehelic gauge in place of the air pressure
monitor (all SCI! Standard models feature the
rnagnehelic gauge). The magnehelic gauge
should be zeroed after the unit has been leveled,
and before it is injtiallv turned on.

The purpose of the magnehelic gauge is to
measure pressure in the filter plenum. Tnis gauge
cannot be used to measure cabinet airflows. It
gives an indication of static pressure in the filter
area. If the reading is lower that normal, it may
suggest improper blower operation. As filters
load, the gauge indication will rise slowly to show
a higher pressure required to deliver the same air
volume through the filters. Tius is not, however,
a direct reflection of airflow.
Ground Fault Interrupter
The outlets on this unit are protected by a
ground fault interrupter (GEL). The G.FL is
designed to protect the operator from a possible
electrical hazard. If the G.F.L detects a hazardous
condition, it will cut off electricity to all of the
outlets. The button in the center of the unit will
pop out To reset the G.F.L, correct the cause of
the problem, then press the button in.
Start-up Procedure
1
1. If the tmit has not been left running
continuously, press the blower on/ off switch.
The yellow indicator above the switd1 will
light Make sure that you have cabinet
airflow, either by listening for blower sound
or feeling the airflow across your fingers.
Check the reading on the air pressure
monitor (magnehelic gauge). See Fig. 5. The
reading on the monitor (magnehelic gauge)
should be consistent with the last time the
unit was on.
2 Turn on thf'> fluoncscent J;ght. The fluorescent
light will not operate unless the ultraviolet
J;ght is turned off. Never leave the
ultraviolet light 011 while there is anyone ilz
the roont.
3. Check to determine that the drain v'alve is in
the closed position or the drain coupling is
capped.
4. \Alipe do·vvn the interior area of the cabinet
vvith a surface disinfectant.
Note:
Some disinfectants may corrode or
stain the steel surfaces. If this happens, clean
13
the surfaces afterward with a detergent and
rinse with sterile water to prevent corrosion.
5. Place all materials to be used for the next
procedure inside the cabinet. Disinfect the
exterior of these materials.
Everything required (and nothing more)
sbould be placed in the cabinet before
beginning your work so that nothing passes
in or out through the air barrier until the
procedure is completed. Implements should
be arranged in the cabinet's work area in
logical
order so that cle~n and dirty materials
are segregated, preferably on opposite sides
of the work area.
Blocking
tl1e front and rear perforated grills
must be avoided. If wipes or absorbent
towels are used on the work surface, be sure
to keep them away from the grills.
6. After your equipment is in place inside the
cabinet, adjust the vertical sliding viewscreen
so ii is open exactly 8", no more and no less.
This
aperture is important for proper airflow.
If the viewscreen is at the wrong height, an
alarm will sound.
7. After the cabinet has operated for at least
three minutes with the window in the proper
position, you are ready to begin.
Working in the Cabinet Work Space
1. Hands and arms should be washed
thoroughly with germicidal soap both before
and after work in the cabinet. Operators are
encouraged to wear long-sleeved govvns or
lab coats with tight-fitting cuffs and sterile
gloves. This minimizes the shedding of skin
flora into
the work area and protects hands
and arms from contamination
2. Perfonn all work on the depressed area of the
solid work surface. Work with a limited
number of slow movements. Since all of the
equipnlent you need is already in tl1e cabinet,
it \Vili not be necessary to move your arms in
and out through the air barrier.
3. Because opening and closing doors in the
laboratorv causes air disturbcmce wluch
might interfere with cabinet airflow, this kind

of activity should be kept to a minimum
while the cabinet is in use.
4. Avoid using floor-type pipette discard
canisters.
It is important that your used
pipettes be discarded onto a tray or other
suitable container inside the cabinet. This
reduces the temptation to move in and out of
the work area unnecessarily.
Because of tl1e restricted access, pipetting
within the cabinet will require the use of
pipett:ing
aids.
5. Use good aseptic technique. Procedures done
with good technique and proper cabinet
methods will not require the use of a flame.
If, however, a safety officer approves the use
of flame after evaluating the circumstances,
then a burner with a pilot ljght such as the
"Touch-0-Matic" should be used. Place it at
the rear of the work area where the air
turbulence caused by the flame wilt have the
least possible effect. Flame disturbs the
directional airstream and also contributes to
tile heat load. If the cabinet blower is
unintentionally turned off, the flame could
also damage a filter.
Tubing for a burner within the cabinet should
be resistant to cracking or pU11cture. Material
such as Tygon tubing may not be acceptable
for this use.
6. Never operate your cabinet while the
viezvscreen alarm iHdicator is on. The
operating position of the sash provides an 8-
inch high access opening. This restricted
opening permits optimum operating
cuHditiou:; foL the cabinet. For operati.ttg
comfort it is recorrunended that the top of the
operator's shoulder be at the same height as
the bottom of the viewscreen. Because
operators wili not an be the same height, it is
suggested that the operator use a chair which
may be adjusted for height.
7. ~-\fter a procedure has been cornpleted, all
equipn1ent 1-vhich has been !_n contact \Vith
the research agent should be enclosed, and
the entire surface decontarninated. Trays of
discarded pipettes artd glassv..'are should be
covered. The cabinet should then be allowed
14
to nm for at least three minutes with no
activity so that the airborne contaminants will
be purged from tbe work area. Next, make
sure that all equipment is removed from the
cabinet.
8. After you have removed all materials, culture
apparatus, etc., decontamination of the
interior surfaces should be repeated. Check
the work area carefully for spilled or splashed
nutrient which might support bacterial
growth. Never use the cabinet to store
supplies or laboratory equipment.
9. We recommend that the cabinet be left
numing continuously to ensure containment
and cleanliness. If the user elects to turn the
cabinet off at the end of a work session, the
viewscreen should be closed completely. The
sash alaml will be silenced when the
viewscreen is in the closed position.
Reacting to Spills
1. If an accident occurs which causes spills and
spatters arolll1d the work area, you will need
to decontaminate all items and surfaces
before any items are removed. If the spill
was enough to create puddles of liquid in the
drain pan, then an emergency spiU procedure
should be followed. (This procedure should
be established prior to an accident.)
It
is recommended that the researchers, in
coordination with their consulting safety
professional, have a written plan available in
case of an accidental exposure or spill. The
safety p!Bn shoHld include all of the
ernergency procedu1es to be folloYved in t}'te
event of an accident. All employees should
be familiar with the emergency procedures.
The emergencv spill procedure may vary
accord.ing to the agents being used.
2. In the case of a biological spill, for example,
the area containing the spi_U n1ay be flooded
with an appropriate disinfectant. The drain
capacity of the SG400 rnodels is 19.S gallons,
with the SG600 mode.\s having a 28.7 gal.lon
capacity.
iJter the disirlfectant has had tllne for a
complete kill, remove or drain tbe residue. If

you have used a disinfectant which is
harmful to stainless steel (Hypochlorite
solutions, for example) be sure that none
remains to corrode cabinet surfaces. Clean
the s>Jrfaces with sterile water.
3. If you have a spill involving a hazardous
Biosafety Level 2 or 3 agent, then you are
advised to leave the cabinet running and
close the view screen, so as to let the aerosols
settle before you start cleanup procedures.
With some spills, it may be necessary to
decontaminate the room with an ag~nt such
as formaldehyde gas. (Biosafety Level 4
agents should never be used in this type of
cabinet.) ,
4. If the spill contains volatile liquids which
generate vapors creating a danger of fire or
explosion, tum off the unit and other
electrical appliances and close the viewscreen.
Evacuate and seal tl1e room and call for
irrunediate help from a safety professional.
5. lf the agent is a hazardous chemical, it may
be recommended that a Spill Kit be kept
readily available. This kit should be clearly
labeled, and might include such items as a
respirator, chemical splash goggles, two pairs
of gloves, two sheets of absorbent material,
spill control pillows, a solution to clean the
contaminated area, and waste disposal bags
or other conta.iners. Consult your safety
professional for proper procedures and
treatment of the specific agents you plan to
use.
Ultraviolet (Germicidal) Light
This SterilGARD"II unit has an ultraviolet light.
The light is controlled by an on/ off switch on the
front panel. See Fig. 5. \Nhen the fluorescent light
rs on, the ultraviolet light cannot operate. The ev
light \VilJ not operate unless the vievvscreen is
cornpleteJy dovv'n and t11e fluorescent light is off.
Cltraviolet lamps lose their effectiveness 0'/er
tirr1e and should be replaced vvhen _inten::it\.r droD~
be.! ow tile optimum le,vel. Check regular.ly·. ' -
15
. WA.RNLNG!
• Eyes and ski11 should not be exposed to
dired ultraviolet light.
• Ultraviolet
light should not be relied upon
as . t~re sole decontamiltating agent.
Addtiwnal sulface disinfection should be
performed
both before and after every
cabinet usc.
• A biological safety cabinet acts as a sup-
plement to good aseptic practices, 110t as a
replacement.
Decontamination
vVhenever maintenance, service or repair is
needed in a contaminated area of vour cabinet
the unit must first be deconta:min~ted bv an '
appropriate agent. The National InstitL;te of
Health, ~ational Cancer Institute and the Center
for Disease Control have all recommended the use
of formaldehyde gas for most microbiological
agents. Its
application requires individuals who
are experienced in the decontamination of
cabinets, since the gas itself is toxic.
A good reference for this procedure is The
National Cancer Institute's "Formaldehyde
Decontamination of Laminar Flow Biological
Safety Cabinets"
(pamphlet and/ or slide
program), U.S. Department of Health, Education
and Welfare: National Institutes of Health.
Available
through Chief of Sales Branch, '\Jational
Audiovisual Center, Washi11gton, DC 20409.
An ethylene oxide gas mixture is an alternative,
but it involves a more complicated procedure and
should only be used by personnel ';.Vho are
familiar with its operation.
Whatever gas you choose, have the proper
safety equiprnent (gas masksr protective clothin(',
t ) . l . b
e c. \Nlt uneasy reach. ln addition, vou vvilJ vvant
to be sure that the gas you are using.will be
effective against all of the biolcsical agents \vithin
the cabinet V\
1
hen you have dt;cid_ed ~rlrich gas to
use, post the antidote to it in a visible and n~~trb\r
location. The volurne of the SG-100 cabinet is 55 ·
cubic feet ,_::ubic feet- for the SG600 L Pruvkie
tl1e correct arnowTt of deco.ntarrtinating gtts for
tbis volurne. ,